Information on EC 3.2.1.194 - ginsenosidase type IV

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The expected taxonomic range for this enzyme is: Aspergillus

EC NUMBER
COMMENTARY hide
3.2.1.194
-
RECOMMENDED NAME
GeneOntology No.
ginsenosidase type IV
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a protopanaxatriol-type ginsenoside with a single glucosyl at position 6 + H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose
show the reaction diagram
(1b)
-
-
-
a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + 2 H2O = a protopanaxatriol-type ginsenoside with no glycosidic modification at position 6 + D-glucopyranose + a monosaccharide
show the reaction diagram
a protopanaxatriol-type ginsenoside with two glycosyl residues at position 6 + H2O = a protopanaxatriol-type ginsenoside with a single glucosyl at position 6 + a monosaccharide
show the reaction diagram
(1a)
-
-
-
SYSTEMATIC NAME
IUBMB Comments
protopanaxatriol-type ginsenoside 6-beta-D-glucohydrolase
Ginsenosidase type IV catalyses the sequential hydrolysis of the 6-O-beta-D-(1->2)-glycosidic bond or the 6-O-alpha-D-(1->2)-glycosidic bond in protopanaxatriol-type ginsenosides with a disacchride attached to the C6 position, followed by the hydrolysis of the remaining 6-O-beta-D-glycosidic bond (e.g. ginsenoside Re -> ginsenoside Rg1 -> ginsenoside F1).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ginsenoside R1 + H2O
ginsenoside Rg1 + D-xylose
show the reaction diagram
ginsenoside Re + H2O
ginsenoside Rg1 + L-rhamnose
show the reaction diagram
ginsenoside Rf + H2O
ginsenoside Rh1 + D-glucose
show the reaction diagram
ginsenoside Rg1 + H2O
ginsenoside F1 + D-glucose
show the reaction diagram
ginsenoside Rg2 + H2O
ginsenoside Rh1 + L-rhamnose
show the reaction diagram
-
the enzyme hydrolyzes 6-O-alpha-L-(1->2)-rhamnoside
-
-
?
ginsenoside Rh1 + H2O
protopanaxatriol + D-glucose
show the reaction diagram
-
the enzyme weakly hydrolyzes 6-O-beta-D-glucoside
-
-
?
additional information
?
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Mg2+
-
50-100 mM, slightly improves activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
7.06
ginsenoside R1
-
pH 6, 40C
22.2
ginsenoside Re
-
pH 6, 40C
9.43
ginsenoside Rf
-
pH 6, 40C
7.63
ginsenoside Rg1
-
pH 6, 40C
5.74
ginsenoside Rg2
-
pH 6, 40C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.68
-
pH 6.0, 40C
1.7
-
pH 6, 40C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 8
-
pH 3.0: about 70% of maximal activity, pH 8.0: about 80% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20 - 60
-
20C: about 70% of maximal activity, 60C: about 80% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
56000
-
x * 56000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE