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Information on EC 3.2.1.22 - alpha-galactosidase and Organism(s) Bacteroides thetaiotaomicron and UniProt Accession Q8A6L0

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IUBMB Comments
Also hydrolyses alpha-D-fucosides.
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This record set is specific for:
Bacteroides thetaiotaomicron
UNIPROT: Q8A6L0
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The taxonomic range for the selected organisms is: Bacteroides thetaiotaomicron
The enzyme appears in selected viruses and cellular organisms
Synonyms
alpha-galactosidase, alpha-galactosidase a, alpha-gal, alpha-gal a, agalsidase alfa, genzyme, alpha-d-galactosidase, a-galactosidase, fabrazyme, alpha-galactosidase i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
retaining alpha-galactosidase
-
Agalsidase alfa
-
-
-
-
alpha-D-galactosidase
-
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
-
-
alpha-galactosidase A
-
-
-
-
alpha-galactoside galactohydrolase
-
-
-
-
ceramidase, galactosylgalactosylglucosyl-
-
-
-
-
ceramide trihexosidase
-
-
-
-
ceramidetrihexosidase
-
-
-
-
ceramidetrihexoside-alpha-galactosidase
-
-
-
-
melibiase
-
-
-
-
trihexosyl ceramide galactosidase
-
-
-
-
trihexosylceramide alpha-galactosidase
-
-
-
-
trihexosylceramidealpha-galactosidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactoside galactohydrolase
Also hydrolyses alpha-D-fucosides.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-01-2
-
9025-35-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-azido-1-deoxy-beta-D-galactopyranoside + H2O
?
show the reaction diagram
-
-
-
?
4-nitrophenyl alpha-D-galactoside + H2O
4-nitrophenol + alpha-D-galactose
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.19 - 0.32
4-nitrophenyl alpha-D-galactoside
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00218 - 244
4-nitrophenyl alpha-D-galactoside
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0115 - 778
4-nitrophenyl alpha-D-galactoside
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC 29148
Uniprot
Manually annotated by BRENDA team
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 100 mM MES-NaOH buffer, pH 6.2, containing 12% (w/v) PEG (polyethylene glycol) 6000 or 100 mM MES-NaOH buffer containing 10% (w/v) PEG 10000, at 20°C
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D415N
the catalytic activity of the mutant enzyme E470Q is significantly decreased (kcat is 100000 lower than that of the wild type enzyme whereas the Km values of both D415N and wild type enzymes are roughly similar.)
E470Q
the catalytic activity of the mutant enzyme E470Q is significantly decreased (kcat is 28000 lower than that of the wild type enzyme whereas the Km values of both D415N and wild type enzymes are the same)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-chelating Sepharose column chromatography and DEAE-Sepharose column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli MV1184 cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Okuyama, M.; Kitamura, M.; Hondoh, H.; Kang, M.S.; Mori, H.; Kimura, A.; Tanaka, I.; Yao, M.
Catalytic mechanism of retaining alpha-galactosidase belonging to glycoside hydrolase family 97
J. Mol. Biol.
392
1232-1241
2009
Bacteroides thetaiotaomicron (Q8A6L0), Bacteroides thetaiotaomicron
Manually annotated by BRENDA team