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Information on EC 3.2.1.68 - isoamylase

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EC Tree
     3 Hydrolases
         3.2 Glycosylases
             3.2.1 Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
                3.2.1.68 isoamylase
IUBMB Comments
Also readily hydrolyses amylopectin. Differs from EC 3.2.1.41 (pullulanase) and EC 3.2.1.142 (limit dextrinase) by its inability to hydrolyse pullulan, and by limited action on alpha-limit dextrins. Maltose is the smallest sugar it can release from an alpha-(1->6)-linkage.
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This record set is specific for:
UNIPROT: Q8L735
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
isoamylase, glycogen debranching enzyme, starch debranching enzyme, alpha-1,6-glucosidase, glycogen-debranching enzyme, isoamylase1, isoamylase 3, atisa3, atisa1, isoamylase-type debranching enzyme, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
isoamylase-type starch debranching enzyme 2
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debranching enzyme
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
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SYSTEMATIC NAME
IUBMB Comments
glycogen 6-alpha-D-glucanohydrolase
Also readily hydrolyses amylopectin. Differs from EC 3.2.1.41 (pullulanase) and EC 3.2.1.142 (limit dextrinase) by its inability to hydrolyse pullulan, and by limited action on alpha-limit dextrins. Maltose is the smallest sugar it can release from an alpha-(1->6)-linkage.
CAS REGISTRY NUMBER
COMMENTARY hide
9067-73-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene isa2
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ISOA2_ARATH
882
0
98884
Swiss-Prot
-
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant C-terminally His8-tagged single subunit ISA2 or His8-tagged ISA2 in complex with ISA1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene isa2, single expression of C-terminally His8-tagged subunit in Escherichia coli strain BL21(DE3) or co-expression with untagged ISA1 subunit in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wattebled, F.; Dong, Y.; Dumez, S.; Delvalle, D.; Planchot, V.; Berbezy, P.; Vyas, D.; Colonna, P.; Chatterjee, M.; Ball, S.; D'Hulst, C.
Mutants of Arabidopsis lacking a chloroplastic isoamylase accumulate phytoglycogen and an abnormal form of amylopectin
Plant Physiol.
138
184-195
2005
Arabidopsis thaliana (O04196), Arabidopsis thaliana (Q8L735), Arabidopsis thaliana (Q9M0S5)
Manually annotated by BRENDA team
Facon, M.; Lin, Q.; Azzaz, A.M.; Hennen-Bierwagen, T.A.; Myers, A.M.; Putaux, J.L.; Roussel, X.; DHulst, C.; Wattebled, F.
Distinct functional properties of isoamylase-type starch debranching enzymes in monocot and dicot leaves
Plant Physiol.
163
1363-1375
2013
Zea mays, Zea mays (B6U0X5), Arabidopsis thaliana (O04196), Arabidopsis thaliana (Q8L735), Arabidopsis thaliana Col-0 (O04196), Arabidopsis thaliana Col-0 (Q8L735)
Manually annotated by BRENDA team
Sundberg, M.; Pfister, B.; Fulton, D.; Bischof, S.; Delatte, T.; Eicke, S.; Stettler, M.; Smith, S.M.; Streb, S.; Zeeman, S.C.
The heteromultimeric debranching enzyme involved in starch synthesis in Arabidopsis requires both isoamylase1 and isoamylase2 subunits for complex stability and activity
PLoS ONE
8
e75223
2013
Arabidopsis thaliana (O04196), Arabidopsis thaliana (Q8L735)
Manually annotated by BRENDA team