Information on EC 3.2.1.74 - glucan 1,4-beta-glucosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.74
-
RECOMMENDED NAME
GeneOntology No.
glucan 1,4-beta-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->4)-linkages in (1->4)-beta-D-glucans, to remove successive glucose units
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
4-beta-D-glucan glucohydrolase
Acts on 1,4-beta-D-glucans and related oligosaccharides. Cellobiose is hydrolysed, but very slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
37288-52-1
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
animal
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
strain 155 v cellulolytic fungus
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
ATCC35296
Uniprot
Manually annotated by BRENDA team
Clostridium stercorarium
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
cress
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Gilmaniella humicola
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
BPR2001
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Manually annotated by BRENDA team
BPR2001
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Myrothecium striatisporum
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
AR67
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Sporotrichum pulverulentum
also called Chrysosporium lignorum
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
Thermomonospora sp.
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
QM 9414
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Manually annotated by BRENDA team
ZU-02
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Manually annotated by BRENDA team
strain E1 Tor N16961
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Manually annotated by BRENDA team
Vibrio cholerae E1 Tor N16961
strain E1 Tor N16961
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Manually annotated by BRENDA team
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Automatic Mining of ENzyme DAta
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Automatic Mining of ENzyme DAta
yeasts
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Automatic Mining of ENzyme DAta
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenol cellobiose + H2O
?
show the reaction diagram
-
-
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?
2-nitrophenyl beta-cellobioside + H2O
2-nitrophenol + beta-cellobiose
show the reaction diagram
-
3.1% activity compared to 4-nitrophenyl beta-D-glucopyranoside
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-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
show the reaction diagram
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
show the reaction diagram
4-methylumbelliferryl 4-O-(beta-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
?
show the reaction diagram
-
-
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?
4-methylumbelliferryl-beta-cellobioside + H2O
?
show the reaction diagram
4-methylumbelliferryl-beta-cellotrioside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-D-cellotrioside + H2O
?
show the reaction diagram
-
-
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?
4-methylumbelliferryl-beta-D-glucopyranoside + H2O
?
show the reaction diagram
-
-
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?
4-methylumbelliferryl-beta-D-glucoside + H2O
?
show the reaction diagram
4-methylumbelliferyl beta-D-cellobioside + H2O
D-glucose + ?
show the reaction diagram
i.e.MuC, 14.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate
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?
4-methylumbelliferyl-beta-D-cellobioside + H2O
?
show the reaction diagram
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?
4-methylumbelliferyl-beta-D-cellobioside + H2O
D-glucose + ?
show the reaction diagram
i.e.MuC, 157.7% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate
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?
4-methylumbelliferyl-beta-D-lactoside + H2O
4-methylumbelliferone + D-lactose
show the reaction diagram
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?
4-nitrophenyl beta-cellobioside + H2O
4-nitrophenol + beta-cellobiose
show the reaction diagram
-
6.5% activity compared to 4-nitrophenyl beta-D-glucopyranoside
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?
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
show the reaction diagram
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exo-beta-1,4-glucanase activity, 24.1% activity compared to carboxymethylcellulose
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?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-
1.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside
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?
4-nitrophenyl beta-D-glucopyranose + H2O
4-nitrophenol + D-glucose
show the reaction diagram
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?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
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?
acid swollen cellulose + H2O
D-glucose + ?
show the reaction diagram
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?
acid-swollen cellulose + H2O
cellobiose
show the reaction diagram
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?
alpha-cellulose + H2O
?
show the reaction diagram
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no activity with carboxymethylcellulose, salicin, cellobiose and alpha-dimethylglucoside
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?
amygdalin + H2O
?
show the reaction diagram
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?
arbutin + H2O
?
show the reaction diagram
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?
avicel + H2O
?
show the reaction diagram
avicel + H2O
cellobiose
show the reaction diagram
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?
avicel + H2O
cellobiose + ?
show the reaction diagram
-
0.09% activity compared to carboxymethylcellulose
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?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
show the reaction diagram
bacterial cellulose + H2O
?
show the reaction diagram
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?
bacterial microcrystalline cellulose + H2O
?
show the reaction diagram
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?
barley glucan + H2O
?
show the reaction diagram
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326% activity compared to carboxymethylcellulose
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?
beta-1,4-xylan + H2O
?
show the reaction diagram
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?
beta-glucan + H2O
?
show the reaction diagram
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from yeast and from barley
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?
carboxymethyl cellulose + H2O
?
show the reaction diagram
carboxymethyl cellulose + H2O
cellobiose + cellopentaose + cellotetraose + cellotriose + D-glucose
show the reaction diagram
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the major product is cellobiose with some minor products
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?
carboxymethylcellulose + H2O
?
show the reaction diagram
carboxymethylcellulose + H2O
D-glucose + cellooligosaccharides
show the reaction diagram
Thermomonospora sp.
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?
cellobiose + H2O
2 beta-D-glucose
show the reaction diagram
cellobiose + H2O
2 D-glucose
show the reaction diagram
cellobiose + H2O
D-glucose
show the reaction diagram
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
cellodextrins + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
celloheptaose + H2O
?
show the reaction diagram
cellohexaose + H2O
cellopentaose + D-glucose
show the reaction diagram
-
-
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?
cellohexaose + H2O
D-glucose
show the reaction diagram
cellooligomers + H2O
cellobiose + cellooligomers
show the reaction diagram
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ir
cellooligomers + H2O
D-glucose
show the reaction diagram
cellooligomers + H2O
D-glucose + cellooligomers
show the reaction diagram
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ir
cellopentaose + H2O
?
show the reaction diagram
cellopentaose + H2O
D-glucose
show the reaction diagram
cellopentaose + H2O
glucose
show the reaction diagram
cellotetraose + 3 H2O
4 D-glucose
show the reaction diagram
cellotetraose + H2O
?
show the reaction diagram
cellotetraose + H2O
cellobiose + cellotriose + D-glucose
show the reaction diagram
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cellobiose is the dominant product, while cellotriose and glucose are minor products
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
cellotetraose + H2O
glucose + cellobiose + cellotriose
show the reaction diagram
-
with a conversion ratio of 80%
-
?
cellotriose + H2O
D-glucose
show the reaction diagram
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
cellotriose + H2O
glucose
show the reaction diagram
cellulose + H2O
?
show the reaction diagram
cellulose + H2O
D-glucose
show the reaction diagram
cellulose + H2O
D-glucose + ?
show the reaction diagram
cellulose acetate + H2O
?
show the reaction diagram
-
-
-
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?
cotton + H2O
?
show the reaction diagram
-
-
-
-
?
D(+)-cellobiose + H2O
D-glucose
show the reaction diagram
-
-
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-
r
esculin + H2O
?
show the reaction diagram
-
-
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?
filter paper
?
show the reaction diagram
-
-
-
?
filter paper + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
filter paper + H2O
cellobiose + ?
show the reaction diagram
-
0.02% activity compared to carboxymethylcellulose
-
-
?
filter paper + H2O
cellobiose + cellopentaose + cellotetraose + cellotriose + D-glucose
show the reaction diagram
-
-
the major product is cellobiose with some minor products
-
?
gentiobiose + H2O
?
show the reaction diagram
hydroquinone-beta-D-glucopyranoside + H2O
hydroquinone + D-glucose
show the reaction diagram
-
-
-
?
laminaribiose + H2O
?
show the reaction diagram
laminarin + H2O
?
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
?
show the reaction diagram
-
from barley
-
-
?
lichenan + H2O
?
show the reaction diagram
n-heptyl-beta-D-glucopyranoside + H2O
n-heptanol + D-glucose
show the reaction diagram
-
-
-
?
n-hexyl-beta-D-glucopyranoside + H2O
n-hexanol + D-glucose
show the reaction diagram
-
-
-
?
n-octyl-beta-D-glucopyranoside + H2O
n-octanol + D-glucose
show the reaction diagram
-
-
-
?
n-pentyl-beta-D-glucopyranoside + H2O
n-pentanol + D-glucose
show the reaction diagram
-
-
-
?
o-nitrophenyl beta-D-glucopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranose + H2O
o-nitrophenol + beta-D-galactopyranose
show the reaction diagram
p-nitrophenol beta-D-cellobioside + H2O
p-nitrophenol beta-D-glucopyranoside + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl beta-D-lactoside + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl cellobioside + H2O
p-nitrophenyl + glucose
show the reaction diagram
p-nitrophenyl glucoside + H2O
p-nitrophenyl + glucose
show the reaction diagram
-
p-nitrophenyl releasing with no lag period, no activity with p-nitrophenyl-?-(N-acetylgalactosamine), p-nitrophenyl-?-glucose-6-phosphate, p-nitrophenyl-(N-acetylglucosamine), p-nitrophenyl-di(N-acetylglucosamine), p-nitrophenyl-tri(N-acetylglucosamine)
-
-
?
p-nitrophenyl-beta-D-cellobiose + H2O
p-nitrophenol + cellobioside
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
cellobiose + p-nitrophenol
show the reaction diagram
-
-
-
ir
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
p-nitrophenyl-beta-D-cellotrioside + H2O
D-glucose + p-nitrophenyl-beta-D-cellobioside + p-nitrophenyl-beta-D-cellotetraoside + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
p-nitrophenyl-beta-D-fucopyranoside + H2O
p-nitrophenol + fucopyranose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
p-nitrophenyl-cellobioside + H2O
?
show the reaction diagram
-
-
-
-
?
phenyl-beta-D-glucopyranoside + H2O
phenol + D-glucose
show the reaction diagram
-
-
-
?
phosphate acid swollen cellulose + H2O
?
show the reaction diagram
-
10.7% activity compared to carboxymethylcellulose
-
-
?
prunasin + H2O
D-mandelonitrile + beta-D-glucose
show the reaction diagram
-
-
-
-
?
salicin + H2O
?
show the reaction diagram
sigmacell + H2O
?
show the reaction diagram
-
-
-
-
?
sophorose + H2O
?
show the reaction diagram
sugarcane bagasse + H2O
?
show the reaction diagram
swollen cellulose + H2O
cellobiose + cellotriose
show the reaction diagram
-
-
-
?
trehalose + H2O
?
show the reaction diagram
xylan + H2O
?
show the reaction diagram
xylo-oligosaccharides + H2O
?
show the reaction diagram
-
-
-
-
?
xylobiose + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cellobiose + H2O
D-glucose
show the reaction diagram
-
hydrolysis of cellulose
-
-
ir
cellulose + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
5 mM, 107.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 92.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Co2+
-
5 min at 50C, 10 mM, 101.9% of the relative activity without metal ions; 5 min at 50C, 1 mM, 103.0% of the relative activity without metal ions
CoCl2
5 mM, 79.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 85.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
CuCl2
5 mM, 79.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 83.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-fructose
5 mM, 102.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-glucose
5 mM,82% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-xylose
5 mM, 106.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Fe3+
-
5 min at 50C, 10 mM, 103.6% of the relative activity without metal ions; 5 min at 50C, 1 mM, 105.5% of the relative activity without metal ions
fructose
5 mM, 107.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
glucose
5 mM,24.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
MgCl2
5 mM, 110.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 96.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Mn2+
-
5 min at 50C, 10 mM, 104.1% of the relative activity without metal ions; 5 min at 50C, 1 mM, 105.8% of the relative activity without metal ions
NiCl2
5 mM, 76.7% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 86.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
xylose
5 mM, 88% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
ZnSO4
5 mM, 111.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 90.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-anhydro-myo-inositol
-
-
Ag+
-
5 min at 50C, 10mM, 100.0% of inhibition; 5 min at 50C, 1 mM, 100.0% inhibition
D-cellobiose
D-glucono-1,5-lactone
-
-
D-glucose
EGTA
-
10% of enzyme inhibition at 20 mM, at 37C, pH 6.5
Fe3+
-
80.5% residual activity at 10 mM
iodoacetoamide
KCl
-
25% of enzyme inhibition at 0.5 mM, at 37C, pH 6.5
Mg2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Mg2+
N-bromosuccinimide
Ni2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ni2+
o-phthaldialdehyde
Thermomonospora sp.
-
-
p-chloromercuribenzoate
-
-
xylobiosyl-deoxynojirimycin
-
X2DNJ
xylobiosyl-isofagomine
-
X2IF
xylobiosyl-isofagomine lactam
-
X2IL
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ethanol
methanol
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.74
4-nitrophenyl beta-D-cellobioside
-
at 65C in pH 5.5 citrate buffer
0.22 - 2.5
4-nitrophenyl beta-D-glucopyranoside
0.65
alpha-cellulose
-
pH 5.0
-
0.461 - 86
cellobiose
0.028 - 0.73
cellohexaose
0.37 - 6
Cellopentaose
0.77 - 6.7
Cellotetraose
0.44 - 8.7
cellotriose
127
cellulose
-
H3OP4 swollen
10.7
gentiobiose
-
-
0.77
o-nitrophenyl beta-D-glucopyranoside
-
-
0.45
p-nitrophenyl glucoside
-
pH 6.5, at 37C
0.7
p-nitrophenyl-beta-D-cellobioside
2.8 - 4
p-nitrophenyl-beta-D-glucopyranoside
0.42 - 2.2
p-nitrophenyl-beta-D-glucoside
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00039
2,4-dinitrophenol cellobiose
Thermobifida fusca
Q9XCD4
pH 5.5
69.6
4-nitrophenyl beta-D-cellobioside
uncultured bacterium
-
at 65C in pH 5.5 citrate buffer
126.6 - 4945
4-nitrophenyl beta-D-glucopyranoside
0.25 - 281
cellobiose
16.3
cellohexaose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
16.4
Cellopentaose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
18.6
Cellotetraose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
6.21
cellotriose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
40.1
4-nitrophenyl beta-D-cellobioside
uncultured bacterium
-
at 65C in pH 5.5 citrate buffer
4692
11900 - 915700
4-nitrophenyl beta-D-glucopyranoside
510
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
29
D-glucose
-
in 100 mM sodium acetate buffer (pH 4.5), at 65C
0.0058 - 0.18
xylobiosyl-deoxynojirimycin
0.00013 - 0.0071
xylobiosyl-isofagomine
0.00034 - 0.024
xylobiosyl-isofagomine lactam
SPECIFIC ACTIVITY [µmol/min/mg]