The enzyme specifically hydrolyses (1->4)-alpha-L-fucoside linkages in fucan. Fucans are found mainly in different species of seaweed and are sulfated polysaccharides with a backbone of (1->3)-linked or alternating (1->3)- and (1->4)-linked alpha-L-fucopyranosyl residues. In the literature, the sulfated polysaccharides are often called fucoidans. Fucoidans include polysaccharides with a relatively low proportion of fucose and some polysaccharides that have a backbone composed of other saccharides with fucose in the branching side chains. The sulfation of the alpha-L-fucopyranosyl residues may occur at positions 2 and 3. The enzyme degrades fucan to sulfated alpha-L-fucooligosaccharides but neither L-fucose nor small fucooligosaccharides are produced.
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The enzyme appears in viruses and cellular organisms
The enzyme specifically hydrolyses (1->4)-alpha-L-fucoside linkages in fucan. Fucans are found mainly in different species of seaweed and are sulfated polysaccharides with a backbone of (1->3)-linked or alternating (1->3)- and (1->4)-linked alpha-L-fucopyranosyl residues. In the literature, the sulfated polysaccharides are often called fucoidans. Fucoidans include polysaccharides with a relatively low proportion of fucose and some polysaccharides that have a backbone composed of other saccharides with fucose in the branching side chains. The sulfation of the alpha-L-fucopyranosyl residues may occur at positions 2 and 3. The enzyme degrades fucan to sulfated alpha-L-fucooligosaccharides but neither L-fucose nor small fucooligosaccharides are produced.
fucoidan from the brown alga Pelvetia canaliculata. End products include a tetrasaccharide and a hexasaccharide made of the repetition of disaccharidic units consisting of alpha-(1->3)-L-fucopyranose-2-sulfate-alpha-(1->4)-L-fucopyranose-2,3-disulfate, with the 3-linked residues at the nonreducing end
sulphated fucoidan oligosaccharides with different degree of polymerization
Lambis sp.
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the fucoidanase catalyses the hydrolysis of 1->3/1->4-alpha-L-fucans from Fucus evanescens and Fucus vesiculosus, but not 1->3-alpha-L-fucan from Saccharina cichorioides. It catalyzes the hydrolysis of 1->4-bonds in a fucoidan molecule as endo-enzyme
the enzyme catalyzes the hydrolysis of fucoidans from Fucus evanescens and Fucus vesiculosus, but not from Saccharina cichorioides. The fucoidanase does not hydrolyze carrageenan. Desulfated fucoidan from Fucus evanescens is hydrolysed very weakly in contrast to deacetylated fucoidan, which is hydrolysed more actively compared to the native fucoidan from Fucus evanescens. Analysis of the structure of the enzymatic products shows that the enzyme shows an endo-type action that is specific for (1->4)-bonds in a polysaccharide molecule built up of alternating 3- and 4-linked alpha-L-fucopyranose residues sulfated mainly at position 2
fucoidan from Korean Undaria pinnatifida sporophylls. Endo-acting fucoidanase. The strain depolymerizes fucoidan into more than 7 distinct low molecular-mass fucose-containing oligosaccharides, ranging from 305 to 3749 Da
fucoidan from Korean Undaria pinnatifida sporophylls. Endo-acting fucoidanase. The strain depolymerizes fucoidan into more than 7 distinct low molecular-mass fucose-containing oligosaccharides, ranging from 305 to 3749 Da
fucoidanase FFA2 catalyzes the cleavage of (1->4)-alpha-glycosidic bonds in the fucoidan from Fucus evanescens within a structural fragment [(->3)-alpha-L-Fucp2S-(1->4)-alpha-L-Fucp2S-(1->)]n but not in a fragment [(->3)-alpha-L-Fucp2S,4S-(1->4)-alpha-L-Fucp2S-(1->)]n. A broad number of sulfated oligosaccharides is formed after several minutes of the reaction start. Tetra- and disaccharides are formed after 15 min and 3 h of the reaction, respectively
fucoidanase FFA2 catalyzes the cleavage of (1->4)-alpha-glycosidic bonds in the fucoidan from Fucus evanescens within a structural fragment [(->3)-alpha-L-Fucp2S-(1->4)-alpha-L-Fucp2S-(1->)]n but not in a fragment [(->3)-alpha-L-Fucp2S,4S-(1->4)-alpha-L-Fucp2S-(1->)]n. A broad number of sulfated oligosaccharides is formed after several minutes of the reaction start. Tetra- and disaccharides are formed after 15 min and 3 h of the reaction, respectively
fucoidan from Fucus gardneri. The fucoidanase hydrolyzes fucoidan without desulfation to form oligosaccharides ranging from 10 to 2 fucose units plus the monosaccharide, fucose. Fucose can not be detected until late in the hydrolysis period, and sulfate release is not detectable during the first 4 hours of fucoidanase action
unsulfated or persulfated oligosaccharides are not cleaved. Tetrasaccharide and synthetic sulfated tetrasaccharide with bonds sequence (1->3)-(1->4)-(1->3) reacts much slower than the tetrasaccharide bearing bonds sequence (1->4)-(1->3)-(1->4)
unsulfated or persulfated oligosaccharides are not cleaved. Tetrasaccharide and synthetic sulfated tetrasaccharide with bonds sequence (1->3)-(1->4)-(1->3) reacts much slower than the tetrasaccharide bearing bonds sequence (1->4)-(1->3)-(1->4)
the first appearance of fucoidan enzymatic hydrolysis products in a cell-free extract is detected after 4 h of bacterial growth, and maximal fucoidanase activity is observed after 12 h of growth
the enzyme can be used for the modification of natural fucoidans to obtain more regular and easier characterized derivatives useful for research and practical applications
the enzyme can be used for the modification of natural fucoidans to obtain more regular and easier characterized derivatives useful for research and practical applications
3.2.1.212 endo-(1->4)-fucoidanase
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