Information on EC 3.2.1.128 - glycyrrhizin hydrolase

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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.128
-
RECOMMENDED NAME
GeneOntology No.
glycyrrhizin hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
glycyrrhizin + H2O = beta-D-glucuronosyl-(1->2)-D-glucuronate + glycyrrhetinate
show the reaction diagram
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
glycyrrhizinate glucuronosylhydrolase
The enzyme from Aspergillus niger is specific for the hydrolysis of the triterpenoid glycoside glycyrrhizin from roots of Glycyrrhiza sp.
CAS REGISTRY NUMBER
COMMENTARY hide
102484-56-0
-
108891-59-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
GRM 3
-
-
Manually annotated by BRENDA team
-
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
chenodeoxycholic acid 3-O-beta-D-glucuronide + H2O
chenodeoxycholic acid + glucuronic acid
show the reaction diagram
-
-
-
?
chikusetsusaponin-IVa + H2O
beta-D-glucopyranosyl oleanolate + D-glucuronic acid
show the reaction diagram
-
from Panax japonicus
-
?
chikusetsusaponin-V + H2O
beta-D-glucopyranosyl oleanolate + 2-O-beta-D-glucopyranosyl D-glucuronic acid
show the reaction diagram
-
i.e. ginsenoside-Ro, from Panax japonicus
-
?
glycyrrhizinic acid + H2O
glycyrrhetic acid monoglucuronide + beta-D-glucose
show the reaction diagram
-
-
-
-
?
glycyrrhizinic acid + H2O
glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
show the reaction diagram
hemsloside Ma1 + H2O
beta-D-glucopyranosyl oleanolate + 3-O-alpha-L-arabinopyranosyl D-glucuronic acid
show the reaction diagram
-
from Hemsleya macrosperma C.Y.Wu
-
?
hemsloside Ma2 + H2O
beta-D-glucopyranosyl oleanolate + 2-O-beta-D-glucopyranosyl-(3-O-alpha-L-arabinopyranosyl) alpha-D-glucuronic acid
show the reaction diagram
-
from Hemsleya macrosperma C.Y.Wu
-
?
pseudo-ginsenoside-RT1 + H2O
beta-D-glucopyranosyl oleanolate + 2-O-beta-D-xylopyranosyl D-glucuronic acid
show the reaction diagram
-
from Panax pseudoginseng ssp. himalaicus Hara
-
?
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glycyrrhizinic acid + H2O
glycyrrhetinic acid + D-glucuronyl beta-1,2-D-glucuronic acid
show the reaction diagram
-
enzyme is involved in glycyrrhizin pathway
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Ba2+
-
slight stimulatory effect
Mn2+
-
slight stimulatory effect
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Ca2+
-
slight inhibitory effect
Cu2+
-
strong inhibitory effect
Fe2+
-
slight inhibitory effect
glycyrrhizinic acid
-
inhibits the crude enzyme activity when concentration is up to 60 mM
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
EDTA
-
slight stimulatory effect
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
10.98
glycyrrhizinic acid
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.1 - 4.5
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 4.8
-
about half-maximal activity at pH 3 and 4.8
5.8 - 6.2
-
-
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
45
-
at pH 5
55
-
enzyme activity gradually declines at temperatures beyond 55C
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
30 - 53
-
about 63% of maximal activity at 30C and about half-maximal activity at 53C, pH 5
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
the highest activity
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
150000
-
gel filtration
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 6.5
-
the enzyme is stable at 40C
646708
6
-
stable only at pH 6. The enzyme loses its activity entirely when pH is beyond 8.0. It is more prone to inactivity at high pH conditions than at low pH conditions
681484
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55
-
stable at temperatures below 55C. When exposed to 70C, it loses 90% of its activity
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE