Information on EC 3.2.1.122 - maltose-6'-phosphate glucosidase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY
3.2.1.122
-
RECOMMENDED NAME
GeneOntology No.
maltose-6'-phosphate glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
-
-
SYSTEMATIC NAME
IUBMB Comments
maltose-6'-phosphate 6-phosphoglucohydrolase
Hydrolyses a variety of 6-phospho-alpha-D-glucosides, including alpha-maltose 6'-phosphate, alpha,alpha-trehalose 6-phosphate, sucrose 6-phosphate and p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate (as a chromogenic substrate). The enzyme is activated by FeII, MnII, CoII and NiII. It is rapidly inactivated in air.
SYNONYMS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6-phospho-alpha-D-glucosidase
-
-
-
-
6-phospho-alpha-glucosidase
-
-
-
-
6-phospho-alpha-glucosidase
-
-
6-phospho-alpha-glucosidase
P54716
-
6-phospho-alpha-glucosidase
-
-
6-phosphoryl-O-alpha-D-glucopyranosyl:6phosphoglucohydrolase
-
-
-
-
6-phosphoryl-O-alpha-D-glucopyranosyl:phosphoglucohydrolase
-
-
-
-
GlvA
Bacillus subtilis GlvA
-
-
-
GlvG
Q8XEI0
-
phospho-alpha-glucosidase
-
-
-
-
phospho-alpha-glucosidase
P54716
-
phospho-alpha-glucosidase
Q9AGA6
-
maltose 6P hydrolase
-
-
-
-
additional information
Q97LM4
belongs to familiy 4 of the glycosylhydrolase superfamily
additional information
O06901
belongs to familiy 4 of the glycosylhydrolase superfamily
additional information
Q9AGA6
belongs to familiy 4 of the glycosylhydrolase superfamily
CAS REGISTRY NUMBER
COMMENTARY
98445-08-0
-
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Bacillus subtilis GlvA
GlvA
-
-
Manually annotated by BRENDA team
strain ATCC 824, MalH, i.e. maltose 6-phosphate hydrolase
GenBank
Manually annotated by BRENDA team
strain ATCC 824, PagL, i.e. phospho-alpha-glucosidase
GenBank
Manually annotated by BRENDA team
enterohaemorrhagic strain O157:H7, GlvG
GenBank
Manually annotated by BRENDA team
strain ATCC 25557
-
-
Manually annotated by BRENDA team
strain ATCC 25557, MalH
SwissProt
Manually annotated by BRENDA team
strain ATCC 23357, AglB
SwissProt
Manually annotated by BRENDA team
strain OMZ 176
-
-
Manually annotated by BRENDA team
Streptococcus mutans OMZ 176
strain OMZ 176
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-methyl-alpha-glucopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside + H2O
3,4-dinitrophenol + alpha-D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
show the reaction diagram
Q97DP6, Q97LM4
-
-
-
?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
show the reaction diagram
O06901
-
fluorescent product
?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
show the reaction diagram
-
-
fluorescent product
?
4-nitrophenyl 6-phospho-alpha-D-glucoside + H2O
4-nitrophenol + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
6-phospho-beta-D-glucose + H2O
phosphate + D-glucose
show the reaction diagram
-
substrate analog
-
-
?
isomaltose 6'-phosphate + H2O
alpha-D-glucopyranosyl 6-phosphate + D-glucose
show the reaction diagram
-
-
-
-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
Q9AGA6
-
-
-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
Q97DP6, Q97LM4
-
-
-
?
leucrose 6'-phosphate + H2O
?
show the reaction diagram
Q9AGA6
-
-
-
?
maltitol 6-phosphate + H2O
alpha-D-glucopyranosyl 6-phosphate + D-glucitol
show the reaction diagram
-
-
-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
Q97DP6, Q97LM4
-
-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
broad substrate specificity
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
broad substrate specificity
molar ratio is 1:1
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
O06901
broad substrate specificity
molar ratio is 1:1
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
involved in maltose glycolysis, enzyme is induced by maltose
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
in the pathway of maltose fermentation
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
Streptococcus mutans OMZ 176
-
involved in maltose glycolysis, enzyme is induced by maltose
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
Bacillus subtilis GlvA
-
enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
-
?
maltose 6'-phosphate + H2O
D-glucose + D-glucose 6-phosphate
show the reaction diagram
P54716
-
-
-
?
maltose 6'-phosphate + H2O
?
show the reaction diagram
Q9AGA6
-
-
-
?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
Q9AGA6
-
-
-
?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
-
?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
Q97DP6, Q97LM4
-
-
-
?
maltulose 6'-phosphate + H2O
?
show the reaction diagram
Q9AGA6
-
-
-
?
methyl 6-phospho-alpha-D-glucoside + H2O
methanol + alpha-D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
p-nitrophenyl alpha-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + alpha-D-glucose 6-phosphate
show the reaction diagram
Q9AGA6
-
-
-
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
-
-
molar ratio is 1:1, yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
O06901
-
yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
-
-
yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
Q9AGA6
-
yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
-
-
yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
Q97DP6, Q97LM4
MalH and PagL, Cys-169 and Asp-170 are essential for hydrolysis
yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
Q97DP6, Q97LM4
only substrate
yellow product
?
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
beta-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
-
-
-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
Q9AGA6
-
-
-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
Q97DP6, Q97LM4
-
-
-
?
palatinose 6'-phosphate + H2O
?
show the reaction diagram
Q9AGA6
-
-
-
?
phenyl 6-phospho-alpha-D-glucoside + H2O
phenol + alpha-D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
sucrose 6-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio
-
-
?
trehalose 6-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
trehalose 6-phosphate + H2O
?
show the reaction diagram
O06901
-
-
-
?
trehalose 6-phosphate + H2O
?
show the reaction diagram
-
-
-
-
?
trehalose 6-phosphate + H2O
?
show the reaction diagram
-
hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio
-
-
?
trehalose 6-phosphate + H2O
?
show the reaction diagram
Bacillus subtilis GlvA
-
-
-
-
?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
show the reaction diagram
Q9AGA6
-
-
-
?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
-
?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
show the reaction diagram
Q97DP6, Q97LM4
-
-
-
?
trehalulose 6'-phosphate + H2O
?
show the reaction diagram
Q9AGA6
-
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
Q9AGA6
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
Q97DP6, Q97LM4
-
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
-
-
molar ratio is 1:1
?
turanose 6'-phosphate + H2O
?
show the reaction diagram
Q9AGA6
-
-
-
?
methyl-alpha-D-glucopyranoside + H2O
methanol + alpha-D-glucose
show the reaction diagram
Q9AGA6
-
-
-
?
additional information
?
-
Q9AGA6
-
-
-
-
additional information
?
-
Q97DP6, Q97LM4
not: methyl-beta-glucose 6-phosphate
-
-
-
additional information
?
-
-
not: cellobiose 6-phosphate, gentiobiose 6-phosphate
-
-
-
additional information
?
-
Q97DP6, Q97LM4
not: cellobiose 6-phosphate, gentiobiose 6-phosphate
-
-
-
additional information
?
-
-
not: D-glucose, D-fructose, non-phosphorylated sucrose isomers
-
-
-
additional information
?
-
-
not: nonphosphorylated alpha- and beta-glycosides, p-nitrophenyl-beta-D-glucopyranoside 6-phosphate, p-nitrophenyl-alpha-D-mannopyranoside 6-phosphate, very poor substrate: p-nitrophenyl-alpha-D-galactopyranoside 6-phosphate
-
-
-
additional information
?
-
Q97DP6, Q97LM4
only MalH catalyzes the hydrolysis of disaccharide 6-phosphates formed via the phosphoenolpyruvate-dependent:sugar phosphotransferase system, not PagL
-
-
-
additional information
?
-
-
substrate specificity, not: sucrose 6-phosphate
-
-
-
additional information
?
-
Q9AGA6
substrate specificity, not: sucrose 6-phosphate
-
-
-
additional information
?
-
-
substrate specificity, not: sucrose 6-phosphate
-
-
-
additional information
?
-
Q97DP6, Q97LM4
substrate specificity, not: sucrose 6-phosphate
-
-
-
additional information
?
-
O06901
carbohydrate metabolism
-
-
-
additional information
?
-
-
carbohydrate metabolism
-
-
-
additional information
?
-
-
AglB and AglA, an enzyme II(CB) component of the PEP:PTS, facilitate the phosphorylative translocation and subsequent cleavage of phosphorylated isomers of sucrose and related alpha-glycosides
-
-
-
additional information
?
-
Q97DP6, Q97LM4
MalH participates in dissimilation of maltose and sucrose isomers, PagL not
-
-
-
additional information
?
-
Q9AGA6
sucrose 6'-phosphate is not a substrate for AglB
-
-
-
additional information
?
-
-
6-phospho-alpha-glycosides are the preferred substrates of GlvA
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
involved in maltose glycolysis, enzyme is induced by maltose
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
-
in the pathway of maltose fermentation
-
?
maltose 6'-phosphate + H2O
D-glucose + D-glucose 6-phosphate
show the reaction diagram
P54716
-
-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
Streptococcus mutans OMZ 176
-
involved in maltose glycolysis, enzyme is induced by maltose
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
Bacillus subtilis GlvA
-
enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
-
?
additional information
?
-
Q9AGA6
-
-
-
-
additional information
?
-
O06901
carbohydrate metabolism
-
-
-
additional information
?
-
-
carbohydrate metabolism
-
-
-
additional information
?
-
-
AglB and AglA, an enzyme II(CB) component of the PEP:PTS, facilitate the phosphorylative translocation and subsequent cleavage of phosphorylated isomers of sucrose and related alpha-glycosides
-
-
-
additional information
?
-
Q97DP6, Q97LM4
MalH participates in dissimilation of maltose and sucrose isomers, PagL not
-
-
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
-
cofactor, requirement
NAD+
-
NAD+-dependent
NAD+
Q97DP6, Q97LM4
NAD+-dependent; NAD+-dependent
NAD+
-
dependent on NAD+, the glycosidic bond hydrolysis is initiated by oxidation of the glucopyranose ring at the 3-position to a keto group by the NAD+ molecule bound to the active site
NADH
-
requirement
additional information
-
requires a divalent metal ion, e.g. Mn2+
-
additional information
-
requires a divalent metal ion, e.g. Mn2+
-
additional information
-
not activated by NADH
-
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
-
requires Fe2+, Mn2+, Co2+ or Ni2+
Co2+
-
requires Mn2+, Co2+ or Ni2+
Fe2+
-
activates
Fe2+
-
requires Fe2+, Mn2+, Co2+ or Ni2+
Mn2+
-
requirement, permits formation of the active tetramer of GlvA from its inactive dimeric state
Mn2+
-
activates
Mn2+
-
requires Fe2+, Mn2+, Co2+ or Ni2+
Mn2+
-
cofactor, requirement
Mn2+
-
requires Mn2+, Co2+ or Ni2+
Mn2+
Q97DP6, Q97LM4
cofactor, requirement; cofactor, requirement
Mn2+
-
essential for activity
Ni2+
-
requires Fe2+, Mn2+, Co2+ or Ni2+
Ni2+
-
requires Mn2+, Co2+ or Ni2+
Mn2+
-
required
additional information
-
requires a divalent metal ion, e.g. Mn2+
additional information
-
metal-dependent; not activated by Mg2+, Zn2+ or Ca2+; not activated by Sr2+
additional information
-
metal-dependent; requires a divalent metal ion, e.g. Mn2+
additional information
-
metal-dependent
additional information
-
metal-dependent; not activated by Mg2+, Zn2+ or Ca2+
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
air
-
exposure to air rapidly inactivates
-
cellobiose 6'-phosphate
-
competitive inhibitor
maltotriitol
-
strong
methyl 6-phospho-alpha-D-glucoside
-
competitive inhibitor
sulfhydryl-reactive agent
-
rapidly inactivates
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
dithiothreitol
Q97DP6, Q97LM4
dithiothreitol-dependent; dithiothreitol-dependent
additional information
-
growth on alpha-glycosides, such as maltose, alpha-methylglycoside, trehalose, turanose and palatinose, increases dramatically enzyme synthesis
-
additional information
-
MalH is induced during growth on the sucrose isomers trehalulose, turanose, maltulose, leucrose and palatinose, but not on sucrose
-
additional information
Q97DP6, Q97LM4
differential expression of MalH and PagL during growth on various sugars, enzyme induction, nucleotide specificity for activation of MalH and PagL; differential expression of MalH and PagL during growth on various sugars, enzyme induction, nucleotide specificity for activation of MalH and PagL
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.004
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
-
at 37C in Tris-HCl buffer, pH 8.4
0.012
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
-
at 37C in HEPES buffer, pH 7.5
0.82
3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose
Q97DP6, Q97LM4
pH 7, 37C
1.68
3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose
-
pH 7.5
0.014
4-nitrophenyl 6-phospho-alpha-D-glucoside
-
at 37C in Tris-HCl buffer, pH 8.4
0.052
4-nitrophenyl 6-phospho-alpha-D-glucoside
-
at 37C in HEPES buffer, pH 7.5
2.42
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose
-
pH 7.5
2.47
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose
Q97DP6, Q97LM4
pH 7, 37C
4.48
isomaltose 6'-phosphate
-
pH 7.5
1.87
leucrose 6'-phosphate
Q97DP6, Q97LM4
pH 7, 37C
5.63
leucrose 6'-phosphate
-
pH 7.5
0.82
maltitol 6-phosphate
-
pH 7.5
0.36
maltose 6'-phosphate
-
at 37C in Tris-HCl buffer, pH 8.4
0.4
maltose 6'-phosphate
-
at 37C in HEPES buffer, pH 7.5
0.9
maltose 6'-phosphate
-
pH 7, 37C
1.95
maltose 6'-phosphate
Q97DP6, Q97LM4
pH 7, 37C
3.08
maltose 6'-phosphate
-
pH 7.5
1.11
maltulose 6'-phosphate
Q97DP6, Q97LM4
pH 7, 37C
1.2
maltulose 6'-phosphate
-
pH 7.5
0.5
methyl 6-phospho-alpha-D-glucoside
-
at 37C in Tris-HCl buffer, pH 8.4
0.61
methyl 6-phospho-alpha-D-glucoside
-
at 37C in HEPES buffer, pH 7.5
0.028
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate
Q97DP6, Q97LM4
pH 7, 37C
-
0.05
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate
-
pH 7.5
-
0.058
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate
Q97DP6, Q97LM4
pH 7, 37C
-
2
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate
-
pH 7, 37C
-
0.038
phenyl 6-phospho-alpha-D-glucoside
-
at 37C in Tris-HCl buffer, pH 8.4
0.069
phenyl 6-phospho-alpha-D-glucoside
-
at 37C in HEPES buffer, pH 7.5
1.16
Trehalose 6-phosphate
-
pH 7.5
1.23
trehalulose 6'-phosphate
-
pH 7.5
1.92
trehalulose 6'-phosphate
Q97DP6, Q97LM4
pH 7, 37C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.72
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
-
at 37C in HEPES buffer, pH 7.5
0.81
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
-
at 37C in Tris-HCl buffer, pH 8.4
0.7
4-nitrophenyl 6-phospho-alpha-D-glucoside
-
at 37C in HEPES buffer, pH 7.5
0.85
4-nitrophenyl 6-phospho-alpha-D-glucoside
-
at 37C in Tris-HCl buffer, pH 8.4
1.3
maltose 6'-phosphate
-
at 37C in HEPES buffer, pH 7.5; at 37C in Tris-HCl buffer, pH 8.4
0.42
methyl 6-phospho-alpha-D-glucoside
-
at 37C in HEPES buffer, pH 7.5
1.1
methyl 6-phospho-alpha-D-glucoside
-
at 37C in Tris-HCl buffer, pH 8.4
0.26
phenyl 6-phospho-alpha-D-glucoside
-
at 37C in Tris-HCl buffer, pH 8.4
0.9
phenyl 6-phospho-alpha-D-glucoside
-
at 37C in HEPES buffer, pH 7.5
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.03
cellobiose 6'-phosphate
-
-
0.075
methyl 6-phospho-alpha-D-glucoside
-
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0198
Q9AGA6
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on glucose
0.0203
Q9AGA6
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0225
Q9AGA6
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0246
Q9AGA6
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0259
Q9AGA6
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.027
Q9AGA6
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0276
Q9AGA6
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0289
Q9AGA6
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on maltose
0.0318
Q9AGA6
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.034
Q9AGA6
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0355
Q9AGA6
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0361
Q9AGA6
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0375
Q9AGA6
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0418
Q9AGA6
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0453
Q9AGA6
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0456
Q9AGA6
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0466
Q9AGA6
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0484
Q9AGA6
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0489
Q9AGA6
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0502
Q9AGA6
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0513
Q9AGA6
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0538
Q9AGA6
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0565
Q9AGA6
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.062
Q9AGA6
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.063
Q9AGA6
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0642
Q9AGA6
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0643
Q9AGA6
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.065
Q9AGA6
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0664
Q9AGA6
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.069
Q9AGA6
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.072
Q9AGA6
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0725
Q9AGA6
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.075
Q9AGA6
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.08
Q9AGA6
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0823
Q9AGA6
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.088
Q9AGA6
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose; with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0997
Q9AGA6
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1019
Q9AGA6
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1045
Q9AGA6
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.1187
Q9AGA6
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.124
Q9AGA6
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.1363
Q9AGA6
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.1575
Q9AGA6
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1654
Q9AGA6
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
1.67
-
at room temperature, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
2.9
-
pH 7.5, 37C, recombinant MalH, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
4.15
-
pH 7.5, recombinant AglB, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
4.9
Q97DP6, Q97LM4
pH 7.5, 37C, recombinant MalH, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
additional information
-
-
additional information
-
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 7.5
-
maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
7.5
Q9AGA6
assay at
7.5
Q97DP6, Q97LM4
assay at; assay at
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
36
-
in 0.1 M Tris-HCl or HEPES buffer, pH 7.5, 0.1 mM NAD+, 1 mM Mn2+
37
Q97DP6, Q97LM4
assay at; assay at
40
-
maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.9
-
analytical isoelectric focusing
5.4
-
2 species with pI values of 5.4 and 5.6, analytical isoelectric focusing
5.5
-
amino acid sequence
5.69
-
theoretical pI deduced from the amino acid composition
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
Bacillus subtilis (strain 168)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
100000
-
about, gel filtration
646685
200000
Q97DP6, Q97LM4
about, MalH and PagL, HPLC gel filtration; about, MalH and PagL, HPLC gel filtration
646686
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
?
-
x * 49000, SDS-PAGE
?
Q9AGA6
x * 49000, SDS-PAGE
?
-
x * 49718, calculated from the amino acid sequence
?
-
x * 49256, calculated from the amino acid sequence
?
Q8XEI0
x * 49386, calculated from the amino acid sequence
dimer
-
inactive state
dimer
-
catalytically active homodimer, 2 * 49256, calculated from the amino acid sequence, 2 * 50000, SDS-PAGE and Western blot analysis
dimer
Bacillus subtilis GlvA
-
inactive state
-
tetramer
-
4 * 50000, SDS-PAGE
tetramer
Q97DP6, Q97LM4
4 * 51155, recombinant PagL, predicted from the amino acid sequence
tetramer
-
4 * 50513, native GlvA is active as tetramer
tetramer
Q97DP6, Q97LM4
4 * 49972, recombinant MalH, predicted from the amino acid sequence
tetramer
Q97DP6, Q97LM4
4 * 50038, recombinant MalH, electrospray ionization mass spectrometry
tetramer
Q97DP6, Q97LM4
4 * 51205, recombinant PagL, electrospray ionization mass spectrometry
tetramer
-
4 * 50000, recombinant MalH and PagL, SDS-PAGE
tetramer
-
4 * 49712, calculated from the amino acid sequence
tetramer
-
alpha4, crystal structure
tetramer
Bacillus subtilis GlvA
-
4 * 50513, native GlvA is active as tetramer
-
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in the presence and absence of its known ligands, resolution at 2.05 A
-
recombinant GlvA expressed in Escherichia coli, vapour-phase diffusion using the hanging-drop method, NAD/Mn2+-dependent crystal form
-
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis and storage overnight results in 90% loss of activity
-
freezing and thawing from -20C retains 50% of initial activity
-
inclusion of dithiothreitol and Mn2+ in chromatography buffers stabilizes during purification
-
purification by Rotofor isoelectric focusing inactivates
-
unstable enzyme
-
inclusion of 0.1 mM NAD+ and 1 mM Mn2+ in all buffers stabilizes during purification
-
OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
O2-sensitive enzyme, rapidly inactivated by exposure to air
-
646682, 646683
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 25 mM HEPES buffer, pH 7.5, 1 mM Mn2+, 1 mM dithiothreitol, several weeks, stable
-
storage overnight, 90% loss of activity
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme
-
recombinant GlvA expressed in Escherichia coli
-
recombinant MalH expressed in Escherichia coli PEP43; recombinant PagL expressed in Escherichia coli PEP43
Q97DP6, Q97LM4
6fold, dithiothreitol and Mn2+ is required for stabilization during purification
-
recombinant MalH expressed in Escherichia coli PEP43
-
recombinant AglB expressed in Escherichia coli TOP 10, 3.4fold
-
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
-
glvA gene, formerly glv1, is encoded within the maltose PEP-PTS operon, it is cloned and overexpressed in Escherichia coli, glvA encodes a 449 amino acid protein
-
genes malh and pagl encoding maltose 6-phosphate hydrolase MalH and phospho-alpha-glucosidase PagL are cloned, sequenced and expressed in Escherichia coli PEP43, the 2 genes reside in separate operons, molecular organization of the operons mal and pagl; genes malh and pagl encoding maltose 6-phosphate hydrolase MalH and phospho-alpha-glucosidase PagL are cloned, sequenced and expressed in Escherichia coli PEP43, the 2 genes reside in separate operons, molecular organization of the operons mal and pagl
Q97DP6, Q97LM4
structural organization of the putative alpha-glycoside operon
Q8XEI0
gene encoding maltose 6P hydrolase, a 441 residues protein, is cloned, sequenced and actively expressed in Escherichia coli
-
malH gene encodes maltose 6P hydrolase, a 441 residues protein, and is cloned, sequenced and expressed in Escherichia coli XLOLR
-
structural organization of the putative alpha-glycoside operon, gene malH is adjacent to malB and malR, malH gene is cloned and expressed in Escherichia coli PEP43
-
aglB gene is cloned, sequenced and expressed in Escherichia coli TOP 10; structural organization of the putative alpha-glycoside operon
-
plasmids encoding either aglB alone (pAP1) or both aglA and aglB genes (pAP2) expressed in Escherichia coli MG-1655
Q9AGA6
structural organization of the putative alpha-glycoside operon
-
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
C169S
Q97DP6, Q97LM4
catalytically inactive MalH mutant
D170N
Q97DP6, Q97LM4
catalytically inactive MalH mutant
M171V
Q97DP6, Q97LM4
MalH mutant with reduced activity
P172A
Q97DP6, Q97LM4
MalH mutant with reduced activity