Information on EC 3.2.1.127 - 1,6-alpha-L-fucosidase

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The expected taxonomic range for this enzyme is: Eukaryota, Archaea

EC NUMBER
COMMENTARY hide
3.2.1.127
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RECOMMENDED NAME
GeneOntology No.
1,6-alpha-L-fucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Hydrolysis of (1->6)-linkages between alpha-L-fucose and N-acetyl-D-glucosamine in glycopeptides such as immunoglobulin G glycopeptide and fucosyl-asialo-agalacto-fetuin
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis
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lysosomal hydrolase, identified in tissues and serum, serum FUCA activity correlated with early detection in hepatocellular carcinoma
hydrolysis of O-glycosyl bond
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SYSTEMATIC NAME
IUBMB Comments
6-L-fucosyl-N-acetyl-D-glucosaminylglycopeptide fucohydrolase
The enzyme from Aspergillus niger does not act on 1,2-, 1,3-, or 1,4-L-fucosyl linkages.
CAS REGISTRY NUMBER
COMMENTARY hide
102925-35-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
-
-
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
show the reaction diagram
alpha-1,6-fucosyl asialo-agalacto-fetuin + H2O
alpha-L-fucose + asialo-agalacto-fetuin
show the reaction diagram
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-
-
-
?
immunglobulin G glycopeptide + H2O
?
show the reaction diagram
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good substrate
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-
?
immunoglobulin G glycopeptide + H2O
fragments of immunoglobuline G glycopeptide
show the reaction diagram
additional information
?
-
-
alpha-1,2-, alpha-1,3- or alpha-1,4-alpha-L-fucosides are not cleaved, alpha-L-fucosyl asialo-fetuin is no substrate
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
4-nitrophenyl-alpha-L-fucopyranoside + H2O
4-nitrophenol + L-fucose
show the reaction diagram
-
-
-
-
?
immunoglobulin G glycopeptide + H2O
fragments of immunoglobuline G glycopeptide
show the reaction diagram
-
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00000277
alpha-L-fucosyl asialo-agalacto-fetuin
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.000000606
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additional information
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5.5 micromol/min/l, progressive disease group; 5.9 micromol/min/l, nonresponders to trastuzumab; 6.1 micromol/min/l, spectrophotometer citrate buffer pH 4.5, 3 h incubation at 37 degree Celsius, optical density at 405 nm, average over all patient; 6.3 micromol/min/l, non-progressive disease group; 7.2 micromol/min/l, responders to trastuzumab
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
37
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assay at
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
-
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Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
57000
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9 * 57000, SDS-PAGE, crystal structure
57390
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monomer, calculated from amino acid sequence
180000
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gel filtration
508000
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gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
nonamer
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9 * 57000, SDS-PAGE, crystal structure
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial, 156fold
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli BL21(DE3)
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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progression-free survival significantly longer in high FUCA activity group than in low FUCA activity group, not correlated to clinical response to trastuzumab but maybe useful as biomarker for predicting progression-free survival for trastuzumab treatment