Information on EC 3.2.1.202 - endo-chitodextinase

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The expected taxonomic range for this enzyme is: Vibrio furnissii

EC NUMBER
COMMENTARY hide
3.2.1.202
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RECOMMENDED NAME
GeneOntology No.
endo-chitodextinase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of chitodextrins, releasing N,N'-diacetylchitobiose and small amounts of N,N',N''-triacetylchitotriose
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
chitin degradation II (Vibrio)
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SYSTEMATIC NAME
IUBMB Comments
(1->4)-2-acetamido-2-deoxy-beta-D-glucan diacetylchitobiohydrolase (endo-cleaving)
The enzyme, characterized from the bacterium Vibrio furnissii, is an endo-cleaving chitodextrinase that participates in the the chitin catabolic pathway found in members of the Vibrionaceae. Unlike EC 3.2.1.14, chitinase, it has no activity on chitin. The smallest substrate is a tetrasaccharide, and the final products are N,N'-diacetylchitobiose and small amounts of N,N',N''-triacetylchitotriose. cf. EC 3.2.1.200, exo-chitinase (non-reducing end), and EC 3.2.1.201, exo-chitinase (reducing end).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside + H2O
4-methylumbelliferone + N,N'-diacetylchitobiose
show the reaction diagram
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?
4-nitrophenyl beta-D-N,N'-diacetylchitobioside + H2O
4-nitrophenol + N,N'-diacetylchitobiose
show the reaction diagram
chitooligosaccharide + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose + ?
show the reaction diagram
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?
N,N',N'',N''',N'''',N'''''-hexaacetylchitohexaose + H2O
2 N,N',N''-triacetylchitotriose
show the reaction diagram
N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O
N,N'-diacetylchitobiose + N,N',N''-triacetylchitotriose
show the reaction diagram
N,N',N'',N'''-tetraacetylchitotetraose + H2O
2 N,N'-diacetylchitobiose
show the reaction diagram
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
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2 mM, 20-25% stimulation
NaCl
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activity remains relatively constant over the range 50-250 mM NaCl (pH 7.0), but decreases significantly below and above these concentrations
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside
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4-nitrophenyl beta-D-N-acetylglucosamine
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Hg2+
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5 mM, 25% loss of activity
KCl
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10-30% decrease in activity
N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose
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N,N',N'',N''',N''''-pentacetylchitopentaose
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N,N',N'',N'''-tetraacetylchitotetraose
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N,N',N''-triiacetylchitotriose
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N,N'-diacetylchitobiose
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NaCl
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activity remains relatively constant over the range 50-250 mM NaCl (pH 7.0), but decreases significantly below and above these concentrations
additional information
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not affected by 5 mM EDTA or EGTA, nor by the addition of up to 5mM Ca2+ or Zn2+
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.066
4-nitrophenyl beta-D-N,N'-diacetylchitobioside
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pH 7.4, 25°C
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.22
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside
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pH 7.5, 37°C
0.65
4-nitrophenyl beta-D-N-acetylglucosamine
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pH 7.5, 37°C
0.077
N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose
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pH 7.5, 37°C
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0.088
N,N',N'',N''',N''''-pentacetylchitopentaose
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pH 7.5, 37°C
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0.09
N,N',N'',N'''-tetraacetylchitotetraose
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pH 7.5, 37°C
0.57
N,N',N''-triiacetylchitotriose
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pH 7.5, 37°C
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1.025
N,N'-diacetylchitobiose
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pH 7.5, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
325
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substrate 4-nitrophenyl beta-D-N,N'-diacetylchitobioside, pH 7.5, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.5
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58-70% of maximum activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
35 - 37
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
proteolytic modification
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
52
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15 min, complete inactivation
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is induced by N,N'-diacetylchitobiose
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