Information on EC 3.2.1.107 - protein-glucosylgalactosylhydroxylysine glucosidase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
3.2.1.107
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RECOMMENDED NAME
GeneOntology No.
protein-glucosylgalactosylhydroxylysine glucosidase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
protein alpha-D-glucosyl-(1->2)-beta-D-galactosyl-L-hydroxylysine + H2O = D-glucose + protein beta-D-galactosyl-L-hydroxylysine
show the reaction diagram
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-
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-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydrolysis of O-glycosyl bond
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-
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SYSTEMATIC NAME
IUBMB Comments
protein-alpha-D-glucosyl-(1->2)-beta-D-galactosyl-L-hydroxylysine glucohydrolase
Requires free, positively charged epsilon-amino group of hydroxylysine.
CAS REGISTRY NUMBER
COMMENTARY hide
72829-45-9
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
White Leghorns
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-
Manually annotated by BRENDA team
male albino rat Charles River CD strain
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-
Manually annotated by BRENDA team
Sprague-Dawley
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-
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine + H2O
alpha-D-glucose + beta-D-galactopyranosyl-L-hydroxylysine
show the reaction diagram
basement membrane
D-glucose + ?
show the reaction diagram
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-
-
?
isomaltose + H2O
?
show the reaction diagram
kojibiose + H2O
alpha-D-glucose
show the reaction diagram
maltose + H2O
alpha-D-glucose
show the reaction diagram
additional information
?
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-
-
-
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
D-glucono-1,5-lactone
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10 mM complete inhibition
glucosamine
iodoacetic acid
-
-
NaCl
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0.8 mM 62% inhibition
p-chloromercuribenzoate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2.2 - 5.9
2-O-Alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
highest activity in the kidneys of neonatal rats and decreases with age
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
4.4 - 4.7
-
-
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3.9 - 5.8
-
pH 3.9 and 5.8, 50% of maximal activity
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
65000
-
gel filtration
75000
-
SDS-PAGE
85000
-
SDS-PAGE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
15 mg/l bovine serum albumin stabilize
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE