Information on EC 3.2.1.137 - mannan exo-1,2-1,6-alpha-mannosidase

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The expected taxonomic range for this enzyme is: Cellulomonas sp.

EC NUMBER
COMMENTARY hide
3.2.1.137
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RECOMMENDED NAME
GeneOntology No.
mannan exo-1,2-1,6-alpha-mannosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->2)-alpha-D- and (1->6)-alpha-D- linkages in yeast mannan, releasing D-mannose
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Fructose and mannose metabolism
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SYSTEMATIC NAME
IUBMB Comments
(1->2)-(1->6)-alpha-D-mannan D-mannohydrolase
Mannose residues linked alpha-D-1,3- are also released, but very slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
123175-72-4
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
production of the enzyme strongly induced by yeast extract, 2 different MW forms
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
D-mannan
D-mannose
show the reaction diagram
-
hydrolysis of 1,2-alpha-D-, and 1,6-alpha-D-linkages in yeast mannan, releasing D-mannose, hydrolysis rate of alpha-1,3-linkage is very low
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?
D-mannan + H2O
D-mannose
show the reaction diagram
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hydrolysis of 1,2-alpha-D-, and 1,6-alpha-D-linkages in yeast mannan, releasing D-mannose
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-
?
additional information
?
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only slight activity towards 4-nitrophenyl-alpha-D-mannoside and no hydrolysis of O-alpha-D-mannopyranosyl-1,2-D-mannitol
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
D-mannan + H2O
D-mannose
show the reaction diagram
-
hydrolysis of 1,2-alpha-D-, and 1,6-alpha-D-linkages in yeast mannan, releasing D-mannose
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4-chloromercuribenzoate
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97% inhibition at 1 mM
Cu2+
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88% inhibition at 1 mM
EDTA
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complete inhibition at 1 mM
Hg2+
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98% inhibition at 1 mM
Zn2+
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68% inhibition at 1 mM
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.5 - 200
D-mannan
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9
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pH 5: about 20% of activity maximum, pH 9: about 15% of activity maximum
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.2
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pH range 3.5-10 in a 0-60% sucrose gradient
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
450000
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gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 9.5
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4°C, 48 h, stable
646830
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
-
10 min, stable
50
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10 min, 50% loss of activity
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
high molecular weight form
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