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Information on EC 3.2.1.123 - endoglycosylceramidase and Organism(s) Cyanea nozakii and UniProt Accession Q9GV16

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EC Tree
IUBMB Comments
An enzyme from Rhodococcus sp. that degrades various acidic and neutral glycosphingolipids to oligosaccharides and ceramides, by cleaving a glucosyl bond. Does not act on monoglycosylceramides. cf. EC 3.2.1.62 glycosylceramidase.
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This record set is specific for:
Cyanea nozakii
UNIPROT: Q9GV16
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Word Map
The taxonomic range for the selected organisms is: Cyanea nozakii
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
endoglycoceramidase, ceramide glycanase, egcase, egcase ii, egalc, egcase i, endoglycoceramidase i, endogalactosylceramidase, endoglycosylceramidase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
endoglycoceramidase
-
ceramidase, endoglycosyl-
-
-
-
-
ceramide glycanase
-
-
-
-
EGCase
-
-
-
-
endoglycoceramidase
-
-
-
-
glycosyl-N-acetyl-sphingosine 1,1-beta-D-glucanohydrolase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
oligoglycosylglucosyl-(1<->1)-ceramide glycohydrolase
An enzyme from Rhodococcus sp. that degrades various acidic and neutral glycosphingolipids to oligosaccharides and ceramides, by cleaving a glucosyl bond. Does not act on monoglycosylceramides. cf. EC 3.2.1.62 glycosylceramidase.
CAS REGISTRY NUMBER
COMMENTARY hide
105503-61-5
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide + H2O
ceramide + Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glc
show the reaction diagram
37°C, pH 3.0, 0.2% (w/v) Triton X-100
-
-
?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
show the reaction diagram
i.e. GM1
-
-
?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
show the reaction diagram
GD1b
-
-
?
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
Galbeta(1-3)GalNAcbeta(1-4)Galbeta(1-4)Glc + ceramide
show the reaction diagram
Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
Galbeta(1-4)Glc + ceramide
show the reaction diagram
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
show the reaction diagram
GT1b
-
-
?
NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
show the reaction diagram
i.e. GM3
-
-
?
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-8)NeuAcalpha(2-3))Galbeta(1-4)Glc + ceramide
show the reaction diagram
GQ1b
-
-
?
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
NeuAcalpha(2-8)NeuAcalpha(2-3)Galbeta(1-4)Glc + ceramide
show the reaction diagram
GD3
-
-
?
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide + methanol
?
show the reaction diagram
-
i.e. GM1
products may be attributed to GM-1 oligosaccharide II3NeuAcGgOse4 and methyl-GM-1 oligosaccharide methyl-II3NeuAcGgOse4
?
Galbeta(1-4)Glcbeta(1-1)ceramide + H2O
Galbeta(1-4)Glc + ceramide
show the reaction diagram
-
i.e. lactosylceramide
i.e. lactose
r
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
Hg2+
complete inhibition at 2 mM
SDS
complete inhibition at 0.1%
taurodeoxycholate
strong inhibitor
ethanol
-
excess amounts inhibit
methanol
-
excess amounts inhibit
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
detergent
required
-
Lubrol Px
optimal concentration: 0.4%
Triton X-100
activates at 0.2%
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.35
Galbeta(1-3)GalNAcbeta(1-4)(NeuAcalpha(2-3))Galbeta(1-4)Glcbeta(1-1)ceramide
pH 3, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0000747
37°C, pH 3.0, 0.2% (w/v) Triton X-100, with Galbeta(1,3)GalNAcbeta(1,4)[NeuAcalpha(2,3)]Galbeta(1,4)Glcbeta-1,1'-ceramide as substrate
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3
-
transglycosylation reaction in the presence of 25% methanol
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.61
calculated from amino acid sequence
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
EGCSE_CYANO
503
1
57253
Swiss-Prot
Secretory Pathway (Reliability: 1)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
51000
gel filtration
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 51000, SDS-PAGE
monomer
1 * 51000, SDS-PAGE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
glycoprotein
enzyme contains 6 potential N-glycosylation sites
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
EGCase and EGCase P
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
EGCase and EGCase P
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Horibata, Y.; Higashi, H.; Ito, M.
Transglycosylation and reverse hydrolysis reactions of endoglycoceramidase from the jellyfish, Cyanea nozakii
J. Biochem.
130
263-268
2001
Cyanea nozakii
Manually annotated by BRENDA team
Horibata, Y.; Okino, N.; Ichinose, S.; Omori, A.; Ito, M.
Purification, characterization, and cDNA cloning of a novel acidic endoglycoceramidase from the jellyfish, Cyanea nozakii
J. Biol. Chem.
275
31297-31304
2000
Cyanea nozakii (Q9GV16), Cyanea nozakii
Manually annotated by BRENDA team
Horibata, Y.; Sakaguchi, K.; Okino, N.; Iida, H.; Inagaki, M.; Fujisawa, T.; Hama, Y.; Ito, M.
Unique catabolic pathway of glycosphingolipids in a hydrozoan, Hydra magnipapillata, involving endoglycoceramidase
J. Biol. Chem.
279
33379-33389
2004
Aiptasia sp., Bacillus sp. (in: Bacteria), Cellana sp., Cyanea nozakii (Q9GV16), Entacmaea sp., Hydra vulgaris, Hydra vulgaris 105, Marphysa sp., Meretrix sp., Ruditapes sp.
Manually annotated by BRENDA team