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Information on EC 3.2.1.35 - hyaluronoglucosaminidase and Organism(s) Homo sapiens and UniProt Accession O43820

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IUBMB Comments
Also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan.
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Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
UNIPROT: O43820
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Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Synonyms
chondroitinase, testicular hyaluronidase, hyal1, hyal2, ph-20, hyal-1, rhuph20, spreading factor, hyal-2, mgea5, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hyaluronidase
-
hyaluronidase 3
-
chondritinase I
-
-
-
-
chondroitin sulfate hydrolase
-
-
chondroitinase
-
-
-
-
Cumulase
-
human recombinant hyaluronidase
endo-alpha-N-acetyl-hexosaminidase
-
HPH-20
-
-
human PH-20
-
-
Hyal
-
-
Hyal-1
HYAL1
HYAL1-v1
alternatively spliced and enzymatically inactive variant of HYAL1
HYAL1-v3
-
isoform
Hyal2
HYAL3-v1
-
isoform
HYAL3-v2
-
isoform
HYAL4
-
-
hyaluronidase
hyaluronidase 1
-
hyaluronidase 2
hyaluronidase PH20
-
hyaluronidase-1
hyaluronidase-2
hyaluronidase-4
-
-
hyaluronoglucosaminidase 1
-
Hyaluronoglucosaminidase 1-type hyaluronidase
-
hyaluronoglucosaminidase 2
-
hyaluronoglucosidase
-
-
-
-
HYase
-
-
Mu toxin
-
-
-
-
PH-20
-
-
rHuPH20
-
-
SPAM1
-
-
Sperm surface protein PH-20
-
-
-
-
spreading factor
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Random hydrolysis of (1->4)-linkages between N-acetyl-beta-D-glucosamine and D-glucuronate residues in hyaluronate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
hyaluronate 4-glycanohydrolase
Also hydrolyses 1,4-beta-D-glycosidic linkages between N-acetyl-galactosamine or N-acetylgalactosamine sulfate and glucuronic acid in chondroitin, chondroitin 4- and 6-sulfates, and dermatan.
CAS REGISTRY NUMBER
COMMENTARY hide
37326-33-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
hyaluronan + H2O
?
show the reaction diagram
hyaluronic acid + H2O
?
show the reaction diagram
-
-
-
?
4-S-chondroitin sulfate + H2O
?
show the reaction diagram
-
-
-
?
6-S-chondroitin sulfate + H2O
?
show the reaction diagram
-
-
-
?
chondroitin sulfate + H2O
?
show the reaction diagram
-
-
-
-
?
chondroitin sulfate A + H2O
?
show the reaction diagram
-
poor substrate
-
-
?
chondroitin sulfate C + H2O
?
show the reaction diagram
-
-
-
-
?
chondroitin sulfate D + H2O
?
show the reaction diagram
-
-
-
-
?
hyaluronan + 2 H2O
N-acetyl-beta-D-glucosamine + D-glucuronate + ?
show the reaction diagram
hyaluronan + H2O
?
show the reaction diagram
hyaluronan + H2O
hyaluronan oligomers
show the reaction diagram
hyaluronan + H2O
tetraoligosaccharides + hexaoligosaccharides
show the reaction diagram
-
-
after complete digestion
?
hyaluronate + H2O
N-acetyl-beta-D-glucosamine + D-glucuronate + ?
show the reaction diagram
from umbilical cord
-
?
hyaluronic acid + H2O
?
show the reaction diagram
hyaluronic acid + H2O
hyaluronic acid oligomers
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
hyaluronan + H2O
?
show the reaction diagram
complete degradation, absolutely specific for
-
?
hyaluronan + 2 H2O
N-acetyl-beta-D-glucosamine + D-glucuronate + ?
show the reaction diagram
hyaluronan + H2O
?
show the reaction diagram
human platelets degrade the proinflammatory matrix hyaluronan through the activity of HYAL2 and platelet activation causes the immediate translocation of HYAL2 from a distinct population of alpha-granules to platelet surfaces where it exerts its catalytic activity
-
-
?
hyaluronan + H2O
hyaluronan oligomers
show the reaction diagram
-
the enzyme is required for fertility facilitating the penetration of sperm through the cumulus mass in the ovum
-
-
?
hyaluronic acid + H2O
?
show the reaction diagram
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
1.8fold increasing activity at 5 mM
Mg2+
-
1.8fold increasing activity at 5 mM
NaCl
-
-
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6-palmitoyl-L-ascorbic acid
-
0.005 mM, 93% inhibition at pH 7.0
chondroitin O-sulfate
dermatan O-sulfate
-
fully O-sulfonated chondroitin sulfate
-
competitive and noncompetitive mode, 50% inhibition at 0.00135 mg/ml
-
fully O-sulfonated dermatan sulfate
-
competitive and noncompetitive mode, 50% inhibition at 0.00133 mg/ml
fully O-sulfonated heparan sulfate
-
competitive and noncompetitive mode, 50% inhibition at 0.00128 mg/ml
-
fully O-sulfonated heparin
-
50% inhibition at 0.00114 mg/ml
-
fully O-sulfonated hyaluronan
-
50% inhibition at 0.00078 mg/ml
glutathione
-
non-competitive, complete inhibition at 1.8 mg/ml
glycyrrhizic acid
-
-
heparan O-sulfate
-
IC50 = 0.00128 mg/ml
-
heparan sulfate
-
43% inhibition at 500 ng/ml
heparin
heparin O-sulfate
-
IC50 = 0.00114 mg/ml, competitive and non-competitive inhibitory effects
-
Hg2+
-
moderate inhibition at 20 mM
hyaluronan O-sulfate
-
IC50 = 0.00078 mg/ml
L-Arginine-HCl
-
8% residual activity at 1 mM
L-ascorbic acid decanoate
-
-
L-ascorbic acid dodecanoate
-
-
L-ascorbic acid tridecanoate
-
-
L-ascorbic acid undecanoate
-
-
N-(4-chlorobenzyl)-1-(4-fluorobenzyl)-1H-indole-3-carboxamide
-
0.05 mM, 21% inhibition at pH 7.0
N-(4-fluorobenzyl)-1-benzyl-1H-indole-2-carboxamide
-
0.05 mM, 61% inhibition at pH 7.0
N-acetyl-L-cysteine
-
non-competitive, complete inhibition at 1.8 mg/ml
polymer from gentisic acid
-
-
-
quinol
-
irreversible inhibition
quinone
-
irreversible inhibition
additional information
-
the residual activities normalized to 100% at 0 concentrations of Terminalia chebula extract are reduced to 84% at 0.1 mg/ml, 68% at 0.5 mg/ml, 57% at 1 mg/ml, 26% at 1.5 mg/ml, 20% at 3 mg/ml, and 7% at 30 mg/ml
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
interleukin 1-beta
acts synergistically with TNF-alpha to increase gene expression and activity
-
TNF-alpha
acts synergistically with interleukin 1-beta to increase gene expression and activity
-
bovine serum albumin
-
interleukin 1-beta
acts synergistically with TNF-alpha to increase gene expression and activity
-
TNF-alpha
acts synergistically with interleukin 1-beta to increase gene expression and activity
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.038 - 0.367
hyaluronan
additional information
hyaluronan
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
hyaluronan
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.026
glycyrrhizic acid
Homo sapiens
-
at pH 3.5
0.378
L-ascorbic acid decanoate
Homo sapiens
-
at pH 3.5
0.076
L-ascorbic acid dodecanoate
Homo sapiens
-
at pH 3.5
0.05
L-ascorbic acid tridecanoate
Homo sapiens
-
at pH 3.5
0.143
L-ascorbic acid undecanoate
Homo sapiens
-
at pH 3.5
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.4
pH 3.5, 37Ā°C
0.08
pH 3.5, 37Ā°C
1.5
pH 7.1, 37Ā°C
1.55
pH 7.1, 37Ā°C
22.6
pH 3.5, 37Ā°C
61.15
pH 3.5, 37Ā°C
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.9
-
assay at
3.3 - 4
-
with 0.15 M NaCl
3.5 - 4
-
-
3.8
assay at
4
-
additional slight activity peak observed at pH 7.0
4.5
wild type enzyme
4.5 - 5
-
-
4.5 - 5.5
-
9.6
assay at
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
tissue and cells
Manually annotated by BRENDA team
Hyal-like activity is present in the apical and basolateral secretions from HBE cells where Hyals 1, 2, and 3 are expressed
Manually annotated by BRENDA team
increased expression and activity is observed in tracheal sections and in bronchoalveolar lavage (BAL) obtained from subjects with asthma when compared with normal lung donors and healthy volunteers
Manually annotated by BRENDA team
primary pilocytic
Manually annotated by BRENDA team
-
HPH-20
Manually annotated by BRENDA team
Hyal-like activity is present in the apical and basolateral secretions from HBE cells where Hyals 1, 2, and 3 are expressed
Manually annotated by BRENDA team
increased expression and activity is observed in tracheal sections and in bronchoalveolar lavage (BAL) obtained from subjects with asthma when compared with normal lung donors and healthy volunteers
Manually annotated by BRENDA team
-
HPH-20
Manually annotated by BRENDA team
contains only HYAL2 but not HYAL1
Manually annotated by BRENDA team
-
HPH-20
Manually annotated by BRENDA team
glioblastoma cell line
Manually annotated by BRENDA team
-
HPH-20
Manually annotated by BRENDA team
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
spermatid, HPH-20
-
Manually annotated by BRENDA team
130 kDa enzyme form
Manually annotated by BRENDA team
130 kDa enzyme form
Manually annotated by BRENDA team
75 kDa enzyme form
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
malfunction
patients with inflammatory bowel disease have lower platelet HYAL2 levels and activity than healthy controls
physiological function
human platelets degrade the proinflammatory matrix hyaluronan through the activity of HYAL2 and platelet activation causes the immediate translocation of HYAL2 from a distinct population of alpha-granules to platelet surfaces where it exerts its catalytic activity
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
HYAL3_HUMAN
417
0
46501
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
130000
x * 130000, enzyme form 1, SDS-PAGE, x * 75000, enzyme form 2, SDS-PAGE
45000
-
SDS-PAGE
47370
calculated from sequence of cDNA
48300
-
recombinant enzyme from Escherichia coli, SDS-PAGE
50870
MALDI-TOF mass spectrometry
52000
x * 52000, SDS-PAGE
54000
-
recombinant enzyme from Drosophila melanogaster Schneider-2 cells, SDS-PAGE
55000
-
SDS-PAGE
59000
-
SDS-PAGE
75000
x * 130000, enzyme form 1, SDS-PAGE, x * 75000, enzyme form 2, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
three-dimensional structure analysis and modeling of isozymes, structure-reaction mechanism relationship, overview
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
proteolytic modification
posttranslational modification of a larger protein of 130 kDa to a 75 kDa form
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapour diffusion method with mother liquor containing 1.5 M NaCl, 10% (v/v) ethanol, and 1/10 volume of 30% (w/v) galactose, at pH 4.6
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D129N
D146N
-
site-directed mutagenesis of HPH-20, the mutant shows highly reduced activity
DELTA351-end
putative structural mutant with a deleted EGF-like domain, significant decreased enzymatic activity
DELTA356-end
putative structural mutant with a deleted EGF-like domain, significant decreased enzymatic activity
E131Q
E148Q
-
site-directed mutagenesis of HPH-20, inactive mutant
L356tr
putative structural mutant, reduced activity
N216A
N350A
N350tr
putative structural mutant, reduced activity
R265L
S245A
Y202F
Y247F
additional information
-
expression as PH-20-autotransporter fusion protein for cell surface display in Escherichia coli F470, a strain missing the O-polysaccharide of LPS, yields cells with sufficient hyaluronidase activity
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.7
-
HAase activity is only detectable in acidic conditions of a pH 3.7 buffer
678378
4.9
-
no activity above
171395
5.1 - 5.9
human serum hyaluronidase 1 is inactive above pH 5.1, while human serum hyaluronidase 1 variant HYAL1DELTAL exhibits activity up to pH 5.9
704505
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant protein
ammonium sulfate precipitation and Ni-ion metal affinity chromatography
-
Chelating Sepharose Fast Flow column chromatography and Sephacryl S200 gel filtration
HiTrap Q column chromatography and Superdex 200 HR 10/30 gel filtration
-
Hyal2 is strongly associated with cell membrane fractions from which it can be extracted using a Triton X-114 treatment, but not an osmotic shock or an alkaline carbonate solution. Treatment of membrane preparations with phosphatidylinositol-specific phospholipase C releases immunoreactive Hyal2 into the aqueous phase. The protein is attached to the membrane through a functional GPI anchor. Hyal2 transfected in COS-7 cells is associated with detergent-resistant, cholesterol-rich membranes known as lipid rafts
partial
-
recombinant protein
to homogeneity, chromatography steps
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in human bronchial epithelial cell (HBE)
transient expression in Nicotiana benthamiana
DNA and amino acid sequence determination and analysis, in vitro translation
expressed in 22Rv1 human prostate adenomacarcinoma cells and in HEK-293 human embryonic kidney cells as a N-terminal FLAG fusion protein. Abundant soluble FLAG-tagged Hyal1 is secreted to the conditioned cell culture media of transfectants
expressed in COS-7 cells
-
expressed in Drosophila melanogaster Schneider 2 cells
expressed in Escherichia coli and in Xenopus laevis oocytes
expressed in Escherichia coli BL21(DE3)RIL cells and Drosophila melanogaster Schneider-2 cells
-
expressed in S-2 cells
-
expression in human bronchial epithelial cell (HBE)
gene mgea5, DNA sequence determination and analysis, genomic organization on chromosome 10, 2 splice variants, one missing a putative acetyltransferase domain of the enzyme
isozymes Hyal1-Hyal4, sequence comparison, the genes encoding isozymes Hyal1-Hyal3 are tightly clustered on chromosome 3q21.3, Hyal4 and HPH-20 are clustered on 7q31.3, expression profiles
-
transfection of COS-7 cell
transient expression in Nicotiana benthamiana
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
basic fibroblast growth factor decreases hyaluronidase-2 expression in HT-1080 cells
estrogen receptor(-) breast cancer cells secrete significantly more hyaluronidase than estrogen receptor(+) breast cancer cells
-
the median of isoform HYAL1 mRNA level is 69% lower in grade 3 serous ovarian cancers compared to normal ovaries
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
diagnostics
increased concentration of HYAL1-type enzyme correlates with tumour progression and is a marker for grade (G) 2 or 3 bladder cancer
medicine
molecular biology
-
the influence of hyaluronidase digestion modulates CD44 expression in various cancer cell lines
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Toida, T.; Ogita, Y.; Suzuki, A.; Toyoda, H.; Imanari, T.
Inhibition of hyaluronidase by fully O-sulfonated glycosaminoglycans
Arch. Biochem. Biophys.
370
176-182
1999
Homo sapiens
Manually annotated by BRENDA team
Maingonnat, C.; Victor, R.; Bertrand, P.; Courel, M.N.; Maunoury, R.; Delpech, B.
Activation and inhibition of human cancer cell hyaluronidase by proteins
Anal. Biochem.
268
30-34
1998
Homo sapiens
Manually annotated by BRENDA team
Schwartz, D.M.; Shuster, S.; Jumper, M.D.; Chang, A.; Stern, R.
Human vitreous hyaluronidase: isolation and characterization
Curr. Eye Res.
15
1156-1162
1996
Homo sapiens
Manually annotated by BRENDA team
Afify, A.M.; Stern, M.; Guntenhöner, M.; Stern, R.
Purification and characterization of human serum hyaluronidase
Arch. Biochem. Biophys.
305
434-441
1993
Homo sapiens
Manually annotated by BRENDA team
Meyer, K.; Rapport, M.M.
Hyaluronidases
Adv. Enzymol. Relat. Subj. Biochem.
13
199-236
1952
Bos taurus, Cavia porcellus, Homo sapiens, Ovis aries aries, Sus scrofa
Manually annotated by BRENDA team
Takahashi, T.; Ikegami-Kawai, M.; Okuda, R.; Suzuki, K.
A fluorometric Morgan-Elson method for hyaluronidase activity
Anal. Biochem.
322
257-263
2003
Bos taurus, Oryctolagus cuniculus, Homo sapiens
Manually annotated by BRENDA team
Comtesse, N.; Maldener, E.; Meese, E.
Identification of a nuclear variant of MGEA5, a cytoplasmic hyaluronidase and a beta-N-acetylglucosaminidase
Biochem. Biophys. Res. Commun.
283
634-640
2001
Homo sapiens (O60502), Homo sapiens
Manually annotated by BRENDA team
Lokeshwar, V.B.; Schroeder, G.L.; Carey, R.I.; Soloway, M.S.; Iida, N.
Regulation of hyaluronidase activity by alternative mRNA splicing
J. Biol. Chem.
277
33654-33663
2002
Homo sapiens, Homo sapiens (O43820), Homo sapiens (Q12794)
Manually annotated by BRENDA team
Stern, R.; Jedrzejas, M.J.
Hyaluronidases: their genomics, structures, and mechanisms of action
Chem. Rev.
106
818-839
2006
Bos taurus, Homo sapiens, Mus musculus, Apis mellifera (Q08169)
Manually annotated by BRENDA team
Chao, K.L.; Muthukumar, L.; Herzberg, O.
Structure of human hyaluronidase-1, a hyaluronan hydrolyzing enzyme involved in tumor growth and angiogenesis
Biochemistry
46
6911-6920
2007
Homo sapiens (Q12794), Homo sapiens
Manually annotated by BRENDA team
Christopoulos, T.A.; Papageorgakopoulou, N.; Theocharis, D.A.; Mastronikolis, N.S.; Papadas, T.A.; Vynios, D.H.
Hyaluronidase and CD44 hyaluronan receptor expression in squamous cell laryngeal carcinoma
Biochim. Biophys. Acta
1760
1039-1045
2006
Homo sapiens
Manually annotated by BRENDA team
Lokeshwar, V.B.; Estrella, V.; Lopez, L.; Kramer, M.; Gomez, P.; Soloway, M.S.; Lokeshwar, B.L.
HYAL1-v1, an alternatively spliced variant of HYAL1 hyaluronidase: a negative regulator of bladder cancer
Cancer Res.
66
11219-11227
2006
Homo sapiens (Q12794)
Manually annotated by BRENDA team
Hofinger, E.S.; Spickenreither, M.; Oschmann, J.; Bernhardt, G.; Rudolph, R.; Buschauer, A.
Recombinant human hyaluronidase Hyal-1: insect cells versus Escherichia coli as expression system and identification of low molecular weight inhibitors
Glycobiology
17
444-453
2007
Homo sapiens
Manually annotated by BRENDA team
Vigdorovich, V.; Miller, A.D.; Strong, R.K.
Ability of hyaluronidase 2 to degrade extracellular hyaluronan is not required for its function as a receptor for Jaagsiekte sheep retrovirus
J. Virol.
81
3124-3129
2007
Homo sapiens
Manually annotated by BRENDA team
Monzon, M.E.; Manzanares, D.; Schmid, N.; Casalino-Matsuda, S.M.; Forteza, R.M.
Hyaluronidase expression and activity is regulated by pro-inflammatory cytokines in human airway epithelial cells
Am. J. Respir. Cell Mol. Biol.
39
289-295
2008
Homo sapiens (O43820), Homo sapiens (Q12794), Homo sapiens (Q12891), Homo sapiens
Manually annotated by BRENDA team
Ganesh, S.; Gonzalez-Edick, M.; Gibbons, D.; Van Roey, M.; Jooss, K.
Intratumoral coadministration of hyaluronidase enzyme and oncolytic adenoviruses enhances virus potency in metastatic tumor models
Clin. Cancer Res.
14
3933-3941
2008
Homo sapiens
Manually annotated by BRENDA team
Zhang, L.; Bharadwaj, A.G.; Casper, A.; Barkley, J.; Barycki, J.J.; Simpson, M.A.
Hyaluronidase activity of human hyal1 requires active site acidic and tyrosine residues
J. Biol. Chem.
284
9433-9442
2009
Homo sapiens, Homo sapiens (Q12794)
Manually annotated by BRENDA team
Wang, F.; Grigorieva, E.V.; Li, J.; Senchenko, V.N.; Pavlova, T.V.; Anedchenko, E.A.; Kudryavtseva, A.V.; Tsimanis, A.; Angeloni, D.; Lerman, M.I.; Kashuba, V.I.; Klein, G.; Zabarovsky, E.R.
HYAL1 and HYAL2 inhibit tumour growth in vivo but not in vitro
PLoS ONE
3
e3031
2008
Homo sapiens (Q12794), Homo sapiens (Q12891), Homo sapiens
Manually annotated by BRENDA team
Kramer, M.W.; Golshani, R.; Merseburger, A.S.; Knapp, J.; Garcia, A.; Hennenlotter, J.; Duncan, R.C.; Soloway, M.S.; Jorda, M.; Kuczyk, M.A.; Stenzl, A.; Lokeshwar, V.B.
HYAL-1 Hyaluronidase: A Potential Prognostic Indicator for Progression to Muscle Invasion and Recurrence in Bladder Cancer
Eur. Urol.
57
86-93
2010
Homo sapiens (Q12794), Homo sapiens
Manually annotated by BRENDA team
Sharma, R.; Mahadeswaraswamy, Y.H.; Harish Kumar, K.; Devaraja, S.; Kemparaju, K.; Vishwanath, B.S.; Girish, K.S.
Effect of anticoagulants on the plasma hyaluronidase activities
J. Clin. Lab. Anal.
23
29-33
2009
Homo sapiens (Q12794)
Manually annotated by BRENDA team
Stern, R.
Hyaluronidases in cancer biology
Semin. Cancer Biol.
18
275-280
2008
Homo sapiens
Manually annotated by BRENDA team
de la Motte, C.; Nigro, J.; Vasanji, A.; Rho, H.; Kessler, S.; Bandyopadhyay, S.; Danese, S.; Fiocchi, C.; Stern, R.
Platelet-derived hyaluronidase 2 cleaves hyaluronan into fragments that trigger monocyte-mediated production of proinflammatory cytokines
Am. J. Pathol.
174
2254-2264
2009
Mus musculus (O35632), Homo sapiens (Q12891)
Manually annotated by BRENDA team
Kim, J.W.; Kim, J.H.; Chung, S.J.; Chung, B.H.
An operationally simple colorimetric assay of hyaluronidase activity using cationic gold nanoparticles
Analyst
134
1291-1293
2009
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Berdiaki, A.; Nikitovic, D.; Tsatsakis, A.; Katonis, P.; Karamanos, N.K.; Tzanakakis, G.N.
bFGF induces changes in hyaluronan synthase and hyaluronidase isoform expression and modulates the migration capacity of fibrosarcoma cells
Biochim. Biophys. Acta
1790
1258-1265
2009
Homo sapiens (Q12794), Homo sapiens (Q12891)
Manually annotated by BRENDA team
Nykopp, T.; Rilla, K.; Sironen, R.; Tammi, M.; Tammi, R.; Hämäläinen, K.; Heikkinen, A.; Komulainen, M.; Kosma, V.; Anttila, M.
Expression of hyaluronan synthases (HAS1-3) and hyaluronidases (HYAL1-2) in serous ovarian carcinomas: Inverse correlation between HYAL1 and hyaluronan content
BMC Cancer
9
143
2009
Homo sapiens (Q12794), Homo sapiens (Q12891)
Manually annotated by BRENDA team
Wang, X.Y.; Tan, J.X.; Vasse, M.; Delpech, B.; Ren, G.S.
Comparison of hyaluronidase expression, invasiveness and tubule formation promotion in ER (-) and ER (+) breast cancer cell lines in vitro
Chin. Med. Sci.
122
1300-1304
2009
Homo sapiens
Manually annotated by BRENDA team
Colombo, R.
Editorial Comment on: HYAL-1 hyaluronidase: A potential prognostic indicator for progression to muscle invasion and recurrence in bladder cancer
Eur. Urol.
57
93-94
2010
Homo sapiens (Q12794)
Manually annotated by BRENDA team
Reitinger, S.; Muellegger, J.; Greiderer, B.; Nielsen, J.E.; Lepperdinger, G.
Designed human serum hyaluronidase 1 variant, HYAL1DeltaL, exhibits activity up to pH 5.9
J. Biol. Chem.
284
19173-19177
2009
Homo sapiens (Q12794), Homo sapiens
Manually annotated by BRENDA team
Evison, M.; Pretty, C.; Taylor, E.; Franklin, C.
Human recombinant hyaluronidase (Cumulase) improves intracytoplasmic sperm injection survival and fertilization rates
Reprod. Biomed. Online
18
811-814
2009
Homo sapiens
Manually annotated by BRENDA team
Srivastav, A.; Chandra, A.; Singh, M.; Jamal, F.; Rastogi, P.; Rajendran, S.M.; Bansode, F.W.; Lakshmi, V.
Inhibition of hyaluronidase activity of human and rat spermatozoa in vitro and antispermatogenic activity in rats in vivo by Terminalia chebula, a flavonoid rich plant
Reprod. Toxicol.
29
214-224
2010
Homo sapiens, Rattus norvegicus
Manually annotated by BRENDA team
de Sa, V.; Canavez, F.; Silva, I.; Srougi, M.; Leite, K.
Isoforms of hyaluronidases can be a predictor of a prostate cancer of good prognosis
Urol. Oncol.
27
377-381
2009
Homo sapiens
Manually annotated by BRENDA team
Andre, B.; Duterme, C.; Van Moer, K.; Mertens-Strijthagen, J.; Jadot, M.; Flamion, B.
Hyal2 is a glycosylphosphatidylinositol-anchored, lipid raft-associated hyaluronidase
Biochem. Biophys. Res. Commun.
411
175-179
2011
Homo sapiens (Q12891)
Manually annotated by BRENDA team
Kaessler, A.; Olgen, S.; Jose, J.
Autodisplay of catalytically active human hyaluronidase hPH-20 and testing of enzyme inhibitors
Eur. J. Pharm. Sci.
42
138-147
2011
Bos taurus, Homo sapiens
Manually annotated by BRENDA team
Jung, Y.; Jung, M.Y.; Park, J.H.; Jung, G.C.; Hong, Y.S.; Yeom, C.H.; Lee, S.
Production of human hyaluronidase in a plant-derived protein expression system: plant-based transient production of active human hyaluronidase
Protein Expr. Purif.
74
181-188
2010
Homo sapiens (O43820), Homo sapiens (Q12794), Homo sapiens (Q12891), Homo sapiens (Q2M3T9), Homo sapiens
Manually annotated by BRENDA team
Sunitha, K.; Suresh, P.; Santhosh, M.S.; Hemshekhar, M.; Thushara, R.M.; Marathe, G.K.; Thirunavukkarasu, C.; Kemparaju, K.; Kumar, M.S.; Girish, K.S.
Inhibition of hyaluronidase by N-acetyl cysteine and glutathione: role of thiol group in hyaluronan protection
Int. J. Biol. Macromol.
55
39-46
2013
Bos taurus, Echis carinatus, Homo sapiens, Apis mellifera (Q08169)
Manually annotated by BRENDA team
Goto, Y.; Niwa, Y.; Suzuki, T.; Dohmae, N.; Umezawa, K.; Simizu, S.
C-mannosylation of human hyaluronidase 1: possible roles for secretion and enzymatic activity
Int. J. Oncol.
45
344-350
2014
Homo sapiens
Manually annotated by BRENDA team
Kaneiwa, T.; Miyazaki, A.; Kogawa, R.; Mizumoto, S.; Sugahara, K.; Yamada, S.
Identification of amino acid residues required for the substrate specificity of human and mouse chondroitin sulfate hydrolase (conventional hyaluronidase-4)
J. Biol. Chem.
287
42119-42128
2012
Homo sapiens, Mus musculus
Manually annotated by BRENDA team
Fang, S.; Hays Putnam, A.M.; LaBarre, M.J.
Kinetic investigation of recombinant human hyaluronidase PH20 on hyaluronic acid
Anal. Biochem.
480
74-81
2015
Homo sapiens (P38567), Homo sapiens
Manually annotated by BRENDA team
Albeiroti, S.; Ayasoufi, K.; Hill, D.; Shen, B.; De La Motte, C.
Platelet hyaluronidase-2 An enzyme that translocates to the surface upon activation to function in extracellular matrix degradation
Blood
125
1460-1469
2015
Homo sapiens (Q12891), Homo sapiens
Manually annotated by BRENDA team