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Information on EC 3.2.1.122 - maltose-6'-phosphate glucosidase and Organism(s) Fusobacterium mortiferum and UniProt Accession O06901

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IUBMB Comments
Hydrolyses a variety of 6-phospho-alpha-D-glucosides, including alpha-maltose 6'-phosphate, alpha,alpha-trehalose 6-phosphate, sucrose 6-phosphate and p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate (as a chromogenic substrate). The enzyme is activated by FeII, MnII, CoII and NiII. It is rapidly inactivated in air.
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This record set is specific for:
Fusobacterium mortiferum
UNIPROT: O06901
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The taxonomic range for the selected organisms is: Fusobacterium mortiferum
The enzyme appears in selected viruses and cellular organisms
Synonyms
6-phosphoryl-o-alpha-d-glucopyranosyl:phosphoglucohydrolase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6-phospho-alpha-D-glucosidase
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6-phospho-alpha-glucosidase
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6-phosphoryl-O-alpha-D-glucopyranosyl:6phosphoglucohydrolase
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6-phosphoryl-O-alpha-D-glucopyranosyl:phosphoglucohydrolase
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maltose 6P hydrolase
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phospho-alpha-glucosidase
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additional information
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
maltose-6'-phosphate 6-phosphoglucohydrolase
Hydrolyses a variety of 6-phospho-alpha-D-glucosides, including alpha-maltose 6'-phosphate, alpha,alpha-trehalose 6-phosphate, sucrose 6-phosphate and p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate (as a chromogenic substrate). The enzyme is activated by FeII, MnII, CoII and NiII. It is rapidly inactivated in air.
CAS REGISTRY NUMBER
COMMENTARY hide
98445-08-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
show the reaction diagram
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
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-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
broad substrate specificity
molar ratio is 1:1
?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
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-
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
beta-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
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-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
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-
?
trehalose 6-phosphate + H2O
?
show the reaction diagram
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-
-
?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
show the reaction diagram
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-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
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-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
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molar ratio is 1:1, yellow product
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
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-
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?
sucrose 6-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
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hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio
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-
?
trehalose 6-phosphate + H2O
?
show the reaction diagram
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hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio
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-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
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?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
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in the pathway of maltose fermentation
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NAD+
cofactor, requirement
additional information
requires a divalent metal ion, e.g. Mn2+
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
requires Fe2+, Mn2+, Co2+ or Ni2+
Co2+
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activates
Fe2+
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activates
Mn2+
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activates
Ni2+
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activates
additional information
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
air
exposure to air rapidly inactivates
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air
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exposure to air rapidly inactivates
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sulfhydryl-reactive agent
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rapidly inactivates
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.9
maltose 6'-phosphate
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pH 7, 37°C
2
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate
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pH 7, 37°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2.9
pH 7.5, 37°C, recombinant MalH, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
1.67
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at room temperature, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
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maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
40
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maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
analytical isoelectric focusing
5.5
amino acid sequence
4.9
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analytical isoelectric focusing
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC 25557, MalH
SwissProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
MALH_FUSMR
441
1
49712
Swiss-Prot
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
49000
x * 49000, SDS-PAGE
49712
4 * 49712, calculated from the amino acid sequence
49718
x * 49718, calculated from the amino acid sequence
50000
4 * 50000, SDS-PAGE
49000
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x * 49000, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
?
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x * 49000, SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis and storage overnight results in 90% loss of activity
dialysis and storage overnight results in 90% loss of activity
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freezing and thawing from -20°C retains 50% of initial activity
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inclusion of dithiothreitol and Mn2+ in chromatography buffers stabilizes during purification
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purification by Rotofor isoelectric focusing inactivates
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
O2-sensitive enzyme, rapidly inactivated by exposure to air
646683
O2-sensitive enzyme, rapidly inactivated by exposure to air
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646682
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 25 mM HEPES buffer, pH 7.5, 1 mM Mn2+, 1 mM dithiothreitol, several weeks, stable
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storage overnight, 90% loss of activity
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant MalH expressed in Escherichia coli PEP43
6fold, dithiothreitol and Mn2+ is required for stabilization during purification
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
malH gene encodes maltose 6P hydrolase, a 441 residues protein, and is cloned, sequenced and expressed in Escherichia coli XLOLR
structural organization of the putative alpha-glycoside operon, gene malH is adjacent to malB and malR, malH gene is cloned and expressed in Escherichia coli PEP43
gene encoding maltose 6P hydrolase, a 441 residues protein, is cloned, sequenced and actively expressed in Escherichia coli
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Thompson, J.; Gentry-Weeks, C.R.; Nguyen, N.Y.; Folk, J.E.; Robrish, S.A.
Purification from Fusobacterium mortiferum ATCC 25557 of a 6-phosphoryl-O-alpha-D-glucopyranosyl:6-phosphoglucohydrolase that hydrolyzes maltose 6-phosphate and related phospho-alpha-D-glucosides
J. Bacteriol.
177
2505-2512
1995
Fusobacterium mortiferum
Manually annotated by BRENDA team
Bouma, C.L.; Reizer, J.; Reizer, A.; Robrish, S.A.; Thompson, J.
6-Phospho-alpha-D-glucosidase from Fusobacterium mortiferum: Cloning, expression, and assignment to family 4 of the glycosylhydrolases
J. Bacteriol.
179
4129-4137
1997
Fusobacterium mortiferum (O06901), Fusobacterium mortiferum
Manually annotated by BRENDA team
Pikis, A.; Immel, S.; Robrish, S.A.; Thompson, J.
Metabolism of sucrose and its five isomers by Fusobacterium mortiferum
Microbiology
148
843-852
2002
Escherichia coli (Q8XEI0), Fusobacterium mortiferum (O06901), Klebsiella pneumoniae (Q9AGA6)
Manually annotated by BRENDA team