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The enzyme appears in viruses and cellular organisms
Synonyms
2-deoxy-alpha-D-glucosidase, 2-deoxy-alpha-glucosidase,
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2-deoxy-alpha-D-glucosidase
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2-deoxy-alpha-glucosidase
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a 2-deoxy-alpha-D-glucoside + H2O = 2-deoxy-D-glucose + a primary alcohol
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hydrolysis of O-glycosyl bond
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2-deoxy-alpha-D-glucoside deoxyglucohydrolase
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H2O + a 2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + an alcohol
H2O + p-nitrophenyl-2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + p-nitrophenol
H2O + p-nitrophenyl-alpha-D-glucoside
alpha-D-glucose + p-nitrophenol
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poor substrate
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H2O + p-nitrophenyl-alpha-D-mannoside
alpha-D-mannose + p-nitrophenol
H2O + p-nitrophenyl-beta-D-glucoside
beta-D-glucose + p-nitrophenol
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poor substrate
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H2O + p-nitrophenyl-beta-N-acetyl-D-glucosaminoside
beta-N-acetyl-D-glucosamine + p-nitrophenol
H2O + a 2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + an alcohol
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H2O + a 2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + an alcohol
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physiological function is unknown, might contribute to regulation of glucose metabolism and synthesis of glycosylated macromolecules
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H2O + a 2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + an alcohol
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H2O + a 2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + an alcohol
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physiological function is unknown, might contribute to regulation of glucose metabolism and synthesis of glycosylated macromolecules
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H2O + p-nitrophenyl-2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + p-nitrophenol
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H2O + p-nitrophenyl-2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + p-nitrophenol
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H2O + p-nitrophenyl-alpha-D-mannoside
alpha-D-mannose + p-nitrophenol
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poor substrate
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H2O + p-nitrophenyl-alpha-D-mannoside
alpha-D-mannose + p-nitrophenol
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poor substrate
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H2O + p-nitrophenyl-beta-N-acetyl-D-glucosaminoside
beta-N-acetyl-D-glucosamine + p-nitrophenol
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H2O + p-nitrophenyl-beta-N-acetyl-D-glucosaminoside
beta-N-acetyl-D-glucosamine + p-nitrophenol
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H2O + a 2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + an alcohol
H2O + a 2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + an alcohol
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physiological function is unknown, might contribute to regulation of glucose metabolism and synthesis of glycosylated macromolecules
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r
H2O + a 2-deoxy-alpha-D-glucoside
2-deoxy-D-glucose + an alcohol
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physiological function is unknown, might contribute to regulation of glucose metabolism and synthesis of glycosylated macromolecules
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r
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D-mannono-1,5-lactone
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D-mannono-1,5-lactone
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2
p-nitrophenyl-2-deoxy-alpha-D-glucoside
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pH 7, 37°C
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0.035
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partially purified enzyme, pH 7, 37°C
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6.5 - 8.5
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much lower activity with Tris than with HCl/KCl buffer
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4 - 10
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much lower activity with Tris than with HCl/KCl buffer
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P288 leukemia cells
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HTC cells and liver acetone powder
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50
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stable for at least 5 min
60
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98% loss of activity within 5 min
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all stages of purification can be stored at -20°C for 18 months with no loss of activity
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-20°C, 18 months, no loss of activity
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partial
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medicine
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potential target to increase effectiveness of 2-deoxy-D-glucose in chemotherapy and viral infections
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Canellakis, Z.N.; Bondy, P.K.; May, Jr., J.A.; Myers-Robfogel, M.K.; Sartorelli, A.C.
Indentification of a glycosidase activity with apparent specificity for 2-deoxy-D-glucose in glycosidic linkage
Eur. J. Biochem.
143
159-163
1984
Mus musculus, Rattus norvegicus
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