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Information on EC 3.2.1.22 - alpha-galactosidase and Organism(s) Umbelopsis vinacea and UniProt Accession Q02402

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IUBMB Comments
Also hydrolyses alpha-D-fucosides.
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This record set is specific for:
Umbelopsis vinacea
UNIPROT: Q02402
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Word Map
The taxonomic range for the selected organisms is: Umbelopsis vinacea
The enzyme appears in selected viruses and cellular organisms
Synonyms
alpha-galactosidase, alpha-galactosidase a, alpha-gal, alpha-gal a, agalsidase alfa, genzyme, alpha-d-galactosidase, a-galactosidase, fabrazyme, alpha-galactosidase i, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
alpha-galactosidase I
-
Agalsidase alfa
-
-
-
-
alpha-D-galactosidase
-
-
-
-
Alpha-D-galactoside galactohydrolase
-
-
-
-
alpha-galactosidase A
-
-
-
-
alpha-galactoside galactohydrolase
-
-
-
-
ceramidase, galactosylgalactosylglucosyl-
-
-
-
-
ceramide trihexosidase
-
-
-
-
ceramidetrihexosidase
-
-
-
-
ceramidetrihexoside-alpha-galactosidase
-
-
-
-
melibiase
-
-
-
-
trihexosyl ceramide galactosidase
-
-
-
-
trihexosylceramide alpha-galactosidase
-
-
-
-
trihexosylceramidealpha-galactosidase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-D-galactoside galactohydrolase
Also hydrolyses alpha-D-fucosides.
CAS REGISTRY NUMBER
COMMENTARY hide
9023-01-2
-
9025-35-8
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
melibiose + H2O
D-galactose + D-glucose
show the reaction diagram
raffinose + H2O
sucrose + D-galactose
show the reaction diagram
-
-
-
-
?
stachyose + H2O
?
show the reaction diagram
-
-
-
-
?
sucrose + H2O
D-glucose + D-fructose
show the reaction diagram
-
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
28.3
-
alpha-galactosidase II
68.7
-
alpha-galactosidase I
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 4
-
alpha-galactosidase II
3 - 5
-
raffinose as substrate
4 - 6
-
stachyose or o-nitrophenyl alpha-D-galactoside as substrate
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
-
alpha-galactosidase II
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
synonym Mortierella vinacea
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q02402_9FUNG
417
0
46393
TrEMBL
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
240000
gel filtration
150000 - 200000
-
alpha-galactosidase II, calculated from cDNA
230000 - 270000
-
alpha-galactosidase I, calculated from cDNA
60000
-
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
x-ray crystallography
monomer
-
alpha-galactosidase II
tetramer
-
alpha-galactosidase I
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
-
2.7% D-glucosamine and 10.8% hexoses
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
0.01% bovine serum albumin stabilizes enzyme stability
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-15°C, no decrease in activity
-
4°C, 6 months, loss of activity less then 10%
-
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Saccharomyces cerevisiae
-
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nutrition
-
beet sugar refining
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Dey, P.M.; Pridham, J.B.
Biochemistry of alpha-galactosidases
Adv. Enzymol. Relat. Areas Mol. Biol.
36
91-130
1972
Acer pseudoplatanus, Klebsiella aerogenes, Aspergillus niger, Aspergillus paxillus, Bos taurus, Brassica oleracea, Vicia faba, Cajanus indicus, Calvatia cyanthiformis, Canavalia ensiformis, Ricinus communis, Citrullus lanatus subsp. vulgaris, Coffea sp., Cyamopsis tetragonoloba, Streptococcus pneumoniae, Escherichia coli, Epidinium ecaudatum, Eudiplodinium maggii, Helianthus annuus, Helix pomatia, Hordeum sp., Medicago sativa, Melolontha melolontha, Umbelopsis vinacea, Ophryoscolex caudatus, Vigna radiata var. radiata, Phaseolus vulgaris, Pisum sativum, Plantago ovata, Polyplastron multivesiculatum, Prunus dulcis, Raphanus sativus, Rattus norvegicus, Saccharomyces sp., Spinacia oleracea, Streptococcus equinus, Streptomyces olivaceus, Sus scrofa, Tritrichomonas suis, Trigonella foenum-graecum, Triticum sp., Vicia dumetorum, Vicia sativa
Manually annotated by BRENDA team
Crueger, A.; Crueger, W.
Carbohydrates
Biotechnology (Kieslich, K. , ed. ) Verlag Chemie, Weinheim
6A
421-457
1984
Circinella muscae, Umbelopsis vinacea, Streptomyces olivaceus
-
Manually annotated by BRENDA team
Shibuya, H.; Kobayashi, H.; Sato, T.; Kim, W.S.; Yoshida, S.; Kaneko, S.; Kasamo, K.; Kusakabe, I.
Purification, characterization and cDNA cloning of a novel alpha-Galactosidase from Mortierella vinacea
Biosci. Biotechnol. Biochem.
61
592-598
1997
Umbelopsis vinacea
Manually annotated by BRENDA team
Shibuya, H.; Kobayashi, H.; Yoshida, S.; Kaneko, S.; Park, G.G.; Kusakabe, I.
Purification and characterization of recombinant Mortierella vinacea alpha-galactosidase I and II expressed in Saccharomyces cerevisiae
Biosci. Biotechnol. Biochem.
63
1096-1099
1999
Umbelopsis vinacea
Manually annotated by BRENDA team
Fujimoto, Z.; Kaneko, S.; Kim, W.D.; Park, G.G.; Momma, M.; Kobayashi, H.
The tetramer structure of the glycoside hydrolase family 27 alpha-galactosidase I from Umbelopsis vinacea
Biosci. Biotechnol. Biochem.
73
2360-2364
2009
Umbelopsis vinacea (Q02402), Umbelopsis vinacea
Manually annotated by BRENDA team