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chitosan + H2O
?
a chitosanase classification system is suggested which is based on specificities and preferences of subsites (-2) to (+2)
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?
chitosan + H2O
GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcN + GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN
chitosan with 85% degree of deacetylation. Inserting two surface loops, the endo-type chitosanase Csn is converted into an exo-type chitosanase. The chimeric chitosanase has 3% of wild-type activity and GlcNbeta(1-4)GlcN is the dominant product, whereas a mixture of GlcNbeta(1-4)GlcN, GlcNbeta(1-4)GlcNbeta(1-4)GlcN and GlcNbeta(1-4)GlcNbeta(1-4)GlcNbeta(1-4)GlcN is obtained with the wild-type endo-chitosanase. Chimeric Csn catalyzes the hydrolysis of chitosan with a smaller rate of viscosity decrease than the wild-type
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-
?
chitotriose + H2O
?
-
-
-
?
acetylated chitosan + H2O
?
-
-
-
-
?
carboxymethylchitosan + H2O
?
-
-
-
-
?
chitosan + H2O
?
-
-
-
-
?
chitosan + H2O
additional information
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chitosan + H2O
additional information
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-
-
-
?
chitosan + H2O
additional information
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-
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-
?
chitosan + H2O
additional information
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-
the smallest of the substrates is a tetramer of glucosamine
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
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-
substrates with less than 40% deacetylation are not affected by the enzyme
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
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-
most susceptible substrate is: 80% deacetylated chitosan
mixture of dimer and trimer of glucosamine
?
chitosan + H2O
additional information
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-
hydrolysis of: glycol chitosan
mixture of dimer and trimer of glucosamine
?
additional information
?
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-
no activity with chitin (i.e. 100% acetylated chitosan)
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-
?
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Yabuki, M.; Uchiyama, A.; Suzuki, K.; Ando, A.; Fujii, T.
Purification and properties of chitosanase from Bacillus circulans MH-K1
J. Gen. Appl. Microbiol.
34
255-270
1988
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Bacillus circulans MH-K1 chitosanase: amino acid residues responsible for substrate binding
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138
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2005
Niallia circulans (P33673), Niallia circulans MH-K1 (P33673)
brenda
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21
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Antonie van Leeuwenhoek
104
737-748
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brenda
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8
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Bacillus sp. No.7-M, Streptomyces sp. N174 (P33665), Niallia circulans (P33673), Niallia circulans MH-K1 (P33673)
brenda