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EC Tree
IUBMB Comments Wide specificity for beta-D-glucosides. Some examples also hydrolyse one or more of the following: beta-D-galactosides, alpha-L-arabinosides, beta-D-xylosides, beta-D-fucosides.
The taxonomic range for the selected organisms is: Caldicellulosiruptor saccharolyticus The enzyme appears in selected viruses and cellular organisms
Synonyms
beta-glucosidase, linamarase, beta-glu, emulsin, bglu1, zm-p60.1, bgl1b, cel3a, novozyme 188, t-cell inhibitor,
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SYNbglA
thermophilic beta-glucosidase
aryl-beta-glucosidase
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beta-1,6-glucosidase
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-
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beta-D-glucosidase
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-
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beta-D-glucoside glucohydrolase
beta-glucoside hydrolase
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p-nitrophenyl beta-glucosidase
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-
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beta-D-glucoside glucohydrolase
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beta-D-glucoside glucohydrolase
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-
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hydrolysis of O-glycosyl bond
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-
-
-
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beta-D-glucoside glucohydrolase
Wide specificity for beta-D-glucosides. Some examples also hydrolyse one or more of the following: beta-D-galactosides, alpha-L-arabinosides, beta-D-xylosides, beta-D-fucosides.
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(2-hydroxymethylphenyl)-beta-D-glucopyranoside + H2O
2-hydroxymethylphenol + beta-D-glucose
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salicin
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?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactopyranose
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-
-
-
?
2-nitrophenyl beta-D-galactopyranoside + H2O
2-nitrophenol + beta-D-galactose
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-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + beta-D-glucopyranose
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-
-
-
?
2-nitrophenyl beta-D-glucopyranoside + H2O
2-nitrophenol + D-glucopyranose
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-
-
?
2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O
beta-D-glucose + D-glucose
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sophorose
-
?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O
beta-D-glucose + D-glucose
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laminaribiose
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?
4-nitrophenyl beta-D-cellobioside + H2O
4-nitrophenol + cellobiose
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-
-
?
4-nitrophenyl beta-D-fucopyranoside + H2O
4-nitrophenol + beta-D-fucose
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-
-
?
4-nitrophenyl beta-D-fucoside + H2O
4-nitrophenol + beta-D-fucose
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-
-
?
4-nitrophenyl beta-D-galactopyranoside + H2O
4-nitrophenol + beta-D-galactose
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-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + beta-D-galactose
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-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + beta-D-glucose
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the enzyme catalysed both hydrolysis and transglycosylation
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-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
4-nitrophenyl beta-D-lactopyranoside + H2O
4-nitrophenol + beta-D-lactose
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-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
4-O-beta-D-galactopyranosyl-D-glucose + H2O
beta-D-galactose + D-glucose
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lactose
-
?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
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cellobiose
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?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
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gentiobiose
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?
cellobiose + H2O
2 beta-D-glucose
cellobiose + H2O
?
highest specific activity
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-
?
cellopentaose + H2O
?
-
-
-
?
cellotetraose + H2O
?
-
-
-
?
cellotriose + H2O
?
-
-
-
?
gentiobiose + H2O
2 beta-D-glucose
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-
-
-
?
laminaribiose + H2O
2 beta-D-glucose
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-
-
-
?
additional information
?
-
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
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-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucopyranose
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-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
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-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + beta-D-xylose
-
-
-
?
cellobiose + H2O
2 beta-D-glucose
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-
-
-
?
cellobiose + H2O
2 beta-D-glucose
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-
-
?
additional information
?
-
cello-oligosaccharides from n equal 2 to 5 as substrates using 4 mM each sugar and 3 units/mg of enzyme are completely hydrolyzed to beta-D-glucose at 70°C within 16 h
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-
?
additional information
?
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cello-oligosaccharides from n equal 2 to 5 as substrates using 4 mM each sugar and 3 units/mg of enzyme are completely hydrolyzed to beta-D-glucose at 70°C within 16 h
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-
?
additional information
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the enzyme does not hydrolyze 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-beta-D-maltopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside
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-
?
additional information
?
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the enzyme does not hydrolyze 4-nitrophenyl-alpha-D-glucopyranoside, 4-nitrophenyl-alpha-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 4-nitrophenyl-beta-D-maltopyranoside, 4-nitrophenyl-beta-D-mannopyranoside, and 4-nitrophenyl-beta-L-arabinopyranoside
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?
additional information
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the selectivity hexanol vs. water increases with increasing water activity in the reaction medium. It seems that the ideal condition for the transglycosylation reaction is a water activity close to 1.0
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-
?
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2-O-beta-D-glucopyranosyl-D-glucopyranose + H2O
beta-D-glucose + D-glucose
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sophorose
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?
3-O-beta-D-glucopyranosyl-D-glucopyranose + H2O
beta-D-glucose + D-glucose
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laminaribiose
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?
4-O-beta-D-galactopyranosyl-D-glucose + H2O
beta-D-galactose + D-glucose
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lactose
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?
4-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
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cellobiose
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?
6-O-beta-D-glucopyranosyl-D-glucose + H2O
beta-D-glucose + D-glucose
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gentiobiose
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?
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1-O-methyl-beta-D-glucose
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competitive inhibition, Ki: 17.5 mM
Co2+
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destabilizing effect
D-glucono-1,4-lactone
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Ki: 1.6 mM
D-glucono-1,5-lactone
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Ki: 0.09 mM
Hg2+
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strong inactivation at 0.5 mM, reversal by addition of 10 mM beta-mercaptoethanol
La3+
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rapid inactivation at 3 mM
N-ethylmaleimide
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rapid loss of activity at 5 mM, reversal by addition of beta-mercaptoethanol
SDS
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complete inhibition at 1 mg/ml
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2.12
2-nitrophenyl beta-D-galactopyranoside
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-
0.29
2-nitrophenyl beta-D-glucopyranoside
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-
0.44
4-nitrophenyl-beta-D-fucopyranoside
in 50 mM citrate buffer, at pH 5.5 and 70°C
0.24
4-nitrophenyl-beta-D-fucoside
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-
1.93 - 1.94
4-nitrophenyl-beta-D-galactopyranoside
0.51 - 0.67
4-nitrophenyl-beta-D-glucopyranoside
6.93
4-nitrophenyl-beta-D-xyloside
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-
1.93
4-nitrophenyl-beta-D-galactopyranoside
in 50 mM citrate buffer, at pH 5.5 and 70°C
1.94
4-nitrophenyl-beta-D-galactopyranoside
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-
0.51
4-nitrophenyl-beta-D-glucopyranoside
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-
0.67
4-nitrophenyl-beta-D-glucopyranoside
in 50 mM citrate buffer, at pH 5.5 and 70°C
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516
4-nitrophenyl-beta-D-fucopyranoside
in 50 mM citrate buffer, at pH 5.5 and 70°C
82
4-nitrophenyl-beta-D-galactopyranoside
in 50 mM citrate buffer, at pH 5.5 and 70°C
387
4-nitrophenyl-beta-D-glucopyranoside
in 50 mM citrate buffer, at pH 5.5 and 70°C
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1185
4-nitrophenyl-beta-D-fucopyranoside
in 50 mM citrate buffer, at pH 5.5 and 70°C
42
4-nitrophenyl-beta-D-galactopyranoside
in 50 mM citrate buffer, at pH 5.5 and 70°C
579
4-nitrophenyl-beta-D-glucopyranoside
in 50 mM citrate buffer, at pH 5.5 and 70°C
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0.08
crude extract, at 70°C
0.28
after 163fold purification, using 2-nitrophenyl-beta-D-galactopyranoside as substrate, at 70°C
0.3
after 163fold purification, using 2-nitrophenyl-beta-D-glucopyranoside as substrate, at 70°C
0.32
after 163fold purification, using 4-nitrophenyl-beta-D-xylopyranoside as substrate, at 70°C
0.75
after 163fold purification, using 4-nitrophenyl-beta-D-lactopyranoside as substrate, at 70°C
1.3
after 163fold purification, using 4-nitrophenyl-beta-D-cellobioside as substrate, at 70°C
10.8
after 163fold purification, using cellotetraose as substrate, at 70°C
13
after 163fold purification, using 4-nitrophenyl-beta-D-glucopyranoside as substrate, at 70°C
14.7
after 163fold purification, using 4-nitrophenyl-beta-D-fucopyranoside as substrate, at 70°C
16.2
after 163fold purification, using cellobiose as substrate, at 70°C
6.1
after 163fold purification, using 4-nitrophenyl-beta-D-galactopyranoside as substrate, at 70°C
6.8
after 163fold purification, using cellopentaose as substrate, at 70°C
9.5
after 163fold purification, using cellotriose as substrate, at 70°C
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5.5
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-
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70
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UniProt
brenda
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brenda
additional information
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brenda
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BGLS_CALSA
455
0
53492
Swiss-Prot
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54000
2 * 54000, SDS-PAGE
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homodimer
2 * 54000, SDS-PAGE
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60 - 80
the half-lives of the enzyme at 60, 70, and 80°C are 250, 24.3, and 0.4 h
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stable in the presence of cetyl-trimethylammonium bromide
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stable in the presence of urea
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tetrahydrofuran
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unstable above 20%, v/v
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heat treatment and His-Trap affinity chromatography
to homogeneity, recombinant enzyme
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expressed in Escherichia coli
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expression in Escherichia coli
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molecular biology
a reporter gene for the localization of mammalian cells and transgenic tissues based on detection of the bglA (SYNbglA) gene of Caldocellum saccharolyticum that encodes a thermophilic beta-glucosidase is presented. SYNbglA expression can be localized in situ or detected quantitatively in colorimetric assays and can be co-localized with Escherichia coli beta-galactosidase. SYNbglA can be detected in tissue wholemounts and in frozen and wax embedded sections
industry
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beta-glucosidase is a key enzyme involved in sugar-enzyme platform for fuel ethanol production from lignocellulosic biomass
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Plant, A.R.; Oliver, J.E.; Patchett, M.L.; Daniel, R.M.; Morgan, H.W.
Stability and substrate specificity of a beta-glucosidase from the thermophilic bacterium Tp8 cloned into Escherichia coli
Arch. Biochem. Biophys.
262
181-188
1988
Caldicellulosiruptor saccharolyticus
brenda
Hong, M.R.; Kim, Y.S.; Park, C.S.; Lee, J.K.; Kim, Y.S.; Oh, D.K.
Characterization of a recombinant beta-glucosidase from the thermophilic bacterium Caldicellulosiruptor saccharolyticus
J. Biosci. Bioeng.
108
36-40
2009
Caldicellulosiruptor saccharolyticus (A4XIG7), Caldicellulosiruptor saccharolyticus
brenda
McCutcheon, S.C.; Jones, K.; Cumming, S.A.; Kemp, R.; Ireland-Zecchini, H.; Saunders, J.C.; Houghton, C.A.; Howard, L.A.; Winton, D.J.
Characterization of a heat resistant beta-glucosidase as a new reporter in cells and mice
BMC Biol.
8
89
2010
Caldicellulosiruptor saccharolyticus (P10482), Caldicellulosiruptor saccharolyticus
brenda
Hansson, T.; Andersson, M.; Wehtje, E.; Adlercreutz, P.
Influence of water activity on the competition between beta-glycosidase-catalysed transglycosylation and hydrolysis in aqueous hexanol
Enzyme Microb. Technol.
29
527-534
2001
Caldicellulosiruptor saccharolyticus, Prunus dulcis, Pyrococcus furiosus
-
brenda
Singhania, R.R.; Patel, A.K.; Sukumaran, R.K.; Larroche, C.; Pandey, A.
Role and significance of beta-glucosidases in the hydrolysis of cellulose for bioethanol production
Biores. Technol.
127
500-507
2013
Aspergillus aculeatus, Aspergillus fumigatus, Aspergillus niger, Caldicellulosiruptor saccharolyticus, Thermochaetoides thermophila, Saccharomycopsis fibuligera, Humicola insolens, Penicillium citrinum, Talaromyces pinophilus, Rasamsonia emersonii, Neocallimastix patriciarum, Fomitopsis palustris, Penicillium decumbens, Trichoderma atroviride, Stachybotrys sp., Paecilomyces sp. 'thermophila', Penicillium janczewskii, Periconia sp., Schwanniomyces pseudopolymorphus, Fomitopsis sp. RCK2010, Fervidobacterium islandicum, Aspergillus fumigatus Z5
brenda