Information on EC 3.2.1.128 - glycyrrhizin hydrolase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY
3.2.1.128
-
RECOMMENDED NAME
GeneOntology No.
glycyrrhizin hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
glycyrrhizin + H2O = beta-D-glucuronosyl-(1->2)-D-glucuronate + glycyrrhetinate
show the reaction diagram
pathway
-
glycyrrhizin + H2O = beta-D-glucuronosyl-(1->2)-D-glucuronate + glycyrrhetinate
show the reaction diagram
the glycyrrhizin-hydrolysing enzyme is separated from the general beta-glucorinidase EC 3.2.1.31
-
glycyrrhizin + H2O = beta-D-glucuronosyl-(1->2)-D-glucuronate + glycyrrhetinate
show the reaction diagram
the glycyrrhizin-hydrolysing enzyme is separated from the general beta-glucorinidase EC 3.2.1.31
-
glycyrrhizin + H2O = beta-D-glucuronosyl-(1->2)-D-glucuronate + glycyrrhetinate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
glycyrrhizinate glucuronosylhydrolase
The enzyme from Aspergillus niger is specific for the hydrolysis of the triterpenoid glycoside glycyrrhizin from roots of Glycyrrhiza sp.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
GL beta-D-glucoronidase
-
-
-
-
GL beta-D-glucoronidase I
-
-
-
-
GL hydrolase
-
-
glycyrrhizin beta-D-glucoronidase
-
-
-
-
glycyrrhizin beta-D-glucoronidase I
-
-
-
-
glycyrrhizin hydrolase
-
-
-
-
glycyrrhizin hydrolase
-
-
glycyrrhizinic acid hydrolase
-
-
-
-
hydrolase, glycyrrhizin beta-
-
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
102484-56-0
-
108891-59-4
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
Aspergillus niger GRM 3
GRM 3
-
-
Manually annotated by BRENDA team
human feces
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
chenodeoxycholic acid 3-O-beta-D-glucuronide + H2O
chenodeoxycholic acid + glucuronic acid
show the reaction diagram
-
-
-
?
chikusetsusaponin-IVa + H2O
beta-D-glucopyranosyl oleanolate + D-glucuronic acid
show the reaction diagram
-
from Panax japonicus
-
?
chikusetsusaponin-V + H2O
beta-D-glucopyranosyl oleanolate + 2-O-beta-D-glucopyranosyl D-glucuronic acid
show the reaction diagram
-
i.e. ginsenoside-Ro, from Panax japonicus
-
?
glycyrrhizinic acid + H2O
glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
show the reaction diagram
-
-
-
?
glycyrrhizinic acid + H2O
glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
show the reaction diagram
-
-
-
?
glycyrrhizinic acid + H2O
glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
show the reaction diagram
-
-
-
?
glycyrrhizinic acid + H2O
glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
show the reaction diagram
-
substrate specificity, selectively hydrolyzes the 3-O-beta-D-glucuronide linkage of glycyrrhinic acid
-
?
glycyrrhizinic acid + H2O
glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
show the reaction diagram
-
a pentacyclic triterpene saponin from liquorice roots, best substrate
-
?
glycyrrhizinic acid + H2O
glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
show the reaction diagram
-
enzyme is involved in glycyrrhizin pathway
-
?
glycyrrhizinic acid + H2O
glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
show the reaction diagram
Aspergillus niger GRM 3
-
a pentacyclic triterpene saponin from liquorice roots, best substrate
-
?
glycyrrhizinic acid + H2O
glycyrrhetic acid monoglucuronide + beta-D-glucose
show the reaction diagram
-
-
-
-
?
hemsloside Ma1 + H2O
beta-D-glucopyranosyl oleanolate + 3-O-alpha-L-arabinopyranosyl D-glucuronic acid
show the reaction diagram
-
from Hemsleya macrosperma C.Y.Wu
-
?
pseudo-ginsenoside-RT1 + H2O
beta-D-glucopyranosyl oleanolate + 2-O-beta-D-xylopyranosyl D-glucuronic acid
show the reaction diagram
-
from Panax pseudoginseng ssp. himalaicus Hara
-
?
hemsloside Ma2 + H2O
beta-D-glucopyranosyl oleanolate + 2-O-beta-D-glucopyranosyl-(3-O-alpha-L-arabinopyranosyl) alpha-D-glucuronic acid
show the reaction diagram
-
from Hemsleya macrosperma C.Y.Wu
-
?
additional information
?
-
-
no substrates are 4-nitrophenyl beta-D-glucuronide, phenolphthalein beta-D-glucuronide
-
-
-
additional information
?
-
-
chikusetsusaponin-IV, stipuleanosides R1 and 2, from Panax stipuleanatus H.T.Tsai and K.M.Feng, chikusetsusaponin-IVa ethyl or chikusetsusaponin-V methyl ester and beta-D-glucuronides of small-size aliphatic alcohols e.g. methanol, propanol or cyclohexanol, 5-alpha-cholestan-3beta-ol, l-menthol or d-menthol
-
-
-
additional information
?
-
Aspergillus niger GRM 3
-
no substrates are 4-nitrophenyl beta-D-glucuronide, phenolphthalein beta-D-glucuronide
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycyrrhizinic acid + H2O
glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
show the reaction diagram
-
enzyme is involved in glycyrrhizin pathway
-
?
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
Ba2+
-
slight stimulatory effect
Mn2+
-
slight stimulatory effect
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
Ca2+
-
slight inhibitory effect
Cu2+
-
strong inhibitory effect
Fe2+
-
slight inhibitory effect
-
glycyrrhizinic acid
-
inhibits the crude enzyme activity when concentration is up to 60 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
EDTA
-
slight stimulatory effect
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
10.98
-
glycyrrhizinic acid
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3
4.8
-
about half-maximal activity at pH 3 and 4.8
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
45
-
-
at pH 5
55
-
-
enzyme activity gradually declines at temperatures beyond 55C
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
30
53
-
about 63% of maximal activity at 30C and about half-maximal activity at 53C, pH 5
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
150000
-
-
gel filtration
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5
6.5
-
the enzyme is stable at 40C
6
-
-
stable only at pH 6. The enzyme loses its activity entirely when pH is beyond 8.0. It is more prone to inactivity at high pH conditions than at low pH conditions
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
55
-
-
stable at temperatures below 55C. When exposed to 70C, it loses 90% of its activity
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE