Information on EC 3.2.1.188 - avenacosidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.188
-
RECOMMENDED NAME
GeneOntology No.
avenacosidase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
avenacoside B + H2O = 26-desgluco-avenacoside B + D-glucose
show the reaction diagram
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-
-
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SYSTEMATIC NAME
IUBMB Comments
avenacoside B 26-beta-D-glucohydrolase
Isolated from oat (Avena sativa) seedlings. The product acts as a defense system against fungal infection. Also acts on avenacoside A.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-nitrophenyl-beta-D-galactoside + H2O
3-nitrophenol + D-galactose
show the reaction diagram
-
-
-
-
?
3-nitrophenyl-beta-D-glucoside + H2O
3-nitrophenol + D-glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl alpha-L-rhamnopyranoside + H2O
4-nitrophenol + L-rhamnopyranose
show the reaction diagram
4-nitrophenyl beta-D-fucoside + H2O
4-nitrophenol + D-fucose
show the reaction diagram
-
highest activity
-
-
?
4-nitrophenyl beta-D-galactoside + H2O
4-nitrophenol + D-galactose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-glucopyranoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
4-nitrophenyl beta-D-glucoside + H2O
4-nitrophenol + D-glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl beta-D-xylopyranoside + H2O
4-nitrophenol + D-xylose
show the reaction diagram
-
lowest activity
-
-
?
4-nitrophenyl-beta-galactoside + H2O
-
show the reaction diagram
-
-
-
-
?
4-nitrophenyl-beta-glucoside + H2O
-
show the reaction diagram
-
-
-
-
?
avenacoside A + H2O
26-desgluco-avenacoside A + D-glucose
show the reaction diagram
avenacoside B + H2O
26-desgluco-avenacoside B + D-glucose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
avenacoside A + H2O
26-desgluco-avenacoside A + D-glucose
show the reaction diagram
avenacoside B + H2O
26-desgluco-avenacoside B + D-glucose
show the reaction diagram
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
not influenced by 1 mM Cu2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Hg2+
-
complete inhibition at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.183
avenacoside B
-
at pH 6.5 and 25°C
IC50 VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.5
Hg2+
Avena sativa
-
at pH 6.5 and 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
1.73
-
using 4-nitrophenyl-beta-galactoside as substrate, at pH 5.4 and 34°C for 15 min
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 7
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.8 - 8
-
50% activity at pH 4.8 and 8.0, respectively
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.8 - 4.1
-
isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
bound to the tonoplast membrane
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
68000
-
x * 68000, SDS-PAGE
110000
-
x * 110000, SDS-PAGE
300000 - 350000
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pentamer or hexamer
-
5 or 6 * 60000, SDS-PAGE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0
-
dissociation by freezing/thawing leads to complete loss of enzyme activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, hydroxyapatite column chromatography, and Biogel A1.5 gel filtration
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DEAE column chromatography, and TSK 3000 gel filtration
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hydroxyapatite column chromatography
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