Information on EC 3.2.1.47 - galactosylgalactosylglucosylceramidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.47
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RECOMMENDED NAME
GeneOntology No.
galactosylgalactosylglucosylceramidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide + H2O = D-galactose + beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide
show the reaction diagram
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Glycosphingolipid biosynthesis - globo and isoglobo series
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SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactosyl-(1->4)-beta-D-galactosyl-(1->4)-beta-D-glucosyl-(1<->1)-ceramide galactohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
9023-01-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl-alpha-D-galactopyranoside + H2O
?
show the reaction diagram
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-
-
-
?
ceramide trihexoside + H2O
?
show the reaction diagram
D-galactosyl-D-galactosyl-D-glucosyl-N-acylsphingosine + H2O
lactosyl-N-acylsphingosine + D-galactose
show the reaction diagram
p-nitrophenyl-alpha-galactoside + H2O
p-nitrophenol + galactose
show the reaction diagram
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-
-
-
?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-galactosyl-D-galactosyl-D-glucosyl-N-acylsphingosine + H2O
lactosyl-N-acylsphingosine + D-galactose
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
NaCl
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activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ceramide lactoside
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-
ceramide trihexoside
D-myo-inositol
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weak competitive inhibitor
digalactosylceramide
EDTA
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-
galactose
glucosylsphingosine
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psychosine
Human serum albumin
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-
-
NaCl
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
digalactosylceramide
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in absence of sodium taurocholate and sodium chloride at low substrate concentrations
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.55
4-methylumbelliferyl-alpha-D-galactopyranoside
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-
0.05 - 0.15
ceramide trihexoside
6.7 - 9.3
p-nitrophenyl-alpha-galactoside
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-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0396
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-
0.076
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-
4.02
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-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5
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ceramide trihexoside
4.1
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D-galactosyl-D-galactosyl-D-glucosyl-N-acylsphingosine
4.5
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methylumbelliferyl alpha-galactoside
5.4
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form A
7.2
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form B
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 5.5
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pH 3.5 about 60% of maximal activity, pH 5.5 about 10% of maximal activity
3.5 - 6
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pH 6.0 about 10% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
24000
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4 * 24000, SDS-PAGE
95000
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sucrose density gradient centrifugation, gel filtration
103000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
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4 * 24000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
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contains sialic acid, multiple forms differ in sialic acid content
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.4
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above no activity
136495
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52
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1 h, 90% loss of activity
100
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A-1 75% loss of activity after boiling, A-2 100% loss of activity after boiling
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
human serum albumin stabilizes
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Butanol
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A1 retains enzymatic activity in 100% butanol, A2 retains activity in 20% butanol
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
0°C, 2 weeks
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5°C, at least 3 days, dialysed (NH4)2SO4 precipitates, at least 1 month
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE