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Information on EC 3.2.1.20 - alpha-glucosidase and Organism(s) Bacillus thermoamyloliquefaciens and UniProt Accession Q9F234

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IUBMB Comments
This single entry covers a group of enzymes whose specificity is directed mainly towards the exohydrolysis of (1->4)-alpha-glucosidic linkages, and that hydrolyse oligosaccharides rapidly, relative to polysaccharide, which are hydrolysed relatively slowly, or not at all. The intestinal enzyme also hydrolyses polysaccharides, catalysing the reactions of EC 3.2.1.3 glucan 1,4-alpha-glucosidase and, more slowly, hydrolyses (1->6)-alpha-D-glucose links.
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Bacillus thermoamyloliquefaciens
UNIPROT: Q9F234
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The taxonomic range for the selected organisms is: Bacillus thermoamyloliquefaciens
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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maltotetraose
+
3
H2O
=
4
alpha-D-glucose
+
3
=
4
Synonyms
alpha-glucosidase, maltase, neutral alpha-glucosidase, alpha-d-glucosidase, alglucosidase alfa, intestinal maltase, intestinal alpha-glucosidase, alpha-1,4-glucosidase, recombinant human gaa, alpha-glucosidase ii, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
acid maltase
-
-
-
-
AGL
-
-
-
-
alpha-1,4-glucosidase
-
-
-
-
alpha-D-glucosidase
-
-
-
-
alpha-glucopyranosidase
-
-
-
-
alpha-glucosidase
-
-
-
-
alpha-glucoside hydrolase
-
-
-
-
glucoinvertase
-
-
-
-
glucosidoinvertase
-
-
-
-
glucosidosucrase
-
-
-
-
maltase
-
-
-
-
maltase-glucoamylase
-
-
-
-
additional information
enzyme belongs to the family 31 of glycosyl hydrolases
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of D-glucose
show the reaction diagram
the enzyme has both exo-alpha-1,4-glucosidase and oligo-alpha-1,6-glucosidase activities
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
SYSTEMATIC NAME
IUBMB Comments
alpha-D-glucoside glucohydrolase
This single entry covers a group of enzymes whose specificity is directed mainly towards the exohydrolysis of (1->4)-alpha-glucosidic linkages, and that hydrolyse oligosaccharides rapidly, relative to polysaccharide, which are hydrolysed relatively slowly, or not at all. The intestinal enzyme also hydrolyses polysaccharides, catalysing the reactions of EC 3.2.1.3 glucan 1,4-alpha-glucosidase and, more slowly, hydrolyses (1->6)-alpha-D-glucose links.
CAS REGISTRY NUMBER
COMMENTARY hide
9001-42-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
isomaltose + H2O
2 D-glucose
show the reaction diagram
hydrolysis of alpha-1,6-bond
-
-
?
maltose + H2O
2 D-glucose
show the reaction diagram
hydrolysis of alpha-1,4-bond
-
-
?
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
64.7
purified alpha-glucosidase II
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
alpha-glucosidase II, isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
3 alpha-glucosidases I, II, and III
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AGL2_BACTQ
787
0
91072
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
530000
alpha-glucosidase II, native PAGE
540000
alpha-glucosidase II, gel filtration
90000
6 * 91070, alpha-glucosidase II, sequence calculation, 6 * 90000, alpha-glucosidase II, SDS-PAGE
91070
6 * 91070, alpha-glucosidase II, sequence calculation, 6 * 90000, alpha-glucosidase II, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hexamer
6 * 91070, alpha-glucosidase II, sequence calculation, 6 * 90000, alpha-glucosidase II, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
70
alpha-glucosidase II, stable up to for 10 min
80
alpha-glucosidase II, half-life 10 min
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant alpha-glucosidase II from Escherichia coli, 86fold
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of a genomic library, gene mapping, DNA and amino acid sequence determination and analysis, expression in Escherichia coli HB101 and DH5alpha
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kashiwabara, S.I.; Azuma, S.; Tsuduki, M.; Suzuki, Y.
The primary structure of the subunit in Bacillus thermoamyloliquefaciens KP1071 molecular weight 540,000 homohexameric alpha-glucosidase II belonging to the glycosyl hydrolase family 31
Biosci. Biotechnol. Biochem.
64
1379-1393
2000
Bacillus thermoamyloliquefaciens (Q9F234)
Manually annotated by BRENDA team