Information on EC 3.2.1.74 - glucan 1,4-beta-glucosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.74
-
RECOMMENDED NAME
GeneOntology No.
glucan 1,4-beta-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->4)-linkages in (1->4)-beta-D-glucans, to remove successive glucose units
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
4-beta-D-glucan glucohydrolase
Acts on 1,4-beta-D-glucans and related oligosaccharides. Cellobiose is hydrolysed, but very slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
37288-52-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain 155 v cellulolytic fungus
-
-
Manually annotated by BRENDA team
strain 155 v cellulolytic fungus
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
ATCC35296
Uniprot
Manually annotated by BRENDA team
ATCC35296
Uniprot
Manually annotated by BRENDA team
Clostridium stercorarium
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Gilmaniella humicola
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
BPR2001
-
-
Manually annotated by BRENDA team
BPR2001
-
-
Manually annotated by BRENDA team
Myrothecium striatisporum
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
subsp. cellulosa
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
AR67
-
-
Manually annotated by BRENDA team
Sporotrichum pulverulentum
also called Chrysosporium lignorum
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Thermomonospora sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
QM 9414
-
-
Manually annotated by BRENDA team
ZU-02
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
strain E1 Tor N16961
-
-
Manually annotated by BRENDA team
Vibrio cholerae E1 Tor N16961
strain E1 Tor N16961
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenol cellobiose + H2O
?
show the reaction diagram
-
-
-
?
2-nitrophenyl beta-cellobioside + H2O
2-nitrophenol + beta-cellobiose
show the reaction diagram
-
3.1% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
show the reaction diagram
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucopyranose
show the reaction diagram
4-methylumbelliferryl 4-O-(beta-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-cellobioside + H2O
?
show the reaction diagram
4-methylumbelliferryl-beta-cellotrioside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-D-cellotrioside + H2O
?
show the reaction diagram
-
-
-
?
4-methylumbelliferryl-beta-D-glucopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-D-glucoside + H2O
?
show the reaction diagram
4-methylumbelliferyl beta-D-cellobioside + H2O
D-glucose + ?
show the reaction diagram
i.e.MuC, 14.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate
-
-
?
4-methylumbelliferyl-beta-D-cellobioside + H2O
?
show the reaction diagram
-
-
-
?
4-methylumbelliferyl-beta-D-cellobioside + H2O
D-glucose + ?
show the reaction diagram
i.e.MuC, 157.7% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate
-
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
4-methylumbelliferone + D-lactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-cellobioside + H2O
4-nitrophenol + beta-cellobiose
show the reaction diagram
-
6.5% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
show the reaction diagram
-
exo-beta-1,4-glucanase activity, 24.1% activity compared to carboxymethylcellulose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-
1.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranose + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
show the reaction diagram
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
acid swollen cellulose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
?
acid-swollen cellulose + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
alpha-cellulose + H2O
?
show the reaction diagram
-
no activity with carboxymethylcellulose, salicin, cellobiose and alpha-dimethylglucoside
-
-
?
amorphous cellulose + H2O
?
show the reaction diagram
-
-
-
?
amygdalin + H2O
?
show the reaction diagram
-
-
-
-
?
arbutin + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
avicel + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
avicel + H2O
cellobiose + ?
show the reaction diagram
-
0.09% activity compared to carboxymethylcellulose
-
-
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
show the reaction diagram
bacterial cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
bacterial microcrystalline cellulose + H2O
?
show the reaction diagram
-
-
-
?
barley glucan + H2O
?
show the reaction diagram
-
326% activity compared to carboxymethylcellulose
-
-
?
beta-1,4-xylan + H2O
?
show the reaction diagram
-
-
-
-
?
beta-glucan + H2O
?
show the reaction diagram
-
from yeast and from barley
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
carboxymethyl cellulose + H2O
cellobiose + cellopentaose + cellotetraose + cellotriose + D-glucose
show the reaction diagram
-
-
the major product is cellobiose with some minor products
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
carboxymethylcellulose + H2O
D-glucose + cellooligosaccharides
show the reaction diagram
Thermomonospora sp.
-
-
-
-
?
cellobiose + H2O
2 beta-D-glucose
show the reaction diagram
cellobiose + H2O
2 D-glucose
show the reaction diagram
cellobiose + H2O
D-glucose
show the reaction diagram
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
cellodextrins + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
celloheptaose + H2O
?
show the reaction diagram
cellohexaose + H2O
cellopentaose + D-glucose
show the reaction diagram
-
-
-
?
cellohexaose + H2O
D-glucose
show the reaction diagram
cellooligomers + H2O
cellobiose + cellooligomers
show the reaction diagram
-
-
-
ir
cellooligomers + H2O
D-glucose
show the reaction diagram
cellooligomers + H2O
D-glucose + cellooligomers
show the reaction diagram
-
-
-
ir
cellopentaose + H2O
?
show the reaction diagram
cellopentaose + H2O
D-glucose
show the reaction diagram
cellopentaose + H2O
glucose
show the reaction diagram
cellotetraose + 3 H2O
4 D-glucose
show the reaction diagram
cellotetraose + H2O
?
show the reaction diagram
cellotetraose + H2O
cellobiose + cellotriose + D-glucose
show the reaction diagram
-
-
cellobiose is the dominant product, while cellotriose and glucose are minor products
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
cellotetraose + H2O
glucose + cellobiose + cellotriose
show the reaction diagram
-
with a conversion ratio of 80%
-
?
cellotriose + H2O
D-glucose
show the reaction diagram
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
cellotriose + H2O
glucose
show the reaction diagram
cellulose + H2O
?
show the reaction diagram
cellulose + H2O
D-glucose
show the reaction diagram
cellulose + H2O
D-glucose + ?
show the reaction diagram
cellulose acetate + H2O
?
show the reaction diagram
-
-
-
-
?
cotton + H2O
?
show the reaction diagram
-
-
-
-
?
D(+)-cellobiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
r
esculin + H2O
?
show the reaction diagram
-
-
-
-
?
filter paper
?
show the reaction diagram
-
-
-
?
filter paper + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
filter paper + H2O
cellobiose + ?
show the reaction diagram
-
0.02% activity compared to carboxymethylcellulose
-
-
?
filter paper + H2O
cellobiose + cellopentaose + cellotetraose + cellotriose + D-glucose
show the reaction diagram
-
-
the major product is cellobiose with some minor products
-
?
gentiobiose + H2O
?
show the reaction diagram
hydroquinone-beta-D-glucopyranoside + H2O
hydroquinone + D-glucose
show the reaction diagram
-
-
-
?
laminaribiose + H2O
?
show the reaction diagram
laminarin + H2O
?
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
?
show the reaction diagram
-
from barley
-
-
?
lichenan + H2O
?
show the reaction diagram
n-heptyl-beta-D-glucopyranoside + H2O
n-heptanol + D-glucose
show the reaction diagram
-
-
-
?
n-hexyl-beta-D-glucopyranoside + H2O
n-hexanol + D-glucose
show the reaction diagram
-
-
-
?
n-octyl-beta-D-glucopyranoside + H2O
n-octanol + D-glucose
show the reaction diagram
-
-
-
?
n-pentyl-beta-D-glucopyranoside + H2O
n-pentanol + D-glucose
show the reaction diagram
-
-
-
?
o-nitrophenyl beta-D-glucopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranose + H2O
o-nitrophenol + beta-D-galactopyranose
show the reaction diagram
p-nitrophenol beta-D-cellobioside + H2O
p-nitrophenol beta-D-glucopyranoside + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl beta-D-lactoside + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl cellobioside + H2O
p-nitrophenyl + glucose
show the reaction diagram
p-nitrophenyl glucoside + H2O
p-nitrophenyl + glucose
show the reaction diagram
-
p-nitrophenyl releasing with no lag period, no activity with p-nitrophenyl-?-(N-acetylgalactosamine), p-nitrophenyl-?-glucose-6-phosphate, p-nitrophenyl-(N-acetylglucosamine), p-nitrophenyl-di(N-acetylglucosamine), p-nitrophenyl-tri(N-acetylglucosamine)
-
-
?
p-nitrophenyl-beta-D-cellobiose + H2O
p-nitrophenol + cellobioside
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
cellobiose + p-nitrophenol
show the reaction diagram
-
-
-
ir
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
p-nitrophenyl-beta-D-cellotrioside + H2O
D-glucose + p-nitrophenyl-beta-D-cellobioside + p-nitrophenyl-beta-D-cellotetraoside + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
p-nitrophenyl-beta-D-fucopyranoside + H2O
p-nitrophenol + fucopyranose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
p-nitrophenyl-cellobioside + H2O
?
show the reaction diagram
-
-
-
-
?
phenyl-beta-D-glucopyranoside + H2O
phenol + D-glucose
show the reaction diagram
-
-
-
?
phosphate acid swollen cellulose + H2O
?
show the reaction diagram
-
10.7% activity compared to carboxymethylcellulose
-
-
?
prunasin + H2O
D-mandelonitrile + beta-D-glucose
show the reaction diagram
-
-
-
-
?
salicin + H2O
?
show the reaction diagram
sigmacell + H2O
?
show the reaction diagram
-
-
-
-
?
sophorose + H2O
?
show the reaction diagram
sugarcane bagasse + H2O
?
show the reaction diagram
swollen cellulose + H2O
cellobiose + cellotriose
show the reaction diagram
-
-
-
?
trehalose + H2O
?
show the reaction diagram
xylan + H2O
?
show the reaction diagram
xylo-oligosaccharides + H2O
?
show the reaction diagram
-
-
-
-
?
xylobiose + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cellobiose + H2O
D-glucose
show the reaction diagram
-
hydrolysis of cellulose
-
-
ir
cellulose + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
5 mM, 107.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 92.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Co2+
-
5 min at 50C, 10 mM, 101.9% of the relative activity without metal ions; 5 min at 50C, 1 mM, 103.0% of the relative activity without metal ions
CoCl2
5 mM, 79.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 85.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
CuCl2
5 mM, 79.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 83.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-fructose
5 mM, 102.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-glucose
5 mM,82% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-xylose
5 mM, 106.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Fe3+
-
5 min at 50C, 10 mM, 103.6% of the relative activity without metal ions; 5 min at 50C, 1 mM, 105.5% of the relative activity without metal ions
fructose
5 mM, 107.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
glucose
5 mM,24.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
MgCl2
5 mM, 110.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 96.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Mn2+
-
5 min at 50C, 10 mM, 104.1% of the relative activity without metal ions; 5 min at 50C, 1 mM, 105.8% of the relative activity without metal ions
NiCl2
5 mM, 76.7% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 86.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
xylose
5 mM, 88% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
ZnSO4
5 mM, 111.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 90.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-anhydro-myo-inositol
-
-
Ag+
-
5 min at 50C, 10mM, 100.0% of inhibition; 5 min at 50C, 1 mM, 100.0% inhibition
D-cellobiose
D-glucono-1,5-lactone
-
-
D-glucose
EGTA
-
10% of enzyme inhibition at 20 mM, at 37C, pH 6.5
Fe3+
-
80.5% residual activity at 10 mM
iodoacetoamide
KCl
-
25% of enzyme inhibition at 0.5 mM, at 37C, pH 6.5
Mg2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Mg2+
N-bromosuccinimide
Ni2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ni2+
o-phthaldialdehyde
Thermomonospora sp.
-
-
p-chloromercuribenzoate
-
-
xylobiosyl-deoxynojirimycin
-
X2DNJ
xylobiosyl-isofagomine
-
X2IF
xylobiosyl-isofagomine lactam
-
X2IL
additional information
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ethanol
methanol
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.74
4-nitrophenyl beta-D-cellobioside
-
at 65C in pH 5.5 citrate buffer
0.22 - 2.5
4-nitrophenyl beta-D-glucopyranoside
0.65
alpha-cellulose
-
pH 5.0
-
0.461 - 86
cellobiose
0.028 - 0.73
cellohexaose
0.37 - 6
Cellopentaose
0.77 - 6.7
Cellotetraose
0.44 - 8.7
cellotriose
127
cellulose
-
H3OP4 swollen
10.7
gentiobiose
-
-
0.77
o-nitrophenyl beta-D-glucopyranoside
-
-
0.45
p-nitrophenyl glucoside
-
pH 6.5, at 37C
0.7
p-nitrophenyl-beta-D-cellobioside
2.8 - 4
p-nitrophenyl-beta-D-glucopyranoside
0.42 - 2.2
p-nitrophenyl-beta-D-glucoside
additional information
additional information
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00039
2,4-dinitrophenol cellobiose
Thermobifida fusca
Q9XCD4
pH 5.5
69.6
4-nitrophenyl beta-D-cellobioside
uncultured bacterium
-
at 65C in pH 5.5 citrate buffer
126.6 - 4945
4-nitrophenyl beta-D-glucopyranoside
0.25 - 281
cellobiose
16.3
cellohexaose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
16.4
Cellopentaose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
18.6
Cellotetraose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
6.21
cellotriose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
40.1
4-nitrophenyl beta-D-cellobioside
uncultured bacterium
-
at 65C in pH 5.5 citrate buffer
4692
11900 - 915700
4-nitrophenyl beta-D-glucopyranoside
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
29
D-glucose
-
in 100 mM sodium acetate buffer (pH 4.5), at 65C
0.0058 - 0.18
xylobiosyl-deoxynojirimycin
0.00013 - 0.0071
xylobiosyl-isofagomine
0.00034 - 0.024
xylobiosyl-isofagomine lactam
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0011
-
P-42, cotton, pH 5, at 30C
0.0012
-
P-42, avicel, pH 5, at 30C
0.0021
-
P-42, bacterial cellulose, pH 5, at 30C
0.0037
Clostridium stercorarium
-
-
0.0142
-
Ex-1, cotton, pH 5, at 30C
0.0145
-
Ex-1, bacterial cellulose, pH 5, at 30C
0.0209
-
Ex-1, avicel, pH 5, at 30C
0.0243
-
Ex-1, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30C
0.0244
-
P-42, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30C
0.025
Clostridium stercorarium
-
cellulose MN300
0.026
-
Ex-1, carboxymethyl cellulose, pH 5, at 30C
0.03
Clostridium stercorarium
-
cellulose HBS
0.0311
-
P-42, carboxymethyl cellulose, pH 5, at 30C
0.04
-
using filter paper as substrate, at 60C in pH 6.5 citrate buffer
0.041
Clostridium stercorarium
-
avicel
0.067
-
crude enzyme from homogenate, using cellobiose as substrate, pH and temperature not specified in the publication
0.102
Clostridium stercorarium
-
H3PO4 swollen avicel
0.108
Clostridium stercorarium
-
HCl swollen avicel
0.128
Clostridium stercorarium
-
celloheptaose
0.2
-
using avicel as substrate, at 60C in pH 6.5 citrate buffer
0.228
-
after 3.4fold purification, using cellobiose as substrate, pH and temperature not specified in the publication
0.236
Clostridium stercorarium
-
cellohexaose
0.323
-
pH 6.5, at 37C
0.34
Clostridium stercorarium
-
xylan
0.35
2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 40C
0.42
Clostridium stercorarium
-
cellopentaose
0.65
2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 42C
0.684
Clostridium stercorarium
-
cellotetraose
1.9
-
sigmacell, pH 5.0
2.53
-
cellulose acetate, pH 5.0
3.36
-
cellulose, pH 5.0
3.583
-
alpha-cellulose, pH 5.0
5.3
cellobiose as substrate, pH 4.6, 42C
11.5
4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 42C
14.5
-
bacterial cellulose powder, not dried, isoenzyme II
15.1
-
bacterial cellulose powder, not dried, isoenzyme I
20.9
-
avicel, isoenzyme II
21.1
-
avicel, isoenzyme I; isoenzyme I
23.5
-
using phosphate acid swollen cellulose as substrate, at 60C in pH 6.5 citrate buffer
25.5
-
carboxymethyl cellulose, isoenzyme I
27.1
-
p-nitrophenyl beta-D-lactoside, isoenzyme II
27.3
-
p-nitrophenyl beta-D-lactoside, isoenzyme I
33.2
-
p-nitrophenyl beta-D-celloside, isoenzyme II
33.5
cellobiose as substrate, pH 4.6, 40C
34
-
p-nitrophenyl beta-D-celloside, isoenzyme I
41
4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 40C
52.9
-
using 4-nitrophenyl beta-D-cellobioside as substrate, at 65C in pH 5.5 citrate buffer
58.79
Thermomonospora sp.
-
carboxymethylcellulase activity
77
4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
129
p-nitrophenyl-beta-D-fucopyranoside as substrate
130
o-nitrophenyl-beta-D-galactopyranoside as substrate; p-nitrophenyl-beta-D-cellobioside as substrate
133
p-nitrophenyl-beta-D-galactopyranoside as substrate
177
p-nitrophenyl-beta-D-glucopyranoside as substrate
220
-
using carboxymethylcellulose as substrate, at 65C in pH 5.5 citrate buffer
693
-
using lichenan as substrate, at 60C in pH 6.5 citrate buffer
717
-
using barley glucan as substrate, at 60C in pH 6.5 citrate buffer
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6
assay at, optimal for 4-nitrophenol beta-D-glucopyranoside as substrate; assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside as substrate
5 - 6
Clostridium stercorarium
-
-
5.5 - 6.5
-
using 4-nitrophenyl beta-D-cellobioside as substrate
6 - 6.5
-
-
7
Thermomonospora sp.
-
for both cellulase and xylanase activity
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 5
-
84% and 86% of the maximum activity appears at pH 4.0 and 5.0, respectively
5 - 9
retaines more than 50% of activity, 50C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 42
-
-
42
assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside as substrate
85
-
avicelase activity
95 - 100
-
carboxymethyl cellulase activity
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 37
-
stable for at least 1 h
40
-
incubation for 3 h, 40% loss of enzyme activity
60 - 70
-
85% and 91% of the maximum activity at 60C and 70C, respectively
70
-
incubation for 3 h, 100% loss of enzyme activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.33
Thermomonospora sp.
-
isoelectric focusing
4.9
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
Driselase, from Irpex lacteus
Manually annotated by BRENDA team
Thermomonospora sp.
-
-
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
expressed in Escherichia coli
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
42000
-
P-42, SDS-PAGE
48600
Sporotrichum pulverulentum
-
ultracentrifugation
56000
-
isoenzyme I
62000
-
calculation from sequence of amino acids
64000
-
gel filtration
88000
-
SDS-PAGE
130300
-
gel filtration
151000
-
gel filtration
169300
-
calculated from amino acid sequence
200000
-
gel filtration
780000
-
gel filtration
additional information
-
70000 g/mol
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homooctamer
additional information
Thermomonospora sp.
-
fluorescence study on interactions of alpha-crystallin with the molten globule state of the enzyme induced by guanidine hydrochloride
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
no modification
proteolytic modification
-
Papain digestion of native Ex-1, core fragment obtaining
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
resolution 2.0 A
-
combination of the immunoglobulin-like module and the catalytic module and complex of inactive mutant with cellotetraose, modified microbatch crystallization under oil at 0C
-
molecular docking studies for the enzyme with the substrates cellobiose and 4-nitrophenyl-beta-D-glucopyranoside. The essential catalytic residues of RuBG3A are D297 and E499. Besides D113, K218, H219, and R185 also form hydrogen bonds with substrates and are condervative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters; molecular docking studies for the enzyme with the substrates cellobiose and 4-nitrophenyl beta-D-glucopyranoside. The essential catalytic residues of RuBG3B are D272 and E481. Besides D88, K193, H194, and R160 also form hydrogen bonds with substrates and are conservative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 5.5
-
after 24 h of incubation at 20C, the enzyme is fairly stable at the acidic pH ranging from 3.0 to 5.5. About 15% and 76% of activity remain after incubation at pH 2.0 and 2.5, respectively
713875
3 - 5
-
isoenzyme II
136834
3 - 7
-
Ex-1, retains more than 80% of its maximum activity
656306
3 - 9
-
isoenzyme I
136834
3.6 - 5.7
-
analysis of influence of sodium citrate buffer pH
655281
3.6 - 7
-
-
136832
5 - 5.5
-
for 1 h, at 50C
654146
5 - 8
when incubated at 40C for 12 h with no substrate
677938
5 - 6
-
P-42, retains more than 70% of its maximum activity
656306
5 - 8
Thermomonospora sp.
-
50C, 1 h, less than 15% loss of activity
663865
5.5 - 8
-
-
136845
6 - 8
retains 80% activity
698578
6 - 7.5
-
most stable at pH 6-7.5
136826
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25 - 40
-
P-42, pH 5.0, retains more than 80% of its maximum activity
25 - 55
-
Ex-1, pH 5.0, retains more than 80% of its maximum activity
30 - 45
-
for 1 h, pH 5.2
40 - 60
-
loses none of its activity for a period of up to 5 h
40 - 50
decreases markedly above 50C
45
40% activity retained after incubation for 1 h; stable after 1 h incubation
50
-
both isoenzymes stable below 50C
50 - 70
-
at 70C, 65C, 60C, and 50C the enzyme retains 50% of the initial activity after 2, 10, 16, and 35 min, respectively
55
-
stable below 55C
60
-
the melting temperature of the enzyme is at 60C
70
-
undergoes considerable deactivation within quite a short time
80
Thermomonospora sp.
-
half-life: 20 min
95
-
50% activity loss after 30 min at 95C
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ethanol
5% (v/v), 100.8% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5% (v/v), 158.4% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42C
Methanol
5% (v/v), 108.2% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5% (v/v), 141% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C or -80C, 0.5 mM dithiothreitol
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
12fold
Clostridium stercorarium
-
15fold
Sporotrichum pulverulentum
-
ammonium sulfate precipitation, Macro-Prep DEAE column chromatography, methyl hydrophobic-interaction column chromatography, and Superdex 200 gel filtration
-
by nickel affinity chromatography and gel filtration chromatography
-
catalytic and cellulose binding domains by cellulose FPLC column chromatography, ultrafiltration and papain treatment
-
centrifugation, streptomycin sulfate precipitation, ammonium sulfate fractionation, DEAE column chromatography, IMAC-Zn2+ chromatography
-
cloned Cel48A, centrifugation, phenylmethanesulfonyl fluoride and ammonium sulfate fractionation, phenyl-Sepharose column, Q-Sepharose column; native Cel48A, HPLC
gel filtration
-
Ni-nitrilotriacetatic acid agarose column chromatography
Ni-NTA resincolumn chromatography
-
precipitation with acetone, gel filtration chromatography on Sephadex G-100 and DEAE-cellulose chromatography, 47.14fold
-
recombinant enzyme; recombinant enzyme
Superdex 200 gel filtration
-
TSC by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration
Thermomonospora sp.
-
wild-type and mutants purified by immobilized metal affinity chromatography. Wild-type further purified by gel filtration
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Escherichia coli
Escherichia coli and Streptomyces lividans
-
Escherichia coli and Streptomyces lividans, expression in Streptomyces lividans
expressed in Escherichia coli and purified; expressed in Escherichia coli and purified
expressed in Escherichia coli BL21(DE3) cells
-
expression in Escherichia coli
into vector pET30a and expressed in Escherichia coli BL21(DE3)
overexpression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N44A
-
based on the pUC12-1.1Cex PTIS plasmid
Q87M
-
based on the pUC12-1.1Cex PTIS plasmid
Q87Y
-
based on the pUC12-1.1Cex PTIS plasmid
D166A
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
DELTAS168
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
E612A
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
S168A
retains about 40% of activity compared to the wild-type
E795Q
-
inactive enzyme
A229V
-
loop mutant, weaker binding affinities to all tested ligands
E495D
-
active site mutant, weaker binding affinities to all tested ligands
E495N
-
active site mutant, weaker binding affinities to all tested ligands
G234A
-
loop mutant, weaker binding affinities to all tested ligands
G234D
-
loop mutant, weaker binding affinities to all tested ligands
G234N
-
loop mutant, weaker binding affinities to all tested ligands
G234S
-
loop mutant, higher binding affinities for 4-methylumbelliferyl beta-cellotrioside and cellotriose
G234S/G284P
-
loop mutant, higher binding affinity for 4-methylumbelliferyl beta-cellotrioside
G284A
-
loop mutant, weaker binding affinities to all tested ligands
G284I
-
loop mutant, weaker binding affinities to all tested ligands
G284P
-
loop mutant, weaker binding affinities to all tested ligands
G284P/A229V
-
loop mutant, weaker binding affinities to all tested ligands
G331A
-
active site mutant, weaker binding affinities to all tested ligands
G496P
-
loop mutant, weaker binding affinities to all tested ligands
H326A
-
active site mutant, weaker binding affinities to all tested ligands
H326S
-
active site mutant, higher binding affinities for 4-methylumbelliferyl beta-cellobioside and cellotriose
L230C
-
loop mutant, weaker binding affinities to all tested ligands
L230C/D512C
-
loop mutant, weaker binding affinities to all tested ligands
N233C
-
loop mutant, weaker binding affinities to all tested ligands
N233C/D506C
-
loop mutant, weaker binding affinities to all tested ligands
W329C
-
active site mutant, weaker binding affinities to all tested ligands
W332A
-
active site mutant, weaker binding affinities to all tested ligands
W332Y
-
active site mutant, weaker binding affinities to all tested ligands
Renatured/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
denaturation studies using GdnCl indicate that TSC folds through a partially folded state that resembles molten globule at 1.8 M GdnCl. alpha-Crystallin chaperone-mediated in vitro folding, molecular mechanism, overview. Reconstitution of the active TSC is observed in 50 mM sodium phosphate buffer, pH 7.0, on cooling the alpha-crystallin-TSC-m complex to 4C. Addition of alpha-crystallin to the molten globule-like intermediate of TSC complex initiates the refolding of TSC with 69% recovery of the biological activity of the enzyme
Thermomonospora sp.
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
-
tandem mass spectrometry to compare gas-phase and solution binding of small-molecule inhibitors to the wild type and three mutant forms of Cex
biotechnology
-
hydrolysis of lignocellulosic materials
energy production
additional information
-
obtaining of renewable energy