Information on EC 3.2.1.155 - xyloglucan-specific exo-beta-1,4-glucanase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.155
-
RECOMMENDED NAME
GeneOntology No.
xyloglucan-specific exo-beta-1,4-glucanase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->4)-D-glucosidic linkages in xyloglucans so as to successively remove oligosaccharides from the chain end.
show the reaction diagram
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
xyloglucan degradation II (exoglucanase)
-
-
SYSTEMATIC NAME
IUBMB Comments
[(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan exo-glucohydrolase
The enzyme removes XXXG heptasaccharides, XXLG/XLXG octasaccharides and XLLG nonasaccharides from the end of tamarind seed xyloglucan polymers in a processive manner. Hydrolysis occurs at the unsubstituted D-glucopyranose residue in the main backbone. It is not known whether the cleavage takes place at the reducing or non-reducing end of the polymer. Very low activity with beta-D-glucans. The enzyme from Chrysosporium lucknowense shifts to an endoglucanase mode when acting on linear substrates without bulky substituents on the polymeric backbone such as barley beta-glucan.
CAS REGISTRY NUMBER
COMMENTARY hide
1000598-79-7
-
76901-10-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain KM21
SwissProt
Manually annotated by BRENDA team
strain KM21
SwissProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl xyloglucan derivatives + H2O
oligosaccharides
show the reaction diagram
artificial substrate
-
-
?
beta-glucan + H2O
beta-glucan oligosaccharides
show the reaction diagram
carboxymethyl cellulose + H2O
carboxymethyl cellulose oligosaccharides
show the reaction diagram
tamarind xyloglucan + H2O
oligosaccharides
show the reaction diagram
highly specific for, exo-type reaction
with end products XXXG, XXLG/XLXG, and XLLG
-
?
tamarind xyloglucan + H2O
oligosacchrides
show the reaction diagram
XXXGXXXG + H2O
XXX + XXXG + GXXXG
show the reaction diagram
-
-
-
?
xyloglucan + H2O
?
show the reaction diagram
Xgh74 contributes to the in vivo degradation of the hemicellulose xyloglucan and xylan by the cellulosome of Clostridium thermocellum
-
-
?
xyloglucan + H2O
cellotetraose
show the reaction diagram
tamarind xyloglucan. Xgh74A hydrolyzes every fourth beta-1,4-glucan bond in the xyloglucan backbone, thus producing decorated cellotetraose units
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
xyloglucan + H2O
?
show the reaction diagram
Q70DK5
Xgh74 contributes to the in vivo degradation of the hemicellulose xyloglucan and xylan by the cellulosome of Clostridium thermocellum
-
-
?
xyloglucan + H2O
xyloglucan oligosaccharides
show the reaction diagram
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
12 - 30
beta-Glucan
46
carboxymethylcellulose
Trichoderma reesei
Q7Z9M8
-
-
3 - 99
xyloglucan
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.4
hydrolysis of tamarid xyloglucan; hydrolysis of tamarind seed xyloglucan
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.3 - 6.9
more than 50% of maximal activity in the pH-range 4.5-7.2
4.5 - 7.2
-
more than 50% of maximal activity in the pH-range 4.5-7.2
5.7 - 7.8
50% of maximal activity at pH 5.7 and at pH 7.8; more than 50% of maximal activity in the pH-range 5.7-7.8, hydrolysis of tamarind seed xyloglucan
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75
; hydrolysis of tamarind seed xyloglucan
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.8
-
isoelectric focusing
3.8
-
isoelectric focusing; isoelectrofocusing
4.1 - 4.3
isoelectric focusing, several enzyme forms; isoelectrofocusing, there may be different forms of the enzyme resulting from O-glycosylation
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
32000
-
x * 32000, SDS-PAGE
78000
-
x * 78000, SDS-PAGE
105000
x * 105000, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
peptide fingerprinting
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
2 putative N-glycosylation sites in Cel74A; enzyme contains two putative N-glycosylation sites. There may be different forms of the enzyme resulting from O-glycosylation
additional information
Cel74A contains a signal peptide
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5
-
40°C, stable for at least 24 h
658199
5 - 7.5
stable
667262
5
40°C, stable for at least 24 h
658199
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
stable up to
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
by ion exchange chromatography, to homogeneity
-
further purification of the commercial preparation, by ion exchange chromatography, to homogeneity
recombinant
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis