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Information on EC 3.2.1.122 - maltose-6'-phosphate glucosidase
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EC Tree
3.2.1.122 maltose-6'-phosphate glucosidaseIUBMB Comments
Hydrolyses a variety of 6-phospho-alpha-D-glucosides, including alpha-maltose 6'-phosphate, alpha,alpha-trehalose 6-phosphate, sucrose 6-phosphate and p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate (as a chromogenic substrate). The enzyme is activated by FeII, MnII, CoII and NiII. It is rapidly inactivated in air.
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The enzyme appears in viruses and cellular organisms
Synonyms
6-phosphoryl-o-alpha-d-glucopyranosyl:phosphoglucohydrolase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
6-phospho-alpha-D-glucosidase
-
-
-
-
6-phospho-alpha-glucosidase
6-phosphoryl-O-alpha-D-glucopyranosyl:6phosphoglucohydrolase
-
-
-
-
6-phosphoryl-O-alpha-D-glucopyranosyl:phosphoglucohydrolase
-
-
-
-
GlvA
MalH
maltose 6P hydrolase
-
-
-
-
maltose-6'-phosphate phosphatase
mapP
phospho-alpha-glucosidase
additional information
6-phospho-alpha-glucosidase
-
-
-
-
phospho-alpha-glucosidase
-
-
-
-
additional information
belongs to familiy 4 of the glycosylhydrolase superfamily
additional information
belongs to familiy 4 of the glycosylhydrolase superfamily
additional information
belongs to familiy 4 of the glycosylhydrolase superfamily
additional information
belongs to familiy 4 of the glycosylhydrolase superfamily
additional information
belongs to familiy 4 of the glycosylhydrolase superfamily
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
maltose-6'-phosphate 6-phosphoglucohydrolase
Hydrolyses a variety of 6-phospho-alpha-D-glucosides, including alpha-maltose 6'-phosphate, alpha,alpha-trehalose 6-phosphate, sucrose 6-phosphate and p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate (as a chromogenic substrate). The enzyme is activated by FeII, MnII, CoII and NiII. It is rapidly inactivated in air.
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CAS REGISTRY NUMBER
COMMENTARY
98445-08-0
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside + H2O
3,4-dinitrophenol + alpha-D-glucose 6-phosphate
-
-
-
-
?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
4-nitrophenyl 6-phospho-alpha-D-glucoside + H2O
4-nitrophenol + alpha-D-glucose
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
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-
-
-
?
6-phospho-beta-D-glucose + H2O
phosphate + D-glucose
substrate analog
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-
?
isomaltose 6'-phosphate + H2O
alpha-D-glucopyranosyl 6-phosphate + D-glucose
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-
-
?
maltitol 6-phosphate + H2O
alpha-D-glucopyranosyl 6-phosphate + D-glucitol
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-
-
?
maltose 6,6'-bisphosphate + H2O
D-glucose 6-phosphate + D-fructose
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6.0% activity compared to maltose 6'-phosphate
-
-
?
methyl 6-phospho-alpha-D-glucoside + H2O
methanol + alpha-D-glucose 6-phosphate
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-
-
-
?
methyl-alpha-D-glucopyranoside + H2O
methanol + alpha-D-glucose
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-
-
?
p-nitrophenyl alpha-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + alpha-D-glucose 6-phosphate
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-
-
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
beta-D-glucose 6-phosphate + p-nitrophenol
-
-
?
phenyl 6-phospho-alpha-D-glucoside + H2O
phenol + alpha-D-glucose 6-phosphate
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-
-
-
?
sucrose 6-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio
-
-
?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
-
-
-
?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
-
fluorescent product
?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
-
fluorescent product
?
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose + H2O
?
-
-
-
?
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose + H2O
?
-
-
-
?
esculin 6-phosphate + H2O
?
-
0.6% activity compared to maltose 6'-phosphate
-
-
?
esculin 6-phosphate + H2O
?
-
0.6% activity compared to maltose 6'-phosphate
-
-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
-
-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
18.8% activity compared to maltose 6'-phosphate
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-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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-
-
?
maltose 6'-phosphate + H2O
D-glucose + D-glucose 6-phosphate
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-
-
?
maltose 6'-phosphate + H2O
D-glucose + D-glucose 6-phosphate
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100% activity
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-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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broad substrate specificity
molar ratio is 1:1
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
broad substrate specificity
molar ratio is 1:1
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
in the pathway of maltose fermentation
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
broad substrate specificity
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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involved in maltose glycolysis, enzyme is induced by maltose
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?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
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involved in maltose glycolysis, enzyme is induced by maltose
-
?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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-
-
?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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8.1% activity compared to maltose 6'-phosphate
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-
?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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-
?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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-
-
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
MalH and PagL, Cys-169 and Asp-170 are essential for hydrolysis
yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
only substrate
yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
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molar ratio is 1:1, yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
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yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
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yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
-
yellow product
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
-
yellow product
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
-
-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
0.9% activity compared to maltose 6'-phosphate
-
-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
0.9% activity compared to maltose 6'-phosphate
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-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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-
-
-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
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-
-
?
trehalose 6-phosphate + H2O
?
-
hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio
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-
?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
-
-
-
?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
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2.1% activity compared to maltose 6'-phosphate
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-
?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
-
-
?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
-
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
85% activity compared to maltose 6'-phosphate
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-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
85% activity compared to maltose 6'-phosphate
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
-
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
-
molar ratio is 1:1
?
additional information
?
-
6-phospho-alpha-glycosides are the preferred substrates of GlvA
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-
?
additional information
?
-
not: methyl-beta-glucose 6-phosphate
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-
?
additional information
?
-
not: methyl-beta-glucose 6-phosphate
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-
?
additional information
?
-
not: cellobiose 6-phosphate, gentiobiose 6-phosphate
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-
?
additional information
?
-
not: cellobiose 6-phosphate, gentiobiose 6-phosphate
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-
?
additional information
?
-
only MalH catalyzes the hydrolysis of disaccharide 6-phosphates formed via the phosphoenolpyruvate-dependent:sugar phosphotransferase system, not PagL
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-
?
additional information
?
-
only MalH catalyzes the hydrolysis of disaccharide 6-phosphates formed via the phosphoenolpyruvate-dependent:sugar phosphotransferase system, not PagL
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-
?
additional information
?
-
substrate specificity, not: sucrose 6-phosphate
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-
?
additional information
?
-
substrate specificity, not: sucrose 6-phosphate
-
-
?
additional information
?
-
MalH participates in dissimilation of maltose and sucrose isomers, PagL not
-
-
?
additional information
?
-
MalH participates in dissimilation of maltose and sucrose isomers, PagL not
-
-
?
additional information
?
-
-
4-nitrophenyl-alpha-D-glucopyranoside 6-phosphate, cellobiose 6'-phosphate, thiocellobiose 6'-phosphate, cellobiitol 6'-phosphate, beta-methylglucoside 6-phosphate, salicin 6-phosphate, laminaribose 6'-phosphate, gentiobiose 6'-phosphate, arbutin 6-phosphate, sucrose 6-phosphate, D-glucose 6-phosphate, D-fructose 6-phosphate and bet-methylglucoside 6-phosphate are not dephosphorylated by the enzyme
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-
?
additional information
?
-
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the enzyme preferably dephosphorylates disaccharides containing an O-alpha-glycosyl linkage
-
-
?
additional information
?
-
-
4-nitrophenyl-alpha-D-glucopyranoside 6-phosphate, cellobiose 6'-phosphate, thiocellobiose 6'-phosphate, cellobiitol 6'-phosphate, beta-methylglucoside 6-phosphate, salicin 6-phosphate, laminaribose 6'-phosphate, gentiobiose 6'-phosphate, arbutin 6-phosphate, sucrose 6-phosphate, D-glucose 6-phosphate, D-fructose 6-phosphate and bet-methylglucoside 6-phosphate are not dephosphorylated by the enzyme
-
-
?
additional information
?
-
-
the enzyme preferably dephosphorylates disaccharides containing an O-alpha-glycosyl linkage
-
-
?
additional information
?
-
not: cellobiose 6-phosphate, gentiobiose 6-phosphate
-
-
?
additional information
?
-
not: D-glucose, D-fructose, non-phosphorylated sucrose isomers
-
-
?
additional information
?
-
-
not: nonphosphorylated alpha- and beta-glycosides, p-nitrophenyl-beta-D-glucopyranoside 6-phosphate, p-nitrophenyl-alpha-D-mannopyranoside 6-phosphate, very poor substrate: p-nitrophenyl-alpha-D-galactopyranoside 6-phosphate
-
-
?
additional information
?
-
substrate specificity, not: sucrose 6-phosphate
-
-
?
additional information
?
-
substrate specificity, not: sucrose 6-phosphate
-
-
?
additional information
?
-
substrate specificity, not: sucrose 6-phosphate
-
-
?
additional information
?
-
AglB and AglA, an enzyme II(CB) component of the PEP:PTS, facilitate the phosphorylative translocation and subsequent cleavage of phosphorylated isomers of sucrose and related alpha-glycosides
-
-
?
additional information
?
-
sucrose 6'-phosphate is not a substrate for AglB
-
-
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
maltose 6'-phosphate + H2O
D-glucose + D-glucose 6-phosphate
-
-
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
enzyme participates in the catabolism of phosphorylated alpha-glycosides accumulated via a phosphoenolpyruvate-dependent maltose phosphotransferase system
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
in the pathway of maltose fermentation
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
involved in maltose glycolysis, enzyme is induced by maltose
-
?
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
-
involved in maltose glycolysis, enzyme is induced by maltose
-
?
additional information
?
-
MalH participates in dissimilation of maltose and sucrose isomers, PagL not
-
-
?
additional information
?
-
MalH participates in dissimilation of maltose and sucrose isomers, PagL not
-
-
?
additional information
?
-
AglB and AglA, an enzyme II(CB) component of the PEP:PTS, facilitate the phosphorylative translocation and subsequent cleavage of phosphorylated isomers of sucrose and related alpha-glycosides
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
dependent on NAD+, the glycosidic bond hydrolysis is initiated by oxidation of the glucopyranose ring at the 3-position to a keto group by the NAD+ molecule bound to the active site
additional information
requires a divalent metal ion, e.g. Mn2+
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Co2+
Fe2+
Mn2+
Ni2+
additional information
Mn2+
-
requirement, permits formation of the active tetramer of GlvA from its inactive dimeric state
additional information
requires a divalent metal ion, e.g. Mn2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
differential expression of MalH and PagL during growth on various sugars, enzyme induction, nucleotide specificity for activation of MalH and PagL
-
additional information
differential expression of MalH and PagL during growth on various sugars, enzyme induction, nucleotide specificity for activation of MalH and PagL
-
additional information
MalH is induced during growth on the sucrose isomers trehalulose, turanose, maltulose, leucrose and palatinose, but not on sucrose
-
additional information
-
growth on alpha-glycosides, such as maltose, alpha-methylglycoside, trehalose, turanose and palatinose, increases dramatically enzyme synthesis
-
additional information
growth on alpha-glycosides, such as maltose, alpha-methylglycoside, trehalose, turanose and palatinose, increases dramatically enzyme synthesis
-
additional information
-
growth on alpha-glycosides, such as maltose, alpha-methylglycoside, trehalose, turanose and palatinose, increases dramatically enzyme synthesis
-
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.004
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in Tris-HCl buffer, pH 8.4
0.012
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in HEPES buffer, pH 7.5
0.82
3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose
pH 7, 37°C
1.68
3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose
pH 7.5
0.014
4-nitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in Tris-HCl buffer, pH 8.4
0.052
4-nitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in HEPES buffer, pH 7.5
2.42
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose
pH 7.5
2.47
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose
pH 7, 37°C
0.5
methyl 6-phospho-alpha-D-glucoside
-
at 37°C in Tris-HCl buffer, pH 8.4
0.028
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate
pH 7, 37°C
0.058
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate
pH 7, 37°C
0.038
phenyl 6-phospho-alpha-D-glucoside
-
at 37°C in Tris-HCl buffer, pH 8.4
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.72
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in HEPES buffer, pH 7.5
0.81
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in Tris-HCl buffer, pH 8.4
0.7
4-nitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in HEPES buffer, pH 7.5
0.85
4-nitrophenyl 6-phospho-alpha-D-glucoside
-
at 37°C in Tris-HCl buffer, pH 8.4
1.1
methyl 6-phospho-alpha-D-glucoside
-
at 37°C in Tris-HCl buffer, pH 8.4
0.26
phenyl 6-phospho-alpha-D-glucoside
-
at 37°C in Tris-HCl buffer, pH 8.4
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Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0198
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on glucose
0.0203
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0225
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0246
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0259
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.027
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0276
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0289
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on maltose
0.0318
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.034
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0355
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0361
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0375
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0418
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0453
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0456
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0466
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0484
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0489
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0502
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0513
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0538
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0565
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.062
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.063
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0642
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0643
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.065
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0664
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.069
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.072
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0725
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.075
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.08
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0823
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.088
0.0997
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1019
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1045
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.1187
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.124
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.1363
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.1575
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1654
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
1.67
-
at room temperature, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
2.9
pH 7.5, 37°C, recombinant MalH, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
4.15
pH 7.5, recombinant AglB, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
4.9
pH 7.5, 37°C, recombinant MalH, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
additional information
0.088
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.088
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 7.5
-
maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
7.5
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
36
in 0.1 M Tris-HCl or HEPES buffer, pH 7.5, 0.1 mM NAD+, 1 mM Mn2+
37
40
-
maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
4.9
5.4
2 species with pI values of 5.4 and 5.6, analytical isoelectric focusing
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
strain ATCC 824, MalH, i.e. maltose 6-phosphate hydrolase
GenBank
brenda
strain ATCC 824, PagL, i.e. phospho-alpha-glucosidase
GenBank
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
Show AA Sequence and Transmembrane Helices (687 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
49000
49256
49972
4 * 49972, recombinant MalH, predicted from the amino acid sequence
50000
50038
4 * 50038, recombinant MalH, electrospray ionization mass spectrometry
51155
4 * 51155, recombinant PagL, predicted from the amino acid sequence
51205
4 * 51205, recombinant PagL, electrospray ionization mass spectrometry
50000
4 * 50000, recombinant MalH and PagL, SDS-PAGE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
dimer
tetramer
tetramer
4 * 51155, recombinant PagL, predicted from the amino acid sequence
tetramer
4 * 49972, recombinant MalH, predicted from the amino acid sequence
tetramer
4 * 50038, recombinant MalH, electrospray ionization mass spectrometry
tetramer
4 * 51205, recombinant PagL, electrospray ionization mass spectrometry
tetramer
4 * 50000, recombinant MalH and PagL, SDS-PAGE
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in the presence and absence of its known ligands, resolution at 2.05 A
recombinant GlvA expressed in Escherichia coli, vapour-phase diffusion using the hanging-drop method, NAD/Mn2+-dependent crystal form
-
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis and storage overnight results in 90% loss of activity
inclusion of 0.1 mM NAD+ and 1 mM Mn2+ in all buffers stabilizes during purification
inclusion of dithiothreitol and Mn2+ in chromatography buffers stabilizes during purification
-
dialysis and storage overnight results in 90% loss of activity
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
O2-sensitive enzyme, rapidly inactivated by exposure to air
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 25 mM HEPES buffer, pH 7.5, 1 mM Mn2+, 1 mM dithiothreitol, several weeks, stable
-
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
6fold, dithiothreitol and Mn2+ is required for stabilization during purification
-
recombinant MalH expressed in Escherichia coli PEP43
recombinant PagL expressed in Escherichia coli PEP43
recombinant MalH expressed in Escherichia coli PEP43
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
aglB gene is cloned, sequenced and expressed in Escherichia coli TOP 10
gene encoding maltose 6P hydrolase, a 441 residues protein, is cloned, sequenced and actively expressed in Escherichia coli
-
genes malh and pagl encoding maltose 6-phosphate hydrolase MalH and phospho-alpha-glucosidase PagL are cloned, sequenced and expressed in Escherichia coli PEP43, the 2 genes reside in separate operons, molecular organization of the operons mal and pagl
glvA gene, formerly glv1, is encoded within the maltose PEP-PTS operon, it is cloned and overexpressed in Escherichia coli, glvA encodes a 449 amino acid protein
-
malH gene encodes maltose 6P hydrolase, a 441 residues protein, and is cloned, sequenced and expressed in Escherichia coli XLOLR
plasmids encoding either aglB alone (pAP1) or both aglA and aglB genes (pAP2) expressed in Escherichia coli MG-1655
structural organization of the putative alpha-glycoside operon
structural organization of the putative alpha-glycoside operon, gene malH is adjacent to malB and malR, malH gene is cloned and expressed in Escherichia coli PEP43
structural organization of the putative alpha-glycoside operon
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wursch, P.; Koellreutter, B.
Maltotriitol inhibition of maltose metabolism in Streptococcus mutans via maltose transport, amylomaltase and phospho-alpha-glucosidase activities
Caries Res.
19
439-449
1985
Streptococcus mutans, Streptococcus mutans OMZ 176
brenda
Varrot, A.; Yamamoto, H.; Sekiguchi, J.; Thompson, J.; Davies, G.J.
Crystallization and preliminary X-ray analysis of the 6-phospho-alpha-glucosidase from Bacillus subtilis
Acta Crystallogr. Sect. D
55
1212-1214
1999
Bacillus subtilis, Bacillus subtilis GlvA
brenda
Thompson, J.; Gentry-Weeks, C.R.; Nguyen, N.Y.; Folk, J.E.; Robrish, S.A.
Purification from Fusobacterium mortiferum ATCC 25557 of a 6-phosphoryl-O-alpha-D-glucopyranosyl:6-phosphoglucohydrolase that hydrolyzes maltose 6-phosphate and related phospho-alpha-D-glucosides
J. Bacteriol.
177
2505-2512
1995
Fusobacterium mortiferum
brenda
Bouma, C.L.; Reizer, J.; Reizer, A.; Robrish, S.A.; Thompson, J.
6-Phospho-alpha-D-glucosidase from Fusobacterium mortiferum: Cloning, expression, and assignment to family 4 of the glycosylhydrolases
J. Bacteriol.
179
4129-4137
1997
Fusobacterium mortiferum (O06901), Fusobacterium mortiferum
brenda
Pikis, A.; Immel, S.; Robrish, S.A.; Thompson, J.
Metabolism of sucrose and its five isomers by Fusobacterium mortiferum
Microbiology
148
843-852
2002
Fusobacterium mortiferum (O06901), Escherichia coli (Q8XEI0), Klebsiella pneumoniae (Q9AGA6)
brenda
Thompson, J.; Robrish, S.A.; Immel, S.; Lichtenthaler, F.W.; Hall, B.G.; Pikis, A.
Metabolism of sucrose and its five linkage-isomeric alpha-D-glucosyl-D-fructoses by Klebsiella pneumoniae. Participation and properties of sucrose-6-phosphate hydrolase and phospho-alpha-glucosidase
J. Biol. Chem.
276
37415-37425
2001
Klebsiella pneumoniae (Q9AGA6)
brenda
Thompson, J.; Hess, S.; Pikis, A.
Genes malh and pagl of Clostridium acetobutylicum ATCC 824 encode NAD+- and Mn2+-dependent phospho-alpha-glucosidase(s)
J. Biol. Chem.
279
1553-1561
2004
Clostridium acetobutylicum (Q97DP6), Clostridium acetobutylicum (Q97LM4)
brenda
Rajan, S.S.; Yang, X.; Collart, F.; Yip, V.L.Y.; Withers, S.G.; Varrot, A.; Thompson, J.; Davies, G.J.; Anderson, W.F.
Novel catalytic mechanism of glycoside hydrolysis based on the structure of an NAD+/Mn2+-dependent phospho-alpha-glucosidase from Bacillus subtilis
Structure
12
1619-1629
2004
Bacillus subtilis (P54716)
brenda
Yip, V.L.; Thompson, J.; Withers, S.G.
Mechanism of GlvA from Bacillus subtilis: a detailed kinetic analysis of a 6-phospho-alpha-glucosidase from glycoside hydrolase family 4
Biochemistry
46
9840-9852
2007
Bacillus subtilis
brenda
Pikis, A.; Hess, S.; Arnold, I.; Erni, B.; Thompson, J.
Genetic requirements for growth of Escherichia coli K12 on methyl-alpha-D-glucopyranoside and the five alpha-D-glucosyl-D-fructose isomers of sucrose
J. Biol. Chem.
281
17900-17908
2006
Klebsiella pneumoniae (Q9AGA6)
brenda
Hall, B.G.; Pikis, A.; Thompson, J.
Evolution and biochemistry of family 4 glycosidases: implications for assigning enzyme function in sequence annotations
Mol. Biol. Evol.
26
2487-2497
2009
Symbiobacterium thermophilum IAM 14863
brenda
Huang, W.; Llano, J.; Gauld, J.W.
Redox mechanism of glycosidic bond hydrolysis catalyzed by 6-phospho-alpha-glucosidase: a DFT study
J. Phys. Chem. B
114
11196-11206
2010
Bacillus subtilis (P54716)
brenda
Mokhtari, A.; Blancato, V.S.; Repizo, G.D.; Henry, C.; Pikis, A.; Bourand, A.; de Fatima Alvarez, M.; Immel, S.; Mechakra-Maza, A.; Hartke, A.; Thompson, J.; Magni, C.
Deutscher, J.: Enterococcus faecalis utilizes maltose by connecting two incompatible metabolic routes via a novel maltose 6'-phosphate phosphatase (MapP)
Mol. Microbiol.
88
234-253
2013
Enterococcus faecalis, Enterococcus faecalis JH2-2
brenda
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