Information on EC 3.2.1.185 - non-reducing end beta-L-arabinofuranosidase

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The expected taxonomic range for this enzyme is: Bifidobacterium longum

EC NUMBER
COMMENTARY hide
3.2.1.185
-
RECOMMENDED NAME
GeneOntology No.
non-reducing end beta-L-arabinofuranosidase
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose + H2O = 2 beta-L-arabinofuranose
show the reaction diagram
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
beta-L-arabinofuranoside non-reducing end beta-L-arabinofuranosidase
The enzyme, which was identified in the bacterium Bifidobacterium longum JCM1217, removes the beta-L-arabinofuranose residue from the non-reducing end of multiple substrates, including beta-L-arabinofuranosyl-hydroxyproline (Ara-Hyp), Ara2-Hyp, Ara3-Hyp, and beta-L-arabinofuranosyl-(1->2)-1-O-methyl-beta-L-arabinofuranose. In the presence of 1-alkanols, the enzyme demonstrates transglycosylation activity, retaining the anomeric configuration of the arabinofuranose residue. cf. EC 3.2.1.55, non-reducing end alpha-L-arabinofuranosidase
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose + H2O
2 beta-L-arabinofuranose
show the reaction diagram
-
enzyme liberates L-arabinose from the L-arabinofuranose (Ara)-beta1,2-Ara disaccharide
-
?
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-L-hydroxyproline + H2O
2 beta-L-arabinofuranose + L-hydroxyproline
show the reaction diagram
-
-
-
?
beta-L-arabinofuranosyl-L-hydroxyproline + H2O
beta-L-arabinofuranose + L-hydroxyproline
show the reaction diagram
-
-
-
?
additional information
?
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.85
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
wild-type, pH 4.5, 37°C
0.31
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-L-hydroxyproline
wild-type, pH 4.5, 37°C
0.43
beta-L-arabinofuranosyl-L-hydroxyproline
wild-type, pH 4.5, 37°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
Bifidobacterium longum
E8MGH8
wild-type, pH 4.5, 37°C
6.3
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-L-hydroxyproline
Bifidobacterium longum
E8MGH8
wild-type, pH 4.5, 37°C
0.013
beta-L-arabinofuranosyl-L-hydroxyproline
Bifidobacterium longum
E8MGH8
wild-type, pH 4.5, 37°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.3
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranose
Bifidobacterium longum
E8MGH8
wild-type, pH 4.5, 37°C
15302
20
beta-L-arabinofuranosyl-(1->2)-beta-L-arabinofuranosyl-L-hydroxyproline
Bifidobacterium longum
E8MGH8
wild-type, pH 4.5, 37°C
42470
0.03
beta-L-arabinofuranosyl-L-hydroxyproline
Bifidobacterium longum
E8MGH8
wild-type, pH 4.5, 37°C
42471
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00003
mutant E366A, pH 4.5, 37°C
0.032
mutant E322A, pH 4.5, 37°C
0.34
mutant E338A, pH 4.5, 37°C
2.1
wild-type, pH 4.5, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacteroides thetaiotaomicron (strain ATCC 29148 / DSM 2079 / NCTC 10582 / E50 / VPI-5482)
Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
Bifidobacterium longum subsp. longum (strain ATCC 15707 / DSM 20219 / JCM 1217 / NCTC 11818 / E194b)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
74000
x * 74000, SDS-PAGE, x * 74319, calculated
74319
x * 74000, SDS-PAGE, x * 74319, calculated
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 74000, SDS-PAGE, x * 74319, calculated
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E322A
1.5% of wild-type activity
E338A
16% of wild-type activity
E366A
0.0013% of wild-type activity