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Information on EC 3.2.1.23 - beta-galactosidase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9MAJ7

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EC Tree
IUBMB Comments
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q9MAJ7
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The enzyme appears in selected viruses and cellular organisms
Synonyms
beta-galactosidase, beta-gal, beta-d-galactosidase, senescence-associated beta-galactosidase, endo-beta-galactosidase, bglap, sa-beta-gal, acid beta-galactosidase, beta galactosidase, betagal, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Acid beta-galactosidase
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-
-
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beta-D-galactoside galactohydrolase
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-
-
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beta-D-glactanase
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-
-
-
beta-D-lactosidase
-
-
-
-
beta-galase
-
-
-
-
beta-lactosidase
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-
-
-
driselase
-
-
-
-
Exo-(1-->4)-beta-D-galactanase
-
-
-
-
exo-beta-(1->3)-D-galactanase
-
-
-
-
galactosidase, beta
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-
-
-
Hydrolact
-
-
-
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lactase
-
-
-
-
lactosylceramidase II
-
-
-
-
Lactozym
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-
-
-
Maxilact
-
-
-
-
Oryzatym
-
-
-
-
p-nitrophenyl beta-galactosidase
-
-
-
-
S 2107
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-
-
-
SR12 protein
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-
-
-
Sumiklat
-
-
-
-
Trilactase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-D-galactoside galactohydrolase
Some enzymes in this group hydrolyse alpha-L-arabinosides; some animal enzymes also hydrolyse beta-D-fucosides and beta-D-glucosides; cf. EC 3.2.1.108 lactase.
CAS REGISTRY NUMBER
COMMENTARY hide
9031-11-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
show the reaction diagram
-
61% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
show the reaction diagram
-
18% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
show the reaction diagram
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + D-galactose
show the reaction diagram
-
43% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + D-galactose
show the reaction diagram
-
22% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + D-galactose
show the reaction diagram
-
72% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
4-methylumbelliferyl-beta-D-galactoside + H2O
4-methylumbelliferone + beta-D-galactose
show the reaction diagram
-
20% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + D-galactose
show the reaction diagram
-
-
-
?
5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside + H2O
5-bromo-4-chloroindol + D-galactose
show the reaction diagram
-
56% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
6-bromo-2-naphthyl-beta-galactoside + H2O
6-bromo-2-naphthol + D-galactose
show the reaction diagram
-
30% of the activity with 4-nitrophenyl-beta-D-galactopyranoside
-
?
a beta-D-galactoside + H2O
D-galactose + an alcohol
show the reaction diagram
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
a beta-D-galactoside + H2O
D-galactose + an alcohol
show the reaction diagram
-
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.1
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
associated with the cell wall
Manually annotated by BRENDA team
associated with the cell wall
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
BGAL5_ARATH
732
1
81444
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
x * 79000, calculated, x * 75000, SDS-PAGE
79000
x * 79000, calculated, x * 75000, SDS-PAGE
119544
x * 119544, calculated from amino acid sequence
75000
x * 81000, calculated, x * 75000, SDS-PAGE
81000
x * 81000, calculated, x * 75000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 79000, calculated, x * 75000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Pichia pastoris
expression in Pichia pastoris
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Gantulga, D.; Turan, Y.; Bevan, D.R.; Esen, A.
The Arabidopsis At1g45130 and At3g52840 genes encode beta-galactosidases with activity toward cell wall polysaccharides
Phytochemistry
69
1661-1670
2008
Arabidopsis thaliana (Q9LFA6), Arabidopsis thaliana (Q9MAJ7)
Manually annotated by BRENDA team
Seddigh, S.; Darabi, M.
Comprehensive analysis of beta-galactosidase protein in plants based on Arabidopsis thaliana
Turk. J. Biol.
38
140-150
2014
Asparagus officinalis, Persea americana, Musa acuminata, Brassica oleracea, Capsicum annuum, Carica papaya, Ricinus communis, Cicer arietinum, Citrus medica, Citrus sinensis, Coffea arabica, Dianthus caryophyllus, Drosophila melanogaster, Escherichia coli, Ficus carica, Fragaria x ananassa, Glycine max, Gossypium barbadense, Gossypium hirsutum, Homo sapiens, Hordeum vulgare, Linum usitatissimum, Lupinus angustifolius, Solanum lycopersicum, Mangifera indica, Malus domestica, Medicago truncatula, Mus musculus, Narcissus pseudonarcissus, Nicotiana tabacum, Pyrus communis, Petunia x hybrida, Vigna radiata, Physcomitrium patens, Populus trichocarpa, Prunus armeniaca, Prunus persica, Raphanus sativus, Sorghum bicolor, Trifolium pratense, Triticum monococcum, Vitis vinifera, Zea mays, Diospyros kaki, Oryza sativa Japonica Group, Picea sitchensis, Hymenaea courbaril, Cucumis melo var. cantalupo, Selaginella moellendorffii, Fragaria chiloensis, Prunus salicina, Actinidia deliciosa var. deliciosa, Gossypium raimondii, Eutrema halophilum, Gossypium herbaceum subsp. africanum, Sandersonia aurantiaca, Ziziphus jujuba, Arabidopsis thaliana (F4JUE3)
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Manually annotated by BRENDA team