Information on EC 3.2.1.198 - alpha-mannan endo-1,2-alpha-mannanase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
Select one or more organisms in this record:
Show additional data
Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)


The expected taxonomic range for this enzyme is: Bacteroides

EC NUMBER
COMMENTARY hide
3.2.1.198
-
RECOMMENDED NAME
GeneOntology No.
alpha-mannan endo-1,2-alpha-mannanase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of the terminal alpha-D-mannosyl-(1->3)-alpha-D-mannose disaccharide from alpha-D-mannosyl-(1->3)-alpha-D-mannosyl-(1->2)-alpha-D-mannosyl-(1->2)-alpha-D-mannosyl side chains in fungal cell wall alpha-mannans
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
alpha-mannan 1,2-[alpha-D-mannosyl-(1->3)-alpha-D-mannose] hydrolase
The enzyme, characterized from the gut bacteria Bacteroides thetaiotaomicron and Bacteroides xylanisolvens, can also catalyse the reaction of EC 3.2.1.130, glycoprotein endo-alpha-1,2-mannosidase.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GH99 family protein
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside
show the reaction diagram
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside + H2O
4-methylumbelliferone + (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
show the reaction diagram
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside + H2O
alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside
show the reaction diagram
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranosyl-(1->2)-alpha-D-mannopyranoside + H2O
alpha-1,3-mannobiose + methyl alpha-1,2-mannobioside
show the reaction diagram
additional information
?
-
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
alpha-mannopyranosyl-1,3-isofagomine
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.038 - 1.83
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
0.404 - 2.6
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0007
4-methylumbelliferyl (alpha-D-glucopyranosyl)-(1->3)-alpha-D-mannopyranoside
Bacteroides xylanisolvens
D6D1V7
pH 7, 37C
-
0.0008 - 0.0015
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
-
3
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside
Bacteroides thetaiotaomicron
Q8A109
pH 7, 37C
-
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00062 - 0.0085
4-methylumbelliferyl (alpha-D-mannopyranosyl)-(1->3)-alpha-D-mannopyranoside
202168
0.0021 - 1.15
methyl alpha-D-mannopyranosyl-(1->3)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranosyl-(1,2)-alpha-D-mannopyranoside
214074
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in complex with inhibitor alpha-mannopyranosyl-1,3-isofagomine. The inhibitor is coordinated by residues Y46, W48, H63, W126, E156, E333 and E336
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE