Information on EC 3.2.1.56 - glucuronosyl-disulfoglucosamine glucuronidase

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The expected taxonomic range for this enzyme is: Pedobacter heparinus

EC NUMBER
COMMENTARY hide
3.2.1.56
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RECOMMENDED NAME
GeneOntology No.
glucuronosyl-disulfoglucosamine glucuronidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-D-glucuronosyl-N2,6-disulfo-beta-D-glucosamine + H2O = D-glucuronate + N2,6-disulfo-D-glucosamine
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
heparan sulfate degradation
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heparin degradation
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SYSTEMATIC NAME
IUBMB Comments
3-D-glucuronsyl-N2,6-disulfo-beta-D-glucosamine glucuronohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
37288-42-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1,3-(4-deoxy-alpha-L-threo-4-enopyranosyluronicacid)-(2-N-acetylgalactosamine-6-O-sulfate) + ?
?
show the reaction diagram
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-
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-
?
disaccharide + SO42-
glucosamine 2,6-disulfate + uronic acid
show the reaction diagram
glucosamine 2,6-disulfate
glucosamine-N-sulfate + SO42-
show the reaction diagram
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-
-
?
heparan sulfate + H2O
?
show the reaction diagram
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unusual glycuronidase, hydrolyses all disaccharides obtained from heparan sulfate
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-
?
heparin + H2O
trisulfated disaccharide + disulfated disaccharide + glucosamine -2,6-disulfate + glucosamine-N-SO4 + SO42-
show the reaction diagram
O-3-D-glucuronosyl-N2,O6-disulfo-beta-D-glucosamine + H2O
N2,O6-disulfo-D-glucosamine + D-glucuronate
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
163
1,3-(4-deoxy-alpha-L-threo-4-enopyranosyluronicacid)-(2-N-acetylgalactosamine-6-O-sulfate)
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
29.4
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gel filtration
50
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enzyme used in solution
259
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crude, enzymatic activity measured by the disappearance of substrate
additional information
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6.470 mg/h
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30
-
relatively temperature insensitive
37
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activity decrease of 50% after 3.2 h
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41800
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SDS-PAGE
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
not highly purified, but 25fold over the preparation described before
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purified by 639fold to apparent homogeneity
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