Information on EC 3.2.1.134 - difructose-anhydride synthase

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The expected taxonomic range for this enzyme is: Aspergillus fumigatus

EC NUMBER
COMMENTARY hide
3.2.1.134
-
RECOMMENDED NAME
GeneOntology No.
difructose-anhydride synthase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
bis-D-fructose 2',1:2,1'-dianhydride + H2O = inulobiose
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
bis-D-fructose 2',1:2,1'-dianhydride fructohydrolase
Produces difructose anhydride by the reverse reaction of partial hydrolysis, forming an alpha-fructosidic linkage.
CAS REGISTRY NUMBER
COMMENTARY hide
121479-55-8
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
inulobiose
bis-D-fructose 2',1:2,1' dianhydride + H2O
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
inulobiose
bis-D-fructose 2',1:2,1' dianhydride + H2O
show the reaction diagram
-
might act as a regulator for a piecemeal use of inulin as a carbon source
-
-
r
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
L-cysteine
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
241
bis-D-fructose 2',1:2,1' dianhydride
-
pH 6, 37°C
377
Inulobiose
-
pH 6, 37°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.5
-
pH gradient 3.5-10
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8.6
-
stable at 37°C
646807
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
-
stable up to 50°C at pH 6
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-24°C, stable for several months
-
under toluene in the refrigerator at pH 6.0, stable for several months
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE