Information on EC 3.2.1.116 - glucan 1,4-alpha-maltotriohydrolase

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The expected taxonomic range for this enzyme is: Bacteria, Archaea

EC NUMBER
COMMENTARY
3.2.1.116
-
RECOMMENDED NAME
GeneOntology No.
glucan 1,4-alpha-maltotriohydrolase
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
Hydrolysis of (1->4)-alpha-D-glucosidic linkages in amylaceous polysaccharides, to remove successive maltotriose residues from the non-reducing chain ends
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
4-alpha-D-glucan maltotriohydrolase
Cf. EC 3.2.1.2 (beta-amylase), EC 3.2.1.60 (glucan 1,4-alpha-maltotetraohydrolase) and EC 3.2.1.98 (glucan 1,4-alpha-maltohexaosidase). The products have the alpha-configuration.
SYNONYMS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
exo-maltotriohydrolase
-
-
-
-
maltotriohydrolase
-
-
-
-
maltotriohydrolase, exo-
-
-
-
-
maltotriose-producing alpha-amylase
-
-
maltotriose-producing alpha-amylase
Thermobifida fusca NTU22
-
-
-
CAS REGISTRY NUMBER
COMMENTARY
91273-84-6
-
ORGANISM
COMMENTARY
LITERATURE
SEQUENCE CODE
SEQUENCE DB
SOURCE
strain Ah-36
-
-
Manually annotated by BRENDA team
strain Ah-36
-
-
Manually annotated by BRENDA team
strain NTU22
-
-
Manually annotated by BRENDA team
Thermobifida fusca NTU22
strain NTU22
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                      
REACTION DIAGRAM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3 glycogen + H2O
4 maltotriose
show the reaction diagram
-
-
main product
?
3 glycogen + H2O
4 maltotriose
show the reaction diagram
-
from oyster, slow hydrolysis
additional small amounts of maltose and glucose, alpha-configuration
?
amylopectin + H2O
maltotriose
show the reaction diagram
-
-
main product
?
amylopectin + H2O
maltotriose
show the reaction diagram
-
-
additional small amounts of maltose and glucose, alpha-configuration
?
amylose + H2O
maltotriose
show the reaction diagram
-
-
-
-
-
amylose + H2O
maltotriose
show the reaction diagram
-
-
main product
?
amylose + H2O
maltotriose
show the reaction diagram
-
partially oxidized potato amylose
-
?
beta-cyclodextrin + H2O
maltotriose
show the reaction diagram
-
-
main product
?
gamma-cyclodextrin + H2O
?
show the reaction diagram
-
-
-
-
?
maltoheptaose + H2O
maltotriose
show the reaction diagram
-
-
main product, additional small amounts of maltose and glucose, alpha-configuration
?
maltohexaose + H2O
maltotriose
show the reaction diagram
-
-
alpha-configuration
?
maltohexaose + H2O
maltotriose
show the reaction diagram
-
-
alpha-configuration, main product, additional small amounts of maltose and glucose
?
maltohexaose + H2O
maltotriose
show the reaction diagram
-
-
alpha-configuration, main product, additional small amounts of maltose and glucose
?
maltooligosaccharide + H2O
maltotriose
show the reaction diagram
-
hydrolysis of maltohexaose, maltopentaose, maltotetraose and maltotriose
alpha-configuration
?
maltooligosaccharide + H2O
maltotriose
show the reaction diagram
-
ranging from maltotetraose to maltoheptaose, not: maltotriose, maltose
alpha-configuration
?
maltooligosaccharide + H2O
maltotriose
show the reaction diagram
-
enzyme hydrolyzes maltooligosaccharides larger than maltotriose by an exo-mechanism from the nonreducing end
alpha-configuration
?
maltopentaose + H2O
maltotriose + D-maltose
show the reaction diagram
-
-
alpha-configuration
?
maltopentaose + H2O
maltotriose + D-maltose
show the reaction diagram
-
-
alpha-configuration, additional small amounts of glucose
?
maltotetraose + H2O
maltotriose + D-glucose
show the reaction diagram
-
-
alpha-configuration, additional product: D-maltose
?
maltotetraose + H2O
maltotriose + D-glucose
show the reaction diagram
-
-
alpha-configuration, additional product: D-maltose
?
raw sago starch + H2O
maltotriose + ?
show the reaction diagram
Thermobifida fusca, Thermobifida fusca NTU22
-
-
-
-
?
short-chain amylose + H2O
maltotriose
show the reaction diagram
-
-
alpha-configuration, major initial product, prolonged action leads to gradual hydrolysis of maltotriose to glucose and maltose
?
short-chain amylose + H2O
maltotriose
show the reaction diagram
-
DP, 17, most rapid hydrolysis
alpha-configuration, additional small amounts of maltose and glucose
?
soluble starch + H2O
maltotriose + ?
show the reaction diagram
Thermobifida fusca, Thermobifida fusca NTU22
-
-
-
-
?
starch + H2O
maltotriose
show the reaction diagram
-
soluble
additional small amounts of maltose and glucose, alpha-configuration
?
starch + H2O
maltotriose
show the reaction diagram
-
soluble
initial main product, proceeded reaction results in 55-60% maltotriose, 10-20% maltose, 1-3% glucose, 20-40% higher oligosaccharides
?
maltotriose + H2O
maltose + D-glucose
show the reaction diagram
-
slow hydrolysis
alpha-configuration
?
additional information
?
-
-
not: alpha-cyclodextrin, pullulan
-
-
-
additional information
?
-
-
not: alpha-cyclodextrin, pullulan
-
-
-
additional information
?
-
-
no hydrolysis of alpha-1,6-glucosidic linkages
-
-
-
additional information
?
-
-
not: maltotriose, maltose, beta-cyclodextrin, enzyme has transferase activity using maltopentaose or maltotetraose as substrates
-
-
-
additional information
?
-
-
not: alpha-cyclodextrin, pullulan, not: maltotriose, maltose, beta-cyclodextrin, enzyme has transferase activity using maltopentaose or maltotetraose as substrates
-
-
-
METALS and IONS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
CsCl
-
can partially substitute for NaCl at higher concentrations
KCl
-
can partially substitute for NaCl at higher concentrations
NaCl
-
requirement, maximum activity at 2.5 M NaCl, no activity below 1 M NaCl
RbCl
-
can partially substitute for NaCl at higher concentrations
INHIBITORS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
alpha-cyclodextrin
-
competitive inhibitor, 0.25%
beta-cyclodextrin
-
competitive inhibitor, 0.025%
Ca2+
-
1 mM, 33% inhibition
Hg2+
-
weak, 1 mM: 15% inhibition, 10 mM: 75% inhibition
N-bromosuccinimide
-
1 mM, 73% inhibition
Ni2+
-
1 mM, 33% inhibition
SDS
-
0.1%, 80% inhibition
Urea
-
1 M, 74% inhibition
ZnCl2
-
1 mM, complete inhibition
additional information
-
not inhibited by 1 mM iodoacetic acid, 1 mM phenylmethylsulfonyl fluoride
-
KM VALUE [mM]
KM VALUE [mM] Maximum
SUBSTRATE
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
49
-
Amylopectin
-
pH 9, 50C, Km-value shown as glucose equivalents
19
-
Amylose
-
pH 9, 50C, DP, 17, Km-value shown as glucose equivalents
6.8
-
gamma-cyclodextrin
-
pH 9, 50C, Km-value shown as glucose equivalents
25
-
starch
-
pH 9, 50C, soluble, Km-value shown as glucose equivalents
42
-
glycogen
-
pH 9, 50C, from oyster, Km-value shown as glucose equivalents
additional information
-
additional information
-
Km-value for soluble starch is 0.88 mg/ml
-
Ki VALUE [mM]
Ki VALUE [mM] Maximum
INHIBITOR
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
IMAGE
1.63
-
alpha-cyclodextrin
-
pH 9, 50C, 0.25%
0.41
-
beta-cyclodextrin
-
pH 9, 50C, 0.025%
SPECIFIC ACTIVITY [µmol/min/mg]
SPECIFIC ACTIVITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
160
-
-
pH 9, 50C
additional information
-
-
-
pH OPTIMUM
pH MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
7
-
-
assay at
8.7
-
-
in the presence of 2.5 M NaCl
9
-
-
assay at
pH RANGE
pH RANGE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
5.8
8.5
-
pH 5.8: about 90% of activity maximum, pH 8.5: about 30% of activity maximum
TEMPERATURE OPTIMUM
TEMPERATURE OPTIMUM MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
-
-
assay at
50
-
-
assay at
55
-
-
pH 9, 30 min, in the presence of 2.5 M NaCl
TEMPERATURE RANGE
TEMPERATURE MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
25
55
-
about 50% of activity maximum at 25C and 55C
LOCALIZATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
MOLECULAR WEIGHT MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40000
-
-
gel filtration
60000
-
-
gel filtration
76000
-
-
gel filtration
SUBUNITS
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
?
-
x * 25500, SDS-PAGE
monomer
-
1 * 74000, SDS-PAGE
monomer
-
1 * 64000, SDS-PAGE
monomer
-
1 * 74000, SDS-PAGE
-
monomer
Thermobifida fusca NTU22
-
1 * 64000, SDS-PAGE
-
pH STABILITY
pH STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
3.5
-
-
30 min, 50C, 2.5 M NaCl, 0.5% soluble starch, 80% loss of activity
4.2
-
-
30 min, 50C, 2.5 M NaCl, 80% loss of activity
5
9
-
stable in the pH range
5
-
-
30 min, 50C, 2.5 M NaCl, 44% loss of activity
6
10
-
30 min, 50C, 2.5 M NaCl, 0.5% soluble starch, stable
6
8.6
-
30 min, 50C, 2.5 M NaCl, stable
10.5
-
-
30 min, 50C, 2.5 M NaCl, 30% loss of activity
TEMPERATURE STABILITY
TEMPERATURE STABILITY MAXIMUM
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
40
-
-
unstable above
50
-
-
30 min, 2.5 M NaCl, pH 4.2: 80% loss of activity, pH 5.0: 44% loss of activity, pH 6.0-8.6: stable, pH 10.5: 30% loss of activity
55
-
-
pH 8.0, 30 min, 2.5 M NaCl, 50% loss of activity, no loss of activity in the presence of 0.5% soluble starch
60
-
-
3 h, about 70% of the activity remains
additional information
-
-
Ca2+ protects against heat inactivation
additional information
-
-
soluble starch stabilizes against thermal inactivation, Ca2+ does not enhance thermal stability
GENERAL STABILITY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
0.5% soluble starch stabilizes
-
2.5 M NaCl stabilizes
-
activity is irreversibly lost at low ionic strength
-
no loss of activity after dialysis
-
Purification/COMMENTARY
ORGANISM
UNIPROT ACCESSION NO.
LITERATURE
2000fold, in the presence of 2.5 M NaCl
-
APPLICATION
ORGANISM
UNIPROT ACCESSION NO.
COMMENTARY
LITERATURE
nutrition
-
enzyme produced on the industrial scale for the production of high maltotriose-containing syrup