Information on EC 3.2.1.109 - endogalactosaminidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.109
-
RECOMMENDED NAME
GeneOntology No.
endogalactosaminidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
Endohydrolysis of (1->4)-alpha-D-galactosaminidic linkages in poly(D-galactosamine)
show the reaction diagram
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
galactosaminoglycan glycanohydrolase
-
CAS REGISTRY NUMBER
COMMENTARY hide
59088-26-5
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
881
-
-
Manually annotated by BRENDA team
881
-
-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
oligogalactosaminoglycan + H2O
?
show the reaction diagram
-
endohydrolysis of 1,4-alpha-galactosaminidic linkages
-
-
?
polygalactosaminoglycan + H2O
?
show the reaction diagram
-
high molecular weight substrate obtained from Neurospora
-
-
?
polygalactosaminoglycan + H2O
galactosamine + GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN + GalNalpha1-4GalNalpha1-4GalNalpha1-4GalNalpha1-4GalN
show the reaction diagram
additional information
?
-
-
sporeling detaching activity, not: N-acetyl-oligogalactosaminoglycan and chitosan
-
-
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
polygalactosaminoglycan + H2O
?
show the reaction diagram
-
high molecular weight substrate obtained from Neurospora
-
-
?
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
additional information
-
0.05% polygalactosaminoglycan
-
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.4
-
sporeling-detaching activity
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 8
-
very low activity at pH 3 and 8
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
31000
-
gel filtration, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
monomer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2
-
20°C, inactivated within a few min
135716
4 - 7
-
citrate-phosphate buffer
135717
7
-
quite stable at neutrality
135716
TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
20
-
pH 2, inactivated within a few min
70
-
15 min, 60% loss of activity
100
-
half-life: 3 min
additional information
-
heat stability increases at less acid pH
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
repeated freezing and thawing, no loss of activity
-
STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 8 months, distilled water, stable to repeated freezing and thawing
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE