Information on EC 3.2.1.195 - 20-O-multi-glycoside ginsenosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.195
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RECOMMENDED NAME
GeneOntology No.
20-O-multi-glycoside ginsenosidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a protopanaxadiol-type ginsenoside with two glycosyl residues at position 20 + H2O = a protopanaxadiol-type ginsenoside with a single glucosyl residue at position 20 + a monosaccharide
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ginsenoside degradation I
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ginsenoside degradation II
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ginsenoside metabolism
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SYSTEMATIC NAME
IUBMB Comments
protopanaxadiol-type ginsenoside 20-beta-D-glucohydrolase
The 20-O-multi-glycoside ginsenosidase catalyses the hydrolysis of the 20-O-alpha-(1->6)-glycosidic bond and the 20-O-beta-(1->6)-glycosidic bond of protopanaxadiol-type ginsenosides. The enzyme usually leaves a single glucosyl residue attached at position 20, although it can cleave the remaining glucosyl residue with a lower efficiency. Starting with a ginsenoside that is glycosylated at positions 3 and 20, such as ginsenosides Rb1, Rb2, Rb3 and Rc, the most common product is ginsenoside Rd, with a low amount of ginsenoside Rg3 also formed.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ginsenoside Rb1 + H2O
ginsenoside Rd + D-glucose
show the reaction diagram
ginsenoside Rb2 + H2O
ginsenoside Rd + L-arabinopyranose
show the reaction diagram
ginsenoside Rb3 + H2O
ginsenoside Rd + D-xylose
show the reaction diagram
ginsenoside Rc + H2O
ginsenoside Rd + L-arabinofuranose
show the reaction diagram
ginsenoside Rd + H2O
ginsenoside Rg3 + D-glucose
show the reaction diagram
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slowly hydrolyzes the 20-O-glucoside of Rd to produce very small amounts of ginsenoside Rg3
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?
additional information
?
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
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50100 mM slightly enhances the ginsenosidase type II activity
Mg2+
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50100 mM slightly enhances the ginsenosidase type II activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Hg2+
1 mM, no residual activtiy
additional information
not inhibitory at 1 mM: EDTA
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75 - 100
more than 50% of maximum activtiy
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60000
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x * 60000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 7.5
stable
738475
ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DMSO
10% v/v, 69% residual activity
Ethanol
Methanol
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis