Information on EC 3.2.1.77 - mannan 1,2-(1,3)-alpha-mannosidase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.2.1.77
-
RECOMMENDED NAME
GeneOntology No.
mannan 1,2-(1,3)-alpha-mannosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->2)- and (1->3)-linkages in yeast mannan, releasing mannose
show the reaction diagram
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-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Fructose and mannose metabolism
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SYSTEMATIC NAME
IUBMB Comments
(1->2)-(1->3)-alpha-D-mannan mannohydrolase
A 1,6-alpha-D-mannan backbone remains after action on yeast mannan. This is further attacked, but slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
37288-53-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
M-30
-
-
Manually annotated by BRENDA team
M-30
-
-
Manually annotated by BRENDA team
M-25
-
-
Manually annotated by BRENDA team
M-25
-
-
Manually annotated by BRENDA team
M-15
-
-
Manually annotated by BRENDA team
M-15
-
-
Manually annotated by BRENDA team
M-03
-
-
Manually annotated by BRENDA team
M-03
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
mannan + H2O
?
show the reaction diagram
mannan + H2O
mannose + 1,6-alpha-D-mannan-backbone
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
mannan + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
restores activity after EDTA treatment
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
Mannan
-
based on molecular weight of 22000 from intact S. cerevisiae mannan
0.5 - 2
oligosaccharides from mannan
-
molecular weight of mannan multiplied by factor 50 describes molar concentration of side chains in the mannan molecule
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
-
mannan
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8.5
-
-
5 - 9
-
mannan
5 - 7.5
-
-
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
completely inactivated
70
-
not stable above
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10C, indefinitely,
-
0C, unstable on storage
-
20C, one drop of toluene, 1 day, 15% decline in activity
-
4C, 2 days,
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partially
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