Information on EC 3.2.1.190 - dioscin glycosidase (3-O-beta-D-Glc-diosgenin-forming)

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The expected taxonomic range for this enzyme is: Sus scrofa

EC NUMBER
COMMENTARY hide
3.2.1.190
-
RECOMMENDED NAME
GeneOntology No.
dioscin glycosidase (3-O-beta-D-Glc-diosgenin-forming)
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O = 2 L-rhamnopyranose + diosgenin 3-O-beta-D-glucopyranoside
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin rhamnosidase (3-O-beta-D-Glc-diosgenin-forming)
The enzyme is involved in the hydrolysis of the steroid saponin dioscin by the digestive system of Sus scrofa (pig). cf. EC 3.2.1.189, dioscin glycosidase (diosgenin-forming).
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O
2 L-rhamnopyranose + diosgenin 3-O-beta-D-glucopyranoside
show the reaction diagram
-
the enzyme is involved in hydrolysis of the steroid saponin dioscin
-
-
?
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O
2 L-rhamnopyranose + diosgenin-3-O-beta-D-glucopyranoside
show the reaction diagram
-
-
-
-
?
additional information
?
-
-
no hydrolysis of ginsenoside Re or rutin
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-O-[alpha-L-Rha-(1->4)-[alpha-L-Rha-(1->2)]-beta-D-Glc]diosgenin + 2 H2O
2 L-rhamnopyranose + diosgenin 3-O-beta-D-glucopyranoside
show the reaction diagram
-
the enzyme is involved in hydrolysis of the steroid saponin dioscin
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ca2+
-
10-200 mM, 1.13-1.18fold activation
Mg2+
-
inhibits at 10-100 mM, slightly activates (109% of the control activity) at 200 mM
Zn2+
-
10-200 mM, 1.08-1.1fold activation
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Cu2+
-
10 mM, 94% inhibition
Fe3+
-
10 mM, 88% inhibition
Mg2+
-
inhibits at 10-100 mM, slightly activates at 200 mM
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
88
-
pH 7.2, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 9
-
pH 4.0: about 50% of maximal activity, pH 9.0: about 60% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32 - 57
-
32°C: about 85% of maximal activity, 57°C: about 35% of maximal activity
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
47000
-
x * 47000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
-
x * 47000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32 - 52
-
stable
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE