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EC Tree
The taxonomic range for the selected organisms is: Solanum lycopersicum The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
beta-mannanase, endo-beta-mannanase, man5a, endo-mannanase, manb-1601, endo-beta-1,4-mannanase, man26a, man26b, man5c, endo-1,4-beta-mannanase,
more
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(1,4)-beta-D-mannan mannanohydrolase
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1,4-beta-D-mannan mannanohydrolase
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beta-1,4-mannan 4-mannanohydrolase
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-
-
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endo-1,4-beta-mannanase
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endo-1,4-mannanase
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endo-beta-1,4-mannase
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endo-beta-D-mannanase
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mannanase, endo-1,4-beta-
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endo-beta-mannanase
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hydrolysis of O-glycosyl bond
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4-beta-D-mannan mannanohydrolase
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galactomannan + H2O
?
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?
glucomannan + H2O
?
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-
-
?
Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose
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?
azurine-crosslinked galactomannan + H2O
?
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hydrolysis
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?
galactoglucomannan + oligosaccharide
[3H]-labelled polysaccharide
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transglucosylation
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-
?
Manbeta(1-4)Manbeta(1-4)Man + H2O
?
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-
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?
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9
Manbeta(1-4)Manbeta(1-4)Man
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additional information
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4.6 - 4.7
calculated from amino acid sequence
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Swissprot
brenda
tomato
Swissprot
brenda
var. Mill.
Swissprot
brenda
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brenda
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brenda
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brenda
additional information
not in leaf
brenda
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brenda
red fruit, not during early stages of ripening
brenda
isoform Man4a is expressed in the fruit cell wall at all ripening stages, but it is not active during the initial green stage. This is not due to the presence of inhibitors of its activity, nor due to changes in its mRNA sequence
brenda
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brenda
transient expression in onion epidermal cells of endo-beta-mannanase trancripts fused to green fluorescent protein results in the expressed enzyme being localized to the cell walls. This occurs initially during ripening in the skin and outer pericarp of the fruit, and later in the skin and throughout the pericarp
brenda
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brenda
main enzyme activity in the fruit
brenda
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physiological function
when endo-beta-mannanase activity is much reduced by RNAi and antisense RNA strategies, their firmness is higher compared to those of control fruits at the turning and orange-color stages, but at the red-ripe stage firmness is similar between the two fruit-types
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MAN4_SOLLC
399
0
45339
Swiss-Prot
Secretory Pathway (Reliability: 1 )
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38950
calculation from sequence of DNA
42400
x * 42400, deduced from gene sequence, mature protein
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?
x * 42400, deduced from gene sequence, mature protein
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proteolytic modification
amino acid sequence contains a signal peptide cleavage site between residues 21-22, targeting sequence for cell wall
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hanging drop vapor diffusion
molecular dynamic simulation of wild-type and mutant lacking the C-terminal amino acid residues 394-399, SerLysLeuSer. The inactive form has a lower stability than the active one. The loss of amino acids from the C-terminal end of the protein indirectly affects the conformation of the catalytic Glu318 residue and stability of active site because of interactions between residues at the C-terminus and the rest of protein
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additional information
the cultivar Walter displays an inactive enzyme due to the absence of the C-terminal four amino acid residues 394-399, SerLysLeuSer. The inactive form has a lower stability than the active one. The loss of amino acids from the C-terminal end of the protein indirectly affects the conformation of the catalytic Glu318 residue and stability of active site because of interactions between residues at the C-terminus and the rest of protein
additional information
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the cultivar Walter displays an inactive enzyme due to the absence of the C-terminal four amino acid residues 394-399, SerLysLeuSer. The inactive form has a lower stability than the active one. The loss of amino acids from the C-terminal end of the protein indirectly affects the conformation of the catalytic Glu318 residue and stability of active site because of interactions between residues at the C-terminus and the rest of protein
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-20°C, MES buffer, 4 months, without loss of activity
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4°C, 48h, 20% residual activity
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cation exchange chromatography
salt extraction, dialysis, cation exchange chromatography
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expressed in Escherichia coli strain BL21
expressed in Arabidopsis thaliana transformed with Agrobacterium tumefaciens
expressed in Escherichia coli
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biotechnology
bio-bleaching for kraft pulp production
agriculture
fruit ripening and seed germination
agriculture
when endo-beta-mannanase activity is much reduced by RNAi and antisense RNA strategies, their firmness is higher compared to those of control fruits at the turning and orange-color stages, but at the red-ripe stage firmness is similar between the two fruit-types
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Groot, S.P.C.; Kieliszewska-Rokicka, B.; Vermeer, E.; Karsson, C.M.
Gibberellin-induced hydrolysis of endosperm cell walls in gibberellin-deficient tomato seeds prior to radicle protrusion
Planta
174
500-504
1988
Solanum lycopersicum
brenda
Bewley, J.D.; Burton, R.A.; Morohashi, Y.; Fincher, G.B.
Molecular cloning of a cDNA encoding a (1->4)-beta-mannan endohydrolase from the seeds of germinated tomato (Lycopersicon esculentum)
Planta
203
454-459
1997
Solanum lycopersicum (Q8L5J1), Solanum lycopersicum
brenda
Carrington, C.M.S.; Vendrell, M.; Dominguez-Puigjaner, E.
Characterisation of an endo-(1,4)-b-mannanase (LeMAN4) expressed in ripening tomato fruit
Plant Sci.
163
599-606
2002
Solanum lycopersicum (Q8L5J1)
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brenda
Filichkin, S.A.; Leonard, J.M.; Monteros, A.; Liu, P.P.; Nonogaki, H.
A novel endo-beta-mannanase gene in tomato LeMAN5 is associated with anther and pollen development
Plant Physiol.
134
1080-1087
2004
Solanum lycopersicum (Q6YM50), Solanum lycopersicum
brenda
Schroeder, R.; Wegrzyn, T.F.; Sharma, N.N.; Atkinson, R.G.
LeMAN4 endo-beta-mannanase from ripe tomato fruit can act as a mannan transglycosylase or hydrolase
Planta
224
1091-1102
2006
Solanum lycopersicum
brenda
Bourgault, R.; Oakley, A.J.; Bewley, J.D.; Wilce, M.C.
Three-dimensional structure of (1,4)-beta-D-mannan mannanohydrolase from tomato fruit
Protein Sci.
14
1233-1241
2005
Solanum lycopersicum (Q8L5J1), Solanum lycopersicum
brenda
Li, J.; Bewley, J.D.; Hua, Z.; Zheng, W.; Wang, A.
Model and molecular dynamic simulations of active and inactive endo-beta-1,4-mannanase in tomato fruit
Protein J.
27
363-370
2008
Solanum lycopersicum (Q8L5J1), Solanum lycopersicum
brenda
Wang, A.; Li, J.; Zhang, B.; Xu, X.; Bewley, J.D.
Expression and location of endo-beta-mannanase during the ripening of tomato fruit, and the relationship between its activity and softening
J. Plant Physiol.
166
1672-1684
2009
Solanum lycopersicum (Q8L5J1), Solanum lycopersicum
brenda