Information on EC 3.2.1.112 - 2-deoxyglucosidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.112
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RECOMMENDED NAME
GeneOntology No.
2-deoxyglucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
a 2-deoxy-alpha-D-glucoside + H2O = 2-deoxy-D-glucose + a primary alcohol
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
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SYSTEMATIC NAME
IUBMB Comments
2-deoxy-alpha-D-glucoside deoxyglucohydrolase
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CAS REGISTRY NUMBER
COMMENTARY hide
92480-05-2
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
P288 leukemia cells
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Manually annotated by BRENDA team
HTC cells and liver acetone powder
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
H2O + a 2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + an alcohol
show the reaction diagram
H2O + p-nitrophenyl-2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + p-nitrophenol
show the reaction diagram
H2O + p-nitrophenyl-alpha-D-glucoside
alpha-D-glucose + p-nitrophenol
show the reaction diagram
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poor substrate
-
?
H2O + p-nitrophenyl-alpha-D-mannoside
alpha-D-mannose + p-nitrophenol
show the reaction diagram
H2O + p-nitrophenyl-beta-D-glucoside
beta-D-glucose + p-nitrophenol
show the reaction diagram
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poor substrate
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?
H2O + p-nitrophenyl-beta-N-acetyl-D-glucosaminoside
beta-N-acetyl-D-glucosamine + p-nitrophenol
show the reaction diagram
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
H2O + a 2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + an alcohol
show the reaction diagram
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-Mannono-1,5-lactone
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2
p-nitrophenyl-2-deoxy-alpha-D-glucoside
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pH 7, 37C
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.035
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partially purified enzyme, pH 7, 37C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 8.5
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much lower activity with Tris than with HCl/KCl buffer
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4 - 10
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much lower activity with Tris than with HCl/KCl buffer
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
50
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stable for at least 5 min
60
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98% loss of activity within 5 min
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
all stages of purification can be stored at -20C for 18 months with no loss of activity
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20C, 18 months, no loss of activity
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
partial
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
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potential target to increase effectiveness of 2-deoxy-D-glucose in chemotherapy and viral infections