Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.2.1.52 - beta-N-acetylhexosaminidase and Organism(s) Mus musculus and UniProt Accession Q3U4H6

for references in articles please use BRENDA:EC3.2.1.52
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
Acts on N-acetylglucosides and N-acetylgalactosides.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Mus musculus
UNIPROT: Q3U4H6
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
beta-hexosaminidase, hexosaminidase, n-acetyl-beta-glucosaminidase, o-glcnacase, hex a, n-acetylglucosaminidase, hexosaminidase a, beta-n-acetylhexosaminidase, hex b, beta-hexosaminidase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexosaminidase D
-
2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase
-
-
-
-
65 kDa epididymal boar protein
-
-
-
-
beta-acetylaminodeoxyhexosidase
-
-
-
-
beta-acetylhexosaminidase
-
-
-
-
beta-acetylhexosaminidinase
-
-
-
-
beta-D-hexosaminidase
-
-
-
-
beta-D-N-acetylhexosaminidase
-
-
-
-
Beta-GlcNAcase
-
-
-
-
beta-hexosaminidase
beta-N-acetyl-D-hexosaminidase
-
-
-
-
beta-N-acetyl-hexosaminidase
-
-
-
-
beta-N-acetylgalactosaminidase
-
-
-
-
beta-N-acetylglucosaminidase
-
-
-
-
Beta-N-acetylhexosaminidase
-
-
-
-
beta-NAHA
-
-
-
-
Beta-NAHASE
-
-
-
-
chitobiase
-
-
-
-
Hex
-
-
-
-
hexosaminidase
-
-
-
-
hexosaminidase A
hexosaminidase B
-
-
N-acetyl-beta-D-hexosaminidase
-
-
-
-
N-acetyl-beta-glucosaminidase
-
-
-
-
N-acetyl-beta-hexosaminidase
-
-
-
-
N-acetylhexosaminidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
O-glycosyl bond hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-N-acetyl-D-hexosaminide N-acetylhexosaminohydrolase
Acts on N-acetylglucosides and N-acetylgalactosides.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-33-3
c.f. EC 3.2.1.165
9027-52-5
deleted
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-galactosamine
show the reaction diagram
-
-
-
?
p-nitrophenyl-N-acetyl-D-glucosamine + H2O
N-acetyl-D-glucosamine + p-nitrophenol
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
HEXO2 Is important for limiting growth of Mycobacterium marinum in S2 cells. HEXO2 isoform is sufficient to control bacterial growth in S2 cells
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
-
HEXO2 Is important for limiting growth of Mycobacterium marinum in S2 cells. HEXO2 isoform is sufficient to control bacterial growth in S2 cells
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-acetylcastanospermine
30% with 1 mM inhibitor and 2.5 mM substrate
O-(2-acetamido-2-deoxy-D-glucopyranosylidene)amino N-phenylcarbamate
60% inhibition with 1 mM inhibitor and 2.5 mM substrate
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.25
4-nitrophenyl beta-N-acetyl-D-galactosaminide
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
the importance of beta-hexosaminidase for restricting mycobacterial growth during mammalian infections is confirmed in macrophages from beta-hexosaminidase knockout mice. beta-Hexosaminidase is characterized as a peptidoglycan hydrolase that exerts its mycobactericidal effect at the macrophage plasma membrane during mycobacteria-induced secretion of lysosomes
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HEXD_MOUSE
486
0
54576
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000 - 62000
Western blot analysis, shorter and longer form
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
gel filtration
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3)pLysS cells. Plasmid encoding an N-terminally Myc-tagged form of the full-length murine hexosaminidase transfected into murine NIH 3T3 fibroblast cells. Myc-tagged protein transfected into COS cells
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
therapeutic approach for Sandhoff disease
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Arfi, A.; Zisling, R.; Richard, E.; Batista, L.; Poenaru, L.; Futerman, A.H.; Caillaud, C.
Reversion of the biochemical defects in murine embryonic Sandhoff neurons using a bicistronic lentiviral vector encoding hexosaminidase alpha and beta
J. Neurochem.
96
1572-1579
2006
Mus musculus
Manually annotated by BRENDA team
Koo, I.C.; Ohol, Y.M.; Wu, P.; Morisaki, J.H.; Cox, J.S.; Brown, E.J.
Role for lysosomal enzyme beta-hexosaminidase in the control of mycobacteria infection
Proc. Natl. Acad. Sci. USA
105
710-715
2008
Drosophila melanogaster, Mus musculus
Manually annotated by BRENDA team
Gutternigg, M.; Rendic, D.; Voglauer, R.; Iskratsch, T.; Wilson, I.B.
Mammalian cells contain a second nucleocytoplasmic hexosaminidase
Biochem. J.
419
83-90
2009
Homo sapiens, Mus musculus (Q3U4H6), Mus musculus
Manually annotated by BRENDA team