Information on EC 3.2.1.122 - maltose-6'-phosphate glucosidase

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The expected taxonomic range for this enzyme is: Bacteria

EC NUMBER
COMMENTARY hide
3.2.1.122
-
RECOMMENDED NAME
GeneOntology No.
maltose-6'-phosphate glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
alpha-maltose 6'-phosphate + H2O = D-glucose + D-glucose 6-phosphate
show the reaction diagram
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-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Starch and sucrose metabolism
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SYSTEMATIC NAME
IUBMB Comments
maltose-6'-phosphate 6-phosphoglucohydrolase
Hydrolyses a variety of 6-phospho-alpha-D-glucosides, including alpha-maltose 6'-phosphate, alpha,alpha-trehalose 6-phosphate, sucrose 6-phosphate and p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate (as a chromogenic substrate). The enzyme is activated by FeII, MnII, CoII and NiII. It is rapidly inactivated in air.
CAS REGISTRY NUMBER
COMMENTARY hide
98445-08-0
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GlvA
-
-
Manually annotated by BRENDA team
enterohaemorrhagic strain O157:H7, GlvG
GenBank
Manually annotated by BRENDA team
strain OMZ 176
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Manually annotated by BRENDA team
strain OMZ 176
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
1-methyl-alpha-glucopyranoside + H2O
?
show the reaction diagram
-
-
-
-
?
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside + H2O
3,4-dinitrophenol + alpha-D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
4-methylumbelliferyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + 4-methylumbelliferone
show the reaction diagram
4-nitrophenyl 6-phospho-alpha-D-glucoside + H2O
4-nitrophenol + alpha-D-glucose
show the reaction diagram
-
-
-
-
?
4-nitrophenyl 6-phospho-beta-D-glucoside + H2O
4-nitrophenol + beta-D-glucose 6-phosphate
show the reaction diagram
-
-
-
-
?
6-phospho-beta-D-glucose + H2O
phosphate + D-glucose
show the reaction diagram
-
substrate analog
-
-
?
esculin 6-phosphate + H2O
?
show the reaction diagram
isomaltose 6'-phosphate + H2O
alpha-D-glucopyranosyl 6-phosphate + D-glucose
show the reaction diagram
-
-
-
-
?
leucrose 6'-phosphate + H2O
?
show the reaction diagram
-
-
-
?
leucrose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
maltitol 6-phosphate + H2O
alpha-D-glucopyranosyl 6-phosphate + D-glucitol
show the reaction diagram
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-
-
-
?
maltose 6'-phosphate + H2O
?
show the reaction diagram
-
-
-
?
maltose 6'-phosphate + H2O
D-glucose + D-glucose 6-phosphate
show the reaction diagram
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
maltose 6,6'-bisphosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
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6.0% activity compared to maltose 6'-phosphate
-
-
?
maltulose 6'-phosphate + H2O
?
show the reaction diagram
-
-
-
?
maltulose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
methyl 6-phospho-alpha-D-glucoside + H2O
methanol + alpha-D-glucose 6-phosphate
show the reaction diagram
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-
-
-
?
methyl-alpha-D-glucopyranoside + H2O
methanol + alpha-D-glucose
show the reaction diagram
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-
-
?
p-nitrophenyl alpha-D-glucopyranoside 6-phosphate + H2O
p-nitrophenol + alpha-D-glucose 6-phosphate
show the reaction diagram
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-
-
?
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate + H2O
alpha-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
beta-D-glucose 6-phosphate + p-nitrophenol
show the reaction diagram
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-
-
?
palatinose 6'-phosphate + H2O
?
show the reaction diagram
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-
-
?
palatinose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
phenyl 6-phospho-alpha-D-glucoside + H2O
phenol + alpha-D-glucose 6-phosphate
show the reaction diagram
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-
-
-
?
sucrose 6-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
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hydrolysis to yield D-glucose 6-phosphate and aglycone moiety in 1:1 molar ratio
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-
?
trehalose 6-phosphate + H2O
?
show the reaction diagram
trehalulose 6'-phosphate + H2O
?
show the reaction diagram
-
-
-
?
trehalulose 6'-phosphate + H2O
alpha-D-glucose 6-phosphate + D-fructose
show the reaction diagram
turanose 6'-phosphate + H2O
?
show the reaction diagram
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-
-
?
turanose 6'-phosphate + H2O
D-glucose 6-phosphate + D-fructose
show the reaction diagram
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
maltose 6'-phosphate + H2O
D-glucose + D-glucose 6-phosphate
show the reaction diagram
maltose 6'-phosphate + H2O
D-glucose 6-phosphate + D-glucose
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
-
requirement
additional information
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
air
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exposure to air rapidly inactivates
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cellobiose 6'-phosphate
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competitive inhibitor
maltotriitol
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strong
methyl 6-phospho-alpha-D-glucoside
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competitive inhibitor
sulfhydryl-reactive agent
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rapidly inactivates
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
dithiothreitol
dithiothreitol-dependent; dithiothreitol-dependent
additional information
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004 - 0.012
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
0.82 - 1.68
3-O-(6-O-phosphono-alpha-D-glucopyranosyl)-alpha-D-fructofuranose
0.014 - 0.052
4-nitrophenyl 6-phospho-alpha-D-glucoside
2.42 - 2.47
6-O-(6-O-phosphono-alpha-D-glucopyranosyl)-beta-D-fructofuranose
4.48
isomaltose 6'-phosphate
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pH 7.5
1.87 - 5.63
leucrose 6'-phosphate
0.82
maltitol 6-phosphate
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pH 7.5
0.36 - 4.1
maltose 6'-phosphate
1.11 - 1.2
maltulose 6'-phosphate
0.5 - 0.61
methyl 6-phospho-alpha-D-glucoside
0.028 - 2
p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate
0.038 - 0.069
phenyl 6-phospho-alpha-D-glucoside
1.16
Trehalose 6-phosphate
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pH 7.5
1.23 - 1.92
trehalulose 6'-phosphate
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.72 - 0.81
3,4-dinitrophenyl 6-phospho-alpha-D-glucoside
0.7 - 0.85
4-nitrophenyl 6-phospho-alpha-D-glucoside
1.3 - 128
maltose 6'-phosphate
0.42 - 1.1
methyl 6-phospho-alpha-D-glucoside
0.26 - 0.9
phenyl 6-phospho-alpha-D-glucoside
Ki VALUE [mM]
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.03
cellobiose 6'-phosphate
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-
0.075
methyl 6-phospho-alpha-D-glucoside
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0198
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on glucose
0.0203
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0225
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0246
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0259
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.027
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0276
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0289
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on maltose
0.0318
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.034
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0355
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0361
with methyl-alpha-D-glucopyranoside as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0375
with leucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0418
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0453
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0456
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0466
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0484
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0489
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0502
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0513
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0538
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.0565
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.062
with palatinose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.063
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0642
with p-nitrophenyl alpha-D-glucopyranoside 6-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0643
with maltose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.065
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.0664
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.069
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.072
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.0725
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.075
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.08
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.0823
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.088
with sucrose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose; with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.0997
with trehalulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1019
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1045
with maltulose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
0.1187
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on methyl-alpha-D-glucopyranoside
0.124
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on glucose
0.1363
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on palatinose
0.1575
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on maltose
0.1654
with turanose 6'-phosphate as substrate and MG-1655 (pAP2) grown previously on leucrose
1.67
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at room temperature, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
2.9
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pH 7.5, 37C, recombinant MalH, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
4.15
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pH 7.5, recombinant AglB, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
4.9
pH 7.5, 37C, recombinant MalH, p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
additional information
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7 - 7.5
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maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
36
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in 0.1 M Tris-HCl or HEPES buffer, pH 7.5, 0.1 mM NAD+, 1 mM Mn2+
40
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maximum rate of p-nitrophenyl-alpha-D-glucopyranoside 6-phosphate hydrolysis
pI VALUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.9
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analytical isoelectric focusing
5.4
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2 species with pI values of 5.4 and 5.6, analytical isoelectric focusing
5.5
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amino acid sequence
5.69
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theoretical pI deduced from the amino acid composition
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
PDB
SCOP
CATH
ORGANISM
UNIPROT
Bacillus subtilis (strain 168)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
100000
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about, gel filtration
200000
about, MalH and PagL, HPLC gel filtration; about, MalH and PagL, HPLC gel filtration
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
tetramer
Crystallization/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
in the presence and absence of its known ligands, resolution at 2.05 A
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recombinant GlvA expressed in Escherichia coli, vapour-phase diffusion using the hanging-drop method, NAD/Mn2+-dependent crystal form
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dialysis and storage overnight results in 90% loss of activity
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freezing and thawing from -20C retains 50% of initial activity
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inclusion of 0.1 mM NAD+ and 1 mM Mn2+ in all buffers stabilizes during purification
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inclusion of dithiothreitol and Mn2+ in chromatography buffers stabilizes during purification
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purification by Rotofor isoelectric focusing inactivates
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unstable enzyme
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OXIDATION STABILITY
ORGANISM
UNIPROT
LITERATURE
O2-sensitive enzyme, rapidly inactivated by exposure to air
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646682, 646683
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 25 mM HEPES buffer, pH 7.5, 1 mM Mn2+, 1 mM dithiothreitol, several weeks, stable
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storage overnight, 90% loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
6fold, dithiothreitol and Mn2+ is required for stabilization during purification
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recombinant AglB expressed in Escherichia coli TOP 10, 3.4fold
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recombinant enzyme
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recombinant GlvA expressed in Escherichia coli
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recombinant MalH expressed in Escherichia coli PEP43
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recombinant MalH expressed in Escherichia coli PEP43; recombinant PagL expressed in Escherichia coli PEP43
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
aglB gene is cloned, sequenced and expressed in Escherichia coli TOP 10; structural organization of the putative alpha-glycoside operon
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expression in Escherichia coli
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gene encoding maltose 6P hydrolase, a 441 residues protein, is cloned, sequenced and actively expressed in Escherichia coli
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genes malh and pagl encoding maltose 6-phosphate hydrolase MalH and phospho-alpha-glucosidase PagL are cloned, sequenced and expressed in Escherichia coli PEP43, the 2 genes reside in separate operons, molecular organization of the operons mal and pagl; genes malh and pagl encoding maltose 6-phosphate hydrolase MalH and phospho-alpha-glucosidase PagL are cloned, sequenced and expressed in Escherichia coli PEP43, the 2 genes reside in separate operons, molecular organization of the operons mal and pagl
glvA gene, formerly glv1, is encoded within the maltose PEP-PTS operon, it is cloned and overexpressed in Escherichia coli, glvA encodes a 449 amino acid protein
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malH gene encodes maltose 6P hydrolase, a 441 residues protein, and is cloned, sequenced and expressed in Escherichia coli XLOLR
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plasmids encoding either aglB alone (pAP1) or both aglA and aglB genes (pAP2) expressed in Escherichia coli MG-1655
structural organization of the putative alpha-glycoside operon
structural organization of the putative alpha-glycoside operon, gene malH is adjacent to malB and malR, malH gene is cloned and expressed in Escherichia coli PEP43
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ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C169S
catalytically inactive MalH mutant
D170N
catalytically inactive MalH mutant
M171V
MalH mutant with reduced activity
P172A
MalH mutant with reduced activity
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