Information on EC 3.2.1.64 - 2,6-beta-fructan 6-levanbiohydrolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.64
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RECOMMENDED NAME
GeneOntology No.
2,6-beta-fructan 6-levanbiohydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i.e. 6-(beta-D-fructofuranosyl)-D-fructose, from the end of the chain
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
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SYSTEMATIC NAME
IUBMB Comments
(2->6)-beta-D-fructofuranan 6-(beta-D-fructosyl)-D-fructose-hydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-46-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC 15953
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Manually annotated by BRENDA team
strain 11
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-
Manually annotated by BRENDA team
strain 11
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-fructosylnystose + H2O
beta-D-fructose + alpha-D-glucose + sucrose + 1-kestose + nystose
show the reaction diagram
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at 81.4% the rate of levan
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?
1-kestose + H2O
?
show the reaction diagram
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0.2% of the activity with levan from Serratia sp.
-
?
1-kestose + H2O
sucrose + beta-D-fructose + alpha-D-glucose
show the reaction diagram
3 levan + 7 H2O
10 levanbiose
show the reaction diagram
inulin + H2O
?
show the reaction diagram
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5% of the activity with levan from Serratia sp.
-
?
levan + H2O
levanbiose + ?
show the reaction diagram
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enzyme activity depends on source of levan, sources in decreasing order of activity: Serratia sp., Rhanella aquatilis, Zymomonas mobilis, M. laevaniformans
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?
nystose + H2O
?
show the reaction diagram
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0.5% of the activity with levan from Serratia sp.
-
?
nystose + H2O
beta-D-fructose + alpha-D-glucose + sucrose + 1-kestose
show the reaction diagram
sucrose + H2O
beta-D-fructose + alpha-D-glucose
show the reaction diagram
additional information
?
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enzyme cleaves beta-2,6-linkage of levan and beta-2,1-linkage of fructooligosaccharides, no substrate are: soluble starch, cellobiose, sucrose, maltose, melibiose, raffinose, lactose
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3 levan + 7 H2O
10 levanbiose
show the reaction diagram
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
997.3
-
pH 6.0, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 7
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more than 80% of maximum activity in this range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
54000
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1 * 54000, SDS-PAGE
60000
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gel filtration
68800
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x * 68800, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 68800, SDS-PAGE
monomer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 8
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-
136755
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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30 min, 10% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant protein, expression n Escherichia coli
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE