Information on EC 3.2.1.74 - glucan 1,4-beta-glucosidase

Word Map on EC 3.2.1.74
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.74
-
RECOMMENDED NAME
GeneOntology No.
glucan 1,4-beta-glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->4)-linkages in (1->4)-beta-D-glucans, to remove successive glucose units
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Metabolic pathways
-
-
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
4-beta-D-glucan glucohydrolase
Acts on 1,4-beta-D-glucans and related oligosaccharides. Cellobiose is hydrolysed, but very slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
37288-52-1
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
aff. tenuissima, GenBank Accession: HQ630970
-
-
Manually annotated by BRENDA team
aff. phaeospermum, GenBank Accession: HQ630967; aff. sacchari, GenBank Accession: HQ630961
-
-
Manually annotated by BRENDA team
strain 155 v cellulolytic fungus
-
-
Manually annotated by BRENDA team
strain 155 v cellulolytic fungus
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
aff. fumigatus, GenBank Accession: HQ631021
-
-
Manually annotated by BRENDA team
aff. pullans, GenBank Accession: HQ631013
-
-
Manually annotated by BRENDA team
GenBank Accession: HQ631009
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Cephalosporium sp.
aff. gramineum, GenBank Accession: HQ630973
-
-
Manually annotated by BRENDA team
GenBank Accession: HQ630978
-
-
Manually annotated by BRENDA team
aff. cladosporioides, GenBank Accession: HQ630971
-
-
Manually annotated by BRENDA team
ATCC35296
Uniprot
Manually annotated by BRENDA team
aff. bassiana, GenBank Accession: HQ630968
-
-
Manually annotated by BRENDA team
GenBank Accession: HQ631008
-
-
Manually annotated by BRENDA team
aff. nigrum, GenBank Accession: HQ630972
-
-
Manually annotated by BRENDA team
aff. spinifera, GenBank Accession: HQ631027
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
aff. moniliformis, GenBank Accession: HQ630966
-
-
Manually annotated by BRENDA team
Gilmaniella humicola
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
BPR2001
-
-
Manually annotated by BRENDA team
aff. bolleyi, GenBank Accession: HQ630981
-
-
Manually annotated by BRENDA team
GenBank Accession: HQ630974
-
-
Manually annotated by BRENDA team
Myrothecium striatisporum
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
aff. oryzae, GenBank Accession: HQ630982
-
-
Manually annotated by BRENDA team
aff. minioluteum, GenBank Accession: HQ631007
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
subsp. cellulosa
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
aff. lignivora, GenBank Accession: HQ630984
-
-
Manually annotated by BRENDA team
Sporotrichum pulverulentum
also called Chrysosporium lignorum
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
Thermomonospora sp.
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
QM 9414
-
-
Manually annotated by BRENDA team
ZU-02
-
-
Manually annotated by BRENDA team
aff. atroviride, GenBank Accession: HQ630969; aff. koningii, GenBank Accession: HQ630959
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
from yak rumen
-
-
Manually annotated by BRENDA team
strain E1 Tor N16961
-
-
Manually annotated by BRENDA team
strain E1 Tor N16961
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,4-dinitrophenol cellobiose + H2O
?
show the reaction diagram
-
-
-
?
2-nitrophenyl beta-cellobioside + H2O
2-nitrophenol + beta-cellobiose
show the reaction diagram
-
3.1% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
show the reaction diagram
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
show the reaction diagram
4-methylumbelliferryl 4-O-(beta-D-glucopyranosyl)-beta-D-xylopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-cellobioside + H2O
?
show the reaction diagram
4-methylumbelliferryl-beta-cellotrioside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-D-cellotrioside + H2O
?
show the reaction diagram
-
-
-
?
4-methylumbelliferryl-beta-D-glucopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
4-methylumbelliferryl-beta-D-glucoside + H2O
?
show the reaction diagram
4-methylumbelliferyl beta-D-cellobioside + H2O
D-glucose + ?
show the reaction diagram
i.e.MuC, 14.9% relative activity compared to 4-nitrophenyl-beta-D-glucopyranoside as substrate
-
-
?
4-methylumbelliferyl-beta-D-cellobioside + H2O
?
show the reaction diagram
-
-
-
?
4-methylumbelliferyl-beta-D-cellobioside + H2O
D-glucose + ?
show the reaction diagram
i.e.MuC, 157.7% relative activity compared to 4-nitrophenyl beta-D-glucopyranoside as substrate
-
-
?
4-methylumbelliferyl-beta-D-lactoside + H2O
4-methylumbelliferone + D-lactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-cellobioside + H2O
4-nitrophenol + beta-cellobiose
show the reaction diagram
-
6.5% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + beta-D-cellobiose
show the reaction diagram
-
exo-beta-1,4-glucanase activity, 24.1% activity compared to carboxymethylcellulose
-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactopyranose
show the reaction diagram
-
1.2% activity compared to 4-nitrophenyl beta-D-glucopyranoside
-
-
?
4-nitrophenyl beta-D-glucopyranose + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
?
acid swollen cellulose + H2O
D-glucose + ?
show the reaction diagram
-
-
-
?
acid-swollen cellulose + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
alpha-cellulose + H2O
?
show the reaction diagram
-
no activity with carboxymethylcellulose, salicin, cellobiose and alpha-dimethylglucoside
-
-
?
amorphous cellulose + H2O
?
show the reaction diagram
-
-
-
?
amygdalin + H2O
?
show the reaction diagram
-
-
-
-
?
arbutin + H2O
?
show the reaction diagram
-
-
-
-
?
avicel + H2O
?
show the reaction diagram
avicel + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
avicel + H2O
cellobiose + ?
show the reaction diagram
-
0.09% activity compared to carboxymethylcellulose
-
-
?
Avicel + H2O
D-glucose + cellotetraose + cellotriose + cellobiose
show the reaction diagram
bacterial cellulose + H2O
?
show the reaction diagram
-
-
-
-
?
bacterial microcrystalline cellulose + H2O
?
show the reaction diagram
-
-
-
?
barley glucan + H2O
?
show the reaction diagram
-
326% activity compared to carboxymethylcellulose
-
-
?
beta-1,4-xylan + H2O
?
show the reaction diagram
-
-
-
-
?
beta-glucan + H2O
?
show the reaction diagram
-
from yeast and from barley
-
-
?
carboxymethyl cellulose + H2O
?
show the reaction diagram
carboxymethyl cellulose + H2O
cellobiose + cellopentaose + cellotetraose + cellotriose + D-glucose
show the reaction diagram
-
-
the major product is cellobiose with some minor products
-
?
carboxymethylcellulose + H2O
?
show the reaction diagram
carboxymethylcellulose + H2O
D-glucose + cellooligosaccharides
show the reaction diagram
Thermomonospora sp.
-
-
-
-
?
cellobiose + H2O
2 beta-D-glucose
show the reaction diagram
cellobiose + H2O
2 D-glucose
show the reaction diagram
cellobiose + H2O
D-glucose
show the reaction diagram
cellobiose + H2O
D-glucose + cellotetraose + cellotriose
show the reaction diagram
cellodextrins + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
celloheptaose + H2O
?
show the reaction diagram
cellohexaose + H2O
cellopentaose + D-glucose
show the reaction diagram
-
-
-
?
cellohexaose + H2O
D-glucose
show the reaction diagram
cellooligomers + H2O
cellobiose + cellooligomers
show the reaction diagram
-
-
-
ir
cellooligomers + H2O
D-glucose
show the reaction diagram
cellooligomers + H2O
D-glucose + cellooligomers
show the reaction diagram
-
-
-
ir
cellopentaose + H2O
?
show the reaction diagram
cellopentaose + H2O
D-glucose
show the reaction diagram
cellopentaose + H2O
glucose
show the reaction diagram
cellotetraose + 3 H2O
4 D-glucose
show the reaction diagram
cellotetraose + H2O
?
show the reaction diagram
cellotetraose + H2O
cellobiose + cellotriose + D-glucose
show the reaction diagram
-
-
cellobiose is the dominant product, while cellotriose and glucose are minor products
-
?
cellotetraose + H2O
D-glucose + cellotriose + cellobiose
show the reaction diagram
cellotetraose + H2O
glucose + cellobiose + cellotriose
show the reaction diagram
-
with a conversion ratio of 80%
-
?
cellotriose + H2O
D-glucose
show the reaction diagram
cellotriose + H2O
D-glucose + cellobiose
show the reaction diagram
cellotriose + H2O
glucose
show the reaction diagram
cellulose + H2O
?
show the reaction diagram
cellulose + H2O
D-glucose
show the reaction diagram
cellulose + H2O
D-glucose + ?
show the reaction diagram
cellulose acetate + H2O
?
show the reaction diagram
-
-
-
-
?
cotton + H2O
?
show the reaction diagram
-
-
-
-
?
D(+)-cellobiose + H2O
D-glucose
show the reaction diagram
-
-
-
-
r
esculin + H2O
?
show the reaction diagram
-
-
-
-
?
filter paper
?
show the reaction diagram
-
-
-
?
filter paper + H2O
cellobiose
show the reaction diagram
-
-
-
-
?
filter paper + H2O
cellobiose + ?
show the reaction diagram
-
0.02% activity compared to carboxymethylcellulose
-
-
?
filter paper + H2O
cellobiose + cellopentaose + cellotetraose + cellotriose + D-glucose
show the reaction diagram
-
-
the major product is cellobiose with some minor products
-
?
gentiobiose + H2O
?
show the reaction diagram
hydroquinone-beta-D-glucopyranoside + H2O
hydroquinone + D-glucose
show the reaction diagram
-
-
-
?
laminaribiose + H2O
?
show the reaction diagram
laminarin + H2O
?
show the reaction diagram
-
-
-
-
?
laminaritriose + H2O
?
show the reaction diagram
-
from barley
-
-
?
lichenan + H2O
?
show the reaction diagram
n-heptyl-beta-D-glucopyranoside + H2O
n-heptanol + D-glucose
show the reaction diagram
-
-
-
?
n-hexyl-beta-D-glucopyranoside + H2O
n-hexanol + D-glucose
show the reaction diagram
-
-
-
?
n-octyl-beta-D-glucopyranoside + H2O
n-octanol + D-glucose
show the reaction diagram
-
-
-
?
n-pentyl-beta-D-glucopyranoside + H2O
n-pentanol + D-glucose
show the reaction diagram
-
-
-
?
o-nitrophenyl beta-D-glucopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
o-nitrophenyl-beta-D-galactopyranose + H2O
o-nitrophenol + beta-D-galactopyranose
show the reaction diagram
p-nitrophenol beta-D-cellobioside + H2O
p-nitrophenol beta-D-glucopyranoside + D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl beta-D-lactoside + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl cellobioside + H2O
p-nitrophenyl + glucose
show the reaction diagram
p-nitrophenyl glucoside + H2O
p-nitrophenyl + glucose
show the reaction diagram
-
p-nitrophenyl releasing with no lag period, no activity with p-nitrophenyl-?-(N-acetylgalactosamine), p-nitrophenyl-?-glucose-6-phosphate, p-nitrophenyl-(N-acetylglucosamine), p-nitrophenyl-di(N-acetylglucosamine), p-nitrophenyl-tri(N-acetylglucosamine)
-
-
?
p-nitrophenyl-beta-D-cellobiose + H2O
p-nitrophenol + cellobioside
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-cellobioside + H2O
cellobiose + p-nitrophenol
show the reaction diagram
-
-
-
ir
p-nitrophenyl-beta-D-cellobioside + H2O
D-glucose + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
p-nitrophenyl-beta-D-cellotrioside + H2O
D-glucose + p-nitrophenyl-beta-D-cellobioside + p-nitrophenyl-beta-D-cellotetraoside + p-nitrophenyl-beta-D-glucoside
show the reaction diagram
p-nitrophenyl-beta-D-fucopyranoside + H2O
p-nitrophenol + fucopyranose
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
p-nitrophenyl-cellobioside + H2O
?
show the reaction diagram
-
-
-
-
?
phenyl-beta-D-glucopyranoside + H2O
phenol + D-glucose
show the reaction diagram
-
-
-
?
phosphate acid swollen cellulose + H2O
?
show the reaction diagram
-
10.7% activity compared to carboxymethylcellulose
-
-
?
prunasin + H2O
D-mandelonitrile + beta-D-glucose
show the reaction diagram
-
-
-
-
?
salicin + H2O
?
show the reaction diagram
sigmacell + H2O
?
show the reaction diagram
-
-
-
-
?
sophorose + H2O
?
show the reaction diagram
sugarcane bagasse + H2O
?
show the reaction diagram
swollen cellulose + H2O
cellobiose + cellotriose
show the reaction diagram
-
-
-
?
trehalose + H2O
?
show the reaction diagram
xylan + H2O
?
show the reaction diagram
xylo-oligosaccharides + H2O
?
show the reaction diagram
-
-
-
-
?
xylobiose + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
cellobiose + H2O
D-glucose
show the reaction diagram
-
hydrolysis of cellulose
-
-
ir
cellulose + H2O
?
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
5 mM, 107.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 92.8% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Co2+
-
5 min at 50C, 10 mM, 101.9% of the relative activity without metal ions; 5 min at 50C, 1 mM, 103.0% of the relative activity without metal ions
CoCl2
5 mM, 79.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 85.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
CuCl2
5 mM, 79.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 83.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-fructose
5 mM, 102.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-glucose
5 mM,82% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
D-xylose
5 mM, 106.3% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Fe3+
-
5 min at 50C, 10 mM, 103.6% of the relative activity without metal ions; 5 min at 50C, 1 mM, 105.5% of the relative activity without metal ions
fructose
5 mM, 107.6% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
glucose
5 mM,24.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
MgCl2
5 mM, 110.1% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 96.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
Mn2+
-
5 min at 50C, 10 mM, 104.1% of the relative activity without metal ions; 5 min at 50C, 1 mM, 105.8% of the relative activity without metal ions
NiCl2
5 mM, 76.7% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 86.5% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
xylose
5 mM, 88% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C
ZnSO4
5 mM, 111.4% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 40C; 5 mM, 90.2% activity compared to activity without addition of MgCl2, 1.25 mM 4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1,2-anhydro-myo-inositol
-
-
Ag+
-
5 min at 50C, 10mM, 100.0% of inhibition; 5 min at 50C, 1 mM, 100.0% inhibition
D-cellobiose
D-glucono-1,5-lactone
-
-
D-glucose
EGTA
-
10% of enzyme inhibition at 20 mM, at 37C, pH 6.5
Fe3+
-
80.5% residual activity at 10 mM
iodoacetoamide
KCl
-
25% of enzyme inhibition at 0.5 mM, at 37C, pH 6.5
Mg2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Mg2+
N-bromosuccinimide
Ni2+
-
the exoglucanase activity is reduced by 5-10% in the presence of 5 mM Ni2+
o-phthaldialdehyde
Thermomonospora sp.
-
-
p-chloromercuribenzoate
-
-
xylobiosyl-deoxynojirimycin
-
X2DNJ
xylobiosyl-isofagomine
-
X2IF
xylobiosyl-isofagomine lactam
-
X2IL
additional information
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ethanol
methanol
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.74
4-nitrophenyl beta-D-cellobioside
-
at 65C in pH 5.5 citrate buffer
0.22 - 2.5
4-nitrophenyl beta-D-glucopyranoside
0.65
alpha-cellulose
-
pH 5.0
-
0.461 - 86
cellobiose
0.028 - 0.73
cellohexaose
0.37 - 6
Cellopentaose
0.77 - 6.7
Cellotetraose
0.44 - 8.7
cellotriose
127
cellulose
-
H3OP4 swollen
10.7
gentiobiose
-
-
0.77
o-nitrophenyl beta-D-glucopyranoside
-
-
0.45
p-nitrophenyl glucoside
-
pH 6.5, at 37C
0.7
p-nitrophenyl-beta-D-cellobioside
2.8 - 4
p-nitrophenyl-beta-D-glucopyranoside
0.42 - 2.2
p-nitrophenyl-beta-D-glucoside
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00039
2,4-dinitrophenol cellobiose
Thermobifida fusca
Q9XCD4
pH 5.5
69.6
4-nitrophenyl beta-D-cellobioside
uncultured bacterium
-
at 65C in pH 5.5 citrate buffer
126.6 - 4945
4-nitrophenyl beta-D-glucopyranoside
0.25 - 281
cellobiose
16.3
cellohexaose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
16.4
Cellopentaose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
18.6
Cellotetraose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
6.21
cellotriose
Fibrobacter succinogenes subsp. succinogenes S85
B0FWI3
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
40.1
4-nitrophenyl beta-D-cellobioside
uncultured bacterium
-
at 65C in pH 5.5 citrate buffer
4692
11900 - 915700
4-nitrophenyl beta-D-glucopyranoside
510
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
29
D-glucose
-
in 100 mM sodium acetate buffer (pH 4.5), at 65C
0.0058 - 0.18
xylobiosyl-deoxynojirimycin
0.00013 - 0.0071
xylobiosyl-isofagomine
0.00034 - 0.024
xylobiosyl-isofagomine lactam
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0011
-
P-42, cotton, pH 5, at 30C
0.0012
-
P-42, avicel, pH 5, at 30C
0.0021
-
P-42, bacterial cellulose, pH 5, at 30C
0.0142
-
Ex-1, cotton, pH 5, at 30C
0.0145
-
Ex-1, bacterial cellulose, pH 5, at 30C
0.0209
-
Ex-1, avicel, pH 5, at 30C
0.0243
-
Ex-1, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30C
0.0244
-
P-42, 4-methylumbelliferyl-beta-D-lactoside, pH 5, at 30C
0.025
-
cellulose MN300
0.026
-
Ex-1, carboxymethyl cellulose, pH 5, at 30C
0.03
-
cellulose HBS
0.0311
-
P-42, carboxymethyl cellulose, pH 5, at 30C
0.04
-
using filter paper as substrate, at 60C in pH 6.5 citrate buffer
0.067
-
crude enzyme from homogenate, using cellobiose as substrate, pH and temperature not specified in the publication
0.1
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
0.102
-
H3PO4 swollen avicel
0.108
-
HCl swollen avicel
0.128
-
celloheptaose
0.228
-
after 3.4fold purification, using cellobiose as substrate, pH and temperature not specified in the publication
0.236
-
cellohexaose
0.323
-
pH 6.5, at 37C
0.35
2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 40C
0.42
-
cellopentaose
0.65
2-nitrophenyl beta-D-galactopyranoside as substrate, pH 4.6, 42C
0.684
-
cellotetraose
0.7
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
2
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
2.1
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
2.53
-
cellulose acetate, pH 5.0
2.6
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
3.1
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
3.2
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
3.3
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
3.36
-
cellulose, pH 5.0
3.4
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
3.583
-
alpha-cellulose, pH 5.0
3.7
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
3.9
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
4
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
5.3
cellobiose as substrate, pH 4.6, 42C
6.8
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
7.9
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 8 weeks
9.4
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
9.5
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
10.7
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
10.9
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
11.5
4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 42C
12.2
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 4 weeks
12.4
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
13.2
Cephalosporium sp.
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
14.5
-
bacterial cellulose powder, not dried, isoenzyme II
14.6
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
15.1
-
bacterial cellulose powder, not dried, isoenzyme I
20.9
-
avicel, isoenzyme II
21.1
-
avicel, isoenzyme I; isoenzyme I
22.6
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 2 weeks
23.3
-
37C, pH 5.0, D-glucose production from cellulose, activity in crude-cell-free fungal extracts collected from fungal cultures on Miscanthus cell walls after 1 week
23.5
-
using phosphate acid swollen cellulose as substrate, at 60C in pH 6.5 citrate buffer
25.5
-
carboxymethyl cellulose, isoenzyme I
27.1
-
p-nitrophenyl beta-D-lactoside, isoenzyme II
27.3
-
p-nitrophenyl beta-D-lactoside, isoenzyme I
33.2
-
p-nitrophenyl beta-D-celloside, isoenzyme II
33.5
cellobiose as substrate, pH 4.6, 40C
34
-
p-nitrophenyl beta-D-celloside, isoenzyme I
41
4-methylumbelliferyl beta-D-cellobioside as substrate, pH 4.6, 40C
52.9
-
using 4-nitrophenyl beta-D-cellobioside as substrate, at 65C in pH 5.5 citrate buffer
58.79
Thermomonospora sp.
-
carboxymethylcellulase activity
77
4-nitrophenyl beta-D-glucopyranoside as substrate, pH 4.6, 42C
129
p-nitrophenyl-beta-D-fucopyranoside as substrate
130
o-nitrophenyl-beta-D-galactopyranoside as substrate; p-nitrophenyl-beta-D-cellobioside as substrate
133
p-nitrophenyl-beta-D-galactopyranoside as substrate
177
p-nitrophenyl-beta-D-glucopyranoside as substrate
220
-
using carboxymethylcellulose as substrate, at 65C in pH 5.5 citrate buffer
693
-
using lichenan as substrate, at 60C in pH 6.5 citrate buffer
717
-
using barley glucan as substrate, at 60C in pH 6.5 citrate buffer
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6
assay at, optimal for 4-nitrophenol beta-D-glucopyranoside as substrate; assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside as substrate
5.5 - 6.5
-
using 4-nitrophenyl beta-D-cellobioside as substrate
6 - 6.5
-
-
7
Thermomonospora sp.
-
for both cellulase and xylanase activity
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 5
-
84% and 86% of the maximum activity appears at pH 4.0 and 5.0, respectively
5 - 9
retaines more than 50% of activity, 50C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37 - 42
-
-
42
assay at, optimal for 4-nitrophenyl beta-D-glucopyranoside as substrate
85
-
avicelase activity
95 - 100
-
carboxymethyl cellulase activity
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 37
-
stable for at least 1 h
40
-
incubation for 3 h, 40% loss of enzyme activity
60 - 70
-
85% and 91% of the maximum activity at 60C and 70C, respectively
70
-
incubation for 3 h, 100% loss of enzyme activity
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.33
Thermomonospora sp.
-
isoelectric focusing
4.9
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
Driselase, from Irpex lacteus
Manually annotated by BRENDA team
Thermomonospora sp.
-
-
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
expressed in Escherichia coli
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
14200
Thermomonospora sp.
-
x * 14200, SDS-PAGE
20000
-
x * 20000, SDS-PAGE
23000
-
23000, SDS-PAGE
29000
-
x * 29000, SDS-PAGE
38400
-
x * 38400, calculated from amino acid sequence
40000
-
x * 40000, SDS-PAGE
42000
-
P-42, SDS-PAGE
47300
-
x * 47300, nonglycosylated form, SDS-PAGE
48600
Sporotrichum pulverulentum
-
ultracentrifugation
49300
-
x * 49300, glycosylated form, SDS-PAGE
56000
-
isoenzyme I
58000
-
x * 58000, SDS-PAGE
62000
-
calculation from sequence of amino acids
64000
-
gel filtration
80000
-
x * 80000, SDS-PAGE
81200
-
x * 81200, SDS-PAGE
83500
-
2 * 83500
85000
-
x * 85000, SDS-PAGE
88000
-
SDS-PAGE
98000
-
8 * 98000, SDS-PAGE
100000
-
alpha,beta, 2 * 100000, SDS-PAGE
112000
-
x * 112000, SDS-PAGE
130300
-
gel filtration
151000
-
gel filtration
169300
-
calculated from amino acid sequence
200000
-
gel filtration
780000
-
gel filtration
additional information
-
70000 g/mol
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
homooctamer
additional information
Thermomonospora sp.
-
fluorescence study on interactions of alpha-crystallin with the molten globule state of the enzyme induced by guanidine hydrochloride
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
no modification
proteolytic modification
-
Papain digestion of native Ex-1, core fragment obtaining
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
resolution 2.0 A
-
combination of the immunoglobulin-like module and the catalytic module and complex of inactive mutant with cellotetraose, modified microbatch crystallization under oil at 0C
-
molecular docking studies for the enzyme with the substrates cellobiose and 4-nitrophenyl-beta-D-glucopyranoside. The essential catalytic residues of RuBG3A are D297 and E499. Besides D113, K218, H219, and R185 also form hydrogen bonds with substrates and are condervative. The active center for beta-glucosidic and beta-xylosidic substrates are the same and the bifunctional activity should come from one functional domain. RuBG3A and RuBG3B exhibit higher scores in the docking analysis against 4-nitrophenyl-beta-D-glucopyranoside than against 4-nitrophenyl-beta-D-xylopyranoside, indicating a larger affinity between the enzymes and 4-nitrophenyl-beta-D-glucopyranoside, which is consistent