Information on EC 3.2.1.121 - polymannuronate hydrolase

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The expected taxonomic range for this enzyme is: Pseudomonas aeruginosa

EC NUMBER
COMMENTARY hide
3.2.1.121
-
RECOMMENDED NAME
GeneOntology No.
polymannuronate hydrolase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
endohydrolysis of the D-mannuronide linkages of polymannuronate
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
poly(mannuronide) mannuronohydrolase
Does not act on alginic acid, which is a copolymer of polymannuronate.
CAS REGISTRY NUMBER
COMMENTARY hide
99283-64-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alginic acid + H2O
?
show the reaction diagram
-
little activity
-
-
?
polymannuronic acid exopolysaccharide + H2O
?
show the reaction diagram
-
from Pseudomonas aeruginosa, strain PM1
-
-
?
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
polymannuronic acid exopolysaccharide + H2O
?
show the reaction diagram
-
from Pseudomonas aeruginosa, strain PM1
-
-
?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CaCl2
-
5 mM: slight stimulation, at 17 mM inhibition
KCl
-
3 mM: stimulation, above 10 mM: inhibition
MgCl2
-
3 mM: slight stimulation, at 9 mM inhibition
MgSO4
-
3 mM: slight stimulation, at 14 mM inhibition
NaCl
-
3 mM: stimulation, above 10 mM: inhibition
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
CaCl2
-
at 17 mM inhibition
EDTA
-
0.5 mM: 35% inhibition
KCl
-
50 mM: 50% inhibition
MgCl2
-
at 9 mM inhibition
MgSO4
-
at 14 mM inhibition
NaCl
-
50 mM: 40% inhibition
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.096
polymannuronic acid exopolysaccharide
-
37°C, pH 7.5, from Pseudomonas aeruginosa, strain PM1
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.8
-
sonicated cells with EDTA
2
-
sonicated cells without EDTA
3.8
-
supernatant of cells with EDTA
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
associated with outer cell membrane
Manually annotated by BRENDA team
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
major band: 53000 Da, minor band: 23000 Da, SDS-PAGE
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE