Information on EC 3.2.1.192 - ginsenoside Rb1 beta-glucosidase

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The expected taxonomic range for this enzyme is: Paecilomyces sp.

EC NUMBER
COMMENTARY hide
3.2.1.192
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RECOMMENDED NAME
GeneOntology No.
ginsenoside Rb1 beta-glucosidase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
ginsenoside Rb1 + 2 H2O = ginsenoside Rg3 + 2 D-glucopyranose
show the reaction diagram
ginsenoside Rb1 + H2O = ginsenoside Rd + D-glucopyranose
show the reaction diagram
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ginsenoside Rd + H2O = ginsenoside Rg3 + D-glucopyranose
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
ginsenoside metabolism
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SYSTEMATIC NAME
IUBMB Comments
ginsenoside Rb1 glucohydrolase
Ginsenosidases catalyse the hydrolysis of glycosyl moieties attached to the C-3, C-6 or C-20 position of ginsenosides. They are specific with respect to the nature of the glycosidic linkage, the position and the order in which the linkages are cleaved. Ginsenoside Rb1 beta-glucosidase specifically and sequentially hydrolyses the 20-[beta-D-glucopyranosyl-(1->6)-beta-D glucopyranosyloxy] residues attached to position 20 by first hydrolysing the (1->6)-glucosidic bond to generate ginsenoside Rd as an intermediate, followed by hydrolysis of the remaining 20-O-beta-D-glucosidic bond.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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Manually annotated by BRENDA team
Paecilomyces sp. 220 Bainier
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucopyranose
show the reaction diagram
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no hydrolysis of 4-nitrophenyl-alpha-D-glucoside or 4-nitrophenyl-beta-D-xyloside
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-
?
gentiobiose + H2O
D-glucopyranose
show the reaction diagram
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-
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?
ginsenoside Rb1 + H2O
ginsenoside Rd + D-glucopyranose
show the reaction diagram
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hydrolysis of 20-O-beta-D-glucosyl-beta-D-(1->6)-glucosidic bond
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-
?
ginsenoside Rd + H2O
ginsenoside Rg3 + D-glucopyranose
show the reaction diagram
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hydrolyzes the 20-O-glucoside of Rd at 44% of the activity compared to the hydrolysis of the 20-O-beta-D-glucosyl-beta-D-(1->6)-glucosidic bond of ginsenoside Rb1
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?
additional information
?
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no hydrolysis of the 20-O-alpha-D-(1->6)-arabinopyranosidic bond of ginsenoside Rb2 and the 20-O-beta-D-(1->6)-xylosidic bond of ginsenoside Rb3. It does not hydrolyse the 3-O-beta-glucosidic bond of ginsenoside Rb1, Rb2, Rb3
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.111
4-nitrophenyl beta-D-glucoside
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pH 3.5, 45°C
0.072
ginsenoside Rb1
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pH 3.5, 45°C
0.805
ginsenoside Rd
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pH 3.5, 45°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5
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at 55°C, in 50 mM formate buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 5
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pH 2.5: about 45% of maximal activity, pH 5.0: about 45% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
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assay at
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40 - 60
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40°C: about 50% of maximal activity. 60°C: about 60% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
102000
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3 * 102000, SDS-PAGE
305000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 7
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45°C, 4 h, 10% loss of activity
678825
3.5 - 5
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45°C, 4 h, 5% loss of activity
678825
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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pH 3.5, 4 h, 10.4% loss of activity
55
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pH 3.5, 4 h, 81.5% loss of activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
only two chromatographic steps, QSepharose FF and microcrystalline cellulose column in sequence, are required to purify the enzyme to homogeneity
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