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Information on EC 3.2.1.122 - maltose-6'-phosphate glucosidase and Organism(s) Klebsiella pneumoniae and UniProt Accession Q9AGA6

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IUBMB Comments
Hydrolyses a variety of 6-phospho-alpha-D-glucosides, including alpha-maltose 6'-phosphate, alpha,alpha-trehalose 6-phosphate, sucrose 6-phosphate and p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate (as a chromogenic substrate). The enzyme is activated by FeII, MnII, CoII and NiII. It is rapidly inactivated in air.
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This record set is specific for:
Klebsiella pneumoniae
UNIPROT: Q9AGA6
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The taxonomic range for the selected organisms is: Klebsiella pneumoniae
The enzyme appears in selected viruses and cellular organisms
Synonyms
6-phosphoryl-o-alpha-d-glucopyranosyl:phosphoglucohydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phospho-alpha-glucosidase
-
6-phospho-alpha-D-glucosidase
-
-
-
-
6-phospho-alpha-glucosidase
-
-
-
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6-phosphoryl-O-alpha-D-glucopyranosyl:6phosphoglucohydrolase
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-
-
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6-phosphoryl-O-alpha-D-glucopyranosyl:phosphoglucohydrolase
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-
-
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maltose 6P hydrolase
-
-
-
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phospho-alpha-glucosidase
-
-
-
-
additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
maltose-6'-phosphate 6-phosphoglucohydrolase
Hydrolyses a variety of 6-phospho-alpha-D-glucosides, including alpha-maltose 6'-phosphate, alpha,alpha-trehalose 6-phosphate, sucrose 6-phosphate and p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate (as a chromogenic substrate). The enzyme is activated by FeII, MnII, CoII and NiII. It is rapidly inactivated in air.
CAS REGISTRY NUMBER
COMMENTARY hide
98445-08-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose + H2O
?
show the reaction diagram
-
-
-
?
isomaltose 6'-phosphate + H2O
alpha-D-glucopyranosyl 6-phosphate + D-glucose
show the reaction diagram
-
-
-
?
leucrose 6'-phosphate + H2O
?
show the reaction diagram
-
-
-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
?
maltitol 6-phosphate + H2O
alpha-D-glucopyranosyl 6-phosphate + D-glucitol
show the reaction diagram
-
-
-
?
maltose 6'-phosphate + H2O
?
show the reaction diagram
-
-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
broad substrate specificity
-
?
maltulose 6'-phosphate + H2O
?
show the reaction diagram
-
-
-
?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
?
methyl-alpha-D-glucopyranoside + H2O
methanol + alpha-D-glucose
show the reaction diagram
-
-
-
?
p-nitrophenyl alpha-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + alpha-D-glucose 6-phosphate
show the reaction diagram
-
-
-
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
palatinose 6'-phosphate + H2O
?
show the reaction diagram
-
-
-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
?
trehalose 6-phosphate + H2O
?
show the reaction diagram
-
-
-
?
trehalulose 6'-phosphate + H2O
?
show the reaction diagram
-
-
-
?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
?
turanose 6'-phosphate + H2O
?
show the reaction diagram
-
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1.68
3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose
pH 7.5
2.42
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose
pH 7.5
4.48
isomaltose 6'-phosphate
pH 7.5
5.63
leucrose 6'-phosphate
pH 7.5
0.82
maltitol 6-phosphate
pH 7.5
3.08
maltose 6'-phosphate
pH 7.5
1.2
maltulose 6'-phosphate
pH 7.5
0.05
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate
pH 7.5
1.16
trehalose 6-phosphate
pH 7.5
1.23
trehalulose 6'-phosphate
pH 7.5
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0198
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on glucose
0.0203
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0225
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0246
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0259
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.027
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0276
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0289
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on maltose
0.0318
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.034
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0355
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0361
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0375
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0418
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0453
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0456
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0466
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0484
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0489
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0502
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0513
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0538
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0565
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.062
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.063
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0642
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0643
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.065
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0664
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.069
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.072
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0725
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.075
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.08
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0823
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.088
0.0997
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1019
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1045
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.1187
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.124
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.1363
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.1575
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1654
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
4.15
pH 7.5, recombinant AglB, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
additional information
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36
in 0.1 M Tris-HCl or HEPES buffer, pH 7.5, 0.1 mM NAD+, 1 mM Mn2+
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4
2 species with pI values of 5.4 and 5.6, analytical isoelectric focusing
5.69
theoretical pI deduced from the amino acid composition
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
AGLB_KLEPN
440
1
49255
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
about, gel filtration
49000
x * 49000, SDS-PAGE
49256
50000
2 * 50000, SDS-PAGE and Western blot analysis
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dimer
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
inclusion of 0.1 mM NAD+ and 1 mM Mn2+ in all buffers stabilizes during purification
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant AglB expressed in Escherichia coli TOP 10, 3.4fold
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
aglB gene is cloned, sequenced and expressed in Escherichia coli TOP 10
plasmids encoding either aglB alone (pAP1) or both aglA and aglB genes (pAP2) expressed in Escherichia coli MG-1655
structural organization of the putative alpha-glycoside operon
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pikis, A.; Immel, S.; Robrish, S.A.; Thompson, J.
Metabolism of sucrose and its five isomers by Fusobacterium mortiferum
Microbiology
148
843-852
2002
Escherichia coli (Q8XEI0), Fusobacterium mortiferum (O06901), Klebsiella pneumoniae (Q9AGA6)
Manually annotated by BRENDA team
Thompson, J.; Robrish, S.A.; Immel, S.; Lichtenthaler, F.W.; Hall, B.G.; Pikis, A.
Metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Participation and properties of sucrose-6-phosphate hydrolase and phospho-alpha-glucosidase
J. Biol. Chem.
276
37415-37425
2001
Klebsiella pneumoniae (Q9AGA6)
Manually annotated by BRENDA team
Pikis, A.; Hess, S.; Arnold, I.; Erni, B.; Thompson, J.
Genetic requirements for growth of Escherichia coli K12 on methyl-alpha-D-glucopyranoside and the five alpha-D-glucosyl-D-fructose isomers of sucrose
J. Biol. Chem.
281
17900-17908
2006
Klebsiella pneumoniae (Q9AGA6)
Manually annotated by BRENDA team