Any feedback?
Information on EC 3.2.1.52 - beta-N-acetylhexosaminidase and Organism(s) Ostrinia furnacalis and UniProt Accession Q06GJ0
for references in articles please use BRENDA:EC3.2.1.52Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
3.2.1.52 beta-N-acetylhexosaminidaseIUBMB Comments
Acts on N-acetylglucosides and N-acetylgalactosides.
Specify your search results
Word Map
- 3.2.1.52
- urinary
- tay-sachs
- gm2
- beta-galactosidase
- beta-glucuronidase
- urine
- n-acetylglucosamine
-
mast
- isoenzymes
-
o-glcnacylation
- hydrolases
- ganglioside
- sandhoff
- creatinine
-
o-linked
- oligosaccharide
- tubular
-
degranulation
- alpha-mannosidase
- gangliosidosis
- glycosidases
- ige
- cathepsins
-
infantile
- beta-glucosidase
-
rbl-2h3
-
n-acetyl-beta-d-glucosaminidase
- histamine
-
nephrotoxicity
-
hexosamine
-
basophil
- alpha-galactosidase
- arylsulfatase
- chitinases
- alpha-fucosidase
-
chitinolytic
-
jewish
-
ashkenazi
-
exoglycosidases
-
ige-mediated
- n-acetylgalactosamine
-
2-microglobulin
- fcepsilonri
- endochitinase
-
4-methylumbelliferyl
-
3.2.1.23
- mucolipidosis
-
anti-allergic
- arylamidase
- chitobiose
- drug development
- diagnostics
- medicine
- food industry
- analysis
The taxonomic range for the selected organisms is: Ostrinia furnacalis
The enzyme appears in selected viruses and cellular organisms
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
Synonyms
beta-hexosaminidase, hexosaminidase, n-acetyl-beta-glucosaminidase, o-glcnacase, hex a, n-acetylglucosaminidase, hexosaminidase a, beta-n-acetylhexosaminidase, hex b, beta-hexosaminidase a, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase
-
-
-
-
65 kDa epididymal boar protein
-
-
-
-
beta-acetylaminodeoxyhexosidase
-
-
-
-
beta-acetylhexosaminidase
-
-
-
-
beta-acetylhexosaminidinase
-
-
-
-
beta-D-hexosaminidase
-
-
-
-
beta-D-N-acetylhexosaminidase
-
-
-
-
Beta-GlcNAcase
-
-
-
-
beta-hexosaminidase
-
-
-
-
beta-N-acetyl-D-hexosaminidase
-
-
-
-
beta-N-acetyl-hexosaminidase
-
-
-
-
beta-N-acetylgalactosaminidase
-
-
-
-
beta-N-acetylglucosaminidase
-
-
-
-
Beta-N-acetylhexosaminidase
-
-
-
-
beta-NAHA
-
-
-
-
Beta-NAHASE
-
-
-
-
chitobiase
-
-
-
-
Hex
-
-
-
-
hexosaminidase
-
-
-
-
hexosaminidase A
-
-
-
-
N-acetyl-beta-D-hexosaminidase
N-acetyl-beta-glucosaminidase
-
-
-
-
N-acetyl-beta-hexosaminidase
-
-
-
-
N-acetylhexosaminidase
-
-
-
-
N-acetyl-beta-D-hexosaminidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
O-glycosyl bond hydrolysis
-
-
-
-
PATHWAY SOURCE
PATHWAYS
KEGG
-
-, -, -, -
SYSTEMATIC NAME
IUBMB Comments
beta-N-acetyl-D-hexosaminide N-acetylhexosaminohydrolase
Acts on N-acetylglucosides and N-acetylgalactosides.
CAS REGISTRY NUMBER
COMMENTARY
9012-33-3
c.f. EC 3.2.1.165
9027-52-5
deleted
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
4-methylumbelliferone + N-acetyl-beta-D-glucosamine
-
-
-
?
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide + H2O
N-acetyl-beta-D-glucosamine + 4-methylumbelliferone
-
-
-
?
4-nitrophenyl beta-N-acetyl-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-galactosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-galactosamine
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O
?
-
-
-
?
4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O
4-nitrophenol + N-acetyl-beta-D-glucosamine
-
-
-
-
?
4-nitrophenyl N-acetyl-D-glucosaminide + H2O
N-acetylglucosamine + 4-nitrophenol
-
-
-
-
?
additional information
?
-
it can not hydrolyze the complex N-glycan substrate (GlcNAcbeta-1,2Manalpha-1,6)(GlcNAcbeta-1,2Manalpha-1,3)Manbeta-1,4GlcNAcbeta-1,4GlcNAc-PA as well as the long polymer chitin
-
-
?
additional information
?
-
-
it can not hydrolyze the complex N-glycan substrate (GlcNAcbeta-1,2Manalpha-1,6)(GlcNAcbeta-1,2Manalpha-1,3)Manbeta-1,4GlcNAcbeta-1,4GlcNAc-PA as well as the long polymer chitin
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-acetamido-2-deoxy-N-([4-[(2,4-dichlorobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-beta-D-glucopyranosylamine
54.5% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-([4-[(2,6-dichlorobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-beta-D-glucopyranosylamine
23.5% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-([4-[(2-fluorobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-beta-D-glucopyranosylamine
19.9% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-([4-[(2-hydroxybenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-beta-D-glucopyranosylamine
19.3% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-([4-[(2-methoxybenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-alpha-D-glucopyranosylamine
32% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-([4-[(2-nitrobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-beta-D-glucopyranosylamine
21% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-([4-[(3-fluorobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-beta-D-glucopyranosylamine
19.8% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-([4-[(3-hydroxybenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-beta-D-glucopyranosylamine
17.6% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-([4-[(3-methoxybenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-beta-D-glucopyranosylamine
17.4% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-([4-[(3-nitrobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-beta-D-glucopyranosylamine
24.5% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-([4-[(4-fluorobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-beta-D-glucopyranosylamine
24.3% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-([4-[(4-nitrobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-beta-D-glucopyranosylamine
17.8% inhibition at 0.1 mM
2-acetamido-2-deoxy-N-[(4-[[2-(trifluoromethyl)benzamido]methyl]-1H-1,2,3-triazol-1-yl)acetyl]-beta-D-glucopyranosylamine
22.6% inhibition at 0.1 mM
2-acetamido-N-([4-[(2-amino-3-methylbenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-2-deoxy-beta-D-glucopyranosylamine
13.6% inhibition at 0.1 mM
2-acetamido-N-([4-[(2-chlorobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-2-deoxy-beta-D-glucopyranosylamine
9.8% inhibition at 0.1 mM
2-acetamido-N-([4-[(3-aminobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-2-deoxy-beta-D-glucopyranosylamine
16.6% inhibition at 0.1 mM
2-acetamido-N-([4-[(3-chlorobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-2-deoxy-beta-D-glucopyranosylamine
15.7% inhibition at 0.1 mM
2-acetamido-N-([4-[(4-chlorobenzamido)methyl]-1H-1,2,3-triazol-1-yl]acetyl)-2-deoxy-beta-D-glucopyranosylamine
23.4% inhibition at 0.1 mM
2-acetamido-N-[(4-[[([1,1'-biphenyl]-3-carbonyl)amino]methyl]-1H-1,2,3-triazol-1-yl)acetyl]-2-deoxy-beta-D-glucopyranosylamine
44.1% inhibition at 0.1 mM
2-acetamido-N-[(4-[[([1,1'-biphenyl]-4-carbonyl)amino]methyl]-1H-1,2,3-triazol-1-yl)acetyl]-2-deoxy-beta-D-glucopyranosylamine
17.6% inhibition at 0.1 mM
2-acetamido-N-[(4-[[4-chloro-2-(propan-2-yl)benzamido]methyl]-1H-1,2,3-triazol-1-yl)acetyl]-2-deoxy-beta-D-glucopyranosylamine
31.1% inhibition at 0.1 mM
2-amino-1H-benzo[de]isoquinoline-1,3(2H)-dione
28.1% inhibition at 0.1 mM
2-[(2-acetamido-2-deoxy-beta-D-glucopyranosyl)thio]-N-(81-[5-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)pentyl]-1H-1,2,3-triazol-4-yl]methyl)acetamide
82.8% inhibition at 0.1 mM
2-[(2-acetamido-2-deoxy-beta-D-glucopyranosyl)thio]-N-([1-[3-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)propyl]-1H-1,2,3-triazol-4-yl]methyl)acetamide
83.6% inhibition at 0.1 mM
2-[(2-acetamido-2-deoxy-beta-D-glucopyranosyl)thio]-N-([1-[4-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)butyl]-1H-1,2,3-triazol-4-yl]methyl)acetamide
86.2% inhibition at 0.1 mM
2-[[1-[(2-acetamido-beta-D-glucopyranosylthiomethyl)-1H-1,2,3-triazol-4-yl]methyl]-1H-benzo[de]isoquinoline-1,3(2H)-dione
17.9% inhibition at 0.1 mM
2-[[1-[2-[(2-acetamido-beta-D-glucopyranosyl)thio]ethyl]-1H-1,2,3-triazol-4-yl]methyl]-1H-benzo[de]isoquinoline-1,3(2H)-dione
84% inhibition at 0.1 mM
2-[[1-[3-[(2-acetamido-beta-D-glucopyranosyl)thio]propyl]-1H-1,2,3-triazol-4-yl]methyl]-1H-benzo[de]isoquinoline-1,3(2H)-dione
81.6% inhibition at 0.1 mM
2-[[1-[4-[(2-acetamido-beta-D-glucopyranosyl)thio]butyl]-1H-1,2,3-triazol-4-yl]methyl]-1H-benzo[de]isoquinoline-1,3(2H)-dione
89.9% inhibition at 0.1 mM
2-[[1-[5-[(2-acetamido-beta-D-glucopyranosyl)thio]pentyl]-1H-1,2,3-triazol-4-yl]methyl]-1H-benzo[de]isoquinoline-1,3(2H)-dione
91.9% inhibition at 0.1 mM
2-[[1-[6-[(2-acetamido-beta-D-glucopyranosyl)thio]hexyl]-1H-1,2,3-triazol-4-yl]methyl]-1H-benzo[de]isoquinoline-1,3(2H)-dione
93.2% inhibition at 0.1 mM
allyl ((1-(2-((2-acetamido-2-deoxy-beta-D-glucopyrano-syl)amino)-2-oxoethyl)-1H-1,2,3-triazol-4-yl)methyl)carbamate
25.7% inhibition at 0.1 mM
N-((1-(2-((2-acetamido-2-deoxy-beta-D-glucopyranosyl)amino)-2-oxoethyl)-1H-1,2,3-triazol-4-yl)methyl)benzamide
29.2% inhibition at 0.1 mM
N-((1-(2-((2-acetamido-2-deoxy-beta-D-glucopyranosyl)amino)-2-oxoethyl)-1H-1,2,3-triazol-4-yl)methyl)picolinamide
22.6% inhibition at 0.1 mM
N-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)-2-[(2-acetamido-beta-D-glucopyranosyl)thio]acetamide
24.9% inhibition at 0.1 mM
N-[2-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)ethyl]-2-[(2-acetamido-beta-D-glucopyranosyl) thio]acetamide
18.4% inhibition at 0.1 mM
N-[2-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)ethyl]-2-[(2-acetamido-beta-D-glucopyranosyl)thio]acetamide
18.4% inhibition at 0.1 mM
N-[2-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)ethyl]-3-[(2-acetamido-beta-D-glucopyranosyl)thio]propanamide
25.8% inhibition at 0.1 mM
N-[3-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)propyl]-2-[(2-acetamido-beta-D-glucopyranosyl)thio]acetamide
29.2% inhibition at 0.1 mM
N-[3-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)propyl]-3-[(2-acetamido-beta-D-glucopyranosyl)thio]propanamide
25.3% inhibition at 0.1 mM
N-[4-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)butyl]-2-[(2-acetamido-beta-D-glucopyranosyl)thio]acetamide
29.1% inhibition at 0.1 mM
N-[4-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)butyl]-3-[(2-acetamido-beta-D-glucopyranosyl)thio]propanamide
26.9% inhibition at 0.1 mM
N-[[1-[2-(1,3-dioxo-1H-benzo[de]isoquinolin-2(3H)-yl)ethyl]-1H-1,2,3-triazol-4-yl]methyl]-2-(2-acetamido-beta-D-glucopyranosylthio)acetamide
81.4% inhibition at 0.1 mM
(2Z)-2-[(2E)-[(naphthalen-1-yl)methylidene]hydrazinylidene]-3-(2-phenoxyethyl)-1,3-thiazolidin-4-one
-
56.4% inhibition at 0.1 mM
(Z)-2-(((E)-benzylidene)hydrazono)-3-(2-phenoxyethyl)thiazolidin-4-one
-
79.4% inhibition at 0.01 mM
(Z)-2-(((E)-naphthalen-1-ylmethylene)hydrazono)-3-(2-phenoxyethyl) thiazolidin-4-one
-
56.4% inhibition at 0.01 mM
(Z)-2-(((E)-naphthalen-1-ylmethylene)hydrazono)-3-(2-phenoxyethyl)thiazolidin-4-one
-
35.5% inhibition at 0.01 mM
(Z)-2-(((E)-naphthalen-1-ylmethylene)hydrazono)-3-(prop-2-yn-1-yl) thiazolidin-4-one
-
8.8% inhibition at 0.1 mM
(Z)-2-(((E)-naphthalen-1-ylmethylene)hydrazono)-3-(prop-2-yn-1-yl)thiazolidin-4-one
-
8.8% inhibition at 0.1 mM
(Z)-3-((2-chlorothiazol-5-yl)methyl)-2-(((E)-naphthalen-1-ylmethylene)hydrazono) thiazolidin-4-one
-
39% inhibition at 0.1 mM
(Z)-3-((2-chlorothiazol-5-yl)methyl)-2-(((E)-naphthalen-1-ylmethylene)hydrazono)thiazolidin-4-one
-
39% inhibition at 0.1 mM
(Z)-3-((3,5-dimethylisoxazol-4-yl)methyl)-2-(((E)-naphthalen-1-ylmethylene)hydrazono) thiazolidin-4-one
-
34.5% inhibition at 0.1 mM
(Z)-3-((3,5-dimethylisoxazol-4-yl)methyl)-2-(((E)-naphthalen-1-ylmethylene)hydrazono)thiazolidin-4-one
-
34.5% inhibition at 0.1 mM
(Z)-3-((benzyloxy)methyl)-2-(((E)-(4-bromo naphthalen-1-yl)methylene)hydrazono) thiazolidin-4-one
-
43.7% inhibition at 0.01 mM
(Z)-3-((benzyloxy)methyl)-2-(((E)-(4-bromonaphthalen-1-yl)methylene)hydrazono)thiazolidin-4-one
-
43.7% inhibition at 0.1 mM
(Z)-3-((benzyloxy)methyl)-2-(((E)-4-(dimethylamino)benzylidene)hydrazono)thiazolidin-4-one
(Z)-3-((benzyloxy)methyl)-2-(((E)-naphthalen-1-ylmethylene)hydrazono) thiazolidin-4-one
-
85.9% inhibition at 0.1 mM, 35.5% inhibition at 0.01 mM
(Z)-3-((benzyloxy)methyl)-2-(((E)-naphthalen-1-ylmethylene)hydrazono)thiazolidin-4-one
-
85.9% inhibition at 0.1 mM
(Z)-3-(4-chlorobenzyl)-2-(((E)-naphthalen-1-yl methylene)hydrazono)thiazolidin-4-one
-
53.2% inhibition at 0.1 mM
(Z)-3-(4-fluorobenzyl)-2-(((E)-naphthalen-1-yl methylene)hydrazono)thiazolidin-4-one
-
41.7% inhibition at 0.1 mM
(Z)-3-(cyclobutylmethyl)-2-(((E)-naphthalen-1-ylmethylene)hydrazono)thiazolidin-4-one
-
9.0% inhibition at 0.1 mM
(Z)-3-(cyclopropylmethyl)-2-(((E)-naphthalen-1-ylmethylene)hydrazono)thiazolidin-4-one
-
1.0% inhibition at 0.1 mM
(Z)-3-allyl-2-(((E)-naphthalen-1-ylmethylene) hydrazono)thiazolidin-4-one
-
21.9% inhibition at 0.1 mM
(Z)-3-ethyl-2-(((E)-naphthalen-1-ylmethylene) hydrazono)thiazolidin-4-one
-
28.1% inhibition at 0.1 mM
2-deoxy-2-(trimethylammonio)-beta-D-glucopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-D-glucopyranose
-
natural selective inhibitor, contains 3 GlcNAc residues
2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-D-glucopyranose
-
-
2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-D-glucopyranose
-
-
6-(dimethylamino)-2-(2-([(5-methyl-1,3,4-thiadiazol-2-yl)methyl]amino)ethyl)-1H-benzo[de]isoquinoline-1,3(2H)-dione
-
95.9% inhibition at 0.1 mM
p-methoxyphenyl 2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranoside
-
-
p-methoxyphenyl 2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranoside
-
-
p-methoxyphenyl 2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranoside
-
-
p-methoxyphenyl 2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranoside
-
-
PUGNAc
-
i.e. O-(2-acetamido-2-deoxy-D-glucopyranosylidenamino) N-phenylcarbamate
additional information
-
not inhibited by (Z)-3-methyl-2-(((E)-naphthalen-1-ylmethylene) hydrazono)thiazolidin-4-one
-
PUGNAc
i.e. O-(2-acetamido-2-deoxy-D-glucopyranosylidenamino) N-phenylcarbamate, 97.5% inhibition at 0.1 mM
TMG-chitotriomycin
mechanism of selective inhibition is related to the existence of the +1 subsite at the active pocket of OfHex1 and a key residue, Trp490, at this site. Mutation of Trp490 to Ala leads to a 2277fold decrease in sensitivity toward TMG-chitotriomycin as well as an 18-fold decrease in binding affinity for the substrate (GlcNAc)2
(Z)-2-(((E)-benzylidene)hydrazono)-3-((benzyloxy)methyl)thiazolidin-4-one
-
62.7% inhibition at 0.01 mM
(Z)-2-(((E)-benzylidene)hydrazono)-3-((benzyloxy)methyl)thiazolidin-4-one
-
62.7% inhibition at 0.1 mM
(Z)-3-((benzyloxy)methyl)-2-(((E)-4-(dimethylamino)benzylidene)hydrazono)thiazolidin-4-one
-
63.5% inhibition at 0.01 mM
(Z)-3-((benzyloxy)methyl)-2-(((E)-4-(dimethylamino)benzylidene)hydrazono)thiazolidin-4-one
-
63.5% inhibition at 0.1 mM
(Z)-3-((benzyloxy)methyl)-2-(((E)-naphthalen-2-ylmethylene)hydrazono)thiazolidin-4-one
-
59.8% inhibition at 0.1 mM
(Z)-3-((benzyloxy)methyl)-2-(((E)-naphthalen-2-ylmethylene)hydrazono)thiazolidin-4-one
-
59.8% inhibition at 0.01 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
structural elements of substrates, the 2-acetamido group and the beta-glycoside bond linkage, are essential for its activity. Transcriptional level increases dramatically before the molting stage
-
additional information
-
structural elements of substrates, the 2-acetamido group and the beta-glycoside bond linkage, are essential for its activity. Transcriptional level increases dramatically before the molting stage
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.07
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant V327G, pH 7.0, 25°C
0.095
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant H433A, pH 7.0, 25°C
0.096
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant E328A, pH 7.0, 25°C
0.1
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant W490A, pH 7.0, 25°C
0.105
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant E328Q, pH 7.0, 25°C
0.107
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
wild-type, pH 7.0, 25°C
0.149
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant W448F, pH 7.0, 25°C
0.223
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant W448A, pH 7.0, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.31
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant H433A, pH 7.0, 25°C
0.47
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant W448A, pH 7.0, 25°C
0.57
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant W448F, pH 7.0, 25°C
168
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant W490A, pH 7.0, 25°C
189
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant V327G, pH 7.0, 25°C
349
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant E328Q, pH 7.0, 25°C
356
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant E328A, pH 7.0, 25°C
434.7
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
wild-type, pH 7.0, 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2.1
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant W448A, pH 7.0, 25°C
3.3
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant H433A, pH 7.0, 25°C
3.8
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant W448F, pH 7.0, 25°C
1676
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant W490A, pH 7.0, 25°C
2700
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant V327G, pH 7.0, 25°C
3324
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant E328Q, pH 7.0, 25°C
3708
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
mutant E328A, pH 7.0, 25°C
4063
4-methylumbelliferyl N-acetyl-beta-D-glucosaminide
wild-type, pH 7.0, 25°C
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0037
(Z)-2-(((E)-benzylidene)hydrazono)-3-((benzyloxy)methyl)thiazolidin-4-one
-
at pH 6.5 and 30°C
0.0021
(Z)-2-(((E)-benzylidene)hydrazono)-3-(2-phenoxyethyl)thiazolidin-4-one
-
at pH 6.5 and 30°C
0.0071
(Z)-2-(((E)-naphthalen-1-ylmethylene)hydrazono)-3-(2-phenoxyethyl) thiazolidin-4-one
-
at pH 6.5 and 30°C
0.0082
(Z)-3-((benzyloxy)methyl)-2-(((E)-(4-bromo naphthalen-1-yl)methylene)hydrazono) thiazolidin-4-one
-
at pH 6.5 and 30°C
0.0041
(Z)-3-((benzyloxy)methyl)-2-(((E)-4-(dimethylamino)benzylidene)hydrazono)thiazolidin-4-one
-
at pH 6.5 and 30°C
0.0102
(Z)-3-((benzyloxy)methyl)-2-(((E)-naphthalen-1-ylmethylene)hydrazono) thiazolidin-4-one
-
at pH 6.5 and 30°C
0.0064
(Z)-3-((benzyloxy)methyl)-2-(((E)-naphthalen-2-ylmethylene)hydrazono)thiazolidin-4-one
-
at pH 6.5 and 30°C
0.000065
2-deoxy-2-(trimethylammonio)-beta-D-glucopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-beta-D-glucopyranosyl-(1->4)-2-(acetylamino)-2-deoxy-D-glucopyranose
-
pH 4.0, 25°C
0.000058
2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-D-glucopyranose
-
pH 4.0, 25°C
0.000077
2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-D-glucopyranose
-
pH 4.0, 25°C
0.00086
p-methoxyphenyl 2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranoside
-
pH 4.0, 25°C
0.000073
p-methoxyphenyl 2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranoside
-
pH 4.0, 25°C
0.00007
p-methoxyphenyl 2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranoside
-
pH 4.0, 25°C
0.000066
p-methoxyphenyl 2-trimethylammonium-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranosyl-(1,4)-2-acetamino-2-deoxy-beta-D-glucopyranoside
-
pH 4.0, 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
up-regulation of OfHEX1 transcription, specific to the integument during the pupation
additional information
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
knock-down by RNAi. Before pupation, most of the larvae injected with RNA1 survive without any visible changes in phenotype and appear similar to those injected with control. At the pupation stage, 20% of the RNAi-injected larvae show abnormal phenotypes that are absent in control larvae and die 1 or 2 days after pupation. All of the larvae with abnormal phenotypes fail to shed their old cuticles completely before new ones started to form underneath
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). HEXC_OSTFU
594
1
67964
Swiss-Prot
Secretory Pathway (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
homodimer
2 * 67000, MS and SDS-PAGE, 2 * 65500, sequence analysis
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme andd in complex with TMG-chitotriomycin, both to 2.1 A resolution. Enzyme displays large conformational changes linked to an open-close mechanism at the entrance of the active site, which is characterized by the lid residue, Trp448. Structure shows a homodimeric enzyme with the two monomers in the adjacent asymmetry units. Each monomer is N-glycosylated at Asn164 and Asn375. All of the 12 cysteine residues of each monomer contribute to the intradisulfide bonds
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
W448A
mutation results in a more than 1000fold loss in enzyme activity, due mainly to the effect on kcat
W490A
mutation leads to a 2277fold decrease in sensitivity toward TMG-chitotriomycin as well as an 18fold decrease in binding affinity for the substrate (GlcNAc)2
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1468fold to homogeneity with an activity yield of 20% by four column chromatography steps (anion exchange chromatography and gel filtration)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
Hex1 may be mostly involved in insect chitin catabolism and may be a specific target for pesticide development
additional information
-
Hex1 may be mostly involved in insect chitin catabolism and may be a specific target for pesticide development
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yang, Q.; Liu, T.; Liu, F.; Qu, M.; Qian, X.
A novel beta-N-acetyl-D-hexosaminidase from the insect Ostrinia furnacalis (Guenee)
FEBS J.
275
5690-5702
2008
Ostrinia furnacalis (Q06GJ0), Ostrinia furnacalis
Yang, Y.; Liu, T.; Yang, Y.; Wu, Q.; Yang, Q.; Yu, B.
Synthesis, evaluation, and mechanism of N,N,N-trimethyl-D-glucosamine-(1-4)-chitooligosaccharides as selective inhibitors of glycosyl hydrolase family 20 beta-N-acetyl-D-hexosaminidases
ChemBioChem
12
457-467
2011
Ostrinia furnacalis, Streptomyces plicatus (O85361)
Liu, T.; Zhang, H.; Liu, F.; Wu, Q.; Shen, X.; Yang, Q.
Structural determinants of an insect beta-N-Acetyl-D-hexosaminidase specialized as a chitinolytic enzyme
J. Biol. Chem.
286
4049-4058
2011
Ostrinia furnacalis (Q06GJ0), Ostrinia furnacalis
Chen, W.; Shen, S.; Dong, L.; Zhang, J.; Yang, Q.
Selective inhibition of beta-N-acetylhexosaminidases by thioglycosyl-naphthalimide hybrid molecules
Bioorg. Med. Chem.
26
394-400
2018
Ostrinia furnacalis (Q06GJ0)
Yang, H.; Liu, T.; Qi, H.; Huang, Z.; Hao, Z.; Ying, J.; Yang, Q.; Qian, X.
Design and synthesis of thiazolylhydrazone derivatives as inhibitors of chitinolytic N-acetyl-beta-D-hexosaminidase
Bioorg. Med. Chem.
26
5420-5426
2018
Ostrinia furnacalis
Dong, L.; Shen, S.; Chen, W.; Lu, H.; Xu, D.; Jin, S.; Yang, Q.; Zhang, J.
Glycosyl triazoles as novel insect beta-N-acetylhexosaminidase OfHex1 inhibitors Design, synthesis, molecular docking and MD simulations
Bioorg. Med. Chem.
27
2315-2322
2019
Homo sapiens, Ostrinia furnacalis (Q06GJ0)
EXTERNAL LINKS (specific for EC-Number 3.2.1.52)
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
