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alpha-limit dextrin + H2O
linear maltooligosaccharides
-
-
-
?
amylopectin + H2O
maltose + maltooligosaccharides
isoamylase is a direct debranching enzyme with the ability to cleave all the alpha-1,6 linkages of glycogen both inner and outer branching points of soluble amylopectin
-
-
?
beta-limit dextrin + H2O
linear maltooligosaccharides
-
-
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
-
-
?
alpha-D-glucan + H2O
?
-
cleavage of alpha-D-glucosidic linkages of certain branched alpha-D-glucans
-
-
?
amylopectin + H2O
?
-
the enzyme can debranch almost all of component chains of amylopectin
-
-
?
amylopectin + H2O
linear maltooligosaccharides
-
-
-
-
?
amylopectin + H2O
maltose + maltooligosaccharides
amylopectin phi-dextrin + H2O
maltotetraose
-
substrate on which all 3 types of debranching enzymes act
-
?
branched chain oligosaccharides + H2O
maltotriose + higher maltosaccharides
-
-
no detectable maltose released, characteristic action is hydrolysis of alpha 1,6-glucosidic linkages, but alpha 1,6 glucosidic linkages attaching maltose residues to chains of alpha 1,4-linked glucose residues are not readily hydrolyzed
?
corn amylopectin + H2O
?
-
-
-
-
?
di-O-alpha-maltosyl-beta-cyclodextrin + H2O
maltose + beta-cyclodextrin
-
-
-
-
r
glycogen + H2O
maltose + maltooligosaccharides
phytoglycogen + H2O
?
-
the enzyme can debranch almost all of component chains of phytoglycogen
-
-
?
additional information
?
-
amylopectin + H2O
maltose + maltooligosaccharides
-
waxy maize amylopectin, potato amylopectin, amylopectin beta-dextrin
-
?
amylopectin + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins, cleaves the branching points completely in vitro
-
?
amylopectin + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins, cleaves the branching points completely in vitro
-
?
amylopectin + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins, cleaves the branching points completely in vitro
-
?
amylopectin + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins, cleaves the branching points completely in vitro
-
?
amylopectin + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins, cleaves the branching points completely in vitro
-
?
amylopectin + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins, cleaves the branching points completely in vitro
-
-
?
amylopectin + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins, cleaves the branching points completely in vitro
hydrolysis of beta-limit dextrins forming maltotriose as main reaction product
?
amylopectin + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins, cleaves the branching points completely in vitro
hydrolysis of beta-limit dextrins forming maltotriose as main reaction product
?
glycogen + H2O
maltose + maltooligosaccharides
-
cleaves the branching points completely in vitro
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
cleaves the branching points completely in vitro
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
cleaves the branching points completely in vitro
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
cleaves the branching points completely in vitro
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
cleaves the branching points completely in vitro
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
cleaves the branching points completely in vitro
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
cleaves the branching points completely in vitro
-
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
oyster glycogen, glycogen beta-dextrin, rabbit liver glycogen
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins
-
?
glycogen + H2O
maltose + maltooligosaccharides
-
or the beta-limit dextrins
-
-
?
maltose + H2O
?
-
-
-
-
?
maltose + H2O
?
-
iam gene induced by maltose
-
-
?
additional information
?
-
no activity towards pullulan
-
-
?
additional information
?
-
-
direct debranching enzyme, attacks unmodified glycogen and/or amylopectin with hydrolysis of the 1,6-bond, smallest substrate is not known
-
-
?
additional information
?
-
-
direct debranching enzyme, attacks unmodified glycogen and/or amylopectin with hydrolysis of the 1,6-bond, smallest substrate is not known
-
-
?
additional information
?
-
-
distinguished from alpha-dextrin endo-1,6-alpha-glucosidase, EC 3.2.1.41, by the inability of isoamylase to attack pullulan, and by limited action on alpha-limit dextrins, action on glycogen however is complete in contrast to limited action by alpha-dextrin glucanohydrolase, 1,6-linkage hydrolysed only at branch point
-
-
?
additional information
?
-
-
isoamylase is one of the starch-debranching enzymes which catalyzes the hydrolysis of alpha-1,6-glucosidic linkages specific in alpha-glucans including amylopectin, branched starch, and glycogen
-
-
?
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Amemura, A.; Chakraborty, R.; Fujita, M.; Noumi, T.; Masamitsu, F.
Cloning and nucleotide sequence of the isoamylase gene from Pseudomonas amyloderamosa SB-15
J. Biol. Chem.
263
9271-9275
1988
Pseudomonas amyloderamosa
brenda
Amemura, A.; Konishi, Y.; Harada, T.
Molecular weight of the undegraded polypeptide chain of Pseudomonas amyloderamosa isoamylase
Biochim. Biophys. Acta
611
390-393
1980
Pseudomonas amyloderamosa
brenda
Kato, K.; Konishi, Y.; Amemura, A.; Harada, T.
Affinity chromatography of Pseudomonas isoamylase on cross-linked amylose gel
Agric. Biol. Chem.
41
2077-2080
1977
Pseudomonas amyloderamosa
-
brenda
Kitagawa, H.; Amemura, A.; Harada, T.
Studies on the inhibition and molecular properties of crystalline Pseudomonas isoamylase
Agric. Biol. Chem.
39
989-994
1975
Pseudomonas amyloderamosa
-
brenda
Lee, E.Y.C.; Whelan, W.J.
Glycogen and starch debranching enzymes
The Enzymes, 3rd Ed. (Boyer, P. D. , ed. )
5
191-234
1972
Cytophaga sp., Pseudomonas amyloderamosa
-
brenda
Yokobayashi, K.; Misaki, A.; Harada, T.
Purification and properties of Pseudomonas isoamylase
Biochim. Biophys. Acta
212
458-469
1970
Pseudomonas amyloderamosa
brenda
Fujita, F.; Sakai, S.; Futai, M.; Amemura, A.
Characterization of an isoamylase-hyperproducing mutant of Pseudomonas amyloderamosa
Agric. Biol. Chem.
54
2315-2321
1990
Pseudomonas amyloderamosa, Pseudomonas amyloderamosa MI-414
-
brenda
Katsuya, Y.; Mezaki, Y.; Kubota, M.; Matsuura, Y.
Three-dimensional structure of Pseudomonas isoamylase at 2.2 A resolution
J. Mol. Biol.
281
885-897
1998
Pseudomonas amyloderamosa
brenda
Ara, K.; Saeki, K.; Ito, S.
Purification and characterization of an alkaline isomylase from an alkalophilic strain of Bacillus
J. Gen. Microbiol.
139
781-786
1993
Bacillus sp. (in: Bacteria), Pseudomonas amyloderamosa, Pseudomonas amyloderamosa SMP1
-
brenda
Lin, L.L.; Fang, T.Y.; Chu, W.S.; Hsu, W.H.
Improved elution of isoamylase adsorbed on raw starch and the preservation of purified enzyme
Lett. Appl. Microbiol.
19
383-385
1994
Pseudomonas amyloderamosa, Pseudomonas amyloderamosa WU-5315
-
brenda
Krohn, M.; Barry, G.F.; Kishore, G.M.
An isoamylase with neutral pH optimum from a Flavobacterium species: cloning, characterization and expression of the iam gene
Mol. Gen. Genet.
254
469-478
1997
Flavobacterium sp., Pseudomonas amyloderamosa, Pseudomonas amyloderamosa JD210, Pseudomonas amyloderamosa SMP1
brenda
Yoon, S.; Robyt, J.F.
Activation and stabilization of 10 starch-degrading enzymes by Triton X-100, polyethylene glycols, and polyvinyl alcohols
Enzyme Microb. Technol.
37
556-562
2005
Pseudomonas amyloderamosa
-
brenda
Kang, H.K.; Cha, H.; Yang, T.J.; Park, J.T.; Lee, S.; Kim, Y.W.; Auh, J.H.; Okada, Y.; Kim, J.W.; Cha, J.; Kim, C.H.; Park, K.H.
Enzymatic synthesis of dimaltosyl-beta-cyclodextrin via a transglycosylation reaction using TreX, a Sulfolobus solfataricus P2 debranching enzyme
Biochem. Biophys. Res. Commun.
366
98-103
2008
Pseudomonas amyloderamosa
brenda
Park, I.M.; Ibanez, A.M.; Shoemaker, C.F.
Rice starch molecular size and its relationship with amylose content
Starch Staerke
59
69-77
2007
Pseudomonas amyloderamosa
-
brenda
Wang, S.; Lai, J.; Huang, M.; Tai, C.; Liu, H.
Deactivation of isoamylase and β-amylase in the agitated reactor under supercritical carbon dioxide
Bioprocess Biosyst. Eng.
33
1007-1015
2010
Pseudomonas amyloderamosa, Pseudomonas amyloderamosa WU7211-2
brenda
Ray, R.
Microbial isoamylases: An overview
Am. J. Food Technol.
6
1-18
2011
Myroides odoratus, Pseudomonas amyloderamosa (P10342), Pseudomonas amyloderamosa SB-15 (P10342)
-
brenda
Kobayashi, T.; Sasaki, S.; Utsumi, Y.; Fujita, N.; Umeda, K.; Sawada, T.; Kubo, A.; Abe, J.; Colleoni, C.; Ball, S.; Nakamura, Y.
Comparison of chain-length preferences and glucan specificities of isoamylase-type alpha-glucan debranching enzymes from rice, Cyanobacteria, and Bacteria
PLoS ONE
11
e0157020
2016
Crocosphaera subtropica ATCC 51142, Escherichia coli, Oryza sativa, Pseudomonas amyloderamosa, Synechococcus elongatus, Synechococcus elongatus PCC7942
brenda