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Information on EC 3.2.1.37 - xylan 1,4-beta-xylosidase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9FGY1

for references in articles please use BRENDA:EC3.2.1.37
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EC Tree
IUBMB Comments
Also hydrolyses xylobiose. Some other exoglycosidase activities have been found associated with this enzyme in sheep liver.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9FGY1
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
beta-xylosidase, alpha-l-arabinofuranosidase, xylosidase, beta-d-xylosidase, xyl i, xyl3a, xynb2, xln-dt, xynb3, axh-d3, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-arabinofuranosidase/beta-xylosidase
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1,4-beta-D-xylan xylohydrolase
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-
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beta-D-xylopyranosidase
-
-
-
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beta-D-xylosidase
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beta-xylosidase
-
-
-
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exo-1,4-beta-D-xylosidase
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-
-
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exo-1,4-beta-xylosidase
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-
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Exo-beta-(1,4)-xylanase
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-
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Xylan 1,4-beta-xylosidase
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-
-
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xylobiase
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-
-
-
additional information
cf. EC 3.2.1.55, alpha-L-arabinofuranosidase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
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PATHWAY SOURCE
PATHWAYS
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-
SYSTEMATIC NAME
IUBMB Comments
4-beta-D-xylan xylohydrolase
Also hydrolyses xylobiose. Some other exoglycosidase activities have been found associated with this enzyme in sheep liver.
CAS REGISTRY NUMBER
COMMENTARY hide
9025-53-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl alpha-L-arabinofuranoside + H2O
4-nitrophenol + alpha-L-arabinofuranose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
show the reaction diagram
-
-
-
?
xylobiose + H2O
2 beta-D-xylose
show the reaction diagram
-
-
-
?
xylotetraose + H2O
xylotriose + beta-D-xylose
show the reaction diagram
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
show the reaction diagram
-
-
-
?
xylobiose + H2O
2 beta-D-xylose
show the reaction diagram
-
-
-
?
xylotetraose + H2O
xylotriose + beta-D-xylose
show the reaction diagram
-
-
-
?
additional information
?
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.2
4-nitrophenyl-beta-D-xylopyranoside
pH 5.0, 37°C
0.7
4-nitrophenyl-beta-D-xylopyranoside
pH 5.0, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.35
4-nitrophenyl-beta-D-xylopyranoside
pH 5.0, 37°C
1.65
4-nitrophenyl-beta-D-xylopyranoside
pH 5.0, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
in T-DNA insertion mutant, mucilage release is both patchy and slow and seeds display delayed germination. Developmental analysis of mutant seeds reveals no change in mucilage secretory cell morphology, but changes in monosaccharide quantities are detected. Chemical and immunological analyses of mucilage extracted from bxl1 mutant seeds and antibody staining of developing seed coats reveal an increase in (1-5)-linked arabinans, suggesting that BXL1 is acting as an a-L-arabinofuranosidase in the seed coat
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BXL1_ARATH
774
1
83524
Swiss-Prot
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64000
x * 64000, SDS-PAGE
67000
x * 67000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 64000, SDS-PAGE
?
x * 67000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
binds to concanavalin A
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme from stem, 29.1fold by concanavalin A affinity chromatography, cation exchange chromatography, and gel filtration to homogeneity
native enzyme from stem, 40.3fold by concanavalin A affinity chromatography, cation exchange chromatography, and gel filtration to homogeneity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene xyl1, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis
gene xyl4, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Minic, Z.; Rihouey, C.; Do, C.T.; Lerouge, P.; Jouanin, L.
Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis
Plant Physiol.
135
867-878
2004
Arabidopsis thaliana (Q9FGY1), Arabidopsis thaliana (Q9FLG1)
Manually annotated by BRENDA team
Arsovski, A.A.; Popma, T.M.; Haughn, G.W.; Carpita, N.C.; McCann, M.C.; Western, T.L.
AtBXL1 encodes a bifunctional beta-D-xylosidase/alpha-L-arabinofuranosidase required for pectic arabinan modification in Arabidopsis mucilage secretory cells
Plant Physiol.
150
1219-1234
2009
Arabidopsis thaliana (Q9FGY1)
Manually annotated by BRENDA team