Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 3.2.1.52 - beta-N-acetylhexosaminidase and Organism(s) Vibrio furnissii and UniProt Accession P96155

for references in articles please use BRENDA:EC3.2.1.52
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
Acts on N-acetylglucosides and N-acetylgalactosides.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Vibrio furnissii
UNIPROT: P96155
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
hide
The taxonomic range for the selected organisms is: Vibrio furnissii
The enzyme appears in selected viruses and cellular organisms
Synonyms
beta-hexosaminidase, hexosaminidase, n-acetyl-beta-glucosaminidase, o-glcnacase, hex a, n-acetylglucosaminidase, hexosaminidase a, beta-n-acetylhexosaminidase, hex b, beta-hexosaminidase a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2-acetamido-2-deoxy-beta-D-glucoside acetamidodeoxyglucohydrolase
-
-
-
-
65 kDa epididymal boar protein
-
-
-
-
beta-acetylaminodeoxyhexosidase
-
-
-
-
beta-acetylhexosaminidase
-
-
-
-
beta-acetylhexosaminidinase
-
-
-
-
beta-D-hexosaminidase
-
-
-
-
beta-D-N-acetylhexosaminidase
-
-
-
-
Beta-GlcNAcase
-
-
-
-
beta-hexosaminidase
-
-
-
-
beta-N-acetyl-D-hexosaminidase
-
-
-
-
beta-N-acetyl-hexosaminidase
-
-
-
-
beta-N-acetylgalactosaminidase
-
-
-
-
beta-N-acetylglucosaminidase
-
-
-
-
Beta-N-acetylhexosaminidase
-
-
-
-
beta-NAHA
-
-
-
-
Beta-NAHASE
-
-
-
-
chitobiase
-
-
-
-
Hex
-
-
-
-
hexosaminidase
-
-
-
-
hexosaminidase A
-
-
-
-
N-acetyl-beta-D-hexosaminidase
-
-
-
-
N-acetyl-beta-glucosaminidase
-
-
-
-
N-acetyl-beta-hexosaminidase
-
-
-
-
N-acetylhexosaminidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
O-glycosyl bond hydrolysis
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-N-acetyl-D-hexosaminide N-acetylhexosaminohydrolase
Acts on N-acetylglucosides and N-acetylgalactosides.
CAS REGISTRY NUMBER
COMMENTARY hide
9012-33-3
c.f. EC 3.2.1.165
9027-52-5
deleted
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl-2-acetamido-2-deoxy-beta-D-glucopyranoside + H2O
4-methylumbelliferone + 2-acetamido-2-deoxy-beta-D-glucopyranoside
show the reaction diagram
-
-
-
?
aryl-beta-N-acetylgalactosaminide + H2O
a phenol + beta-N-acetylgalactosamine
show the reaction diagram
-
-
-
?
aryl-beta-N-acetylglucosaminide + H2O
? + beta-N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
chitin oligosaccharides + H2O
N-acetyl-D-glucosamine
show the reaction diagram
(N-acetyl-D-glucosamine)2-6
-
-
?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
?
show the reaction diagram
-
-
-
?
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
?
show the reaction diagram
-
-
-
?
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N-diacetylchitobiose
show the reaction diagram
-
-
-
?
N,N',N''-triacetylchitotriose + H2O
N-acetyl-D-glucosamine + N,N'-diacetylchitobiose
show the reaction diagram
-
-
-
?
N,N'-diacetylchitobiose + H2O
deacetylated chitooligosaccharides + acetate
show the reaction diagram
-
-
-
?
N-acetyl-beta-D-hexosaminides + H2O
N-acetyl-D-hexosamines
show the reaction diagram
-
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside + H2O
2-acetamido-2-deoxy-D-galactose + p-nitrophenol
show the reaction diagram
-
-
-
?
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside + H2O
2-acetamido-2-deoxy-D-glucose + p-nitrophenol
show the reaction diagram
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
N-acetyl-beta-D-hexosaminides + H2O
N-acetyl-D-hexosamines
show the reaction diagram
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(N-acetyl-D-glucosamine)2
non-competitive
(N-acetyl-D-glucosamine)3-6
competitive inhibition
Hg2+
with 1 mM 50% of activity remains, with 5 mM 10% of activity remains
KCl
with 1 mM 80% of activity remains, with 2 mM 50% of activity remains
NaCl
with 1 mM 80% of activity remains, with 2 mM 50% of activity remains
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.7
(N-acetyl-D-glucosamine)2
-
0.83
(N-acetyl-D-glucosamine)3
-
0.78
(N-acetyl-D-glucosamine)4
-
3.7
(N-acetyl-D-glucosamine)5
-
3.5
(N-acetyl-D-glucosamine)6
-
0.33
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-galactopyranoside
-
0.09
p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
200
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.8
substrate: (N-acetyl-D-glucosamine)2
7
substrate: (N-acetyl-D-glucosamine)3-6
7 - 8
substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.5 - 9.5
full activity at pH 7.5 after preincubation for 15 min in that range, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
6
50% of activity, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 40
at pH 7.5, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HEX1_VIBFU
611
0
69502
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
69380
amino acid calculation
70000
SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
no activity, substrate: (N-acetyl-D-glucosamine)2
393685
8 - 8.3
50-60% of activity with (N-acetyl-D-glucosamine)3-6 as substrate, no activity with (N-acetyl-D-glucosamine)2 as substrate
393685
9
50% of activity, substrate: p-nitrophenyl-beta-2-acetamido-2-deoxy-D-glucopyranoside
393685
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
stable for at least 24 h at room temperature
37
stable for at least 24 h
4 - 42
stable for 15 min, at pH 7.5
65
complete loss of activity after 15 min at pH 7.5
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-70°C, purified enzyme, stable for at least 6 months
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
40fold to homogeneity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
into Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Keyhani, N.O.; Roseman, S.
The chitin catabolic cascade in the marine bacterium Vibrio furnissii: Molecular cloning, isolation, and characterization of a periplasmic beta-N-acetylhexosaminidase
J. Biol. Chem.
271
33425-33432
1996
Vibrio furnissii (P96155), Vibrio furnissii
Manually annotated by BRENDA team