Information on EC 3.2.1.61 - mycodextranase

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The expected taxonomic range for this enzyme is: Bacteria, Eukaryota

EC NUMBER
COMMENTARY hide
3.2.1.61
-
RECOMMENDED NAME
GeneOntology No.
mycodextranase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in alpha-D-glucans containing both (1->3)- and (1->4)-bonds
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(1->3)-(1->4)-alpha-D-glucan 4-glucanohydrolase
Products are nigerose and 4-alpha-D-nigerosylglucose. No hydrolysis of alpha-D-glucans containing only 1,3- or 1,4-bonds.
CAS REGISTRY NUMBER
COMMENTARY hide
9047-04-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
NHB-1
-
-
Manually annotated by BRENDA team
NHB-1
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
IFO 3355
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
nigeran + H2O
nigerose + 4-alpha-D-nigerosylglucose
show the reaction diagram
nigeran + H2O
nigerose + alpha-D-glucopyranosyl-1,3-alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,3-D-glucose
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
nigeran + H2O
nigerose + 4-alpha-D-nigerosylglucose
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ag+
-
fast enzyme
maltotriose
-
-
N-bromosuccinimide
N-Chlorosuccinimide
nigeran tetrasaccharide
-
-
nigeran trisaccharide
-
-
p-chloromercuribenzoic acid
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2 - 9
-
fast and slow enzyme pH 2 15%, pH 9 75% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
-
fast and slow enzyme
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
23 - 80
-
23C: fast enzyme 7%, slow enzyme 16% of maximal activity, 80C: fast enzyme 50%, slow enzyme 30% of maximal activity
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40000
-
sedimentation equilibrium fast and slow enzyme
56078
x * 56078, calculation from nucleotide sequence
68000
-
1 * 68000, SDS-PAGE
120000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
additional information
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 5
-
stable between
136743
5 - 10
-
80% of maximal activity after incubation at 4C for 48 h
136748
6 - 7
-
stable between
136748
6 - 8
-
stable between
136747
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
enzyme stable up to 50C
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
4C, 2 months, no loss of activity, fast and slow enzyme
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
fast and slow enzyme, different electrophoretic mobilities
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis