Information on EC 3.2.1.159 - alpha-neoagaro-oligosaccharide hydrolase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.159
-
RECOMMENDED NAME
GeneOntology No.
alpha-neoagaro-oligosaccharide hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of the (1->3)-alpha-L-galactosidic linkages of neoagaro-oligosaccharides that are smaller than a hexamer, yielding 3,6-anhydro-L-galactose and D-galactose
show the reaction diagram
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
agarose degradation
-
-
porphyran degradation
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-
SYSTEMATIC NAME
IUBMB Comments
alpha-neoagaro-oligosaccharide 3-glycohydrolase
When neoagarohexaose is used as a substrate, the oligosaccharide is cleaved at the non-reducing end to produce 3,6-anhydro-L-galactose and agaropentaose, which is further hydrolysed to agarobiose and agarotriose. With neoagarotetraose as substrate, the products are predominantly agarotriose and 3,6-anhydro-L-galactose. In Vibrio sp. the actions of EC 3.2.1.81, beta-agarase and EC 3.2.1.159 can be used to degrade agarose to 3,6-anhydro-L-galactose and D-galactose.
CAS REGISTRY NUMBER
COMMENTARY hide
60063-77-6
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain MK03
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Manually annotated by BRENDA team
strain JT0107
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Manually annotated by BRENDA team
strain JT0107
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-1,3-neoagarobiose + H2O
3,6-anhydro-L-galactose + D-galactose
show the reaction diagram
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40% hydrolysis within 30 min
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-
?
alpha-1,3-neoagarooligosaccharide + H2O
agaropentaose + agarotriose + agarobiose + D-galactose
show the reaction diagram
neoagarobiose + H2O
?
show the reaction diagram
neoagarohexaose + H2O
3,6-anhydro-L-galactose + agaropentaose
show the reaction diagram
-
complete hydrolysis within 30 min
-
-
?
neoagarotetraose + H2O
3,6-anhydro-L-galactose + agarotriose
show the reaction diagram
-
complete hydrolysis within 30 min
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-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MgCl2
-
2 mM, 163% of initial activity
SnCl2
-
2 mM, 121% of initial activity
ZnCl2
-
2 mM, 128% of initial activity
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
AgNO3
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2 mM, 9% residual activity
CuCl2
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2 mM, 7% residual activity
EDTA
-
2 mM, 47% residual activity
HgCl2
-
2 mM, no residual activity
NiCl2
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2 mM, 10% residual activity
p-chloromercuribenzoate
-
0.2 mM 60% residual activity
SDS
-
2 mM, 53% residual activity
SrCl2
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2 mM, 43% residual activity
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
5.37
neoagarobiose
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pH 7.7, 30°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
22.2
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pH 7.0, 30°C
92
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pH 7.7, 30°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7.7
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-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.2 - 8.9
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drastical reduction in activity below or above
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.6
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isoelectric focusing
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Zobellia galactanivorans (strain DSM 12802 / CCUG 47099 / CIP 106680 / NCIMB 13871 / Dsij)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
84000
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gel filtration
320000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
octamer
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8 * 42000, SDS-PAGE
additional information
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.8 - 8.6
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stable within this range
665765
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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rapid loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, phosphate buffer pH 7.8, 30% glycerol, 2 months, 60% residual activity
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4°C, phosphate buffer pH 7.8, 3 weeks, 60% residual activity, 2 months, 30% residual activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE