Information on EC 3.2.1.72 - xylan 1,3-beta-xylosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.72
-
RECOMMENDED NAME
GeneOntology No.
xylan 1,3-beta-xylosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of successive xylose residues from the non-reducing termini of (1->3)-beta-D-xylans
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(1,3)-beta-D-xylan degradation
-
-
d-xylose degradation
-
-
SYSTEMATIC NAME
IUBMB Comments
3-beta-D-xylan xylohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-50-9
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain A2
-
-
Manually annotated by BRENDA team
strain A2
-
-
Manually annotated by BRENDA team
strain XY-214
Swissprot
Manually annotated by BRENDA team
strain XY-214
Swissprot
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta -1,4-xylotetraose
D-xylose
show the reaction diagram
beta-1,3-xylobiose + H2O
D-xylose
show the reaction diagram
beta-1,3-xylotetraose + H2O
D-xylose
show the reaction diagram
beta-1,3-xylotriose + H2O
D-xylose
show the reaction diagram
beta-1,4-xylobiose
D-xylose
show the reaction diagram
low activity
-
-
-
beta-1,4-xylotriose
D-xylose
show the reaction diagram
p-nitrophenyl-beta-D-xylopyranose + H2O
p-nitrophenol + D-xylopyranose
show the reaction diagram
no activity on laminarioligosaccharides, beta-1,3-linked D-glucose units, beta-1,3-xylan, beta-1,4-xylan, carboxymethyl cellulose, laminaran, p-nitrophenyl-beta-D-xylopyranoside, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-D-mannopyranoside, p-nitrophenyl-beta-Dgalactopyranoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-D-glucopyranoside, p-nitrophenyl-beta-L-fucopyranoside, p-nitrophenyl-beta-L-arabinofuranoside, p-nitrophenyl-beta-L-arabinopyranoside, p-nitrophenyl-beta-D-glucuronide, and p-nitrophenyl-beta-D-cellobioside
-
-
?
beta-1,3-xylan + H2O
additional information
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
beta-1,3-xylan + H2O
additional information
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
more than 90% inhibition
Cu2+
more than 90% inhibition
Hg2+
more than 90% inhibition
Mn2+
more than 90% inhibition
p-chloromercuribenzoic acid
more than 90% inhibition
Pb2+
more than 90% inhibition
Zn2+
more than 90% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.244
p-nitrophenyl-beta-D-xylopyranoside
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
with 2 mM p-nitrophenyl-beta-D-xylopyranoside at 37°C
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 9
retaining more than 80% of the original activity after preincubation in the buffer at various pHs at 4°C for 12 h
677708
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
wild type by DEAE-Toyopearl 650M and MonoQ 5/50 column chromatography, recombinant protein by HiTrap chelating HP column chromatography
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli