Information on EC 3.2.1.74 - glucan 1,4-beta-glucosidase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Mark a special word or phrase in this record:
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.2.1.74
-
RECOMMENDED NAME
GeneOntology No.
glucan 1,4-beta-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Hydrolysis of (1->4)-linkages in (1->4)-beta-D-glucans, to remove successive glucose units
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
hydrolysis of O-glycosyl bond
Q53EH2
-
hydrolysis of O-glycosyl bond
-
-
hydrolysis of O-glycosyl bond
-
-
hydrolysis of O-glycosyl bond
Clostridium cellulovorans ATCC35296, Hypocrea jecorina ZU-02
-
-
-
PATHWAY
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
-
SYSTEMATIC NAME
IUBMB Comments
4-beta-D-glucan glucohydrolase
Acts on 1,4-beta-D-glucans and related oligosaccharides. Cellobiose is hydrolysed, but very slowly.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
1,4-beta-D-glucan glucanohydrolase
-
-
1,4-beta-D-glucan glucanohydrolase
Thermomonospora sp.
-
-
1,4-beta-D-glucan glucohydrolase
B0FWI3
-
b-glucan glucohydrolase
Q53EH2
extracellular non-cellulosomal beta-glucan glucohydrolase
b-glucan glucohydrolase
Clostridium cellulovorans ATCC35296
Q53EH2
extracellular non-cellulosomal beta-glucan glucohydrolase
-
beta-1,4-beta-glucanase
-
-
-
-
beta-1,4-glucosidase
-
-
beta-1,4-glucosidase
-
-
beta-1,4-glucosidase
Stereum hirsutum SKU512
-
-
-
beta-1,4-glucosidase
-
-
beta-1,4-glucosidase
Vibrio cholerae E1 Tor N16961
-
-
-
beta-glucosidase
-
-
-
-
beta-glucosidase
G3BJX3, G3BJX4
-
beta-glucosidase
-
-
beta-glucosidase
Vibrio cholerae E1 Tor N16961
-
-
-
beta-glucosidase/xylosidase
G3BJX3, G3BJX4
bifunctional, belongs to to the glycoside hydrolase 3 familiy, GH3
Bgl
Stereum hirsutum SKU512
-
-
-
BglA
Clostridium cellulovorans ATCC35296
Q53EH2
-
-
BglA
Vibrio cholerae E1 Tor N16961
-
-
-
Carboxymethyl cellulase
Thermomonospora sp.
-
-
Cel48A
Q9XCD4
-
cellobiase
-
-
cellobiase
-
-
cellobiase
Vibrio cholerae E1 Tor N16961
-
-
-
cellobiohydrolase
-
-
Cellulase
-
-
Cellulase
Hypocrea jecorina ZU-02
-
-
-
endo/exocellulase
-
-
endo/exocellulase
-
-
Ex-1
-
native enzyme
exo-1,4-beta-glucanase
-
-
-
-
exo-1,4-beta-glucanase
-
-
exo-1,4-beta-glucosidase
-
-
-
-
exo-beta-1,4-glucanase
-
-
-
-
exo-beta-1,4-glucanase
-
-
exo-beta-1,4-glucanase
Aspergillus fumigatus 155
-
-
-
exo-beta-1,4-glucosidase
-
-
-
-
exo-bets-1,4-glucanase
-
-
exocellulase
-
-
-
-
exocellulase
-
-
exocellulase
Q9XCD4
-
exocellulase I
-
-
exoglucanase
-
-
family 9 glycoside hydrolase
B0FWI3
-
family-1 glycosyl hydrolase
Q53EH2
-
family-1 glycosyl hydrolase
Clostridium cellulovorans ATCC35296
Q53EH2
-
-
glucan 1,4-beta-glucosidase
-
-
glucan 1,4-beta-glucosidase
Q53EH2
-
glucan 1,4-beta-glucosidase
Clostridium cellulovorans ATCC35296
Q53EH2
-
-
glucan 1,4-beta-glucosidase
-
-
glucan 1,4-beta-glucosidase
Hypocrea jecorina ZU-02
-
-
-
glucohydrolase
-
-
P-42
-
core fragment
Rubg3A
G3BJX3
gene name, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74
Rubg3B
G3BJX4
gene name, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74
TSC
Thermomonospora sp.
-
-
CAS REGISTRY NUMBER
COMMENTARY
37288-52-1
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain 155 v cellulolytic fungus
-
-
Manually annotated by BRENDA team
Aspergillus fumigatus 155
strain 155 v cellulolytic fungus
-
-
Manually annotated by BRENDA team
Clostridium cellulovorans ATCC35296
ATCC35296
Uniprot
Manually annotated by BRENDA team
Geomyces pannorum
-
-
-
Manually annotated by BRENDA team
Gilmaniella humicola
-
-
-
Manually annotated by BRENDA team
Gluconacetobacter xylinus
BPR2001
-
-
Manually annotated by BRENDA team
Gluconacetobacter xylinus BPR2001
BPR2001
-
-
Manually annotated by BRENDA team
Hypocrea jecorina QM 9414
QM 9414
-
-
Manually annotated by BRENDA team
Hypocrea jecorina ZU-02
ZU-02
-
-
Manually annotated by BRENDA team
Myrothecium striatisporum
-
-
-
Manually annotated by BRENDA team
Penicillium funiculosum
-
-
-
Manually annotated by BRENDA team
Phoma sp.
-
-
-
Manually annotated by BRENDA team
subsp. cellulosa
-
-
Manually annotated by BRENDA team
Ruminococcus albus AR67
AR67
-
-
Manually annotated by BRENDA team
Sporotrichum pulverulentum
also called Chrysosporium lignorum
-
-
Manually annotated by BRENDA team
Stereum hirsutum SKU512
-
-
-
Manually annotated by BRENDA team
Thermomonospora sp.
-
-
-
Manually annotated by BRENDA team
from yak rumen
-
-
Manually annotated by BRENDA team
Rubg3A, together with Rubg3B, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
UniProt
Manually annotated by BRENDA team
Rubg3B, together with Rubg3A, beta-glucosidase/xylosidase activity, EC 3.2.1.37 and 3.2.1.74; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
UniProt
Manually annotated by BRENDA team
strain E1 Tor N16961
-
-
Manually annotated by BRENDA team
Vibrio cholerae E1 Tor N16961
strain E1 Tor N16961
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenol cellobiose + H2O
?
show the reaction diagram
Q9XCD4, -
-
-
-
?
2-nitrophenyl beta-cellobioside + H2O
2-nitrophenol + beta-cellobiose
show the reaction diagram
-
3.1% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
show the reaction diagram
G3BJX3, G3BJX4
0.8% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
show the reaction diagram
G3BJX3, G3BJX4
1.3% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucopyranose
show the reaction diagram
Stereum hirsutum, Stereum hirsutum SKU512
-
25.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-methylumbelliferryl 4-O-(beta-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-cellobioside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-cellobioside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-cellotrioside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-D-cellotrioside + H2O
?
show the reaction diagram
Q9XCD4, -
-
-
-
?
4-methylumbelliferryl-beta-D-glucopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-D-glucoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-D-glucoside + H2O
?
show the reaction diagram
Gluconacetobacter xylinus, Gluconacetobacter xylinus BPR2001
-
-
-
-
?
4-methylumbelliferyl beta-D-cellobioside + H2O
D-glucose + ?
show the reaction diagram
G3BJX3, G3BJX4
i.e.MuC, 14.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate
-
-
?
4-methylumbelliferyl-beta-D-cellobioside + H2O
?
show the reaction diagram
Q9XCD4, -
-
-
-
?
4-methylumbelliferyl-beta-D-cellobioside + H2O
D-glucose + ?
show the reaction diagram
G3BJX3, G3BJX4
i.e.MuC, 157.7% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate
-
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
4-methylumbelliferone + D-lactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-cellobioside + H2O
4-nitrophenol + beta-cellobiose
show the reaction diagram
-
6.5% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
show the reaction diagram
-
exo-beta-1,4-glucanase activity, 24.1% activity compared to carboxymethylcellulose
-
-
?
4-nitrophenyl beta-D-glucopyranose + H2O
4-nitrophenol + D-glucose
show the reaction diagram
Q53EH2
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucopyranose
show the reaction diagram
Stereum hirsutum, Stereum hirsutum SKU512
-
100% activity
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
G3BJX3, G3BJX4
-
-
-
?
4-nitrophenyl beta-galactopyranoside + H2O
4-nitrophenol + beta-galactopyranose
show the reaction diagram
-
1.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
acid swollen cellulose + H2O
D-glucose + ?
show the reaction diagram
B0FWI3
-
-
-
?
acid-swollen cellulose + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
alpha-cellulose + H2O
?
show the reaction diagram
-
no activity with carboxymethylcellulose, salicin, cellobiose and alpha-dimethylglucoside
-
-
?
amorphous cellulose + H2O
?
show the reaction diagram
B0FWI3
-
-
-
?
amygdalin + H2O
?
show the reaction diagram
-
-
-
-
?
arbutin + H2O
?
show the reaction diagram
-
-
-
-
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
show the reaction diagram
-
-
-
-
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
show the reaction diagram
-
-
-
-
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
show the reaction diagram
Sporotrichum pulverulentum
-
-
-
-
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
show the reaction diagram
-
-
-
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
show the reaction diagram
Penicillium funiculosum
-
-
D-glucose only
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
show the reaction diagram
-
H3PO4 or HCl swollen
cellobiose and cellotriose only
?
avicel + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
avicel + H2O
cellobiose + ?
show the reaction diagram
-
0.09% activity compared to carboxymethylcellulose
-
-
?
bacterial cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
bacterial microcrystalline cellulose + H2O
?
show the reaction diagram
Q9XCD4, -
-
-
-
?
barley glucan + H2O
?
show the reaction diagram
-
326% activity compared to carboxymethylcellulose
-
-
?
beta-1,4-xylan + H2O
?
show the reaction diagram
-
-
-
-
?
beta-glucan + H2O
?
show the reaction diagram
-
from yeast and from barley
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
Q9XCD4, -
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
B0FWI3
-
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
carboxymethyl cellulose + H2O
cellobiose + cellopentaose + cellotetraose + cellotriose + D-glucose
show the reaction diagram
-
-
the major product is cellobiose with some minor products
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
Thermomonospora sp.
-
-
-
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
-
endo-beta-1,4-glucanase activity, 100% activity
-
-
?
carboxymethylcellulose + H2O
D-glucose + cellooligosaccharides
show the reaction diagram
Thermomonospora sp.
-
-
-
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
Q53EH2
-
-
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
-
hydrolysis of cellulose
-
-
ir
cellobiose + H2O
D-glucose
show the reaction diagram
B0FWI3
is degraded very slowly
-
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
G3BJX3, G3BJX4
128.8% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate
cellobiose is completely hydrolyzed to glucose, evaluated by chromatographic analysis
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
G3BJX3, G3BJX4
6.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate
31.5% cellobiose is hydrolyzed to glucose, evaluated by chromatographic analysis
-
?
cellobiose + H2O
D-glucose
show the reaction diagram
Clostridium cellulovorans ATCC35296
Q53EH2
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
-
-
-
-
ir
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
Gluconacetobacter xylinus
-
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
Penicillium funiculosum
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
-
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
-
-
D-glucose only
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
-
only isoenzyme I
-
-
-
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
-
glucosyl transfer reaction
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
Ruminococcus albus AR67
-
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
Hypocrea jecorina QM 9414
-
-
-
-
?
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
Gluconacetobacter xylinus BPR2001
-
-
-
-
?
cellobiose + H2O
2 beta-D-glucose
show the reaction diagram
Stereum hirsutum, Stereum hirsutum SKU512
-
0.96% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
cellobiose + H2O
2 D-glucose
show the reaction diagram
Vibrio cholerae, Vibrio cholerae E1 Tor N16961
-
no activity with di(N-acetylglucosamine), chitobiose, chitin oligosaccharides, trehalose, lactose, maltose, sucrose, sophorose laminaribiose and gentiobiose
-
-
?
cellodextrins + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
-
?
cellodextrins + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
-
?
cellodextrins + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
D-glucose only
?
cellodextrins + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
at high substrate concentrations also transglycosylation reactions
?
celloheptaose + H2O
?
show the reaction diagram
-
-
-
-
?
celloheptaose + H2O
?
show the reaction diagram
-
-
-
-
?
cellohexaose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
cellohexaose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
cellohexaose + H2O
D-glucose
show the reaction diagram
-
-
-
?
cellohexaose + H2O
D-glucose
show the reaction diagram
-
-
in the early stages also cellopentaose and cellotetraose
?
cellohexaose + H2O
D-glucose
show the reaction diagram
-
-
cellotetraose, cellotriose and cellobiose only
?
cellohexaose + H2O
D-glucose
show the reaction diagram
Hypocrea jecorina QM 9414
-
-
-
-
?
cellohexaose + H2O
cellopentaose + D-glucose
show the reaction diagram
B0FWI3
-
-
-
?
cellooligomers + H2O
D-glucose + cellooligomers
show the reaction diagram
-
-
-
ir
cellooligomers + H2O
cellobiose + cellooligomers
show the reaction diagram
-
-
-
ir
cellooligomers + H2O
D-glucose
show the reaction diagram
Clostridium cellulovorans, Clostridium cellulovorans ATCC35296
Q53EH2
-
-
-
?
cellopentaose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
D-glucose
show the reaction diagram
-
-
-
?
cellopentaose + H2O
D-glucose
show the reaction diagram
-
-
-
-
-
cellopentaose + H2O
D-glucose
show the reaction diagram
-
-
-
-
ir
cellopentaose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
D-glucose
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
cellopentaose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
D-glucose
show the reaction diagram
-
-
-
-
?
cellopentaose + H2O
D-glucose
show the reaction diagram
-
-
cellobiose and cellotriose only
?
cellopentaose + H2O
D-glucose
show the reaction diagram
Hypocrea jecorina QM 9414
-
-
-
-
?
cellopentaose + H2O
?
show the reaction diagram
Q9XCD4, -
-
-
-
?
cellopentaose + H2O
?
show the reaction diagram
B0FWI3
-
-
-
?
cellopentaose + H2O
glucose
show the reaction diagram
Vibrio cholerae, Vibrio cholerae E1 Tor N16961
-
-
-
-
?
cellotetraose + 3 H2O
4 D-glucose
show the reaction diagram
-
-
-
-
?
cellotetraose + 3 H2O
4 D-glucose
show the reaction diagram
Q53EH2
-
-
-
?
cellotetraose + 3 H2O
4 D-glucose
show the reaction diagram
Vibrio cholerae E1 Tor N16961
-
-
-
-
?
cellotetraose + 3 H2O
4 D-glucose
show the reaction diagram
Clostridium cellulovorans ATCC35296
Q53EH2
-
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
-
-
ir
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
-
cellobiose only
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
-
highest substrate preference for cellotriose and cellotetraose
-
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
Hypocrea jecorina QM 9414
-
-
-
-
?
cellotetraose + H2O
?
show the reaction diagram
B0FWI3
-
-
-
?
cellotetraose + H2O
?
show the reaction diagram
Q9XCD4, -
no activity with cellotriose and chitin
-
-
?
cellotetraose + H2O
cellobiose + cellotriose + D-glucose
show the reaction diagram
-
-
cellobiose is the dominant product, while cellotriose and glucose are minor products
-
?
cellotetraose + H2O
glucose + cellobiose + cellotriose
show the reaction diagram
G3BJX3, G3BJX4
-
with a conversion ratio of 80%
-
?
cellotriose + H2O
?
show the reaction diagram
B0FWI3
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
-
-
-
-
ir
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
-
-
-
-
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
-
-
D-glucose only
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
-
highest substrate preference for cellotriose and cellotetraose
-
-
?
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
Hypocrea jecorina QM 9414
-
-
-
-
?
cellotriose + H2O
glucose
show the reaction diagram
Vibrio cholerae, Vibrio cholerae E1 Tor N16961
-
-
-
-
?
cellotriose + H2O
D-glucose
show the reaction diagram
Clostridium cellulovorans, Clostridium cellulovorans ATCC35296
Q53EH2
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
cellulose + H2O
?
show the reaction diagram
-
nutritional resource
-
-
?
cellulose + H2O
?
show the reaction diagram
-
principal role in cellulolysis
-
-
?
cellulose + H2O
D-glucose
show the reaction diagram
-
ball-milled
-
-
?
cellulose + H2O
D-glucose
show the reaction diagram
Penicillium funiculosum
-
Walseth cellulose
-
?
cellulose + H2O
D-glucose
show the reaction diagram
-
H3PO4 swollen
-
-
?
cellulose + H2O
D-glucose
show the reaction diagram
Penicillium funiculosum
-
H3PO4 swollen
D-glucose + cellobiose
?
cellulose + H2O
D-glucose
show the reaction diagram
-
bacterial microcrystalline cellulose
-
-
?
cellulose + H2O
D-glucose
show the reaction diagram
-
cellulose HBS and cellulose MN300
-
-
?
cellulose + H2O
D-glucose
show the reaction diagram
-
bacterial cellulose powder, not dried
-
-
?
cellulose + H2O
D-glucose
show the reaction diagram
Gluconacetobacter xylinus
-
H3PO4 swollen and regenerated
-
-
?
cellulose + H2O
D-glucose + ?
show the reaction diagram
-
hydrolysis of natural corncob cellulosic residues
-
-
?
cellulose + H2O
D-glucose + ?
show the reaction diagram
-
the glucohydrolase attaches directly to crystalline cellulose to release D-glucose from free chain ends
-
-
?
cellulose + H2O
D-glucose + ?
show the reaction diagram
Hypocrea jecorina ZU-02
-
hydrolysis of natural corncob cellulosic residues
-
-
?
cellulose acetate + H2O
?
show the reaction diagram
-
-
-
-
?
cotton + H2O
?
show the reaction diagram
-
-
-
-
?
D(+)-cellobiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
r
esculin + H2O
?
show the reaction diagram
-
-
-
-
?
filter paper
?
show the reaction diagram
Q9XCD4, -
-
-
-
?
filter paper + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
filter paper + H2O
cellobiose + ?
show the reaction diagram
-
0.02% activity compared to carboxymethylcellulose
-
-
?
filter paper + H2O
cellobiose + cellopentaose + cellotetraose + cellotriose + D-glucose
show the reaction diagram
-
-
the major product is cellobiose with some minor products
-
?
gentiobiose + H2O
?
show the reaction diagram
-
-
-
-
?
gentiobiose + H2O
?
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
gentiobiose + H2O
?
show the reaction diagram
-
-
-
-
?
gentiobiose + H2O
?
show the reaction diagram
-
-
-
-
?
hydroquinone-beta-D-glucopyranoside + H2O
hydroquinone + D-glucose
show the reaction diagram
-
-
-
?
laminaribiose + H2O
?
show the reaction diagram
-
-
-
-
?
laminaribiose + H2O
?
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
laminaribiose + H2O
?
show the reaction diagram
-
-
-
-
?
laminaribiose + H2O
?
show the reaction diagram
Ruminococcus albus AR67
-
-
-
-
?
laminarin + H2O
?
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
?
show the reaction diagram
-
from barley
-
-
?
lichenan + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
Gluconacetobacter xylinus
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
-
315% activity compared to carboxymethylcellulose
-
-
?
lichenan + H2O
?
show the reaction diagram
Ruminococcus albus AR67
-
-
-
-
?
n-heptyl-beta-D-glucopyranoside + H2O
n-heptanol + D-glucose
show the reaction diagram
-
-
-
?
n-hexyl-beta-D-glucopyranoside + H2O
n-hexanol + D-glucose
show the reaction diagram
-
-
-
?
n-octyl-beta-D-glucopyranoside + H2O
n-octanol + D-glucose
show the reaction diagram
-
-
-
?
n-pentyl-beta-D-glucopyranoside + H2O
n-pentanol + D-glucose
show the reaction diagram
-
-
-
?
o-nitrophenyl beta-D-glucopyranoside + H2O
?
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranose + H2O
o-nitrophenol + beta-D-galactopyranose
show the reaction diagram
Clostridium cellulovorans, Clostridium cellulovorans ATCC35296
Q53EH2
-
-
-
?
p-nitrophenol beta-D-cellobioside + H2O
p-nitrophenol beta-D-glucopyranoside + D-glucose
show the reaction diagram
B0FWI3
-
-
-
?
p-nitrophenyl beta-D-lactoside + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl cellobioside + H2O
p-nitrophenyl + glucose
show the reaction diagram
Vibrio cholerae, Vibrio cholerae E1 Tor N16961
-
p-nitrophenyl releasing after a lag period
-
-
?
p-nitrophenyl glucoside + H2O
p-nitrophenyl + glucose
show the reaction diagram
-
p-nitrophenyl releasing with no lag period, no activity with p-nitrophenyl-?-(N-acetylgalactosamine), p-nitrophenyl-?-glucose-6-phosphate, p-nitrophenyl-(N-acetylglucosamine), p-nitrophenyl-di(N-acetylglucosamine), p-nitrophenyl-tri(N-acetylglucosamine)
-
-
?
p-nitrophenyl-beta-D-cellobiose + H2O
p-nitrophenol + cellobioside
show the reaction diagram
Q53EH2
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
cellobiose + p-nitrophenol
show the reaction diagram
-
-
-
ir
p-nitrophenyl-beta-D-cellotrioside + H2O
D-glucose + p-nitrophenyl-beta-D-cellobioside + p-nitrophenyl-beta-D-cellotetraoside + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
Gluconacetobacter xylinus, Gluconacetobacter xylinus BPR2001
-
-
-
?
p-nitrophenyl-beta-D-fucopyranoside + H2O
p-nitrophenol + fucopyranose
show the reaction diagram
Q53EH2
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
Gluconacetobacter xylinus
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
when incubated with 100 mM substrate, products are also 4-nitrophenyl-beta-laminaribioside, 4-nitrophenyl-beta-cellobioside and 4-nitrophenyl-beta-gentiobioside
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
Gluconacetobacter xylinus BPR2001
-
-
-
?
p-nitrophenyl-cellobioside + H2O
?
show the reaction diagram
-
-
-
-
?
phenyl-beta-D-glucopyranoside + H2O
phenol + D-glucose
show the reaction diagram
-
-
-
?
phosphate acid swollen cellulose + H2O
?
show the reaction diagram
-
10.7% activity compared to carboxymethylcellulose
-
-
?
prunasin + H2O
D-mandelonitrile + beta-D-glucose
show the reaction diagram
-
-
-
-
?
salicin + H2O
?
show the reaction diagram
Ruminococcus albus, Ruminococcus albus AR67
-
-
-
-
?
sigmacell + H2O
?
show the reaction diagram
-
-
-
-
?
sophorose + H2O
?
show the reaction diagram
Penicillium funiculosum
-
-
-
-
?
sophorose + H2O
?
show the reaction diagram
-
-
-
-
?
sugarcane bagasse + H2O
?
show the reaction diagram
Aspergillus fumigatus, Aspergillus fumigatus 155
-
-
-
-
?
swollen cellulose + H2O
cellobiose + cellotriose
show the reaction diagram
Q9XCD4, -
-
-
-
?
trehalose + H2O
?
show the reaction diagram
Ruminococcus albus, Ruminococcus albus AR67
-
slight hydrolysis
-
-
?
xylan + H2O
?
show the reaction diagram
-
-
-
-
?
xylan + H2O
?
show the reaction diagram
-
-
-
-
?
xylo-oligosaccharides + H2O
?
show the reaction diagram
-
-
-
-
?
xylobiose + H2O
?
show the reaction diagram
-
-
-
-
?
lichenan + H2O
?
show the reaction diagram
Gluconacetobacter xylinus BPR2001
-
-
-
-
?
additional information
?
-
Thermomonospora sp.
-
the enzyme exhibits both endocarboxymethyl cellulase and endoxylanase activity, both substrates are hydrolyzed at different active sites
-
-
-
additional information
?
-
B0FWI3
no activity on laminarin, sophorose, laminaribiose, gentiobiose, p-nitrophenol-alpha-L-arabinofuranoside, p-nitrophenol-alpha-D-glucoside, p-nitrophenol-beta-D-cellobioside, p-nitrophenol-beta-D-fucoside, p-nitrophenol-beta-D-galactoside, p-nitrophenol-N-acetyl-beta-D-glucosaminide, p-nitrophenol-beta-D-glucoside, p-nitrophenol-beta-D-glucuronoside, p-nitrophenol-beta-D-lactoside, or p-nitrophenol-beta-D-maltoside
-
-
-
additional information
?
-
Thermomonospora sp.
-
fluorescence study on interactions of chaperone alpha-crystallin with the molten globule state of the enzyme induced by guanidine hydrochloride
-
-
-
additional information
?
-
-
endo/exocellulase Cel9A does not fully hydrolyze the cellobiose, even with longer incubations
-
-
-
additional information
?
-
-
no activity is detected towards carboxymethyl cellulose
-
-
-
additional information
?
-
-
no activity with cellotriose, cellotetraose, cellopentaose, carboxy-methylcellulose, xylan, laminarin, avicel (2% w/v), 4-nitrophenyl beta-D-mannopyranoside, and 4-nitrophenyl alpha-D-glucopyranoside
-
-
-
additional information
?
-
-
no activity with laminarin, glucan, birchwood xylan, and 4-nitrophenyl beta-D-glucopyranside, the cellulase Rucel5B possesses endo-beta-1,4-glucanase activity and exo-beta-1,4-glucanase activity
-
-
-
additional information
?
-
-
the enzyme is able to synthesise some cellotetraose by the transglycosylation of smaller substrates
-
-
-
additional information
?
-
-
the enzyme is also able to promote transglycosylation using methanol as an acceptor and glucose as a donor
-
-
-
additional information
?
-
G3BJX3, G3BJX4
no activity with 4-nitrophenyl alpha-D-glucopyranoside and 4-methylumbelliferyl alpha-D-galactopyranoside as substrates
-
-
-
additional information
?
-
G3BJX3, G3BJX4
no activity with 4-nitrophenyl alpha-D-glucopyranoside, cellotetraose, and 4-methylumbelliferyl alpha-D-galactopyranoside as substrates
-
-
-
additional information
?
-
Stereum hirsutum SKU512
-
no activity with cellotriose, cellotetraose, cellopentaose, carboxy-methylcellulose, xylan, laminarin, avicel (2% w/v), 4-nitrophenyl beta-D-mannopyranoside, and 4-nitrophenyl alpha-D-glucopyranoside, the enzyme is also able to promote transglycosylation using methanol as an acceptor and glucose as a donor
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cellobiose + H2O
D-glucose
show the reaction diagram
-
hydrolysis of cellulose
-
-
ir
cellulose + H2O
?
show the reaction diagram
-
nutritional resource
-
-
?
cellulose + H2O
?
show the reaction diagram
-
principal role in cellulolysis
-
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
CaCl2
G3BJX3, G3BJX4
5 mM, 107.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 92.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Co2+
-
5 min at 50C, 10 mM, 101.9% of the relative activity without metal ions; 5 min at 50C, 1 mM, 103.0% of the relative activity without metal ions
CoCl2
G3BJX3, G3BJX4
5 mM, 79.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 85.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
CuCl2
G3BJX3, G3BJX4
5 mM, 79.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 83.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-fructose
G3BJX3, G3BJX4
5 mM, 102.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-glucose
G3BJX3, G3BJX4
5 mM,82% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-xylose
G3BJX3, G3BJX4
5 mM, 106.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Fe3+
-
5 min at 50C, 10 mM, 103.6% of the relative activity without metal ions; 5 min at 50C, 1 mM, 105.5% of the relative activity without metal ions
fructose
G3BJX3, G3BJX4
5 mM, 107.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
glucose
G3BJX3, G3BJX4
5 mM,24.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
MgCl2
G3BJX3, G3BJX4
5 mM, 110.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 96.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Mn2+
-
5 min at 50C, 10 mM, 104.1% of the relative activity without metal ions; 5 min at 50C, 1 mM, 105.8% of the relative activity without metal ions
MnCl2
-
activity increased by 58%
NiCl2
G3BJX3, G3BJX4
5 mM, 76.7% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 86.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
xylose
G3BJX3, G3BJX4
5 mM, 88% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
ZnSO4
G3BJX3, G3BJX4
5 mM, 111.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 90.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
MnCl2
G3BJX3, G3BJX4
5 mM, 102.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C; 5 mM, 112% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
additional information
-
NaCl and KCl gives optimum results at 10 mM, at 37C, pH 6.5
additional information
-
divalent cations are not required for enzyme activation
additional information
-
the addition of 5 mM Zn2+, Co2+ and Cu2+ has no statistical difference on enzyme activity
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1,2-anhydro-myo-inositol
-
-
Ag+
-
5 min at 50C, 10mM, 100.0% of inhibition; 5 min at 50C, 1 mM, 100.0% inhibition
Ca2+
-
5 min at 50C, 10 mM, 15.9% inhibition; 5 min at 50C, 1 mM, 12.5% inhibition
Ca2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ca2+
Cu2+
-
5 min at 50C, 10 mM, 72.0% inhibition; 5 min at 50C, 1 mM, 67.8% inhibition
Cu2+
-
competitive inhibition, 26.7% residual activity at 10 mM
D-cellobiose
-
competitive inhibition
D-cellobiose
-
competitive inhibition
D-glucono-1,5-lactone
Penicillium funiculosum
-
-
D-glucose
-
competitive inhibition
D-glucose
Penicillium funiculosum
-
-
D-glucose
Q53EH2
at 20 mM
D-glucose
-
D-glucose inhibition is competitive for 4-nitrophenyl beta-D-glucopyranoside
EDTA
-
10% of enzyme inhibition at 20 mM, at 37C, pH 6.5
EGTA
-
10% of enzyme inhibition at 20 mM, at 37C, pH 6.5
Fe3+
-
80.5% residual activity at 10 mM
-
Hg2+
Gluconacetobacter xylinus
-
slight inhibition
Hg2+
-
5 min at 50C, 10 mM, 100.0% inhibition; 5 min at 50C, 1 mM, 100.0% inhibition
Hg2+
-
competitive inhibition, 4.3% residual activity at 10 mM
KCl
-
25% of enzyme inhibition at 0.5 mM, at 37C, pH 6.5
Mg2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Mg2+
N-bromosuccinimide
Gluconacetobacter xylinus
-
-
N-bromosuccinimide
-
-
NaCl
-
25% of enzyme inhibition at 0.5 mM, at 37C, pH 6.5
Ni2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ni2+
o-phthaldialdehyde
Thermomonospora sp.
-
-
p-chloromercuribenzoate
-
-
SDS
Gluconacetobacter xylinus
-
-
xylobiosyl-deoxynojirimycin
-
X2DNJ
xylobiosyl-isofagomine
-
X2IF
xylobiosyl-isofagomine lactam
-
X2IL
Zn2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Zn2+
additional information
B0FWI3
Cel9D has 27% lower activity on medium-viscosity carboxymethyl cellulose compared to the activity on low-viscosity carboxymethyl cellulose. Is not inhibited by 10 mM EDTA
-
additional information
-
not inhibited by EDTA, N-ethylmaleimide, beta-mercaptoethanol or cysteine. Ca2+, Co2+, Mg2+, Mn2+, and Zn2+ have no inhibitory effect
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
ethanol
-
5% (v/v) ethanol increases the enzyme activity about 30%
ethanol
G3BJX3, G3BJX4
5% (v/v), 158.4% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42C
methanol
-
5% (v/v) methanol increases the enzyme activity about 30%
methanol
G3BJX3, G3BJX4
5% (v/v), 141% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42C
additional information
B0FWI3
calcium ions are not required for its function
-
additional information
-
thiol groups are not essential for catalytic activity, the enzyme is not affected by Tween 20
-
additional information
-
wild type beta-glucosidase and its mutant S319C enhance activities of endo/exocellulase Cel9A on carboxymethyl cellulose and filter paper substrates
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.74
-
4-nitrophenyl beta-D-cellobioside
-
at 65C in pH 5.5 citrate buffer
0.22
-
4-nitrophenyl beta-D-glucopyranoside
G3BJX3, G3BJX4
pH 4.6, 42C
1.06
-
4-nitrophenyl beta-D-glucopyranoside
G3BJX3, G3BJX4
pH 4.6, 40C
2.5
-
4-nitrophenyl beta-D-glucopyranoside
-
in 100 mM sodium acetate buffer (pH 4.5), at 65C
0.65
-
alpha-cellulose
-
pH 5.0
-
0.461
-
cellobiose
-
at 40C, pH 4.9
1.2
-
cellobiose
-
-
1.26
-
cellobiose
-
at 50C, pH 4.9
2.67
-
cellobiose
-
-
2.89
-
cellobiose
-
at 60C, pH 4.9
2.9
-
cellobiose
Q53EH2
-
3.12
-
cellobiose
-
at 70C, pH 4.9
6.82
-
cellobiose
-
value extrapolated to 70C
6.96
-
cellobiose
-
pH 6.5, at 37C
10
-
cellobiose
Penicillium funiculosum
-
-
12.5
-
cellobiose
-
-
13.5
-
cellobiose
-
-
26
-
cellobiose
-
-
86
-
cellobiose
-
in 100 mM sodium acetate buffer (pH 4.5), at 65C
0.028
-
Cellohexaose
-
-
0.29
-
Cellohexaose
-
-
0.37
-
Cellopentaose
Penicillium funiculosum
-
-
0.41
-
Cellopentaose
-
-
6
-
Cellopentaose
-
-
0.77
-
Cellotetraose
Penicillium funiculosum
-
-
0.89
-
Cellotetraose
-
-
1.1
-
Cellotetraose
Q53EH2
-
6.7
-
Cellotetraose
-
-
0.44
-
cellotriose
Penicillium funiculosum
-
-
0.97
-
cellotriose
-
-
1.7
-
cellotriose
Q53EH2
-
3.7
-
cellotriose
Gluconacetobacter xylinus
-
-
8.7
-
cellotriose
-
-
127
-
cellulose
Penicillium funiculosum
-
H3OP4 swollen
0.77
-
o-nitrophenyl beta-D-glucopyranoside
Penicillium funiculosum
-
-
0.45
-
p-nitrophenyl glucoside
-
pH 6.5, at 37C
0.7
-
p-nitrophenyl-beta-D-cellobioside
-
-
0.7
-
p-nitrophenyl-beta-D-cellobioside
-
-
2.8
-
p-nitrophenyl-beta-D-glucopyranoside
-
-
4
-
p-nitrophenyl-beta-D-glucopyranoside
-
-
0.42
-
p-nitrophenyl-beta-D-glucoside
-
-
2.2
-
p-nitrophenyl-beta-D-glucoside
-
-
10.7
-
Gentiobiose
-
-
additional information
-
additional information
Penicillium funiculosum
-
Walseth cellulose 10 mg/ml, cellopentaose 0.83 mg/ml, cellotriose 0.55 mg/ml, cellotriose 0.55 mg/ml
-
additional information
-
additional information
-
laminarioligosaccharide degree of polymerization 2, 5.37, laminarioligosaccharide degree of polymerization 3, 2.77, laminarioligosaccharide degree of polymerization 4, 0.52
-
additional information
-
additional information
-
cellobiose 15 mg/ml
-
additional information
-
additional information
-
Michaelis-Menten kinetic
-
TURNOVER NUMBER [1/s]
TURNOVER NUMBER MAXIMUM[1/s]
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
0.00039
-
2,4-dinitrophenol cellobiose
Q9XCD4
pH 5.5
69.6
-
4-nitrophenyl beta-D-cellobioside
-
at 65C in pH 5.5 citrate buffer
126.6
-
4-nitrophenyl beta-D-glucopyranoside
G3BJX3, G3BJX4
pH 4.6, 40C
203
-
4-nitrophenyl beta-D-glucopyranoside
G3BJX3, G3BJX4
pH 4.6, 42C
4945
-
4-nitrophenyl beta-D-glucopyranoside
-
in 100 mM sodium acetate buffer (pH 4.5), at 65C
32
-
cellobiose
-
-
281
-
cellobiose
-
in 100 mM sodium acetate buffer (pH 4.5), at 65C
kcat/KM VALUE [1/mMs-1]
kcat/KM VALUE [1/mMs-1] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
40.1
-
4-nitrophenyl beta-D-cellobioside
-
at 65C in pH 5.5 citrate buffer
214148
11900
-
4-nitrophenyl beta-D-glucopyranoside
G3BJX3, G3BJX4
pH 4.6, 40C
80189
915700
-
4-nitrophenyl beta-D-glucopyranoside
G3BJX3, G3BJX4
pH 4.6, 42C
80189
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
29
-
D-glucose
-
in 100 mM sodium acetate buffer (pH 4.5), at 65C
0.0058
-
xylobiosyl-deoxynojirimycin
-
wild type
0.018
-
xylobiosyl-deoxynojirimycin
-
Q87M mutant
0.062
-
xylobiosyl-deoxynojirimycin
-
Q87Y mutant
0.18
-
xylobiosyl-deoxynojirimycin
-
N44A mutant
0.00013
-
xylobiosyl-isofagomine
-
wild type
0.00039
-
xylobiosyl-isofagomine
-
Q87M mutant
0.0012
-
xylobiosyl-isofagomine
-
Q87Y mutant
0.0071
-
xylobiosyl-isofagomine
-
N44A mutant
0.00034
-
xylobiosyl-isofagomine lactam
-
wild type
0.0021
-
xylobiosyl-isofagomine lactam
-
Q87M mutant
0.011
-
xylobiosyl-isofagomine lactam
-
Q87Y mutant
0.024
-
xylobiosyl-isofagomine lactam
-
N44A mutant
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
0.0011
-
-
P-42, cotton, pH 5, at 30C
0.0012
-
-
P-42, avicel, pH 5, at 30C
0.0021
-
-
P-42, bacterial cellulose, pH 5, at 30C
0.0142
-
-
Ex-1, cotton, pH 5, at 30C
0.0145
-
-
Ex-1, bacterial cellulose, pH 5, at 30C
0.0209
-
-
Ex-1, avicel, pH 5, at 30C
0.0243
-
-
Ex-1, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30C
0.0244
-
-
P-42, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30C
0.025
-
-
cellulose MN300
0.026
-
-
Ex-1, carboxymethyl cellulose, pH 5, at 30C
0.03
-
-
cellulose HBS
0.0311
-
-
P-42, carboxymethyl cellulose, pH 5, at 30C
0.04
-
-
using filter paper as substrate, at 60C in pH 6.5 citrate buffer
0.041
-
-
avicel
0.067
-
-
crude enzyme from homogenate, using cellobiose as substrate, pH and temperature not specified in the publication
0.102
-
-
H3PO4 swollen avicel
0.108
-
-
HCl swollen avicel
0.128
-
-
celloheptaose
0.2
-
-
using avicel as substrate, at 60C in pH 6.5 citrate buffer
0.228
-
-
after 3.4fold purification, using cellobiose as substrate, pH and temperature not specified in the publication
0.236
-
-
cellohexaose
0.25
-
B0FWI3
with amorphous cellulose
0.275
-
B0FWI3
with carboxymethyl cellulose
0.323
-
-
pH 6.5, at 37C
0.35
-
G3BJX3, G3BJX4
2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 40C
0.42
-
-
cellopentaose
0.65
-
G3BJX3, G3BJX4
2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 42C
0.684
-
-
cellotetraose
1.9
-
-
sigmacell, pH 5.0
2.53
-
-
cellulose acetate, pH 5.0
3.36
-
-
cellulose, pH 5.0
3.583
-
-
alpha-cellulose, pH 5.0
5.3
-
G3BJX3, G3BJX4
cellobiose as substrate, pH 4.6, 42C
7
-
Penicillium funiculosum
-
-
11.5
-
G3BJX3, G3BJX4
4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 42C
14.5
-
-
bacterial cellulose powder, not dried, isoenzyme II
15.1
-
-
bacterial cellulose powder, not dried, isoenzyme I
20.9
-
-
avicel, isoenzyme II
21.1
-
-
avicel, isoenzyme I; isoenzyme I
23.5
-
-
using phosphate acid swollen cellulose as substrate, at 60C in pH 6.5 citrate buffer
25.5
-
-
carboxymethyl cellulose, isoenzyme I
26
-
-
carboxymethyl cellulose, isoenzyme II
26
-
G3BJX3, G3BJX4
4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
27.1
-
-
p-nitrophenyl beta-D-lactoside, isoenzyme II
27.3
-
-
p-nitrophenyl beta-D-lactoside, isoenzyme I
33.2
-
-
p-nitrophenyl beta-D-celloside, isoenzyme II
33.5
-
G3BJX3, G3BJX4
cellobiose as substrate, pH 4.6, 40C
34
-
-
p-nitrophenyl beta-D-celloside, isoenzyme I
41
-
G3BJX3, G3BJX4
4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 40C
52.9
-
-
using 4-nitrophenyl beta-D-cellobioside as substrate, at 65C in pH 5.5 citrate buffer
58.79
-
Thermomonospora sp.
-
carboxymethylcellulase activity
77
-
G3BJX3, G3BJX4
4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
129
-
Q53EH2
p-nitrophenyl-beta-D-fucopyranoside as substrate
130
-
Q53EH2
o-nitrophenyl-beta-D-galactopyranoside as substrate; p-nitrophenyl-beta-D-cellobioside as substrate
133
-
Q53EH2
p-nitrophenyl-beta-D-galactopyranoside as substrate
177
-
Q53EH2
p-nitrophenyl-beta-D-glucopyranoside as substrate
220
-
-
using carboxymethylcellulose as substrate, at 65C in pH 5.5 citrate buffer
693
-
-
using lichenan as substrate, at 60C in pH 6.5 citrate buffer
717
-
-
using barley glucan as substrate, at 60C in pH 6.5 citrate buffer
additional information
-
Sporotrichum pulverulentum
-
-
additional information
-
Gluconacetobacter xylinus
-
840 U/mg
additional information
-
-
20.8 OD405/mg
additional information
-
Q9XCD4
cloned Cel48A, 0.068 mol/min/mol, filter paper, pH 5.5, at 50C, liquid assays; cloned Cel48A, 0.191 mol/min/mol, bacterial microcrystalline cellulose, pH 5.5, at 50C, liquid assays; cloned Cel48A, 0.288 mol/min/mol, swollen cellulose, pH 5.5, at 50C, thin-layer analysis; cloned Cel48A, 0.292 mol/min/mol, carboxymethyl cellulose, pH 5.5, at 50C, liquid assays; cloned Cel48A, 0.405 mol/min/mol, swollen cellulose, pH 5.5, at 50C, liquid assays; cloned Cel48A, approximately 16 mol/min/mol, cellotetraose, pH 5.5, at 50C, thin-layer analysis; cloned Cel48A, approximately 9 mol/min/mol, cellopentaose, pH 5.5, at 50C, thin-layer analysis; native Cel48A, 17.9 mol/min/mol, swollen cellulose, pH 5.5, at 50C; native Cel48A, 3.7 mol/min/mol, carboxymethyl cellulose, pH 5.5, at 50C
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4.6
-
G3BJX3, G3BJX4
assay at, optimal for 4-nitrophenol beta-D-glucopyranoside as substrate; assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside as substrate
5
-
Penicillium funiculosum
-
-
5
-
-
both isoenzymes
5
-
-
Ex-1, carboxymethyl cellulose as substrate; P-42, carboxymethyl cellulose as substrate
5.2
-
-
at 50C
5.5
6.5
-
using 4-nitrophenyl beta-D-cellobioside as substrate
5.5
-
Gluconacetobacter xylinus
-
-
6
6.5
Q9XCD4
50C
6.5
-
-
using carboxymethylcellulose as substrate
7
-
Thermomonospora sp.
-
for both cellulase and xylanase activity
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
5
-
84% and 86% of the maximum activity appears at pH 4.0 and 5.0, respectively
5
9
Q9XCD4
retaines more than 50% of activity, 50C
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
-
Gluconacetobacter xylinus
-
-
40
-
G3BJX3, G3BJX4
assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside
42
-
G3BJX3, G3BJX4
assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside as substrate
45
-
-
pH 5.2
50
-
Penicillium funiculosum
-
-
50
-
-
both isoenzymes
50
-
-
Ex-1, carboxymethyl cellulose as substrate; P-42, carboxymethyl cellulose as substrate
50
-
Thermomonospora sp.
-
-
60
-
-
using carboxymethylcellulose as substrate
65
-
-
using 4-nitrophenyl beta-D-cellobioside as substrate
85
-
-
avicelase activity
95
100
-
carboxymethyl cellulase activity
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
37
-
stable for at least 1 h
40
-
-
incubation for 3 h, 40% loss of enzyme activity
60
70
-
85% and 91% of the maximum activity at 60C and 70C, respectively
70
-
-
incubation for 3 h, 100% loss of enzyme activity
pI VALUE
pI VALUE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
4
-
Q9XCD4
IEF gel
4.33
-
Thermomonospora sp.
-
isoelectric focusing
4.9
-
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
SOURCE
-
Driselase, from Irpex lacteus
Manually annotated by BRENDA team
Thermomonospora sp.
-
-
Manually annotated by BRENDA team
Sporotrichum pulverulentum
-
-
Manually annotated by BRENDA team
Penicillium funiculosum
-
-
Manually annotated by BRENDA team
Gluconacetobacter xylinus
-
-
Manually annotated by BRENDA team
Gluconacetobacter xylinus BPR2001, Hypocrea jecorina QM 9414, Vibrio cholerae E1 Tor N16961
-
-
-
Manually annotated by BRENDA team
additional information
G3BJX3, G3BJX4
isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens; isolated from rumen from a yak rumen metagenomic library, chinese yak, Bos grunniens
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
expressed in Escherichia coli
Manually annotated by BRENDA team
Vibrio cholerae E1 Tor N16961
-
-
-
Manually annotated by BRENDA team
Sporotrichum pulverulentum
-
-
-
Manually annotated by BRENDA team
Penicillium funiculosum
-
-
-
Manually annotated by BRENDA team
Gluconacetobacter xylinus
-
-
-
Manually annotated by BRENDA team
Thermomonospora sp.
-
-
-
Manually annotated by BRENDA team
Gluconacetobacter xylinus BPR2001, Hypocrea jecorina QM 9414
-
-
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
42000
-
-
P-42, SDS-PAGE
48600
-
Sporotrichum pulverulentum
-
ultracentrifugation
51570
-
Q53EH2
calculated
53000
-
-
isoenzyme II
53000
-
-
Ex-1, SDS-PAGE
56000
-
-
isoenzyme I
62000
-
-
calculation from sequence of amino acids
64000
-
-
gel filtration
65000
-
Penicillium funiculosum
-
gel filtration
65000
-
-
SDS-PAGE
77000
-
B0FWI3
sequence analysis
88000
-
-
SDS-PAGE
130300
-
-
gel filtration
151000
-
-
gel filtration
169300
-
-
calculated from amino acid sequence
200000
-
-
gel filtration
780000
-
-
gel filtration
additional information
-
-
70000 g/mol
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
Penicillium funiculosum
-
x * 20000, SDS-PAGE
?
Gluconacetobacter xylinus
-
x * 81200, SDS-PAGE
?
-
x * 112000, SDS-PAGE
?
-
23000, SDS-PAGE
?
-
x * 80000, SDS-PAGE
?
-
x * 85000, SDS-PAGE
?
-
x * 29000, SDS-PAGE
?
-
x * 58000, SDS-PAGE
?
-
x * 47300, nonglycosylated form, SDS-PAGE; x * 49300, glycosylated form, SDS-PAGE
?
-
x * 87000, SDS-PAGE
?
-
x * 87000, SDS-PAGE
?
Thermomonospora sp.
-
x * 14200, SDS-PAGE
?
-
x * 38400, calculated from amino acid sequence; x * 40000, SDS-PAGE
?
Gluconacetobacter xylinus BPR2001
-
x * 81200, SDS-PAGE
-
?
Ruminococcus albus AR67
-
x * 58000, SDS-PAGE
-
?
-
x * 112000, SDS-PAGE
-
dimer
-
2 * 83500
dimer
-
alpha,beta, 2 * 100000, SDS-PAGE
homooctamer
-
8 * 98000, SDS-PAGE
homooctamer
Stereum hirsutum SKU512
-
8 * 98000, SDS-PAGE
-
monomer
B0FWI3
1 * 76000, SDS-PAGE
additional information
Thermomonospora sp.
-
fluorescence study on interactions of alpha-crystallin with the molten globule state of the enzyme induced by guanidine hydrochloride
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
no modification
-
-
no modification
Aspergillus fumigatus 155
-
-
-
no modification
-
-
no modification
-
-
no modification
-
-
proteolytic modification
-
Papain digestion of native Ex-1, core fragment obtaining
glycoprotein
-
-
glycoprotein
Stereum hirsutum SKU512
-
-
-
no modification
Q9XCD4
-
no modification
-
-
no modification
Vibrio cholerae E1 Tor N16961
-
-
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
resolution 2.0 A
-
combination of the immunoglobulin-like module and the catalytic module and complex of inactive mutant with cellotetraose, modified microbatch crystallization under oil at 0C
-
molecular docking studies for the enzyme with the substrates cellobiose and 4-nitrophenyl-beta-D-glucopyranoside. The essential catalytic residues of RuBG3A are D297 and E499. Besides D113, K218, H219, and R185 also form hydrogen bonds with substrates and are condervative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters; molecular docking studies for the enzyme with the substrates cellobiose and 4-nitrophenyl beta-D-glucopyranoside. The essential catalytic residues of RuBG3B are D272 and E481. Besides D88, K193, H194, and R160 also form hydrogen bonds with substrates and are conservative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent with the kinetic parameters
G3BJX3, G3BJX4
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
2
5.5
-
after 24 h of incubation at 20C, the enzyme is fairly stable at the acidic pH ranging from 3.0 to 5.5. About 15% and 76% of activity remain after incubation at pH 2.0 and 2.5, respectively
3
5
-
isoenzyme II
3
7
-
Ex-1, retains more than 80% of its maximum activity
3
9
-
isoenzyme I
3.6
5.7
-
analysis of influence of sodium citrate buffer pH
3.6
7
Gluconacetobacter xylinus
-
-
5
5.5
-
for 1 h, at 50C
5
6
-
P-42, retains more than 70% of its maximum activity
5
8
Thermomonospora sp.
-
50C, 1 h, less than 15% loss of activity
5
8
Q53EH2
when incubated at 40C for 12 h with no substrate
6
7.5
-
most stable at pH 6-7.5
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
40
-
P-42, pH 5.0, retains more than 80% of its maximum activity
25
55
-
Ex-1, pH 5.0, retains more than 80% of its maximum activity
30
45
-
for 1 h, pH 5.2
30
-
-
stable below 30C
30
-
Gluconacetobacter xylinus
-
inactivated above 50C; stable below 30C
30
-
-
stable below 30C
37
-
-
10% activity loss after 3 h at 37C
37
-
-
20% activity loss after 10 min, 75% activity loss after 15 h at 37C
40
50
Q9XCD4
decreases markedly above 50C
40
60
-
loses none of its activity for a period of up to 5 h
43
-
G3BJX3, G3BJX4
Tm-value
45
-
G3BJX3, G3BJX4
40% activity retained after incubation for 1 h; stable after 1 h incubation
50
70
-
at 70C, 65C, 60C, and 50C the enzyme retains 50% of the initial activity after 2, 10, 16, and 35 min, respectively
50
-
-
loses less than 3% activity after 7 hours at 50C
50
-
Penicillium funiculosum
-
stable at 50C, activity loss above 70C
50
-
-
both isoenzymes stable below 50C
50
-
Thermomonospora sp.
-
15 h, enzyme retains complete activity of hydrolyzing xylan and carboxymethylcellulose
50
-
G3BJX3, G3BJX4
Tm-value, retained 50% activity after 20 min incubation
55
-
-
stable below 55C
60
-
-
the melting temperature of the enzyme is at 60C
70
-
-
undergoes considerable deactivation within quite a short time
80
-
Thermomonospora sp.
-
half-life: 20 min
95
-
-
50% activity loss after 30 min at 95C
ORGANIC SOLVENT
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ethanol
G3BJX3, G3BJX4
5% (v/v), 100.8% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5% (v/v), 158.4% activity compared to activity without addition of ethanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42C
Methanol
G3BJX3, G3BJX4
5% (v/v), 108.2% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5% (v/v), 141% activity compared to activity without addition of methanol, 1.25 mM 4-nitrophenyl-beta-D-glucopyranoside as substrate, pH 4.6, 42C
STORAGE STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
-20C or -80C, 0.5 mM dithiothreitol
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
catalytic and cellulose binding domains by cellulose FPLC column chromatography, ultrafiltration and papain treatment
-
precipitation with acetone, gel filtration chromatography on Sephadex G-100 and DEAE-cellulose chromatography, 47.14fold
-
Ni-nitrilotriacetatic acid agarose column chromatography
Q53EH2
by nickel affinity chromatography and gel filtration chromatography
-
wild-type and mutants purified by immobilized metal affinity chromatography. Wild-type further purified by gel filtration
B0FWI3
ammonium sulfate precipitation, Macro-Prep DEAE column chromatography, methyl hydrophobic-interaction column chromatography, and Superdex 200 gel filtration
-
3368fold
Gluconacetobacter xylinus
-
gel filtration
-
-
Penicillium funiculosum
-
15fold
Sporotrichum pulverulentum
-
Superdex 200 gel filtration
-
cloned Cel48A, centrifugation, phenylmethanesulfonyl fluoride and ammonium sulfate fractionation, phenyl-Sepharose column, Q-Sepharose column; native Cel48A, HPLC
Q9XCD4
-
Thermomonospora sp.
-
TSC by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration
Thermomonospora sp.
-
Ni-NTA resincolumn chromatography
-
recombinant enzyme; recombinant enzyme
G3BJX3, G3BJX4
centrifugation, streptomycin sulfate precipitation, ammonium sulfate fractionation, DEAE column chromatography, IMAC-Zn2+ chromatography
-
Cloned/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Escherichia coli
-
Escherichia coli
-
expression in Escherichia coli
-
expression in Escherichia coli
Q53EH2
into vector pET30a and expressed in Escherichia coli BL21(DE3)
B0FWI3
Escherichia coli
-
Escherichia coli
-
Escherichia coli
-
Escherichia coli
-
Escherichia coli and Streptomyces lividans
-
Escherichia coli and Streptomyces lividans, expression in Streptomyces lividans
Q9XCD4
expressed in Escherichia coli and purified; expressed in Escherichia coli and purified
G3BJX3, G3BJX4
expressed in Escherichia coli BL21(DE3) cells
-
overexpression in Escherichia coli
-
ENGINEERING
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
N44A
-
based on the pUC12-1.1Cex PTIS plasmid
Q87M
-
based on the pUC12-1.1Cex PTIS plasmid
Q87Y
-
based on the pUC12-1.1Cex PTIS plasmid
E795Q
-
inactive enzyme
D166A
B0FWI3
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
DELTAS168
B0FWI3
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
E612A
B0FWI3
no activity on either 1% (wt/vol) carboxymethyl cellulose after 4 h of incubation or 5 mM cellopentaose after 24 h of incubation
S168A
B0FWI3
retains about 40% of activity compared to the wild-type
A229V
-
loop mutant, weaker binding affinities to all tested ligands
E495D
-
active site mutant, weaker binding affinities to all tested ligands
E495N
-
active site mutant, weaker binding affinities to all tested ligands
G234A
-
loop mutant, weaker binding affinities to all tested ligands
G234D
-
loop mutant, weaker binding affinities to all tested ligands
G234N
-
loop mutant, weaker binding affinities to all tested ligands
G234S
-
loop mutant, higher binding affinities for 4-methylumbelliferyl beta-cellotrioside and cellotriose
G234S/G284P
-
loop mutant, higher binding affinity for 4-methylumbelliferyl beta-cellotrioside
G284A
-
loop mutant, weaker binding affinities to all tested ligands
G284I
-
loop mutant, weaker binding affinities to all tested ligands
G284P
-
loop mutant, weaker binding affinities to all tested ligands
G284P/A229V
-
loop mutant, weaker binding affinities to all tested ligands
G331A
-
active site mutant, weaker binding affinities to all tested ligands
G496P
-
loop mutant, weaker binding affinities to all tested ligands
H326A
-
active site mutant, weaker binding affinities to all tested ligands
H326S
-
active site mutant, higher binding affinities for 4-methylumbelliferyl beta-cellobioside and cellotriose
L230C
-
loop mutant, weaker binding affinities to all tested ligands
L230C/D512C
-
loop mutant, weaker binding affinities to all tested ligands
N233C
-
loop mutant, weaker binding affinities to all tested ligands
N233C/D506C
-
loop mutant, weaker binding affinities to all tested ligands
W329C
-
active site mutant, weaker binding affinities to all tested ligands
W332A
-
active site mutant, weaker binding affinities to all tested ligands
W332Y
-
active site mutant, weaker binding affinities to all tested ligands
Renatured/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
denaturation studies using GdnCl indicate that TSC folds through a partially folded state that resembles molten globule at 1.8 M GdnCl. alpha-Crystallin chaperone-mediated in vitro folding, molecular mechanism, overview. Reconstitution of the active TSC is observed in 50 mM sodium phosphate buffer, pH 7.0, on cooling the alpha-crystallin-TSC-m complex to 4C. Addition of alpha-crystallin to the molten globule-like intermediate of TSC complex initiates the refolding of TSC with 69% recovery of the biological activity of the enzyme
Thermomonospora sp.
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
analysis
-
tandem mass spectrometry to compare gas-phase and solution binding of small-molecule inhibitors to the wild type and three mutant forms of Cex
energy production
-
ethanol production from concentrated agricultural waste corncob
energy production
Hypocrea jecorina ZU-02
-
ethanol production from concentrated agricultural waste corncob
-
biotechnology
-
hydrolysis of lignocellulosic materials
additional information
-
obtaining of renewable energy