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Information on EC 3.2.1.11 - dextranase and Organism(s) Talaromyces minioluteus and UniProt Accession P48845
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3.2.1.11 dextranaseSpecify your search results
Word Map
- 3.2.1.11
- dextrans
- streptococcus
-
dental
- glucans
- plaque
- penicillium
- caries
- leuconostoc
- sobrinus
- dextransucrase
- isomaltose
-
water-insoluble
-
cariogenic
- mesenteroides
-
colon-specific
- lipomyces
- downei
- sanguis
-
glucosyltransferases
- funiculosum
- salivarius
- mutanase
- synthesis
- medicine
- starkeyi
- isomaltotriose
- agriculture
- degradation
- nutrition
- drug development
- isomalto-oligosaccharides
- biotechnology
- food industry
-
glucan-binding
The taxonomic range for the selected organisms is: Talaromyces minioluteus
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
dextranase, endodextranase, endo-dextranase, extracellular dextranase, aodex, alpha-glucanase, dex410, tpdex, smdex, dex49a, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Alpha-1,6-glucan-6-glucanohydrolase
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alpha-D-1,6-glucan-6-glucanohydrolase
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-
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dextran hydrolase
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-
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dextranase DL 2
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DL 2
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-
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endo-dextranase
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endodextranase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
hydrolysis of O-glycosyl bond
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-
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
6-alpha-D-glucan 6-glucanohydrolase
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CAS REGISTRY NUMBER
COMMENTARY
9025-70-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-sindacene-3-propionic acid, succinimidyl ester)-quenched dextran + H2O
?
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
aflatoxin B1
inhibits dextranase activities of CYP3A4-expressing cultures, maximum inhibition of 22% at 2 microg/ml. Only negligibly inhibits strain INVSC1 expressing dextranase
aflatoxin G1
substantially less inhibitory towards dextranase activities of CYP3A4-expressing cultures than aflatoxin B1, showing a maximum inhibition of about 12% at 2 microg/ml, whereas strain INVSC1 expressing dextranase is only marginally inhibited
T-2 toxin
100% inhibition at 10 microg/ml of dextranase activities of CYP3A4-expressing cultures and strain INVSC1
additional information
no significant inhibition of dextranase activities of CYP3A4-expressing cultures and strain INVSC1 at concentrations of 2, 20, 200, and 2000 ng/ml by deoxynivalenol, fumonisin B1, and ochratoxin A
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additional information
-
no significant inhibition of dextranase activities of CYP3A4-expressing cultures and strain INVSC1 at concentrations of 2, 20, 200, and 2000 ng/ml by deoxynivalenol, fumonisin B1, and ochratoxin A
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
3 - 6
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approx. 50% of maximal activity at pH 6.3, complete loss of activity above pH 7.5
pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). DEXT_TALMI
608
0
65961
Swiss-Prot
Secretory Pathway (Reliability: 1)
PDB
SCOP
CATH
UNIPROT
ORGANISM
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
POSTTRANSLATIONAL MODIFICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
glycoprotein
glycoprotein
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10-12% of dextranase mass corresponds to carbohydrate in the form of N-linked oligosaccharide chains
glycoprotein
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3 potential N-glycosylation sites, differences in glycosylation profiles between native and recombinant dextranase
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of apo-Dex49A and Dex49A complexed with product at 1.8 A and 1.65 A, respectively
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae INVSc1 via fusion of the DNA segment encoding the mature dextranase protein with alpha-factor signal sequence, and insertion into the GAL1-controlled expression vector pYES2/CT (construction of the four dextranase-expressing plasmids pYES2/CT-dex, pYES2/CT-dex-tag, pYES2/CT-alphaF-dex(m) and pYES2/CT-alphaF-dex(m)-tag). Dextranase construct pYESCT2-alphaF-dex(m) introduced into a strain of Saccharomyces cerevisiae expressing the human cytochrome P4503A4 (CYP3A4) and the cognate reductase
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Beldarrain, A.; Acosta, N.; Betancourt, L.; Gonzalez, L.J.; Pons, T.
Enzymic, spectroscopic and calorimetric studies of a recombinant dextranase expressed in Pichia pastoris
Biotechnol. Appl. Biochem.
38
211-221
2003
Talaromyces minioluteus
Betancourt, L.H.; Garcia, R.; Gonzalez, J.; Montesino, R.; Quintero, O.; Takao, T.; Shimonishi, Y.; Cremata, J.A.
Dextranase (alpha-1,6 glucan-6-glucanohydrolase) from Penicillium minioluteum expressed in Pichia pastoris: two host cells with minor differences in N-glycosylation
FEMS Yeast Res.
1
151-160
2001
Talaromyces minioluteus
Larsson, A.M.; Andersson, R.; Stahlberg, J.; Kenne, L.; Jones, T.A.
Dextranase from Penicillium minioluteum: reaction course, crystal structure, and product complex
Structure
11
1111-1121
2003
Talaromyces minioluteus (P48845), Talaromyces minioluteus
Li, X.; Millson, S.H.; Coker, R.D.; Evans, I.H.
Cloning and expression of Penicillium minioluteum dextranase in Saccharomyces cerevisiae and its exploitation as a reporter in the detection of mycotoxins
Biotechnol. Lett.
28
1955-1964
2006
Talaromyces minioluteus (Q27JJ3), Talaromyces minioluteus, Talaromyces minioluteus IMI068219 (Q27JJ3)
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