Information on EC 3.2.1.173 - rhamnogalacturonan galacturonohydrolase

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The expected taxonomic range for this enzyme is: Aspergillus aculeatus

EC NUMBER
COMMENTARY hide
3.2.1.173
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RECOMMENDED NAME
GeneOntology No.
rhamnogalacturonan galacturonohydrolase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Exohydrolysis of the alpha-D-GalA-(1->2)-alpha-L-Rha bond in rhamnogalacturonan oligosaccharides with initial inversion of configuration releasing D-galacturonic acid from the non-reducing end of rhamnogalacturonan oligosaccharides.
show the reaction diagram
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-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
rhamnogalacturonan type I degradation I (fungi)
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SYSTEMATIC NAME
IUBMB Comments
rhamnogalacturonan oligosaccharide alpha-D-GalA-(1->2)-alpha-L-Rha galacturonohydrolase
The enzyme is part of the degradation system for rhamnogalacturonan I in Aspergillus aculeatus.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
no activity in Beta vulgaris
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha + H2O
beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha
show the reaction diagram
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-
-
-
?
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha + H2O
beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha
show the reaction diagram
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-
-
-
?
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]4-(1->2)-alpha-L-Rha + H2O
beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]4-(1->2)-alpha-L-Rha
show the reaction diagram
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-
-
-
?
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha + H2O
beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha
show the reaction diagram
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-
-
-
?
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]6-(1->2)-alpha-L-Rha + H2O
beta-D-GalA + [alpha-L-Rha-(1->4)-alpha-D-GalA-]6-(1->2)-alpha-L-Rha
show the reaction diagram
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-
-
-
?
rhamnogalacturonan I oligosaccharides with alpha-D-GalA at the non-reducing end + H2O
beta-D-galacturonic acid + shortened rhamnogalacturonan I oligosaccharides with beta-D-galacturonic acid at the reducing end and at the nonreducing en
show the reaction diagram
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active toward all rhamnogalacturonan types of substrate, except for those with a 4,5-deoxy-4,5-unsaturated D-galactopyranosyluronic acid at the nonreducing end
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?
additional information
?
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no activity with: CM-cellulose, crystalline cellulose, xylan from oat spelts, soluble starch, potato arabino-beta-(1,4)-galactan, larchwood arabino-beta-(1,3)/(1,6)-galactan (stractan), linear arabinan, polygalacturonic acid with or without 1 mM CaCl2 added, 4-nitrophenyl-alpha-L-Araf, 4-nitrophenyl-alpha-D-Galp, 4-nitrophenyl-beta-D-Galp, 4-nitrophenyl-alpha-D-Xylp, 4-nitrophenyl-beta-D-Xylp, 4-nitrophenyl-alpha-D-Manp, 4-nitrophenylb-D-Manp, 4-nitrophenyl-alpha-L-Fucp, 4-nitrophenyl-beta-D-Fucp, 4-nitrophenyl-alpha-D-Glcp, 4-nitrophenyl-beta-D-Glcp, 4-nitrophenyl-alpha-L-Rhap, 4-nitrophenyl-beta-D-GlcpA, and 4-nitrophenyl-beta-D-GalpA
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.21
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha
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40°C, pH 5.0
0.075
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha
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40°C, pH 5.0
0.085
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha
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40°C, pH 5.0
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.4
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha
Aspergillus aculeatus
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40°C, pH 5.0
1.5
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha
Aspergillus aculeatus
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40°C, pH 5.0
1.8
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha
Aspergillus aculeatus
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40°C, pH 5.0
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
11
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]2-(1->2)-alpha-L-Rha
Aspergillus aculeatus
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40°C, pH 5.0
40495
20
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]3-(1->2)-alpha-L-Rha
Aspergillus aculeatus
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40°C, pH 5.0
40496
21
alpha-D-GalA-[(1->2)-alpha-L-Rha-(1->4)-alpha-D-GalA-]5-(1->2)-alpha-L-Rha
Aspergillus aculeatus
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40°C, pH 5.0
40497
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4
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McIlvaine buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.5 - 4.5
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pH 3.5: about 75% of maximal activity, pH 4.5: about 75% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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NaOAc buffer, pH 5.0
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.1
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in isoelectric focusing a major band at pI 5.12 and minor bands at 5.0, 5.07, and 5.2 are found. Preparative isoelectric focusing shows maximal activity of RG-galacturonohydrolase in the collected fractions with a pH of 4.9 and 5.0
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
66000
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x * 66000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
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x * 66000, SDS-PAGE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.5 - 7
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most stable for 1 h at 40°C
710299
3 - 6
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most stable for 24 h at 40°C
710299
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
60
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stable for at least 3 h
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE