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EC Tree
The taxonomic range for the selected organisms is: Umbelopsis vinacea The enzyme appears in selected viruses and cellular organisms
Synonyms
alpha-galactosidase, alpha-galactosidase a, alpha-gal, alpha-gal a, agalsidase alfa, genzyme, alpha-d-galactosidase, a-galactosidase, fabrazyme, alpha-galactosidase i,
more
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alpha-D-galactosidase
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Alpha-D-galactoside galactohydrolase
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alpha-galactosidase A
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alpha-galactoside galactohydrolase
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ceramidase, galactosylgalactosylglucosyl-
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ceramide trihexosidase
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ceramidetrihexosidase
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ceramidetrihexoside-alpha-galactosidase
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trihexosyl ceramide galactosidase
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trihexosylceramide alpha-galactosidase
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trihexosylceramidealpha-galactosidase
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hydrolysis of O-glycosyl bond
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alpha-D-galactoside galactohydrolase
Also hydrolyses alpha-D-fucosides.
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melibiose + H2O
D-galactose + D-glucose
raffinose + H2O
sucrose + D-galactose
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?
stachyose + H2O
?
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?
sucrose + H2O
D-glucose + D-fructose
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?
melibiose + H2O
D-galactose + D-glucose
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?
melibiose + H2O
D-galactose + D-glucose
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?
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28.3
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alpha-galactosidase II
68.7
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alpha-galactosidase I
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3 - 4
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alpha-galactosidase II
3 - 5
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raffinose as substrate
4 - 6
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stachyose or o-nitrophenyl alpha-D-galactoside as substrate
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60
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alpha-galactosidase II
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synonym Mortierella vinacea
UniProt
brenda
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brenda
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Q02402_9FUNG
417
0
46393
TrEMBL
Secretory Pathway (Reliability: 1 )
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150000 - 200000
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alpha-galactosidase II, calculated from cDNA
230000 - 270000
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alpha-galactosidase I, calculated from cDNA
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tetramer
x-ray crystallography
monomer
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alpha-galactosidase II
tetramer
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alpha-galactosidase I
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glycoprotein
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2.7% D-glucosamine and 10.8% hexoses
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0.01% bovine serum albumin stabilizes enzyme stability
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-15°C, no decrease in activity
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4°C, 6 months, loss of activity less then 10%
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expression in Saccharomyces cerevisiae
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nutrition
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beet sugar refining
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Dey, P.M.; Pridham, J.B.
Biochemistry of alpha-galactosidases
Adv. Enzymol. Relat. Areas Mol. Biol.
36
91-130
1972
Acer pseudoplatanus, Klebsiella aerogenes, Aspergillus niger, Aspergillus paxillus, Bos taurus, Brassica oleracea, Vicia faba, Cajanus indicus, Calvatia cyanthiformis, Canavalia ensiformis, Ricinus communis, Citrullus lanatus subsp. vulgaris, Coffea sp., Cyamopsis tetragonoloba, Streptococcus pneumoniae, Escherichia coli, Epidinium ecaudatum, Eudiplodinium maggii, Helianthus annuus, Helix pomatia, Hordeum sp., Medicago sativa, Melolontha melolontha, Umbelopsis vinacea, Ophryoscolex caudatus, Vigna radiata var. radiata, Phaseolus vulgaris, Pisum sativum, Plantago ovata, Polyplastron multivesiculatum, Prunus dulcis, Raphanus sativus, Rattus norvegicus, Saccharomyces sp., Spinacia oleracea, Streptococcus equinus, Streptomyces olivaceus, Sus scrofa, Tritrichomonas suis, Trigonella foenum-graecum, Triticum sp., Vicia dumetorum, Vicia sativa
brenda
Crueger, A.; Crueger, W.
Carbohydrates
Biotechnology (Kieslich, K. , ed. ) Verlag Chemie, Weinheim
6A
421-457
1984
Circinella muscae, Umbelopsis vinacea, Streptomyces olivaceus
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brenda
Shibuya, H.; Kobayashi, H.; Sato, T.; Kim, W.S.; Yoshida, S.; Kaneko, S.; Kasamo, K.; Kusakabe, I.
Purification, characterization and cDNA cloning of a novel alpha-Galactosidase from Mortierella vinacea
Biosci. Biotechnol. Biochem.
61
592-598
1997
Umbelopsis vinacea
brenda
Shibuya, H.; Kobayashi, H.; Yoshida, S.; Kaneko, S.; Park, G.G.; Kusakabe, I.
Purification and characterization of recombinant Mortierella vinacea alpha-galactosidase I and II expressed in Saccharomyces cerevisiae
Biosci. Biotechnol. Biochem.
63
1096-1099
1999
Umbelopsis vinacea
brenda
Fujimoto, Z.; Kaneko, S.; Kim, W.D.; Park, G.G.; Momma, M.; Kobayashi, H.
The tetramer structure of the glycoside hydrolase family 27 alpha-galactosidase I from Umbelopsis vinacea
Biosci. Biotechnol. Biochem.
73
2360-2364
2009
Umbelopsis vinacea (Q02402), Umbelopsis vinacea
brenda