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Information on EC 3.2.1.51 - alpha-L-fucosidase and Organism(s) Homo sapiens and UniProt Accession P04066

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UNIPROT: P04066 not found.
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The taxonomic range for the selected organisms is: Homo sapiens
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
alpha-l-fucosidase, acid hydrolase, fuca1, serum alpha-l-fucosidase, serum afu, alpha-fuc, fuca2, alpha-l-fucosidase i, alphafuc, alf1_wf, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-L-fucosidase
-
alpha-L-fucosidase 1
-
alpha-L-fucosidase I
-
Alpha-L-fucoside fucohydrolase
-
acid hydrolase
-
-
alpha-fucosidase
-
-
-
-
alpha-L fucosidase
-
-
alpha-L-fucosidase
Alpha-L-fucoside fucohydrolase
FucA
-
-
Fuca2
-
-
serum AFU
-
-
serum alpha-L-fucosidase
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
-
hydrolysis
-
of glycosidic bond
hydrolysis of O-glycosyl bond
-
-
-
-
transglycosylation
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-L-fucoside fucohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
9037-65-4
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-chloro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-chloro-4-nitrophenol + L-fucose
show the reaction diagram
pH 6.8, 37°C
-
-
?
2-fluoro-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-fluoro-4-nitrophenol + L-fucose
show the reaction diagram
pH 6.8, 37°C
-
-
?
2-methyl-4-nitrophenyl alpha-L-fucopyranoside + H2O
2-methyl-4-nitrophenol + L-fucose
show the reaction diagram
pH 6.8, 37°C
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
show the reaction diagram
-
-
-
?
4-nitrophenyl alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
show the reaction diagram
sodium acetate buffer 50 mM, pH 5.0, 37°C
-
-
?
m-nitrophenyl alpha-L-fucopyranoside + H2O
m-nitrophenol + L-fucose
show the reaction diagram
pH 6.8, 37°C
-
-
?
p-cyanophenyl alpha-L-fucopyranoside + H2O
p-cyanophenol + L-fucose
show the reaction diagram
pH 6.8, 37°C
-
-
?
p-nitrophenyl-alpha-L-fucopyranoside + H2O
p-nitrophenol + alpha-L-fucose
show the reaction diagram
-
-
-
?
phenyl alpha-L-fucopyranoside + H2O
phenol + L-fucose
show the reaction diagram
pH 6.8, 37°C
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside
4-methylumbelliferone + alpha-L-fucose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-L-fucopyranoside + H2O
4-methylumbelliferone + alpha-L-fucose
show the reaction diagram
4-nitrophenyl-alpha-L-fucopyranoside
?
show the reaction diagram
-
assay at pH 4.5, 37°C for 3 h, reaction stopped by addition of borate buffer
-
-
?
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + alpha-L-fucose
show the reaction diagram
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
show the reaction diagram
Fuc-alpha-(1-2)-Fuc-beta-4-nitrophenol + H2O
alpha-L-fucose + Fuc-beta-4-nitrophenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GalNAc-alpha-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-N-acetyl-galactosaminyl-phenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol + H2O
alpha-L-fucose + beta-galactosyl-4-nitrophenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Glc + H2O
alpha-L-fucose + D-glucose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Xyl + H2O
alpha-L-fucose + D-xylose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-[Gal-alpha-(1-3)]-Gal-beta-phenol + H2O
alpha-L-fucose + Gal-alpha-(1-3)-Gal-beta-phenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-Glc + H2O
alpha-L-fucose + D-glucose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-beta-O(CH2)6NHCOCF3 + H2O
alpha-L-fucose + GlcNAc-beta-O(CH2)6NHCOCF3
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc-phenol + H2O
alpha-L-fucose + N-acetyl-glucosaminyl-beta-phenol
show the reaction diagram
-
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc-beta-phenol + H2O
alpha-L-fucose + beta-N-acetyl-glucosaminyl-phenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-4)-[Gal-beta-(1-3)]-GlcNAc-beta-phenol + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-phenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
Fuc-alpha-(1-6)-GlcNAc-4-nitrophenol + H2O
alpha-L-fucose + GlcNAc-4-nitrophenol
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-[Gal-beta-(1-3)]-GlcNAc-beta-OCH3 + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-OCH3
show the reaction diagram
-
-
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
show the reaction diagram
-
low activity
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
p-nitrophenyl alpha-L-fucoside + H2O
p-nitrophenol + alpha-L-fucose
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
show the reaction diagram
-
-
-
-
?
Fuc-alpha-(1-2)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc + H2O
alpha-L-fucose + lactose
show the reaction diagram
Fuc-alpha-(1-2)-Glc + H2O
alpha-L-fucose + D-glucose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-2)-Xyl + H2O
alpha-L-fucose + D-xylose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-Glc + H2O
alpha-L-fucose + D-glucose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-3)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-4)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-Gal + H2O
alpha-L-fucose + D-galactose
show the reaction diagram
-
-
-
?
Fuc-alpha-(1-6)-GlcNAc + H2O
alpha-L-fucose + N-acetyl-D-glucosamine
show the reaction diagram
Fuc-alpha-(1-6)-GlcNAc-beta-Asn + H2O
alpha-L-fucose + N-acetyl-D-glucosaminyl-Asn
show the reaction diagram
-
-
-
?
GalNAc-alpha-(1-3)-Gal-[Fuc-alpha-(1-2)] + H2O
alpha-L-fucose + GalNAc-alpha-(1-3)-Gal
show the reaction diagram
-
low activity
-
?
lacto-N-fucopentaose II + H2O
alpha-L-fucose + Gal-beta-(1-3)-GlcNAc-beta-(1-3)-Gal-beta-(1-4)-Glc
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
12% activation at 2 mM
Mn2+
-
22% stimulation at 5 mM
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
(2R,3R,4R,5R,6S)-2-(aminomethyl)-6-methylpiperidine-3,4,5-triol
-
(2S,3R,4R,5R)-5-(3-aminopropoxy)-2-methylpiperidine-3,4-diol
-
(2S,3R,4R,5R,6S)-2-(hydroxymethyl)-6-methylpiperidine-3,4,5-triol
-
(2S,3R,4S,5R)-1-(3-aminopropyl)-2-methylpiperidine-3,4,5-triol
-
(2S,3R,4S,5R)-2-benzylpiperidine-3,4,5-triol
-
(2S,3R,4S,5R)-2-isopropylpiperidine-3,4,5-triol
-
2-cyclopentyl-2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)acetamide
-
2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)quinoline-4-carboxamide
-
3-(1H-indol-3-yl)-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)propanamide
-
5-fluoro-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)-1H-indole-2-carboxamide
-
deoxyfuconojirimycin
-
fuconojirimycin
majority of C1-substituted fuconojirimycins act as reversible inhibitors of the human fucosidase
(1R,2R,3R,4R,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
-
-
(1R,2R,3R,4S,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
-
-
(R)-2-aminobutanol
-
-
(S)-2-aminobutanol
-
-
1,5-dideoxy-1,5-([(2R,3R)-2,4-dihydroxy-3-(sulfooxy)butyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-([(2R,3S,4R,5R)-2,4,5,6-tetrahydroxy-3-(sulfooxy)hexyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-([(2S,3S)-2,4-dihydroxy-3-(sulfooxy)butyl]-episulfoniumylidene)-L-fucitol inner salt
-
-
1,5-dideoxy-1,5-[5-methoxycarbonyl-1-pentyl-episulfoniumylidene]-L-fucitol tetrafluoroborate salt
-
-
1,5-dideoxy-1,5-[methyl-episulfoniumylidene]-L-fucitol tetrafluoroborate salt
-
-
4-chloromercuribenzoate
-
92% inhibition at 0.1 mM
alpha-L-fuco-N-benzyl aminocyclopentitol
-
-
Cu2+
-
88% inhibition at 2 mM
D-glucose
-
21% inhibition at 6 mM
deoxycholate
-
36% inhibition at 0.012%, w/v
deoxyfuconojirimycin
-
inhibits by 94-100% at 1 mM
Fe3+
-
40% inhibition at 2 mM
formaldehyde
-
57% inhibition at 160 mM
L-cysteine
-
20% inhibition at 30 mM
Mn2+
-
26% inhibition at 2 mM
Sn2+
-
24% inhibition at 2 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Sodium azide
1M, enhanced enzyme activity, only in mutants
bovine serum albumin
-
increases acitivity up to 100%
-
chemokine CCL1
-
1.8-fold increase of ALP-expression after 24-h stimulation
-
chemokine CCL5
-
2.2-fold increase of ALP-expression after 24-h stimulation
-
dithiothreitol
-
13% activation at 0.2 mM
KCl
-
17% activation at 300 mM
additional information
-
cell-bound enzyme activity increases sharply following permeabilization of intact sperm
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.063
2-chloro-4-nitrophenyl alpha-L-fucopyranoside
mutant E289G
0.11 - 1.06
p-nitrophenyl alpha-L-fucopyranoside
0.06 - 0.08
4-methylumbelliferyl alpha-L-fucopyranoside
0.05 - 0.3
4-methylumbelliferyl-alpha-L-fucopyranoside
0.168 - 0.43
4-nitrophenyl-alpha-L-fucopyranoside
2.9
Fuc-alpha-(1-2)-Gal
-
-
1.56
Fuc-alpha-(1-2)-Gal-beta-(1-4)-Glc
-
-
0.52
Fuc-alpha-(1-2)-Gal-beta-4-nitrophenol
-
-
1.63
Fuc-alpha-(1-3)-GlcNAc-phenol
-
-
2.01
Fuc-alpha-(1-4)-GlcNAc-beta-phenol
-
-
0.25
p-nitrophenyl alpha-L-fucoside
-
pH 6.8, 25°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.003 - 17.1
p-nitrophenyl alpha-L-fucopyranoside
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0000152
(2R,3R,4R,5R,6S)-2-(aminomethyl)-6-methylpiperidine-3,4,5-triol
-
0.000018
2-cyclopentyl-2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)acetamide
-
0.0000117
2-phenyl-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)quinoline-4-carboxamide
-
0.0000056
3-(1H-indol-3-yl)-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)propanamide
-
0.0000097
5-fluoro-N-(((2R,3R,4R,5R,6S)-3,4,5-trihydroxy-6-methylpiperidin-2-yl)methyl)-1H-indole-2-carboxamide
-
14
(1R,2R,3R,4R,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
-
pH 6.8, 25°C
22
(1R,2R,3R,4S,5R)-4-amino-5-methylcyclopentane-1,2,3-triol
-
pH 6.8, 25°C
200
(R)-2-aminobutanol
-
pH 6.8, 25°C
100
(S)-2-aminobutanol
-
pH 6.8, 25°C
0.3 - 18
alpha-L-fuco-N-benzyl aminocyclopentitol
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.000064
(2R,3R,4R,5R,6S)-2-(aminomethyl)-6-methylpiperidine-3,4,5-triol
Homo sapiens
-
0.000267
(2S,3R,4R,5R)-5-(3-aminopropoxy)-2-methylpiperidine-3,4-diol
Homo sapiens
-
0.000052
(2S,3R,4R,5R,6S)-2-(hydroxymethyl)-6-methylpiperidine-3,4,5-triol
Homo sapiens
-
0.00007
(2S,3R,4S,5R)-1-(3-aminopropyl)-2-methylpiperidine-3,4,5-triol
Homo sapiens
-
0.007
(2S,3R,4S,5R)-2-benzylpiperidine-3,4,5-triol
Homo sapiens
-
0.006
(2S,3R,4S,5R)-2-isopropylpiperidine-3,4,5-triol
Homo sapiens
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0012
-
HT-29 cells, plasma membrane
0.003
-
non-differentiated HT-29 cells
0.0043
-
HT-29 cells, crude extract
0.0057
-
colonic mucosa, crude extract
0.0068
-
colonic mucosa, plasma membrane
0.009
-
differentiated HT-29 cells
1.8
-
alpha-L-fucosidase B
1.93
-
-
10.66
-
-
24.2
-
-
3.81
-
alpha-L-fucosidase A
39.7
-
-
4.76
-
-
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.9 - 4
-
major pH-optimum
4 - 4.5
-
a broad acidic optimum at pH 4.0-4.5 and a second neutral optimum at pH 6.4-7.4
4.3
-
one of two pH-optima
4.5
-
assay at
4.6
-
one of two pH-optima
5.3
-
alpha-fucosidase I
5.4
-
deglycosylated enzyme
5.4 - 5.6
-
-
6
-
for 4-methylumbelliferyl-alpha-L-fucopyranoside
6 - 6.5
-
-
6.3
-
second pH-optimum for native and deglycosylated enzymes
6.4 - 7.4
-
a broad acidic optimum at pH 4.0-4.5 and a second neutral optimum at pH 6.4-7.4
6.5
-
second pH-optimum
6.8
-
one of two pH-optima
6.8 - 7.3
-
-
6.9
-
second minor pH-optimum
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.8 - 5.2
recombinant enzyme
6 - 6.5
enzyme in active form
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
-
assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6
-
isoelectric focusing, possible minor isoform
6.5
-
isoelectric focusing, isoform I
7.1
-
isoelectric focusing, isoform II
additional information
-
isoelectric focusing indicates that the purified enzyme consists of multiple isoforms
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
gastric cancer cell. Cells infected with Helicobacter pylori secrete isoform Fuc2A
Manually annotated by BRENDA team
-
not detected in adjacent tissue
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
of liver cancer cell, not detected in adjacent tissue
-
Manually annotated by BRENDA team
-
10-20% of the total cellular enzyme
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
FUCO_HUMAN
466
0
53689
Swiss-Prot
Secretory Pathway (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
-
gel filtration, alpha-fucosidase I
109000 - 116000
-
gel filtration, soluble form
200000
-
gel filtration
230000
236000
-
gel filtration
270000
-
gel filtration at pH 7.0
305000
-
gel filtration at pH 5.0 or 6.0, molecular weight depends on pH-value
390000
-
gel filtration at pH 5.0
50000
51000
-
x * 51000, SDS-PAGE
55000
-
x * 55000, SDS-PAGE
86000
-
major form
93800
-
membrane-bound form
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
-
alpha-fucosidase I, 2 * 50000, SDS-PAGE, can aggregate to tetra- and hexamers
monomer
-
alpha-fucosidase II, 1 * 50000, SDS-PAGE, less-biologically active form, can aggregate to tetra- and hexamers
oligomer
tetramer
additional information
-
comparison of elution profile of enzyme from undifferentiated and differentiated HT-29 cells through gel filtration and SDS-PAGE, presence of a 50000 Da protein in both cultures
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
sialoglycoprotein
-
the enzyme occurs in different isoforms depending on the number of terminal sialic acid residues present on the glycoprotein molecules
additional information
-
treatment of enzyme with neuraminidase does not change its isoform profile
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 8
4 h, above and below decrease in enzyme activity
696304
7
55°C, 4 h
696304
7
-
5% CO2 and high humidity or ambient air, 37°C, 24 h, 20-100% loss of activity, depending on conditions
698301
additional information
-
comparison of pH stability of enzyme from undifferentiated and differentiated HT-29 cells, enzymes from undifferentiated and differentiated HT-29 cells are very stable at neutral and basic pH values
655733
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
pH 7.0, 4 h
100
-
denatured by heating over 100°C for 5 min
4 - 55
-
highly stable
50 - 55
-
greatly decreased thermostability in fucosidosis patients
51
-
rapid inactivation for alpha-L-fucosidase A, form B more heat-stable, stabilizing effect of bovine serum albumin, addition of 0.5 M potassium thiocyanate leads to more heat sensitivity for both forms
65
-
completely abolished
70
-
60% loss of activity
additional information
-
comparison of heat stability of enzyme from undifferentiated and differentiated HT-29 cells
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
In the presence of 0.015% Triton X-100 the frozen enzyme retains 100% activity after 3 months, in absence of 0.015% Triton X-100 the enzyme loses all activity after 2 weeks. In the presence or absence of 0.015% Triton X-100 and 2-4°C retains 100% activity after 2 weeks.
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
55°C, LB medium, 4 h
-10°C, 3-6% loss of activity after 3 days
-
-20°C, fully active after 29 days, 0-4°C, 10 mM Na2HPO4, pH 5.5, 0.02% NaN3, w/v, 100 mM L-fucose, 163 days
-
-20°C, in the presence of 1% fucose and presence or absence of 0.015% Triton X-100, 100% activity after 2 weeks
-
-20°C, pH 5.5 in presence of 1% L-fucose, 100% activity after 6 weeks
-
2-4°C, in the presence of 1% fucose and 0.015% Triton X-100, 100% activity after 3 months
-
2-4°C, pH 5.5 in presence of 1% L-fucose, 77% activity after 6 weeks
-
21°C, pH 5.5 in presence of 1% L-fucose, 100% activity after 3 days
-
37°C, 5% CO2, ambient air, pH 7.0, 24 h loss of activity and increase of the pH-value, membrane associated enzyme
-
37°C, 5% CO2, high humidity, 24 h, 70% loss of activity, enzyme in semen from storage in liquid nitrogen
-
37°C, 5% CO2, high humidity, 24 h, 90% loss of activity, enzyme in seminal plasma
-
37°C, 5% CO2, high humidity, 72 h, no activity, enzyme in seminal plasma
-
37°C, 5% CO2, high humidity, pH 7.0, 24 h, 20% loss of activity, membrane associated enzyme
-
37°C, 5% CO2, high humidity, pH 7.0, 72 h, 30% loss of activity, membrane associated enzyme
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by ion-exchange chromatography, more than 95% homogeneity
near homogeneity, 1step affinity chromatography
-
to homogeneity, 2step affinity chromatography
-
to homogeneity, affinity chromatography
-
to homogeneity, affinity chromatography, isoelectric focusing, 2 forms, alpha-L-fucosidase A and B
-
to homogeneity, two forms: alpha-fucosidase I and II, affinity chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli stream BL21(DE3)
through pET21b expression vector
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
there is a greater than 139fold increase in enzyme mRNA expression in breast tumor samples compared with normal breast tissue
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
alpha-L-fucosidase is a valuable diagnostic biomarker for hepatocellular carcinoma. An ultrasensitive plasmonic biosensor converts plasmonic absorption to electrical current in order to detect alpha-L-fucosidase and cardiac troponin I using whole human blood in a real-time and parallel fashion. The detection limit is calculated to be 0.016 U/L for alpha-L-fucosidase and 0.015 ng/mL for cardiac troponin I, respectively. The developed biosensor exhibits a promising application for the prediction of cancers and many other diseases
medicine
the enzyme is an indicator of poor prognosis for patients with advanced-stage triple-negative breast cancer
diagnostics
medicine
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Piesecki, S.; Alhadeff, J.A.
The effect of carbohydrate removal on the properties of human liver alpha-L-fucosidase
Biochim. Biophys. Acta
1119
194-200
1992
Homo sapiens
Manually annotated by BRENDA team
Chuang, N.N.; Yeh, C.C.; Lin, K.S.
The basic isoelectric form of alpha-L-fucosidase from the hepatopancreas of the shrimp Penaeus monodon (crustacea: decapoda)
Comp. Biochem. Physiol. B
99
395-398
1991
Homo sapiens, Penaeus monodon
Manually annotated by BRENDA team
Laury-Kleintop, L.D.; Damjanov, I.; Alhadeff, J.A.
Characterization of mouse liver alpha-L-fucosidase. Demonstration of unusual basic isoelectric forms of the enzyme that appear to be developmentally regulated
Biochem. J.
230
75-82
1985
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Hopfer, R.L.; Alhadeff, J.A.
Solubilization and characterization of pellet-associated human brain alpha-L-fucosidase activity
Biochem. J.
229
679-685
1985
Homo sapiens
Manually annotated by BRENDA team
DiCioccio, R.A.; Barlow, J.J.; Matta, K.L.
Substrate specificity and other properties of alpha-L-fucosidase from human serum
J. Biol. Chem.
257
714-718
1982
Homo sapiens
Manually annotated by BRENDA team
Chien, S.F.; Dawson, G.
Purification and properties of two forms of human alpha-L-fucosidase
Biochim. Biophys. Acta
614
476-488
1980
Homo sapiens
Manually annotated by BRENDA team
Ahadeff, J.A; Andrews-Smith, G.L.
Purification and characterization of alpha-L-fucosidase from the liver of a fucosidosis patient
Biochem. J.
187
45-51
1980
Homo sapiens
Manually annotated by BRENDA team
Kress, B.C.; Freeze, H.H.; Herd, J.K.; Alhadeff, J.A.; Miller, A.L.
Purification and characterization of I-cell disease alpha-L-fucosidase
J. Biol. Chem.
255
955-961
1980
Homo sapiens
Manually annotated by BRENDA team
Turner, B.M.
Purification and characterization of alpha-L-fucosidase from human placenta. pH-dependent changes in molecular size
Biochim. Biophys. Acta
578
325-336
1979
Homo sapiens
Manually annotated by BRENDA team
Di Matteo, G.; Orfeo, M.A.; Romeo, G.
Human alpha-fucosidase. Purification and properties
Biochim. Biophys. Acta
429
527-537
1976
Homo sapiens
Manually annotated by BRENDA team
Alhadeff, J.A.; Miller, A.L.; Wenaas, H.; Vedvick, T.; O'Brien, J.S.
Human liver alpha-L-fucosidase. Purification, characterization, and immunochemical studies
J. Biol. Chem.
250
7106-7113
1975
Homo sapiens
Manually annotated by BRENDA team
Avila, J.L.; Convit, J.
Studies on human polymorphonuclear leukocyte enzymes. IV. Intracellular distribution and properties of alpha-L-fucosidase
Biochim. Biophys. Acta
358
308-318
1974
Homo sapiens
-
Manually annotated by BRENDA team
Alhadeff, J.A.; Miller, A.L.; O'Brien, J.S.
Purification of human placental alpha-L-fucosidase by affinity chromatography
Anal. Biochem.
60
424-430
1974
Homo sapiens
Manually annotated by BRENDA team
Khunsook, S.; Bean, B.S.; McGowan, S.R.; Alhadeff, J.A.
Purification and characterization of plasma membrane-associated human sperm alpha-L-fucosidase
Biol. Reprod.
68
709-716
2003
Homo sapiens
Manually annotated by BRENDA team
Cordero, O.J.; Merino, A.; De la Cadena, M.P.; Bugia, B.; Nogueira, M.; Vinuela, J.E.; Martinez-Zorzano, V.S.; De Carlos, A.; Rodriguez-Berrocal, F.J.
Cell surface human alpha-L-fucosidase
Eur. J. Biochem.
268
3321-3331
2001
Chlorocebus aethiops, Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Merino-Trigo, A.; Rodriguez-Berrocal, F.J.; de Miguel, E.; Paez de la Cadena, M.
Activity and properties of alpha-L-fucosidase are dependent on the state of enterocytic differentiation of HT-29 colon cancer cells
Int. J. Biochem. Cell Biol.
34
1291-1303
2002
Homo sapiens
Manually annotated by BRENDA team
Khunsook, S.; Alhadeff, J.A.; Bean, B.S.
Purification and characterization of human seminal plasma alpha-L-fucosidase
Mol. Hum. Reprod.
8
221-227
2002
Homo sapiens
Manually annotated by BRENDA team
Blaser, A.; Reymond, J.L.
Stereoselective inhibition of alpha-L-fucosidases by N-benzyl aminocyclopentitols
Org. Lett.
2
1733-1736
2000
Bos taurus, Fusarium oxysporum, Homo sapiens
Manually annotated by BRENDA team
Wang, J.J.; Cao, E.H.
Rapid kinetic rate assay of the serum alpha-L-fucosidase in patients with hepatocellular carcinoma by using a novel substrate
Clin. Chim. Acta
347
103-109
2004
Homo sapiens
Manually annotated by BRENDA team
Li, C.; Qian, J.; Lin, J.S.
Purification and characterization of alpha-L-fucosidase from human primary hepatocarcinoma tissue
World J. Gastroenterol.
12
3770-3775
2006
Homo sapiens
Manually annotated by BRENDA team
Ho, C.W.; Lin, Y.N.; Chang, C.F.; Li, S.T.; Wu, Y.T.; Wu, C.Y.; Chang, C.F.; Liu, S.W.; Li, Y.K.; Lin, C.H.
Discovery of different types of inhibition between the human and thermotoga maritima alpha-fucosidases by fuconojirimycin-based derivatives
Biochemistry
45
5695-5702
2006
Thermotoga maritima, Homo sapiens (P04066)
Manually annotated by BRENDA team
Osanjo, G.; Dion, M.; Drone, J.; Solleux, C.; Tran, V.; Rabiller, C.; Tellier, C.
Directed evolution of the alpha-L-fucosidase from Thermotoga maritima into an alpha-L-transfucosidase
Biochemistry
46
1022-1033
2007
Thermotoga maritima, Thermotoga maritima (Q9WYE2), Homo sapiens (P04066)
Manually annotated by BRENDA team
Gu, G.; Liu, H.; Pinto, B.M.
Facile synthesis of sulfonium ion derivatives of 1,5-anhydro-5-thio-L-fucitol as potential alpha-L-fucosidase inhibitors
Carbohydr. Res.
341
2478-2486
2006
Homo sapiens
Manually annotated by BRENDA team
Intra, J.; Perotti, M.E.; Pavesi, G.; Horner, D.
Comparative and phylogenetic analysis of alpha-L-fucosidase genes
Gene
392
34-46
2007
Anopheles gambiae, Apis mellifera, Arabidopsis thaliana, Bacteroides fragilis (Q5L9F5), Bacteroides fragilis (Q5LAD6), Bacteroides fragilis (Q5LH32), Bacteroides fragilis (Q5LJ69), Bacteroides fragilis (Q64PP0), Bacteroides fragilis (Q64QR8), Bacteroides fragilis (Q64VK9), Bacteroides fragilis (Q64XY9), Bacteroides fragilis (Q650P6), Bacteroides thetaiotaomicron (Q8A1J6), Bacteroides thetaiotaomicron (Q8A6N5), Bos taurus, Caenorhabditis briggsae, Caenorhabditis elegans (P49713), Canis lupus familiaris (P48300), Caulobacter vibrioides (Q9AA16), Ciona intestinalis, Clostridium perfringens (Q8XNK9), Clostridium perfringens 13 (Q8XNK9), Cutibacterium acnes (Q6A632), Cutibacterium acnes (Q6AA53), Danio rerio (Q68EI7), Danio rerio (Q7ZW80), Dictyostelium discoideum (P10901), Drosophila ananassae, Drosophila erecta, Drosophila melanogaster, Drosophila mojavensis, Drosophila pseudoobscura, Drosophila simulans, Drosophila virilis, Drosophila yakuba, Gallus gallus, Homo sapiens (P04066), Homo sapiens (Q9BTY2), Homo sapiens, Macaca fascicularis (Q60HF8), Macaca mulatta, Microscilla sp. (Q93PB1), Microscilla sp. (Q93PB2), Microscilla sp. PRE1 (Q93PB1), Microscilla sp. PRE1 (Q93PB2), Monodelphis domestica, Mus musculus (Q99KR8), Mus musculus (Q99LJ1), Pan troglodytes, Porphyromonas gingivalis (Q7MTR2), Pyricularia oryzae 70-15, Rattus norvegicus (P17164), Rattus norvegicus (Q6AYS4), Rhodopirellula baltica (Q7UR90), Schistosoma japonicum (Q86FH9), Streptococcus gordonii (Q6QT50), Streptococcus pneumoniae (Q8CY64), Streptococcus pneumoniae TIGR4 (A0A0H2US78), Streptomyces coelicolor (Q9S2D4), Streptomyces coelicolor A3(2) (Q9S2D4), Streptomyces sp. (Q9Z4I9), Streptomyces sp. 142 (Q9Z4I9), Strongylocentrotus purpuratus, Takifugu rubripes, Tetraodon nigroviridis, Thermotoga maritima (Q9WYE2), Treponema denticola (Q73QG3), Xanthomonas axonopodis (Q8PI26), Xanthomonas campestris (A0A0H2X4Z5), Xenopus laevis (Q6IRC2), Xenopus tropicalis (Q5EAL9), Xenopus tropicalis (Q5M8I5)
Manually annotated by BRENDA team
Venditti, J.J.; Donigan, K.A.; Bean, B.S.
Crypticity and functional distribution of the membrane associated alpha-L-fucosidase of human sperm
Mol. Reprod. Dev.
74
758-766
2007
Homo sapiens
Manually annotated by BRENDA team
Liu, S.W.; Chen, C.S.; Chang, S.S.; Mong, K.K.; Lin, C.H.; Chang, C.W.; Tang, C.Y.; Li, Y.K.
Identification of essential residues of human alpha-L-fucosidase and tests of its mechanism
Biochemistry
48
110-120
2009
Homo sapiens (P04066), Homo sapiens, Dictyostelium discoideum (P10901), Pongo abelii (Q5RFI5), Schistosoma japonicum (Q86FH9), Halocynthia roretzi (Q8TA71), Drosophila melanogaster (Q9VTJ4), Thermotoga maritima (Q9WYE2)
Manually annotated by BRENDA team
Venditti, J.J.; Bean, B.S.
Stabilization of membrane-associated alpha-l-fucosidase by the human sperm equatorial segment
Int. J. Androl.
32
556-562
2008
Homo sapiens
Manually annotated by BRENDA team
Ali, S.; Jenkins, Y.; Kirkley, M.; Dagkalis, A.; Manivannan, A.; Crane, I.J.; Kirby, J.A.
Leukocyte extravasation: an immunoregulatory role for alpha-L-fucosidase?
J. Immunol.
181
2407-2413
2008
Homo sapiens
Manually annotated by BRENDA team
Matsumoto, K.; Shimizu, C.; Arao, T.; Andoh, M.; Katsumata, N.; Kohno, T.; Yonemori, K.; Koizumi, F.; Yokote, H.; Aogi, K.; Tamura, K.; Nishio, K.; Fujiwara, Y.
Identification of predictive biomarkers for response to trastuzumab using plasma FUCA activity and N-glycan identified by MALDI-TOF-MS
J. Proteome Res.
8
457-462
2009
Homo sapiens
Manually annotated by BRENDA team
Liu, T.W.; Ho, C.W.; Huang, H.H.; Chang, S.M.; Popat, S.D.; Wang, Y.T.; Wu, M.S.; Chen, Y.J.; Lin, C.H.
Role for alpha-L-fucosidase in the control of Helicobacter pylori-infected gastric cancer cells
Proc. Natl. Acad. Sci. USA
106
14581-14586
2009
Homo sapiens
Manually annotated by BRENDA team
Cheng, T.; Tu, S.; Chen, L.; Chen, M.; Chen, W.; Lin, Y.; Ho, C.; Lin, S.; Wu, C.; Ho, Y.
Down-regulation of ?-L-fucosidase 1 expression confers inferior survival for triple-negative breast cancer patients by modulating the glycosylation status of the tumor cell surface.
Oncotarget
6
21283-21300
2015
Homo sapiens (P04066)
Manually annotated by BRENDA team
Han, X.; Shokri Kojori, H.; Leblanc, R.; Kim, S.
Ultrasensitive plasmonic biosensors for real-time parallel detection of alpha-L-fucosidase and cardiac-troponin-I in whole human blood
Anal. Chem.
90
7795-7799
2018
Homo sapiens (P04066), Homo sapiens
Manually annotated by BRENDA team
Zhang, S.Y.; Lin, B.D.; Li, B.R.
Evaluation of the diagnostic value of alpha-L-fucosidase, alpha-fetoprotein and thymidine kinase 1 with ROC and logistic regression for hepatocellular carcinoma
FEBS open bio
5
240-244
2015
Homo sapiens (Q9BTY2), Homo sapiens
Manually annotated by BRENDA team