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Information on EC 3.2.1.35 - hyaluronoglucosaminidase and Organism(s) Sus scrofa and UniProt Accession Q6RHW4

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IUBMB Comments
Also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan.
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This record set is specific for:
Sus scrofa
UNIPROT: Q6RHW4
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Word Map
The taxonomic range for the selected organisms is: Sus scrofa
The enzyme appears in selected viruses and cellular organisms
Synonyms
chondroitinase, testicular hyaluronidase, hyal1, hyal2, ph-20, hyal-1, rhuph20, spreading factor, hyal-2, mgea5, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hyaluronidase
-
chondritinase I
-
-
-
-
chondroitinase
-
-
-
-
hyaluronidase
hyaluronoglucosidase
-
-
-
-
Mu toxin
-
-
-
-
Sperm surface protein PH-20
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
hyaluronate 4-glycanohydrolase
Also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan.
CAS REGISTRY NUMBER
COMMENTARY hide
37326-33-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
hyaluronan + 2 H2O
N-acetyl-beta-D-glucosamine + D-glucuronate + ?
show the reaction diagram
-
-
-
-
?
hyaluronic acid + H2O
?
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
hyaluronic acid + H2O
?
show the reaction diagram
-
-
-
-
?
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 8
-
55000 Da form
6 - 8
-
70000 and 80000 Da enzymes
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Hyal1; gene HYAL1
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
high expression
Manually annotated by BRENDA team
high expression
Manually annotated by BRENDA team
high expression
Manually annotated by BRENDA team
high expression in adult male testis, low expression in young male testis
Manually annotated by BRENDA team
-
-
Manually annotated by BRENDA team
high expression in adult male testis
Manually annotated by BRENDA team
additional information
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HYAL1_PIG
435
0
48507
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
-
SDS-PAGE, the 55000 Da enzyme form is active both at the acidic (pH 3.7) and the neutral pH (pH 7.4) and is able to bind to heparin
65000
-
SDS-PAGE, the 65000 Da enzyme form found in seminal vesicle fluids is active only at acidic pH and does not interact with heparin
70000
-
SDS-PAGE, the 70000 Da enzyme form is active only at neutral pH
80000
-
SDS-PAGE, the 80000 Da enzyme form is active only at neutral pH
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
heparin-Sepharose column chromatography
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
genes HYAL1, HYAL2, and HYAL3 are clustered on chromosome SSC13q21, DNA and amino acid sequence determination and analysis
genes HYAL1, HYAL2, and HYAL3 clustered on chromosome SSC13q21, DNA and amino acid sequence determination and analysis
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Meyer, K.; Rapport, M.M.
Hyaluronidases
Adv. Enzymol. Relat. Subj. Biochem.
13
199-236
1952
Bos taurus, Cavia porcellus, Homo sapiens, Ovis aries aries, Sus scrofa
Manually annotated by BRENDA team
Gatphayak, K.; Knorr, C.; Beck, J.; Brenig, B.
Molecular characterization of porcine hyaluronidase genes 1, 2, and 3 clustered on SSC13q21
Cytogenet. Genome Res.
109
535
2005
Sus scrofa (Q6RHW2), Sus scrofa (Q6RHW3), Sus scrofa (Q6RHW4)
-
Manually annotated by BRENDA team
Cibulkova, E.; Manaskova, P.; Jonakova, V.; Ticha, M.
Preliminary characterization of multiple hyaluronidase forms in boar reproductive tract
Theriogenology
68
1047-1054
2007
Sus scrofa
Manually annotated by BRENDA team