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Information on EC 3.2.1.52 - beta-N-acetylhexosaminidase and Organism(s) Bos taurus and UniProt Accession Q0V8R6

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EC Tree
IUBMB Comments
Acts on N-acetylglucosides and N-acetylgalactosides.
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Select one or more organisms in this record: ?
This record set is specific for:
Bos taurus
UNIPROT: Q0V8R6
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Word Map
The taxonomic range for the selected organisms is: Bos taurus
The enzyme appears in selected viruses and cellular organisms
Synonyms
beta-hexosaminidase, hexosaminidase, n-acetyl-beta-glucosaminidase, o-glcnacase, hex a, n-acetylglucosaminidase, hexosaminidase a, beta-n-acetylhexosaminidase, hex b, beta-hexosaminidase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
beta-N-acetylglucosaminidase
-
neutral hexosaminidase C
-
O-GlcNAcase
-
2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase
-
-
-
-
65 kDa epididymal boar protein
-
-
-
-
beta-acetylaminodeoxyhexosidase
-
-
-
-
beta-acetylhexosaminidase
-
-
-
-
beta-acetylhexosaminidinase
-
-
-
-
beta-D-hexosaminidase
-
-
-
-
beta-D-N-acetylhexosaminidase
-
-
-
-
Beta-GlcNAcase
-
-
-
-
beta-hexosaminidase
-
-
-
-
beta-N-acetyl-D-hexosaminidase
-
-
-
-
beta-N-acetyl-hexosaminidase
-
-
-
-
beta-N-acetylgalactosaminidase
-
-
-
-
beta-N-acetylglucosaminidase
-
-
-
-
Beta-N-acetylhexosaminidase
-
-
-
-
beta-NAHA
-
-
-
-
Beta-NAHASE
-
-
-
-
chitobiase
-
-
-
-
Hex
-
-
-
-
hexosaminidase
-
-
-
-
hexosaminidase A
-
-
-
-
N-acetyl-beta-D-hexosaminidase
-
-
-
-
N-acetyl-beta-glucosaminidase
-
-
-
-
N-acetyl-beta-hexosaminidase
-
-
-
-
N-acetylhexosaminidase
-
-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
O-glycosyl bond hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-N-acetyl-D-hexosaminide N-acetylhexosaminohydrolase
Acts on N-acetylglucosides and N-acetylgalactosides.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-33-3
c.f. EC 3.2.1.165
9027-52-5
deleted
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
show the reaction diagram
-
-
-
?
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
show the reaction diagram
-
-
-
-
?
alpha1-acid glycoprotein + H2O
?
show the reaction diagram
-
-
-
-
?
chitin oligosaccharides + H2O
N-acetyl-D-glucosamine
show the reaction diagram
-
(N-acetyl-D-glucosamine)2-4
-
-
?
chitobiose + H2O
2 D-glucosamine
show the reaction diagram
-
-
-
?
glycopeptides derived from asialo ovalbumin + H2O
?
show the reaction diagram
-
hydrolyzes terminal and nonreducing residues, no hydrolysis of the intact glycoprotein
-
-
?
glycopeptides derived from asialo ovomucoid + H2O
?
show the reaction diagram
-
hydrolyzes terminal and nonreducing residues, no hydrolysis of the intact glycoprotein
-
-
?
kappa-casein glycopeptide + H2O
?
show the reaction diagram
-
-
-
-
?
lacto-N-triose + H2O
?
show the reaction diagram
-
-
-
-
?
methyl-beta-N-acetyl-glucosaminide + H2O
methanol + beta-N-acetyl-glucosamine
show the reaction diagram
-
-
-
-
?
N,N'-diacetylchitobiose + H2O
N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
-
?
N-acetyl-beta-D-glucosaminide-1,4-muramic acid + H2O
?
show the reaction diagram
-
-
-
-
?
N-acetyl-beta-D-hexosaminides + H2O
N-acetyl-D-hexosamines
show the reaction diagram
ovalbumin glycopeptides + H2O
?
show the reaction diagram
-
-
-
-
?
ovomucoid glycopeptide + H2O
?
show the reaction diagram
-
-
-
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
2-acetamido-2-deoxy-D-galactose + p-nitrophenol
show the reaction diagram
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
2-acetamido-2-deoxy-D-glucose + p-nitrophenol
show the reaction diagram
phenyl-N-acetyl-beta-D-glucosaminide + H2O
phenol + N-acetyl-beta-D-glucosaminide
show the reaction diagram
-
-
-
-
?
tri-N-acetylchitotriose + H2O
N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
-
?
trihexosylceramide + H2O
?
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-acetyl-beta-D-hexosaminides + H2O
N-acetyl-D-hexosamines
show the reaction diagram
additional information
?
-
-
N-acetyl-beta-hexosaminidases catalyze the hydrolysis of nonreducing terminal N-acetyl-beta-D-glucosamine and N-acetyl-beta-D-galactosamine units in glycoproteins, GM2-gangliosides and glycosaminoglycans including chondroitin 4-sulfate, chondroitin 6-sulfate, hyaluronic acid, keratan sulfate and dermatan sulfate
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
N-acetyl-beta-D-glucosamine
inhibited by GlcNAc, but not by GalNAc
(3R,4R,5R,6S),N-(4,5,6-trihydroxyazepan-3-yl)-acetamide
-
68% inhibition at 1 mM
(3R,4S,5R,6R,7R),N-(3,5,6-trihydroxy-7-hydroxymethyl-azepan-4-yl)-acetamide
-
99% inhibition at 1 mM
(3S,4R,5R,6R,7R),N-(3,5,6-trihydroxy-7-hydroxymethyl-azepan-4-yl)-acetamide
-
93% inhibition at 1 mM
(3S,4R,5R,6R,7R),N-(4,5,6-trihydroxy-7-hydroxymethyl-azepan-3-yl)-acetamide
-
74% inhibition at 1 mM
(3S,4R,5R,6R,7S),N-(3,5,6-trihydroxy-7-hydroxymethyl-azepan-4-yl)-acetamide
-
99% inhibition at 1 mM
(3S,4R,5R,6S),N-(4,5,6-trihydroxyazepan-3-yl)-acetamide
-
99% inhibition at 1 mM
2-acetamido-1,2,4-trideoxy-1,4-imino-D-arabinitol
-
poor inhibitor
2-acetamido-1,2,4-trideoxy-1,4-imino-L-arabinitol
-
-
2-acetamido-2-deoxy-D-galactono(1-4)lactone
-
-
2-acetamido-2-deoxy-D-mannono-1,4-lactone
-
-
2-acetamido-2-deoxyglucono-1,4-lactone
-
-
acetate
EDTA
-
with 50 mM 54% inhibition
Fe2+
-
-
Fe3+
-
-
galactonolactone
-
-
galactosamine
gluconolactone
-
-
glucosamine
iodoacetamide
-
-
N-(3R,4S,5R,6R,7S)-(3,5,6-trihydroxy-7-hydroxymethyl-azepan-4-yl)-acetamide
-
40% inhibition at 1 mM
N-acetyl-D-galactosamine
N-acetyl-D-glucosamine
N-benzyl-2-acetamido-1,2,4-trideoxy-1,4-imino-D-arabinitol
-
potent inhibitor
N-benzyl-2-acetamido-1,2,4-trideoxy-1,4-imino-L-arabinitol
-
-
N-ethylmaleimide
-
N-acetylglucosaminidase
N-[(3S,4R,5R,6S)-4,5,6-trihydroxyazepan-3-yl]acetamide
-
-
p-chloromercuribenzoate
-
-
p-hydroxymercuribenzoate
-
inhibits N-acetylhexosaminidase, -galactosaminidase and -glucosaminidase
Sodium acetate
-
with 50 mM 27% inhibition
additional information
-
design and synthesis of acetamido tri- and tetra-hydroxyazepanes as potent and selective beta-N-acetylhexosaminidase inhibitors
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
bovine serum albumin
-
-
-
cysteine
-
-
N-ethylmaleimide
-
N-acetylgalactosaminidase
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.08 - 0.54
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
0.32 - 2.38
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0006
(3R,4S,5R,6R,7R),N-(3,5,6-trihydroxy-7-hydroxymethyl-azepan-4-yl)-acetamide
-
-
0.026
(3S,4R,5R,6R,7R),N-(3,5,6-trihydroxy-7-hydroxymethyl-azepan-4-yl)-acetamide
-
-
0.0004
(3S,4R,5R,6R,7S),N-(3,5,6-trihydroxy-7-hydroxymethyl-azepan-4-yl)-acetamide
-
-
0.104
2-acetamido-1,2,4-trideoxy-1,4-imino-D-arabinitol
-
-
0.000095
2-acetamido-1,2,4-trideoxy-1,4-imino-L-arabinitol
-
-
0.0169
N-benzyl-2-acetamido-1,2,4-trideoxy-1,4-imino-D-arabinitol
-
-
0.00028
N-benzyl-2-acetamido-1,2,4-trideoxy-1,4-imino-L-arabinitol
-
-
0.0007
N-[(3S,4R,5R,6S)-4,5,6-trihydroxyazepan-3-yl]acetamide
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.84
purified protein
0.03
-
N-acetylhexosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
0.22
-
N-acetylhexosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
0.63
-
-
1.43
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form A
18.1
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form B
2.08
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form B
21.1
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, form A
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.4
-
N-acetylgalactosamnidase activity
3.8
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside, N-acetylhexosaminidase
4.2
-
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside, N-acetylhexosaminidase
4.5
-
N-acetylglucosamnidase activity
5.2
-
N-acetylglucosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
5.5
-
N-acetylgalactosaminidase, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
mostly localized
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
-
in milk of cows with mastitis of the mammary gland high concentrations of enzymes are found
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HEXA_BOVIN
529
0
60353
Swiss-Prot
Secretory Pathway (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
103000
SDS-PAGE
118000
-
A form, gel filtration
13400
-
1 * 53000 + 1 * 13400, SDS-PAGE
140000
-
sedimentation equilibrium
190000
-
gel filtration
220000
-
B form, sedimentation equilibrium analysis
234000
-
B form, gel filtration
25000
-
A and B form: 1 * 55000 + 1 * 25000, SDS-PAGE
53000
-
1 * 53000 + 1 * 13400, SDS-PAGE
55000
-
A and B form: 1 * 55000 + 1 * 25000, SDS-PAGE
61000
-
SDS-PAGE
62500
-
gel filtration
additional information
-
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
additional information
-
-
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
at 37°C, stable for 15 min
393725
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
no loss of activity on repeated freezing and thawing
-
unstable during chromatography and dialysis
-
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-mercaptoethanol
-
denaturation in subunits
dithiothreitol
-
2 mM cause total inhibition
guanidine-HCl
-
denaturation in subunits
urea
-
denaturation in subunits
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme can be stored at -20°C
-20°C, crude extract
-
-20°C, N-acetyl-glucosaminidase and -galactosaminidase completely lose activity after several days
-
-20°C, N-acetyl-hexosaminidase, in presence of ammonium sulfate, after several weeks: precipitate devoid of enzymatic activity forms but can be removed by centrifugation, further purification: loss of activity after several days at -20°C
-
-20°C, stable for at least 1 year
-
2°C, in (NH4)2SO4, stable
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
135fold to homogeneity
-
310fold
-
A form, still heterogenous: 63fold, B form, close to homogeneity: 92fold
-
A form: 310fold, B form: 260fold, to homogeneity
-
N-acetylhexosaminidase: 63fold, N-acetylglucosaminidase and -galactosaminidase
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
-
veterinary medicine: increased level of enzyme in secreted milk can be detected by diagnosis test for mastitis of the mammary gland of cows
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Frohwein, Y.Z.; Gatt, S.
Isolation of beta-N-acetylhexosaminidase, beta-N-acetylglucosaminidase, and beta-N-acetylgalactosaminidase from calf brain
Biochemistry
6
2775-2782
1967
Bos taurus, Rattus norvegicus
Manually annotated by BRENDA team
Sarber, R.L.; Distler, J.J.; Jourdian, G.W.
beta-N-Acetylhexosaminidase from bovine testes
Methods Enzymol.
50
520-523
1978
Bos taurus
Manually annotated by BRENDA team
Pokorny, M.; Glaudemans, P.J.
Purification of N-acetyl beta-D-hexosaminidase from bull epididymis by affinity chromatography
FEBS Lett.
50
66-69
1975
Bos taurus
Manually annotated by BRENDA team
Kitchen, B.J.; Masters, C.J.
Purification and properties of bovine mammary gland N-acetyl-beta-D-glucosaminidase
Biochim. Biophys. Acta
831
125-132
1985
Bos taurus
Manually annotated by BRENDA team
Khar, A.; Anand, S.R.
Studies on the glycosidases of semen. Purification and properties of beta-N-acetylglucosaminidase from bull sperm
Biochim. Biophys. Acta
483
141-151
1977
Bos taurus
Manually annotated by BRENDA team
Verpoorte, J.A.
Purification of two beta-N-acetyl-D-glucosaminidases from beef spleen
J. Biol. Chem.
247
4787-4793
1972
Bos taurus
Manually annotated by BRENDA team
Rountree, J.S.; Butters, T.D.; Wormald, M.R.; Boomkamp, S.D.; Dwek, R.A.; Asano, N.; Ikeda, K.; Evinson, E.L.; Nash, R.J.; Fleet, G.W.
Design, synthesis, and biological evaluation of enantiomeric beta-N-acetylhexosaminidase inhibitors LABNAc and DABNAc as potential agents against Tay-Sachs and Sandhoff disease
ChemMedChem
4
378-392
2009
Bos taurus, Homo sapiens, Canavalia sp.
Manually annotated by BRENDA team
Gao, Y.; Wells, L.; Comer, F.I.; Parker, G.J.; Hart, G.W.
Dynamic O-glycosylation of nuclear and cytosolic proteins: cloning and characterization of a neutral, cytosolic beta-N-acetylglucosaminidase from human brain
J. Biol. Chem.
276
9838-9845
2001
Bos taurus (Q0V8R6)
Manually annotated by BRENDA team
Li, H.; Marcelo, F.; Bello, C.; Vogel, P.; Butters, T.D.; Rauter, A.P.; Zhang, Y.; Sollogoub, M.; Bleriot, Y.
Design and synthesis of acetamido tri- and tetra-hydroxyazepanes: potent and selective beta-N-acetylhexosaminidase inhibitors
Bioorg. Med. Chem.
17
5598-5604
2009
Bos taurus, Canavalia sp.
Manually annotated by BRENDA team